Restriction map for
pFN21AE0048
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII AccII AccIII AciI AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AviII AvrII BbeI BbrPI
BbsI BbvI BcgI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI Fnu4HI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI ItaI KasI KspI
MamI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI TseI Tth111I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 234 GGTACC G^GTACC
AccI 1080 GTCTAC GT^[AC][GT]AC
AflIII 1409 ACATGT A^C[GA][CT]GT
Asp718I 234 GGTACC G^GTACC
AvaI 1415 CTCGGG C^[CT]CG[GA]G
AvaII 160 GGTCC G^G[AT]CC
BamHI 283 GGATCC G^GATCC
BanII 1026 GAGCCC G[GA]GC[CT]^C
BbvCI 54 CCTCAGC CCTCAGC(-5/-2)
BciVI 621 GTATCC GTATCC(6/5)
BfaI 1070 CTAG C^TAG
BmyI 1026 GAGCCC G[AGT]GC[ACT]^C
BpmI 1385 CTGGAG CTGGAG(16/14)
BsmFI 1174 GTCCC GGGAC(10/14)
BsoBI 1415 CTCGGG C^[CT]CG[GA]G
Bsp1286I 1026 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 612 TGTACA T^GTACA
BspLU11I 1409 ACATGT A^CATGT
BsrGI 612 TGTACA T^GTACA
BstXI 874 CCAGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1140 GCCAGC GC[ACGT]^[ACGT]GC
DraII 894 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 894 AGGCCCT [GA]G^G[ACGT]CC[CT]
HincII 305 GTCAAC GT[CT]^[GA]AC
HindII 305 GTCAAC GT[CT]^[GA]AC
KpnI 234 GGTACC GGTAC^C
MaeI 1070 CTAG C^TAG
NdeI 518 CATATG CA^TATG
NspI 1409 ACATGT [GA]CATG^[CT]
PflMI 1006 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 1555 GATGC GCATC(5/9)
SmlI 297 CTCAAG C^T[CT][GA]AG
SspBI 612 TGTACA T^GTACA
SspI 671 AATATT AAT^ATT
Tsp45I 125 GTGAC ^GT[GC]AC
Van91I 1006 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1069 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 690 AAATTT [GA]^AATT[CT]
999 AAATTC
AlwNI 986 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1166 CAGTGTCTG
ApoI 690 AAATTT [GA]^AATT[CT]
999 AAATTC
BanI 234 GGTACC G^G[CT][GA]CC
1402 GGCACC
BclI 387 TGATCA T^GATCA
549 TGATCA
BcnI 141 CCGGG CC^[GC]GG
287 CCCGG
BseRI 899 CTCCTC GAGGAG(10/8)
1388 GAGGAG
BsmAI 701 GAGAC GTCTC(1/5)
1413 GTCTC
BsrDI 638 CATTGC GCAATG(2/0)
1063 GCAATG
BstYI 283 GGATCC [GA]^GATC[CT]
1479 GGATCT
Cfr13I 160 GGTCC G^G[ACGT]CC
895 GGCCC
DraIII 17 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
905 CACGCAGTG
EcoT14I 60 CCAAGG C^C[AT][AT]GG
1111 CCTTGG
FbaI 387 TGATCA T^GATCA
549 TGATCA
HapII 141 CCGG C^CGG
288 CCGG
HgaI 50 GCGTC GACGC(5/10)
660 GCGTC
HpaII 141 CCGG C^CGG
288 CCGG
HphI 166 GGTGA GGTGA(8/7)
538 GGTGA
MflI 283 GGATCC [GA]^GATC[CT]
1479 GGATCT
MslI 244 CACTTAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1404 CACCCACATG
MspI 141 CCGG C^CGG
288 CCGG
MwoI 50 GCGTCCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1019 GCCCATTGAGC
NciI 141 CCGGG CC^[GC]GG
287 CCCGG
NlaIII 535 CATG CATG^
1410 CATG
Sau96I 160 GGTCC G^G[ACGT]CC
895 GGCCC
StyI 60 CCAAGG C^C[AT][AT]GG
1111 CCTTGG
TfiI 1162 GAATC G^A[AT]TC
1303 GATTC
XhoII 283 GGATCC [GA]^GATC[CT]
1479 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI AluI AlwI BalI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI DdeI DpnII EaeI EarI
EcoRII FokI HaeIII HinfI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MvaI NdeII
NlaIV PleI RsaI Sau3AI ScrFI
TaqI Tru9I Tsp509I TspRI TthHB8I