(Circular) (Six-base) MAPSORT of: pFN21AE3681.seq Check: 7718 from: 1 to: 5794 pFN21AE3681 5794 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2980 2980 Size: 5794 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2678 3604 278 Size: 53 83 186 936 1142 926 2468 Fragments arranged by size: 2468 1142 936 926 186 83 53 AccI GT'mk_AC Cuts at: 1937 3137 1937 Size: 1200 4594 Acc65I G'GTAC_C Cuts at: 3115 3115 Size: 5794 AclI AA'CG_TT Cuts at: 1563 3921 4294 1563 Size: 2358 373 3063 Fragments arranged by size: 3063 2358 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2359 3857 5241 5509 1632 Size: 727 1498 1384 268 1917 Fragments arranged by size: 1917 1498 1384 727 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5597 AflIII A'CryG_T Cuts at: 1203 4693 5670 1203 Size: 3490 977 1327 Fragments arranged by size: 3490 1327 977 AgeI A'CCGG_T Cuts at: 4625 4625 Size: 5794 AhdI GACnn_n'nnGTC Cuts at: 4523 4523 Size: 5794 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5794 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5794 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2198 2230 2198 Size: 32 5762 AlwNI CAG_nnn'CTG Cuts at: 2430 2749 2758 3537 5109 2430 Size: 319 9 779 1572 3115 Fragments arranged by size: 3115 1572 779 319 9 ApaLI G'TGCA_C Cuts at: 2993 3851 5007 2993 Size: 858 1156 3780 Fragments arranged by size: 3780 1156 858 ApoI r'AATT_y Cuts at: 1493 3103 3329 3481 5590 1493 Size: 1610 226 152 2109 1697 Fragments arranged by size: 2109 1697 1610 226 152 AseI AT'TA_AT Cuts at: 160 1032 4348 160 Size: 872 3316 1606 Fragments arranged by size: 3316 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 AvaI C'yCGr_G Cuts at: 1870 1943 2228 2288 2860 2930 3119 1870 Size: 73 285 60 572 70 189 4545 Fragments arranged by size: 4545 572 285 189 73 70 60 BamHI G'GATC_C Cuts at: 3124 3491 3124 Size: 367 5427 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2283 2866 3115 4570 618 Size: 325 217 627 496 583 249 1455 1842 Fragments arranged by size: 1842 1455 627 583 496 325 249 217 BanII G_rGCy'C Cuts at: 1391 2107 2446 3113 1391 Size: 716 339 667 4072 Fragments arranged by size: 4072 716 667 339 BbeI G_GCGC'C Cuts at: 2870 2870 Size: 5794 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4823 BbvCI CC'TCA_GC Cuts at: 2315 2777 2315 Size: 462 5332 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2613 2647 3985 4019 1903 Size: 34 676 34 1338 34 3678 Fragments arranged by size: 3678 1338 676 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3686 4896 3686 Size: 1210 4584 BclI T'GATC_A Cuts at: 1548 1548 Size: 5794 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4405 136 Size: 107 122 71 631 605 2733 1525 Fragments arranged by size: 2733 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2352 2415 5789 2352 Size: 63 3374 2357 Fragments arranged by size: 3374 2357 63 BlpI GC'TnA_GC Cuts at: 3211 3211 Size: 5794 Bme1580I G_kGCm'C Cuts at: 1790 2401 2631 2997 3855 5011 1790 Size: 611 230 366 858 1156 2573 Fragments arranged by size: 2573 1156 858 611 366 230 BmgBI CAC'GTC Cuts at: 1318 2310 1318 Size: 992 4802 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2440 2843 3525 Size: 416 53 83 399 24 1023 403 682 Cuts at: 3525 4483 442 Size: 958 1753 Fragments arranged by size: 1753 1023 958 682 416 403 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2200 4454 1239 Size: 961 2254 2579 Fragments arranged by size: 2579 2254 961 Bpu10I CC'TnA_GC Cuts at: 2315 2777 2315 Size: 462 