Restriction map for
pFN21AB6473
(Circular) (Six-base) MAPSORT of: pFN21AB6473.seq Check: 1918 from: 1 to: 5680
pFN21AB6473 5680 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2957 3743 5127 5395 1632
Size: 1325 786 1384 268 1917
Fragments arranged by size:
1917 1384 1325 786 268
AfeI AGC'GCT
Cuts at: 2039 2039
Size: 5680
AflII C'TTAA_G
Cuts at: 820 1017 2948 820
Size: 197 1931 3552
Fragments arranged by size:
3552 1931 197
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 2443 3423 4995 2443
Size: 980 1572 3128
Fragments arranged by size:
3128 1572 980
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2231 2921 2989 3215 3367 5476 1493
Size: 738 690 68 226 152 2109 1697
Fragments arranged by size:
2109 1697 738 690 226 152 68
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 3005 1870
Size: 73 1062 4545
Fragments arranged by size:
4545 1062 73
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3001 4456 618
Size: 325 217 627 1214 1455 1842
Fragments arranged by size:
1842 1455 1214 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2999 1391
Size: 1608 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 3741 4897 1790
Size: 1951 1156 2573
Fragments arranged by size:
2573 1951 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3411 4369 442
Size: 416 53 83 399 24 1994 958 1753
Fragments arranged by size:
1994 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4340 1239
Size: 3101 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3805 4670 4968 2849
Size: 956 865 298 3561
Fragments arranged by size:
3561 956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2800 2957 4343 882
Size: 617 1301 157 1386 2219
Fragments arranged by size:
2219 1386 1301 617 157
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2278 4113 4511 4785 4932 5343 2278
Size: 1835 398 274 147 411 2615
Fragments arranged by size:
2615 1835 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5680
BseYI C'CCAG_C
Cuts at: 2342 4883 5379 2342
Size: 2541 496 2643
Fragments arranged by size:
2643 2541 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3741 3826 4897 3741
Size: 85 1071 4524
Fragments arranged by size:
4524 1071 85
BsmI GAATG_Cn'
Cuts at: 2344 3191 3284 2344
Size: 847 93 4740
Fragments arranged by size:
4740 847 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2999 3741 3826 4897 1391
Size: 399 1209 742 85 1071 2174
Fragments arranged by size:
2174 1209 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2278 5343 2278
Size: 3065 2615
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2852 3035 844
Size: 2008 183 3489
Fragments arranged by size:
3489 2008 183
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4349 65
Size: 1183 2927 174 1396
Fragments arranged by size:
2927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5680
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2747 2017
Size: 730 4950
BstYI r'GATC_y
Cuts at: 1972 3010 3377 3762 3779 5231 5317 5329 5675
Size: 1038 367 385 17 1452 86 12 346
Cuts at: 5675 1972
Size: 1977
Fragments arranged by size:
1977 1452 1038 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2968 2968
Size: 5680
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4189
Fragments arranged by size:
4189 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2675 487
Size: 683 56 1449 3492
Fragments arranged by size:
3492 1449 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 2642 2764 3196 3990 4010 2111
Size: 531 122 432 794 20 3781
Fragments arranged by size:
3781 794 531 432 122 20
ClaI AT'CG_AT
Cuts at: 1554 2430 1554
Size: 876 4804
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2355 4687 809
Size: 507 1039 2332 1802
Fragments arranged by size:
2332 1802 1039 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2019 2242
Size: 54 1425 50 275 18 177 12 223
Cuts at: 2242 3127 4016 5645 8
Size: 885 889 1629 43
Fragments arranged by size:
1629 1425 889 885 275 223 177 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2156 3610 1353
Size: 803 1454 3423
Fragments arranged by size:
3423 1454 803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2957 3743 4340 5127 5395 1239
Size: 393 1325 786 597 787 268 1524
Fragments arranged by size:
1524 1325 787 786 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2303 2971 2303
Size: 668 5012
EcoO109I rG'GnC_Cy
Cuts at: 2572 2572
Size: 5680
EcoRI G'AATT_C
Cuts at: 2231 2989 3367 2231
Size: 758 378 4544
Fragments arranged by size:
4544 758 378
EcoRV GAT'ATC
Cuts at: 2323 2323
Size: 5680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 5680
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2028 2041 4827 1678
Size: 350 13 2786 2531
Fragments arranged by size:
2786 2531 350 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2586 2618 2780 2812 4321
Size: 32 135 32 1014 32 162 32 1509
Cuts at: 4321 4353 4395 4427 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1509 1014 162 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4794 4978 1305
Size: 3489 184 2007
Fragments arranged by size:
3489 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2021 10
Size: 54 1425 343 177 12 3669
Fragments arranged by size:
3669 1425 343 177 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2223 2373 3638 3997 4156 518
Size: 648 1057 150 1265 359 159 2042
Fragments arranged by size:
2042 1265 1057 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2063 2271 2292 3096 3773 4921 5166 1606
Size: 457 208 21 804 677 1148 245 2120
Fragments arranged by size:
2120 1148 804 677 457 245 208 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4189
Fragments arranged by size:
4189 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2645 3038 4583 1132
Size: 97 1416 393 1545 2229
Fragments arranged by size:
2229 1545 1416 393 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2572 2572
Size: 5680
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2447 2479 2447
Size: 32 5648
PstI C_TGCA'G
Cuts at: 830 3032 830
Size: 2202 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2271 2271
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SanDI GG'GwC_CC
Cuts at: 2572 2572
Size: 5680
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 2177 3928 5640 2177
Size: 1751 1712 2217
Fragments arranged by size:
2217 1751 1712
SfcI C'TryA_G
Cuts at: 826 1045 3028 4163 4844 5035 826
Size: 219 1983 1135 681 191 1471
Fragments arranged by size:
1983 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2864 2948 3784 4685 4947 820
Size: 197 926 921 84 836 901 262 1553
Fragments arranged by size:
1553 926 921 901 836 262 197 84
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 2391 2712 2813 1517
Size: 874 321 101 4384
Fragments arranged by size:
4384 874 321 101
StyI C'CwwG_G
Cuts at: 513 1065 2004 2392 3119 513
Size: 552 939 388 727 3074
Fragments arranged by size:
3074 939 727 552 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3707 3724 3877 4062 4507 325
Size: 1255 2127 17 153 185 445 1498
Fragments arranged by size:
2127 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 3926 5638
Size: 274 80 33 51 1443 198 1751 1712
Cuts at: 5638 96
Size: 138
Fragments arranged by size:
1751 1712 1443 274 198 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI