Restriction map for
pFN21AB6527
(Linear) (Six-base) MAPSORT of: KIBB6527.seq Check: 3252 from: 1 to: 1095
KIBB6527.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 934 1095
Size: 934 161
AatII G_ACGT'C
Cuts at: 0 839 1095
Size: 839 256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 967 1039 1051 1095
Size: 631 336 72 12 44
Fragments arranged by size:
631 336 72 44 12
AfeI AGC'GCT
Cuts at: 0 121 1095
Size: 121 974
AleI CACnn'nnGTG
Cuts at: 0 455 1095
Size: 455 640
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 257 289 1095
Size: 257 32 806
Fragments arranged by size:
806 257 32
AvaI C'yCGr_G
Cuts at: 0 223 360 1095
Size: 223 137 735
Fragments arranged by size:
735 223 137
BamHI G'GATC_C
Cuts at: 0 301 1095
Size: 301 794
BanI G'GyrC_C
Cuts at: 0 47 356 497 770 1095
Size: 47 309 141 273 325
Fragments arranged by size:
325 309 273 141 47
BanII G_rGCy'C
Cuts at: 0 67 233 623 1095
Size: 67 166 390 472
Fragments arranged by size:
472 390 166 67
BbeI G_GCGC'C
Cuts at: 0 51 1095
Size: 51 1044
Bme1580I G_kGCm'C
Cuts at: 0 204 773 1095
Size: 204 569 322
Fragments arranged by size:
569 322 204
BmrI ACTGGGnnnn_n'
Cuts at: 0 198 1095
Size: 198 897
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1068 1095
Size: 1068 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1095
Size: 1033 62
BsaAI yAC'GTr
Cuts at: 0 726 1095
Size: 726 369
BsaHI Gr'CG_yC
Cuts at: 0 48 95 542 653 836 1095
Size: 48 47 447 111 183 259
Fragments arranged by size:
447 259 183 111 48 47
BseYI C'CCAG_C
Cuts at: 0 12 708 1064 1095
Size: 12 696 356 31
Fragments arranged by size:
696 356 31 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 1095
Size: 552 543
BsiHKAI G_wGCw'C
Cuts at: 0 188 623 734 1095
Size: 188 435 111 361
Fragments arranged by size:
435 361 188 111
BsmI GAATG_Cn'
Cuts at: 0 426 1095
Size: 426 669
Bsp1286I G_dGCh'C
Cuts at: 0 67 188 204 233 623 734 773 1095
Size: 67 121 16 29 390 111 39 322
Fragments arranged by size:
390 322 121 111 67 39 29 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 934 1095
Size: 934 161
BsrDI GCAATG_nn'
Cuts at: 0 171 1095
Size: 171 924
BsrFI r'CCGG_y
Cuts at: 0 41 56 424 440 677 773 1095
Size: 41 15 368 16 237 96 322
Fragments arranged by size:
368 322 237 96 41 16 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 474 1095
Size: 474 621
BstYI r'GATC_y
Cuts at: 0 301 1095
Size: 301 794
BtgI C'CryG_G
Cuts at: 0 77 1095
Size: 77 1018
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 884 1095
Size: 884 211
BtsI GCAGTG_nn'
Cuts at: 0 186 724 1095
Size: 186 538 371
Fragments arranged by size:
538 371 186
EaeI y'GGCC_r
Cuts at: 0 23 101 324 1095
Size: 23 78 223 771
Fragments arranged by size:
771 223 78 23
EarI CTCTTCn'nnn_
Cuts at: 0 238 832 1095
Size: 238 594 263
Fragments arranged by size:
594 263 238
EcoICRI GAG'CTC
Cuts at: 0 621 1095
Size: 621 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 967 1033 1039 1051 1095
Size: 631 336 66 6 12 44
Fragments arranged by size:
631 336 66 44 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 889 1095
Size: 889 206
EcoO109I rG'GnC_Cy
Cuts at: 0 471 668 792 1095
Size: 471 197 124 303
Fragments arranged by size:
471 303 197 124
HaeII r_GCGC'y
Cuts at: 0 51 123 1095
Size: 51 72 972
Fragments arranged by size:
972 72 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 862 894 1072 1095
Size: 862 32 178 23
Fragments arranged by size:
862 178 32 23
HincII GTy'rAC
Cuts at: 0 159 702 1095
Size: 159 543 393
Fragments arranged by size:
543 393 159
KasI G'GCGC_C
Cuts at: 0 47 1095
Size: 47 1048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1022 1095
Size: 71 951 73
Fragments arranged by size:
951 73 71
MscI TGG'CCA
Cuts at: 0 103 1095
Size: 103 992
MslI CAynn'nnrTG
Cuts at: 0 455 1095
Size: 455 640
MspA1I CmG'CkG
Cuts at: 0 374 495 1011 1095
Size: 374 121 516 84
Fragments arranged by size:
516 374 121 84
NaeI GCC'GGC
Cuts at: 0 426 442 679 1095
Size: 426 16 237 416
Fragments arranged by size:
426 416 237 16
NarI GG'CG_CC
Cuts at: 0 48 1095
Size: 48 1047
NgoMIV G'CCGG_C
Cuts at: 0 424 440 677 1095
Size: 424 16 237 418
Fragments arranged by size:
424 418 237 16
PmlI CAC'GTG
Cuts at: 0 726 1095
Size: 726 369
PstI C_TGCA'G
Cuts at: 0 853 1095
Size: 853 242
SacI G_AGCT'C
Cuts at: 0 623 1095
Size: 623 472
SapI GCTCTTCn'nnn_
Cuts at: 0 238 832 1095
Size: 238 594 263
Fragments arranged by size:
594 263 238
ScaI AGT'ACT
Cuts at: 0 259 1095
Size: 259 836
SexAI A'CCwGG_T
Cuts at: 0 503 1095
Size: 503 592
SfcI C'TryA_G
Cuts at: 0 849 1095
Size: 849 246
SfoI GGC'GCC
Cuts at: 0 49 1095
Size: 49 1046
SmaI CCC'GGG
Cuts at: 0 362 1095
Size: 362 733
StuI AGG'CCT
Cuts at: 0 895 1095
Size: 895 200
StyI C'CwwG_G
Cuts at: 0 98 467 1070 1095
Size: 98 369 603 25
Fragments arranged by size:
603 369 98 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4 586 1095
Size: 4 582 509
Fragments arranged by size:
582 509 4
TatI w'GTAC_w
Cuts at: 0 257 482 1095
Size: 257 225 613
Fragments arranged by size:
613 257 225
XmaI C'CCGG_G
Cuts at: 0 360 1095
Size: 360 735
XmnI GAAnn'nnTTC
Cuts at: 0 760 1095
Size: 760 335
ZraI GAC'GTC
Cuts at: 0 837 1095
Size: 837 258
Enzymes that do cut:
AarI AatII AcuI AfeI AleI AloI AvaI BamHI
BanI BanII BbeI Bme1580I BmrI BplI BpmI BsaAI
BsaHI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrFI BstXI BstYI BtgI BtgZI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV PmlI
PstI SacI SapI ScaI SexAI SfcI SfoI SmaI
StuI StyI TaqII TatI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaBI
BsaWI BsaXI BseRI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XhoI