Restriction map for
pFN21AB6473
(Linear) (Six-base) MAPSORT of: KIBB6473.seq Check: 367 from: 1 to: 975
KIBB6473.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 975
Size: 952 23
AfeI AGC'GCT
Cuts at: 0 34 975
Size: 34 941
AflII C'TTAA_G
Cuts at: 0 943 975
Size: 943 32
AlwNI CAG_nnn'CTG
Cuts at: 0 438 975
Size: 438 537
ApoI r'AATT_y
Cuts at: 0 226 916 975
Size: 226 690 59
Fragments arranged by size:
690 226 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 975
Size: 844 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 795 952 975
Size: 795 157 23
Fragments arranged by size:
795 157 23
BsaWI w'CCGG_w
Cuts at: 0 273 975
Size: 273 702
BseYI C'CCAG_C
Cuts at: 0 337 975
Size: 337 638
BsmI GAATG_Cn'
Cuts at: 0 339 975
Size: 339 636
BspEI T'CCGG_A
Cuts at: 0 273 975
Size: 273 702
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 847 975
Size: 847 128
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 742 975
Size: 12 730 233
Fragments arranged by size:
730 233 12
Bsu36I CC'TnA_GG
Cuts at: 0 963 975
Size: 963 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 670 975
Size: 670 305
BtsI GCAGTG_nn'
Cuts at: 0 106 637 759 975
Size: 106 531 122 216
Fragments arranged by size:
531 216 122 106
ClaI AT'CG_AT
Cuts at: 0 425 975
Size: 425 550
DrdI GACnn_nn'nnGTC
Cuts at: 0 350 975
Size: 350 625
EaeI y'GGCC_r
Cuts at: 0 2 14 237 975
Size: 2 12 223 738
Fragments arranged by size:
738 223 12 2
EarI CTCTTCn'nnn_
Cuts at: 0 151 975
Size: 151 824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 975
Size: 952 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 298 966 975
Size: 298 668 9
Fragments arranged by size:
668 298 9
EcoO109I rG'GnC_Cy
Cuts at: 0 567 975
Size: 567 408
EcoRI G'AATT_C
Cuts at: 0 226 975
Size: 226 749
EcoRV GAT'ATC
Cuts at: 0 318 975
Size: 318 657
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 63 975
Size: 63 912
HaeII r_GCGC'y
Cuts at: 0 23 36 975
Size: 23 13 939
Fragments arranged by size:
939 23 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 581 613 775 807 975
Size: 581 32 162 32 168
Fragments arranged by size:
581 168 162 32 32
MscI TGG'CCA
Cuts at: 0 4 16 975
Size: 4 12 959
Fragments arranged by size:
959 12 4
MslI CAynn'nnrTG
Cuts at: 0 218 368 975
Size: 218 150 607
Fragments arranged by size:
607 218 150
MspA1I CmG'CkG
Cuts at: 0 58 266 287 975
Size: 58 208 21 688
Fragments arranged by size:
688 208 58 21
NspI r_CATG'y
Cuts at: 0 640 975
Size: 640 335
PpuMI rG'GwC_Cy
Cuts at: 0 567 975
Size: 567 408
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 442 474 975
Size: 442 32 501
Fragments arranged by size:
501 442 32
PvuII CAG'CTG
Cuts at: 0 266 975
Size: 266 709
SanDI GG'GwC_CC
Cuts at: 0 567 975
Size: 567 408
ScaI AGT'ACT
Cuts at: 0 172 975
Size: 172 803
SmlI C'TyrA_G
Cuts at: 0 859 943 975
Size: 859 84 32
Fragments arranged by size:
859 84 32
StuI AGG'CCT
Cuts at: 0 386 707 808 975
Size: 386 321 101 167
Fragments arranged by size:
386 321 167 101
StyI C'CwwG_G
Cuts at: 0 387 975
Size: 387 588
TatI w'GTAC_w
Cuts at: 0 170 975
Size: 170 805
Enzymes that do cut:
AcuI AfeI AflII AlwNI ApoI BpuEI BsaI BsaWI
BseYI BsmI BspEI BspMI BstXI Bsu36I BtgZI BtsI
ClaI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I MscI MslI MspA1I NspI
PpuMI PsrI PvuII SanDI ScaI SmlI StuI StyI
TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI CspCI
DraI DraIII EagI EciI EcoICRI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI