Restriction map for
pFN21AE0375
(Linear) (Six-base) MAPSORT of: KIEE0375.seq Check: 6483 from: 1 to: 1701
KIEE0375.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 366 1701
Size: 366 1335
AatII G_ACGT'C
Cuts at: 0 203 1701
Size: 203 1498
AccI GT'mk_AC
Cuts at: 0 1354 1701
Size: 1354 347
Acc65I G'GTAC_C
Cuts at: 0 1535 1629 1701
Size: 1535 94 72
Fragments arranged by size:
1535 94 72
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 676 1451 1701
Size: 516 160 775 250
Fragments arranged by size:
775 516 250 160
AgeI A'CCGG_T
Cuts at: 0 963 1701
Size: 963 738
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 1701
Size: 1048 32 621
Fragments arranged by size:
1048 621 32
ApaI G_GGCC'C
Cuts at: 0 1079 1701
Size: 1079 622
ApoI r'AATT_y
Cuts at: 0 397 770 1249 1283 1429 1701
Size: 397 373 479 34 146 272
Fragments arranged by size:
479 397 373 272 146 34
AvaI C'yCGr_G
Cuts at: 0 605 1701
Size: 605 1096
BanI G'GyrC_C
Cuts at: 0 45 160 667 1535 1629 1701
Size: 45 115 507 868 94 72
Fragments arranged by size:
868 507 115 94 72 45
BanII G_rGCy'C
Cuts at: 0 1079 1592 1701
Size: 1079 513 109
Fragments arranged by size:
1079 513 109
BbeI G_GCGC'C
Cuts at: 0 164 1701
Size: 164 1537
BbsI GAAGACnn'nnnn_
Cuts at: 0 195 1701
Size: 195 1506
BbvCI CC'TCA_GC
Cuts at: 0 298 1701
Size: 298 1403
BciVI GTATCCnnnnn_n'
Cuts at: 0 1294 1390 1701
Size: 1294 96 311
Fragments arranged by size:
1294 311 96
BglII A'GATC_T
Cuts at: 0 1131 1701
Size: 1131 570
Bme1580I G_kGCm'C
Cuts at: 0 50 1079 1701
Size: 50 1029 622
Fragments arranged by size:
1029 622 50
BmgBI CAC'GTC
Cuts at: 0 312 1701
Size: 312 1389
BmrI ACTGGGnnnn_n'
Cuts at: 0 474 798 1701
Size: 474 324 903
Fragments arranged by size:
903 474 324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 961 967 1701
Size: 469 492 6 734
Fragments arranged by size:
734 492 469 6
Bpu10I CC'TnA_GC
Cuts at: 0 298 1701
Size: 298 1403
BsaI GGTCTCn'nnnn_
Cuts at: 0 1370 1701
Size: 1370 331
BsaAI yAC'GTr
Cuts at: 0 740 1701
Size: 740 961
BsaHI Gr'CG_yC
Cuts at: 0 161 200 1701
Size: 161 39 1501
Fragments arranged by size:
1501 161 39
BsaWI w'CCGG_w
Cuts at: 0 963 1701
Size: 963 738
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 313 343 467 497 1048 1078 1664 1694
Size: 313 30 124 30 551 30 586 30
Cuts at: 1694 1701
Size: 7
Fragments arranged by size:
586 551 313 124 30 30 30 30
7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1672 1701
Size: 1672 29
BseYI C'CCAG_C
Cuts at: 0 54 1078 1701
Size: 54 1024 623
Fragments arranged by size:
1024 623 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 953 1701
Size: 292 661 748
Fragments arranged by size:
748 661 292
BsiEI CG_ry'CG
Cuts at: 0 1095 1701
Size: 1095 606
BsmI GAATG_Cn'
Cuts at: 0 723 1701
Size: 723 978
BsmBI CGTCTCn'nnnn_
Cuts at: 0 235 625 1701
Size: 235 390 1076
Fragments arranged by size:
1076 390 235
Bsp1286I G_dGCh'C
Cuts at: 0 50 1079 1592 1701
Size: 50 1029 513 109
Fragments arranged by size:
1029 513 109 50
BspHI T'CATG_A
Cuts at: 0 905 1701
Size: 905 796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 366 1701
Size: 366 1335
BsrDI GCAATG_nn'
Cuts at: 0 617 1493 1701
Size: 617 876 208
Fragments arranged by size:
876 617 208
BsrFI r'CCGG_y
Cuts at: 0 670 886 963 1701
Size: 670 216 77 738
Fragments arranged by size:
738 670 216 77
BsrGI T'GTAC_A
Cuts at: 0 677 1701
Size: 677 1024
BstEII G'GTnAC_C
Cuts at: 0 959 1701
Size: 959 742
BstYI r'GATC_y
Cuts at: 0 468 1131 1701
Size: 468 663 570
Fragments arranged by size:
663 570 468
BstZ17I GTA'TAC
Cuts at: 0 1355 1701
Size: 1355 346
Bsu36I CC'TnA_GG
Cuts at: 0 1687 1701
Size: 1687 14
BtgI C'CryG_G
Cuts at: 0 663 1162 1701
Size: 663 499 539
Fragments arranged by size:
663 539 499
BtsI GCAGTG_nn'
Cuts at: 0 1661 1701
Size: 1661 40
ClaI AT'CG_AT
Cuts at: 0 416 1701
Size: 416 1285
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 480 515 1505 1540 1701
Size: 480 35 990 35 161
Fragments arranged by size:
990 480 161 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 214 740 1701
Size: 214 526 961
Fragments arranged by size:
961 526 214
EaeI y'GGCC_r
Cuts at: 0 521 1126 1701
Size: 521 605 575
Fragments arranged by size:
