(Linear) (Six-base) MAPSORT of: KIEE3681.seq Check: 5176 from: 1 to: 1089 KIEE3681.orf With 182 enzymes: SgfI * July 3, 2012 18:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 975 1089 Size: 975 114 AatII G_ACGT'C Cuts at: 0 673 1089 Size: 673 416 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 354 1089 Size: 354 735 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 193 225 1089 Size: 193 32 864 Fragments arranged by size: 864 193 32 AlwNI CAG_nnn'CTG Cuts at: 0 425 744 753 1089 Size: 425 319 9 336 Fragments arranged by size: 425 336 319 9 ApaLI G'TGCA_C Cuts at: 0 988 1089 Size: 988 101 AvaI C'yCGr_G Cuts at: 0 223 283 855 925 1089 Size: 223 60 572 70 164 Fragments arranged by size: 572 223 164 70 60 BanI G'GyrC_C Cuts at: 0 278 861 1089 Size: 278 583 228 Fragments arranged by size: 583 278 228 BanII G_rGCy'C Cuts at: 0 102 441 1089 Size: 102 339 648 Fragments arranged by size: 648 339 102 BbeI G_GCGC'C Cuts at: 0 865 1089 Size: 865 224 BbvCI CC'TCA_GC Cuts at: 0 310 772 1089 Size: 310 462 317 Fragments arranged by size: 462 317 310 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 608 642 1089 Size: 608 34 447 Fragments arranged by size: 608 447 34 BglII A'GATC_T Cuts at: 0 347 410 1089 Size: 347 63 679 Fragments arranged by size: 679 347 63 Bme1580I G_kGCm'C Cuts at: 0 396 626 992 1089 Size: 396 230 366 97 Fragments arranged by size: 396 366 230 97 BmgBI CAC'GTC Cuts at: 0 305 1089 Size: 305 784 BmrI ACTGGGnnnn_n' Cuts at: 0 435 838 1089 Size: 435 403 251 Fragments arranged by size: 435 403 251 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 195 1089 Size: 195 894 Bpu10I CC'TnA_GC Cuts at: 0 310 772 1089 Size: 310 462 317 Fragments arranged by size: 462 317 310 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 559 1089 Size: 232 327 530 Fragments arranged by size: 530 327 232 BsaAI yAC'GTr Cuts at: 0 273 1089 Size: 273 816 BsaHI Gr'CG_yC Cuts at: 0 80 670 862 1089 Size: 80 590 192 227 Fragments arranged by size: 590 227 192 80 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 193 223 1089 Size: 193 30 866 Fragments arranged by size: 866 193 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 168 523 541 1089 Size: 168 355 18 548 Fragments arranged by size: 548 355 168 18 BseYI C'CCAG_C Cuts at: 0 5 139 1089 Size: 5 134 950 Fragments arranged by size: 950 134 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 934 1089 Size: 934 155 BsiEI CG_ry'CG Cuts at: 0 405 1089 Size: 405 684 BsiHKAI G_wGCw'C Cuts at: 0 102 292 441 539 992 1089 Size: 102 190 149 98 453 97 Fragments arranged by size: 453 190 149 102 98 97 Bsp1286I G_dGCh'C Cuts at: 0 102 292 396 441 539 626 992 1089 Size: 102 190 104 45 98 87 366 97 Fragments arranged by size: 366 190 104 102 98 97 87 45 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 684 975 1089 Size: 684 291 114 Fragments arranged by size: 684 291 114 BsrBI CCG'CTC Cuts at: 0 930 1050 1089 Size: 930 120 39 Fragments arranged by size: 930 120 39 BsrFI r'CCGG_y Cuts at: 0 651 995 1089 Size: 651 344 94 Fragments arranged by size: 651 344 94 BssHII G'CGCG_C Cuts at: 0 660 662 934 1089 Size: 660 2 272 155 Fragments arranged by size: 660 272 155 2 BssSI C'ACGA_G Cuts at: 0 480 1089 Size: 480 609 BstAPI GCAn_nnn'nTGC Cuts at: 0 699 1089 Size: 699 390 BstEII G'GTnAC_C Cuts at: 0 942 1089 Size: 942 147 BstYI r'GATC_y Cuts at: 0 227 347 410 1089 Size: 227 120 63 679 Fragments arranged by size: 679 227 120 63 BtgI C'CryG_G Cuts at: 0 65 680 1089 Size: 65 615 409 Fragments arranged by size: 615 409 65 BtsI GCAGTG_nn' Cuts at: 0 626 990 1089 Size: 626 364 99 Fragments arranged by size: 626 364 99 DraIII CAC_nnn'GTG Cuts at: 0 846 1089 Size: 846 243 EaeI y'GGCC_r Cuts at: 0 402 653 1089 Size: 402 251 436 Fragments arranged by size: 436 402 251 EagI C'GGCC_G Cuts at: 0 402 1089 Size: 402 687 