5332 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2237 2564 3919 4784 5082 2237 Size: 327 1355 865 298 2949 Fragments arranged by size: 2949 1355 865 327 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4457 882 Size: 617 2958 2219 Fragments arranged by size: 2958 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2278 3585 493 Size: 711 1074 1307 2702 Fragments arranged by size: 2702 1307 1074 711 BsaBI GATnn'nnATC Cuts at: 5788 5788 Size: 5794 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 2675 2867 3601 Size: 53 83 186 936 552 590 192 734 Cuts at: 3601 3983 4618 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 734 635 590 552 382 192 186 83 53 BsaWI w'CCGG_w Cuts at: 4227 4625 4899 5046 5457 4227 Size: 398 274 147 411 4564 Fragments arranged by size: 4564 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2198 2228 1178 Size: 30 165 30 795 30 4744 Fragments arranged by size: 4744 795 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2173 2528 2546 1186 Size: 987 355 18 4434 Fragments arranged by size: 4434 987 355 18 BseYI C'CCAG_C Cuts at: 2010 2144 4997 5493 2010 Size: 134 2853 496 2311 Fragments arranged by size: 2853 2311 496 134 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2939 1163 Size: 573 1203 4018 Fragments arranged by size: 4018 1203 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2410 3244 4005 5033 1540 Size: 275 186 409 834 761 1028 2301 Fragments arranged by size: 2301 1028 834 761 409 275 186 BsiHKAI G_wGCw'C Cuts at: 2107 2297 2446 2544 2997 3855 3940 5011 2107 Size: 190 149 98 453 858 85 1071 2890 Fragments arranged by size: 2890 1071 858 453 190 149 98 85 BsmI GAATG_Cn' Cuts at: 3305 3398 3305 Size: 93 5701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 2297 2401 2446 2544 2631 2997 Size: 399 317 190 104 45 98 87 366 Cuts at: 2997 3113 3855 3940 5011 1391 Size: 116 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 317 190 116 104 98 87 85 45 BspEI T'CCGG_A Cuts at: 5457 5457 Size: 5794 BspHI T'CATG_A Cuts at: 3683 5413 3683 Size: 1730 4064 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2689 2980 3149 844 Size: 1845 291 169 3489 Fragments arranged by size: 3489 1845 291 169 BsrBI CCG'CTC Cuts at: 1394 1823 2935 3055 3681 1394 Size: 429 1112 120 626 3507 Fragments arranged by size: 3507 1112 626 429 120 BsrDI GCAATG_nn' Cuts at: 65 1248 4289 4463 65 Size: 1183 3041 174 1396 Fragments arranged by size: 3041 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2656 3000 4438 4625 1700 Size: 110 846 344 1438 187 2869 Fragments arranged by size: 2869 1438 846 344 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5794 BssHII G'CGCG_C Cuts at: 1925 2665 2667 2939 1925 Size: 740 2 272 4780 Fragments arranged by size: 4780 740 272 2 BssSI C'ACGA_G Cuts at: 2485 3854 4866 2485 Size: 1369 1012 3413 Fragments arranged by size: 3413 1369 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2704 794 Size: 1910 3884 BstBI TT'CG_AA Cuts at: 4598 4598 Size: 5794 BstEII G'GTnAC_C Cuts at: 1184 2947 1184 Size: 1763 4031 BstYI r'GATC_y Cuts at: 1972 2232 2352 2415 3124 3491 3876 3893 5345 Size: 260 120 63 709 367 385 17 1452 Cuts at: 5345 5431 5443 5789 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 709 385 367 346 260 120 86 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2685 513 Size: 552 939 66 615 3622 Fragments arranged by size: 3622 939 615 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5055 Fragments arranged by size: 5055 