605 575 521
EarI CTCTTCn'nnn_
Cuts at: 0 77 979 1701
Size: 77 902 722
Fragments arranged by size:
902 722 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 516 676 961 967 1451 1701
Size: 469 47 160 285 6 484 250
Fragments arranged by size:
484 469 285 250 160 47 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 679 1701
Size: 679 1022
EcoO109I rG'GnC_Cy
Cuts at: 0 74 184 822 1075 1701
Size: 74 110 638 253 626
Fragments arranged by size:
638 626 253 110 74
EcoRI G'AATT_C
Cuts at: 0 397 1283 1701
Size: 397 886 418
Fragments arranged by size:
886 418 397
HaeII r_GCGC'y
Cuts at: 0 164 1701
Size: 164 1537
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1123 1155 1701
Size: 1123 32 546
Fragments arranged by size:
1123 546 32
HincII GTy'rAC
Cuts at: 0 315 1116 1701
Size: 315 801 585
Fragments arranged by size:
801 585 315
HindIII A'AGCT_T
Cuts at: 0 1544 1701
Size: 1544 157
KasI G'GCGC_C
Cuts at: 0 160 1701
Size: 160 1541
KpnI G_GTAC'C
Cuts at: 0 1539 1633 1701
Size: 1539 94 68
Fragments arranged by size:
1539 94 68
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1352 1701
Size: 1352 349
MscI TGG'CCA
Cuts at: 0 523 1128 1701
Size: 523 605 573
Fragments arranged by size:
605 573 523
MslI CAynn'nnrTG
Cuts at: 0 1222 1609 1701
Size: 1222 387 92
Fragments arranged by size:
1222 387 92
MspA1I CmG'CkG
Cuts at: 0 480 665 1701
Size: 480 185 1036
Fragments arranged by size:
1036 480 185
NarI GG'CG_CC
Cuts at: 0 161 1701
Size: 161 1540
NcoI C'CATG_G
Cuts at: 0 1162 1701
Size: 1162 539
NspI r_CATG'y
Cuts at: 0 1608 1701
Size: 1608 93
PflMI CCAn_nnn'nTGG
Cuts at: 0 719 1701
Size: 719 982
PfoI T'CCnGG_A
Cuts at: 0 278 977 1701
Size: 278 699 724
Fragments arranged by size:
724 699 278
PmlI CAC'GTG
Cuts at: 0 740 1701
Size: 740 961
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 96 128 1048 1080 1701
Size: 96 32 920 32 621
Fragments arranged by size:
920 621 96 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 74 822 1701
Size: 74 748 879
Fragments arranged by size:
879 748 74
PsiI TTA'TAA
Cuts at: 0 731 1701
Size: 731 970
PspOMI G'GGCC_C
Cuts at: 0 1075 1701
Size: 1075 626
PstI C_TGCA'G
Cuts at: 0 1587 1701
Size: 1587 114
PvuI CG_AT'CG
Cuts at: 0 1095 1701
Size: 1095 606
PvuII CAG'CTG
Cuts at: 0 480 1701
Size: 480 1221
SacII CC_GC'GG
Cuts at: 0 666 1701
Size: 666 1035
SanDI GG'GwC_CC
Cuts at: 0 74 1701
Size: 74 1627
SapI GCTCTTCn'nnn_
Cuts at: 0 979 1701
Size: 979 722
SfcI C'TryA_G
Cuts at: 0 402 847 1583 1701
Size: 402 445 736 118
Fragments arranged by size:
736 445 402 118
SfoI GGC'GCC
Cuts at: 0 162 1701
Size: 162 1539
SspI AAT'ATT
Cuts at: 0 1477 1701
Size: 1477 224
StuI AGG'CCT
Cuts at: 0 88 562 944 1701
Size: 88 474 382 757
Fragments arranged by size:
757 474 382 88
StyI C'CwwG_G
Cuts at: 0 818 1162 1701
Size: 818 344 539
Fragments arranged by size:
818 539 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 491 1701
Size: 491 1210
TatI w'GTAC_w
Cuts at: 0 677 1258 1701
Size: 677 581 443
Fragments arranged by size:
677 581 443
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 467 499 1701
Size: 467 32 1202
Fragments arranged by size:
1202 467 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1224 1701
Size: 1224 477
ZraI GAC'GTC
Cuts at: 0 201 1701
Size: 201 1500
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AgeI AloI ApaI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BciVI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstEII
BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII HindIII KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NspI PflMI PfoI PmlI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII SacII SanDI SapI
SfcI SfoI SspI StuI StyI TaqII TatI TstI
XcmI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AhdI AleI AlfI AlwNI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BclI BfrBI BglI BlpI BmtI BplI BpuEI BsaBI
BsiHKAI BsiWI BspEI BsrBI BssHII BssSI BstAPI BstBI
BstXI BtgZI DraI DrdI EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HpaI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PshAI PspXI PsrI RsrII SacI SalI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI
XmaI XmnI