Restriction map for
pFN21AB6473
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflIII AgeI AhdI Alw44I AlwI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I CelII
Cfr10I CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 607 CGCG CG^CG
AccIII 273 TCCGGA T^CCGGA
AflII 943 CTTAAG C^TTAAG
AlwNI 433 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 962 CCTTAGG CC^T[ACGT]AGG
Aor51HI 32 AGCGCT AGC^GCT
AvaII 567 GGTCC G^G[AT]CC
BfaI 952 CTAG C^TAG
BfrI 943 CTTAAG C^TTAAG
BsaWI 273 TCCGGA [AT]^CCGG[AT]
BseAI 273 TCCGGA T^CCGGA
BsmFI 568 GTCCC GGGAC(10/14)
BsmI 333 GAATGC GAATGC(1/-1)
BspDI 424 ATCGAT AT^CGAT
BspEI 273 TCCGGA T^CCGGA
BspMI 838 ACCTGC ACCTGC(4/8)
BsrI 892 CCAGT ACTGG(1/-1)
BstUI 607 CGCG CG^CG
Bsu36I 962 CCTTAGG CC^T[ACGT]AGG
ClaI 424 ATCGAT AT^CGAT
DpnII 66 GATC ^GATC
DraII 566 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 344 GACACCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 145 CTCTTC CTCTTC(1/4)
Eco47III 32 AGCGCT AGC^GCT
Eco81I 962 CCTTAGG CC^T[ACGT]AGG
EcoO109I 566 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 226 GAATTC G^AATTC
EcoRV 316 GATATC GAT^ATC
EcoT14I 387 CCTTGG C^C[AT][AT]GG
HapII 274 CCGG C^CGG
HinfI 143 GACTC G^A[ACGT]TC
HpaII 274 CCGG C^CGG
Ksp632I 145 CTCTTC CTCTTC(1/4)
MaeI 952 CTAG C^TAG
MaeIII 290 GTGAC ^GT[ACGT]AC
MboI 66 GATC ^GATC
MroI 273 TCCGGA T^CCGGA
MspI 274 CCGG C^CGG
MvnI 607 CGCG CG^CG
NdeII 66 GATC ^GATC
NspI 636 GCATGT [GA]CATG^[CT]
PleI 143 GACTC GAGTC(4/5)
PpuMI 566 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 264 CAGCTG CAG^CTG
Sau3AI 66 GATC ^GATC
ScaI 170 AGTACT AGT^ACT
StyI 387 CCTTGG C^C[AT][AT]GG
Tsp45I 290 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 226 GAATTC [GA]^AATT[CT]
916 AAATTT
AfaI 90 GTAC GT^AC
171 GTAC
ApoI 226 GAATTC [GA]^AATT[CT]
916 AAATTT
BalI 2 TGGCCA TGG^CCA
14 TGGCCA
BbvI 432 GCAGC GCAGC(8/12)
35 GCTGC
BsaI 958 GAGACC GGTCTC(1/5)
789 GGTCTC
BsaJI 11 CCCTGG C^C[ACGT][ACGT]GG
387 CCTTGG
BstF5I 271 CATCC GGATG(2/0)
463 CATCC
BstXI 5 CCAACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
735 CCAGCTATTTGG
Cfr13I 567 GGTCC G^G[ACGT]CC
593 GGCCC
EcoNI 294 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
962 CCTTAGGAAGG
FauI 55 CCCGC CCCGC(4/6)
491 GCGGG
FokI 271 CATCC GGATG(9/13)
463 CATCC
HaeII 19 AGCGCC [GA]GCGC^[CT]
32 AGCGCT
MluNI 2 TGGCCA TGG^CCA
14 TGGCCA
MscI 2 TGGCCA TGG^CCA
14 TGGCCA
MslI 214 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
364 CATAATCGTG
NlaIV 566 GGGTCC GG[ACGT]^[ACGT]CC
567 GGTCCC
RsaI 90 GTAC GT^AC
171 GTAC
Sau96I 567 GGTCC G^G[ACGT]CC
593 GGCCC
SmlI 859 CTCAAG C^T[CT][GA]AG
943 CTTAAG
TaqI 425 TCGA T^CGA
599 TCGA
TseI 35 GCTGC G^C[AT]GC
432 GCAGC
TthHB8I 425 TCGA T^CGA
599 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI BsiYI BslI BsmAI
BssKI BstNI Cac8I CfoI DdeI
EaeI EcoRII Fnu4HI HaeIII HhaI
HinP1I HphI ItaI MboII MnlI
MseI MspA1I MvaI MwoI NlaIII
ScrFI SfaNI StuI Tru9I Tsp509I
TspRI