683 56 BtsI GCAGTG_nn' Cuts at: 2631 2995 3310 4104 4124 2631 Size: 364 315 794 20 4301 Fragments arranged by size: 4301 794 364 315 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5794 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5759 DraI TTT'AAA Cuts at: 3098 3098 Size: 5794 DraIII CAC_nnn'GTG Cuts at: 2851 2851 Size: 5794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4801 809 Size: 507 3485 1802 Fragments arranged by size: 3485 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2407 2658 3241 Size: 54 1425 50 275 18 577 251 583 Cuts at: 3241 4130 5759 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 583 577 275 251 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2407 3241 1537 Size: 275 595 834 4090 Fragments arranged by size: 4090 834 595 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3724 1353 Size: 2371 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2777 2878 4381 4755 4901 387 Size: 1064 138 1188 101 1503 374 146 1280 Fragments arranged by size: 1503 1280 1188 1064 374 146 138 101 EcoICRI GAG'CTC Cuts at: 2105 2444 2105 Size: 339 5455 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2200 2359 3857 4454 5241 5509 1239 Size: 393 568 159 1498 597 787 268 1524 Fragments arranged by size: 1524 1498 787 597 568 393 268 159 EcoNI CCTnn'n_nnAGG Cuts at: 2528 2528 Size: 5794 EcoO109I rG'GnC_Cy Cuts at: 2092 2092 Size: 5794 EcoRI G'AATT_C Cuts at: 3103 3481 3103 Size: 378 5416 EcoRV GAT'ATC Cuts at: 2246 2246 Size: 5794 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2371 2371 Size: 5794 FspI TGC'GCA Cuts at: 4300 4300 Size: 5794 HaeII r_GCGC'y Cuts at: 1678 2497 2870 4941 1678 Size: 819 373 2071 2531 Fragments arranged by size: 2531 2071 819 373 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2235 2267 4435 4467 4509 Size: 32 135 32 663 32 2168 32 42 Cuts at: 4509 4541 1373 Size: 32 2626 Fragments arranged by size: 2626 2168 663 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2260 2648 3138 3384 669 Size: 988 281 322 388 490 246 3079 Fragments arranged by size: 3079 988 490 388 322 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5794 HpaI GTT'AAC Cuts at: 3384 3384 Size: 5794 KasI G'GCGC_C Cuts at: 2866 2866 Size: 5794 KpnI G_GTAC'C Cuts at: 3119 3119 Size: 5794 MfeI C'AATT_G Cuts at: 3393 3393 Size: 5794 MluI A'CGCG_T Cuts at: 5670 5670 Size: 5794 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2595 2922 3084 4908 5092 1305 Size: 1290 327 162 1824 184 2007 Fragments arranged by size: 2007 1824 1290 327 184 162 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3972 Fragments arranged by size: 3972 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2795 3752 4111 4270 518 Size: 648 1629 957 359 159 2042 Fragments arranged by size: 2042 1629 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2043 2220 2331 2500 2687 2695 3210 3887 Size: 437 177 111 169 187 8 515 677 Cuts at: 3887 5035 5280 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 515 437 245 187 177 169 111 8 NaeI GCC'GGC Cuts at: 2658 3002 2658 Size: 344 5450 NarI GG'CG_CC Cuts at: 2867 2867 Size: 5794 NcoI C'CATG_G Cuts at: 513 1065 2004 2070 513 Size: 552 939 66 4237 Fragments arranged by size: 4237 939 552 66 NdeI CA'TA_TG Cuts at: 387 387 Size: 5794 NgoMIV G'CCGG_C Cuts at: 2656 3000 2656 Size: 344 5450 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5794 NotI GC'GGCC_GC Cuts at: 3241 3241 Size: 5794 NspI r_CATG'y Cuts at: 1132 1229 3152 4697 1132 Size: 97 1923 1545 