################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbsI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 669 GACGTC GACGT^C Alw44I 988 GTGCAC G^TGCAC AlwI 228 GATCC GGATC(4/5) ApaLI 988 GTGCAC G^TGCAC BbeI 861 GGCGCC GGCGC^C BbrPI 271 CACGTG CAC^GTG BcgI 619 GCAGTGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 210 CTCCAG CTGGAG(16/14) BsaAI 271 CACGTG [CT]AC^GT[GA] BsgI 949 CTGCAC GTGCAG(16/14) BsiEI 402 CGGCCG CG[GA][CT]^CG BssSI 480 CTCGTG CACGAG(-5/-1) BstAPI 693 GCAGGTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 942 GGTCACC G^GT[ACGT]ACC BstPI 942 GGTCACC G^GT[ACGT]ACC DraII 86 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 841 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 402 CGGCCG C^GGCCG EclXI 402 CGGCCG C^GGCCG Eco52I 402 CGGCCG C^GGCCG EcoNI 519 CCTGGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 86 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 942 GGTCACC G^GT[ACGT]ACC EcoRV 239 GATATC GAT^ATC EcoT14I 65 CCATGG C^C[AT][AT]GG KasI 861 GGCGCC G^GCGCC KspI 680 CCGCGG CCGC^GG MaeIII 943 GTCAC ^GT[ACGT]AC MboII 350 TCTTC GAAGA(8/7) MslI 786 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 861 GGCGCC GG^CGCC NcoI 65 CCATGG C^CATGG PflMI 419 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 271 CACGTG CAC^GTG PmlI 271 CACGTG CAC^GTG PshAI 112 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 680 CCGCGG CCGC^GG SfaNI 545 GCATC GCATC(5/9) SfoI 861 GGCGCC GGC^GCC StuI 122 AGGCCT AGG^CCT StyI 65 CCATGG C^C[AT][AT]GG TfiI 377 GATTC G^A[AT]TC Tsp45I 943 GTCAC ^GT[GC]AC Van91I 419 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 492 CCAGCTGTCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BanI 278 GGTGCC G^G[CT][GA]CC 861 GGCGCC BanII 98 GAGCTC G[GA]GC[CT]^C 437 GAGCTC BbvCI 771 CCTCAGC CCTCAGC(-5/-2) 309 GCTGAGG BglII 347 AGATCT A^GATCT 410 AGATCT BspMI 693 GCAGGT ACCTGC(4/8) 984 GCAGGT BsrBI 1048 CCGCTC CCGCTC(-3/-3) 928 GAGCGG BsrFI 651 GCCGGC [GA]^CCGG[CT] 995 GCCGGC Cfr10I 651 GCCGGC [GA]^CCGG[CT] 995 GCCGGC DsaI 65 CCATGG C^C[GA][CT]GG 680 CCGCGG EaeI 402 CGGCCG [CT]^GGCC[GA] 653 CGGCCA HaeII 488 AGCGCC [GA]GCGC^[CT] 861 GGCGCC HincII 253 GTCAAC GT[CT]^[GA]AC 641 GTCAAC HindII 253 GTCAAC GT[CT]^[GA]AC 641 GTCAAC NaeI 651 GCCGGC GCC^GGC 995 GCCGGC NgoMIV 651 GCCGGC G^CCGGC 995 GCCGGC PaeR7I 223 CTCGAG C^TCGAG 283 CTCGAG PleI 782 GAGTC GAGTC(4/5) 966 GAGTC SacI 98 GAGCTC GAGCT^C 437 GAGCTC XhoI 223 CTCGAG C^TCGAG 283 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AspHI AvaI AvaII BbvI BcnI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MboI MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI SmlI TaqI TseI TspRI TthHB8I XhoII