################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AluI 230 AGCT AG^CT AocI 244 CCTGAGG CC^T[ACGT]AGG AspHI 229 GAGCTC G[AT]GC[AT]^C BanII 229 GAGCTC G[GA]GC[CT]^C BbvCI 96 CCTCAGC CCTCAGC(-5/-2) BbvI 161 GCTGC GCAGC(8/12) BglII 128 AGATCT A^GATCT BmyI 229 GAGCTC G[AGT]GC[ACT]^C BsaJI 80 CCATGG C^C[ACGT][ACGT]GG BsaWI 233 TCCGGT [AT]^CCGG[AT] BsiHKAI 229 GAGCTC G[AT]GC[AT]^C BsmAI 289 GAGAC GTCTC(1/5) Bsp1286I 229 GAGCTC G[AGT]GC[ACT]^C BsrBI 222 GAGCGG CCGCTC(-3/-3) BstXI 188 CCATAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 128 AGATCT [GA]^GATC[CT] Bsu36I 244 CCTGAGG CC^T[ACGT]AGG DsaI 80 CCATGG C^C[GA][CT]GG EaeI 44 CGGCCA [CT]^GGCC[GA] EarI 286 GAAGAG CTCTTC(1/4) Eco81I 244 CCTGAGG CC^T[ACGT]AGG EcoNI 255 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 80 CCATGG C^C[AT][AT]GG HapII 234 CCGG C^CGG HpaII 234 CCGG C^CGG HphI 60 GGTGA GGTGA(8/7) Ksp632I 286 GAAGAG CTCTTC(1/4) MflI 128 AGATCT [GA]^GATC[CT] MslI 189 CATAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 159 CCGCTG C[AC]G^C[GT]G MspI 234 CCGG C^CGG NcoI 80 CCATGG C^CATGG NlaIII 81 CATG CATG^ PflMI 75 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 229 GAGCTC GAGCT^C StyI 80 CCATGG C^C[AT][AT]GG TseI 161 GCTGC G^C[AT]GC TspRI 22 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 75 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 128 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaII 174 GGACC G^G[AT]CC 258 GGTCC BseRI 85 GAGGAG GAGGAG(10/8) 247 GAGGAG BssKI 255 CCTGG ^CC[ACGT]GG 261 CCAGG BstF5I 111 GGATG GGATG(2/0) 267 GGATG BstNI 255 CCTGG CC^[AT]GG 261 CCAGG EcoRII 255 CCTGG ^CC[AT]GG 261 CCAGG FauI 104 CCCGC CCCGC(4/6) 224 GCGGG Fnu4HI 158 GCCGC GC^[ACGT]GC 161 GCTGC FokI 111 GGATG GGATG(9/13) 267 GGATG ItaI 158 GCCGC GC^[ACGT]GC 161 GCTGC MboII 286 GAAGA GAAGA(8/7) 131 TCTTC MvaI 255 CCTGG CC^[AT]GG 261 CCAGG MwoI 107 GCAAGGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 231 GCTCCGGTGGC NlaIV 198 GGAACC GG[ACGT]^[ACGT]CC 239 GGCCCC ScrFI 255 CCTGG CC^[ACGT]GG 261 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AciI BsiYI BslI Cfr13I DdeI DpnII HaeIII MboI MnlI NdeII Sau3AI Sau96I