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 772 873 1089 Size: 772 101 216 Fragments arranged by size: 772 216 101 EcoICRI GAG'CTC Cuts at: 0 100 439 1089 Size: 100 339 650 Fragments arranged by size: 650 339 100 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 195 354 1089 Size: 195 159 735 Fragments arranged by size: 735 195 159 EcoNI CCTnn'n_nnAGG Cuts at: 0 523 1089 Size: 523 566 EcoO109I rG'GnC_Cy Cuts at: 0 87 1089 Size: 87 1002 EcoRV GAT'ATC Cuts at: 0 241 1089 Size: 241 848 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 366 1089 Size: 366 723 HaeII r_GCGC'y Cuts at: 0 492 865 1089 Size: 492 373 224 Fragments arranged by size: 492 373 224 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 230 262 1089 Size: 230 32 827 Fragments arranged by size: 827 230 32 HincII GTy'rAC Cuts at: 0 255 643 1089 Size: 255 388 446 Fragments arranged by size: 446 388 255 KasI G'GCGC_C Cuts at: 0 861 1089 Size: 861 228 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 590 917 1079 1089 Size: 590 327 162 10 Fragments arranged by size: 590 327 162 10 MslI CAynn'nnrTG Cuts at: 0 790 1089 Size: 790 299 MspA1I CmG'CkG Cuts at: 0 38 215 326 495 682 690 1089 Size: 38 177 111 169 187 8 399 Fragments arranged by size: 399 187 177 169 111 38 8 NaeI GCC'GGC Cuts at: 0 653 997 1089 Size: 653 344 92 Fragments arranged by size: 653 344 92 NarI GG'CG_CC Cuts at: 0 862 1089 Size: 862 227 NcoI C'CATG_G Cuts at: 0 65 1089 Size: 65 1024 NgoMIV G'CCGG_C Cuts at: 0 651 995 1089 Size: 651 344 94 Fragments arranged by size: 651 344 94 PflMI CCAn_nnn'nTGG Cuts at: 0 425 1089 Size: 425 664 PfoI T'CCnGG_A Cuts at: 0 230 1089 Size: 230 859 PmlI CAC'GTG Cuts at: 0 273 1089 Size: 273 816 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 193 225 1089 Size: 193 32 864 Fragments arranged by size: 864 193 32 PshAI GACnn'nnGTC Cuts at: 0 116 1089 Size: 116 973 PspXI vC'TCGA_Gb Cuts at: 0 283 1089 Size: 283 806 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 313 345 1089 Size: 313 32 744 Fragments arranged by size: 744 313 32 PstI C_TGCA'G Cuts at: 0 195 695 749 1089 Size: 195 500 54 340 Fragments arranged by size: 500 340 195 54 PvuII CAG'CTG Cuts at: 0 215 326 495 690 1089 Size: 215 111 169 195 399 Fragments arranged by size: 399 215 195 169 111 SacI G_AGCT'C Cuts at: 0 102 441 1089 Size: 102 339 648 Fragments arranged by size: 648 339 102 SacII CC_GC'GG Cuts at: 0 683 1089 Size: 683 406 SfcI C'TryA_G Cuts at: 0 191 691 745 1089 Size: 191 500 54 344 Fragments arranged by size: 500 344 191 54 SfoI GGC'GCC Cuts at: 0 863 1089 Size: 863 226 SmlI C'TyrA_G Cuts at: 0 223 247 283 574 1089 Size: 223 24 36 291 515 Fragments arranged by size: 515 291 223 36 24 StuI AGG'CCT Cuts at: 0 124 1089 Size: 124 965 StyI C'CwwG_G Cuts at: 0 65 1089 Size: 65 1024 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 251 417 857 1089 Size: 251 166 440 232 Fragments arranged by size: 440 251 232 166 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 375 407 1004 1036 1089 Size: 375 32 597 32 53 Fragments arranged by size: 597 375 53 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 499 1089 Size: 499 590 XhoI C'TCGA_G Cuts at: 0 223 283 1089 Size: 223 60 806 Fragments arranged by size: 806 223 60 ZraI GAC'GTC Cuts at: 0 671 1089 Size: 671 418 Enzymes that do cut: AarI AatII AcuI AloI AlwNI ApaLI AvaI BanI BanII BbeI BbvCI BcgI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstYI BtgI BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PflMI PfoI PmlI PpiI PshAI PspXI PsrI PstI PvuII SacI SacII SfcI SfoI SmlI StuI StyI TaqII TstI XcmI XhoI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglI BlpI BmtI BplI BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI EcoRI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PpuMI PsiI PspOMI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XmaI XmnI