2229 Fragments arranged by size: 2229 1923 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5794 PciI A'CATG_T Cuts at: 4693 4693 Size: 5794 PflMI CCAn_nnn'nTGG Cuts at: 1108 2430 1108 Size: 1322 4472 PfoI T'CCnGG_A Cuts at: 2235 2235 Size: 5794 PmeI GTTT'AAAC Cuts at: 3098 3098 Size: 5794 PmlI CAC'GTG Cuts at: 2278 2278 Size: 5794 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2198 2230 3863 3895 5376 5408 2198 Size: 32 1633 32 1481 32 2584 Fragments arranged by size: 2584 1633 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2121 1613 Size: 508 5286 PsiI TTA'TAA Cuts at: 3364 3364 Size: 5794 PspXI vC'TCGA_Gb Cuts at: 2288 2288 Size: 5794 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2318 2350 2318 Size: 32 5762 PstI C_TGCA'G Cuts at: 830 2200 2700 2754 3146 830 Size: 1370 500 54 392 3478 Fragments arranged by size: 3478 1370 500 392 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5794 PvuII CAG'CTG Cuts at: 2220 2331 2500 2695 2220 Size: 111 169 195 5319 Fragments arranged by size: 5319 195 169 111 SacI G_AGCT'C Cuts at: 2107 2446 2107 Size: 339 5455 SacII CC_GC'GG Cuts at: 2688 2688 Size: 5794 SalI G'TCGA_C Cuts at: 1936 3136 1936 Size: 1200 4594 SbfI CC_TGCA'GG Cuts at: 3146 3146 Size: 5794 ScaI AGT'ACT Cuts at: 4042 5754 4042 Size: 1712 4082 SfcI C'TryA_G Cuts at: 826 1045 2196 2696 2750 3142 4277 4958 5149 Size: 219 1151 500 54 392 1135 681 191 Cuts at: 5149 826 Size: 1471 Fragments arranged by size: 1471 1151 1135 681 500 392 219 191 54 SfoI GGC'GCC Cuts at: 2868 2868 Size: 5794 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5794 SmaI CCC'GGG Cuts at: 1872 3121 1872 Size: 1249 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2228 2252 2288 2579 3898 4799 Size: 197 926 285 24 36 291 1319 901 Cuts at: 4799 5061 820 Size: 262 1553 Fragments arranged by size: 1553 1319 926 901 291 285 262 197 36 24 SnaBI TAC'GTA Cuts at: 493 3585 493 Size: 3092 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5794 SphI G_CATG'C Cuts at: 1132 3152 1132 Size: 2020 3774 SspI AAT'ATT Cuts at: 5 52 3718 5 Size: 47 3666 2081 Fragments arranged by size: 3666 2081 47 StuI AGG'CCT Cuts at: 1517 2129 1517 Size: 612 5182 StyI C'CwwG_G Cuts at: 513 1065 2004 2070 3233 513 Size: 552 939 66 1163 3074 Fragments arranged by size: 3074 1163 939 552 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2256 2422 2862 3821 3838 3991 4176 Size: 1255 676 166 440 959 17 153 185 Cuts at: 4176 4621 325 Size: 445 1498 Fragments arranged by size: 1498 1255 959 676 445 440 185 166 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4040 5752 96 Size: 274 80 33 51 1443 2063 1712 138 Fragments arranged by size: 2063 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2380 2412 3009 3041 5754 5786 2380 Size: 32 597 32 2713 32 2388 Fragments arranged by size: 2713 2388 597 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5794 XbaI T'CTAG_A Cuts at: 3130 3130 Size: 5794 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2504 2504 Size: 5794 XhoI C'TCGA_G Cuts at: 1943 2228 2288 1943 Size: 285 60 5449 Fragments arranged by size: 5449 285 60 XmaI C'CCGG_G Cuts at: 1870 3119 1870 Size: 1249 4545 XmnI GAAnn'nnTTC Cuts at: 3923 3923 Size: 5794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2676 3602 276 Size: 53 83 186 936 1142 926 2468 Fragments arranged by size: 2468 1142 936 926 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I FseI FspAI NruI NsiI PacI PpuMI PspOMI RsrII SanDI SapI SexAI SfiI SrfI SwaI