(Circular) (Six-base) MAPSORT of: pFN21AE0048.seq Check: 3020 from: 1 to: 6271 pFN21AE0048 6271 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4081 278 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 3614 1937 Size: 1149 528 4594 Fragments arranged by size: 4594 1149 528 Acc65I G'GTAC_C Cuts at: 2239 3592 2239 Size: 1353 4918 AclI AA'CG_TT Cuts at: 1563 4398 4771 1563 Size: 2835 373 3063 Fragments arranged by size: 3063 2835 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2337 2668 2843 3195 3204 4334 5718 5986 Size: 705 331 175 352 9 1130 1384 268 Cuts at: 5986 1632 Size: 1917 Fragments arranged by size: 1917 1384 1130 705 352 331 268 175 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6074 AflIII A'CryG_T Cuts at: 1203 3414 5170 6147 1203 Size: 2211 1756 977 1327 Fragments arranged by size: 2211 1756 1327 977 AgeI A'CCGG_T Cuts at: 5102 5102 Size: 6271 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6271 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6271 AlwNI CAG_nnn'CTG Cuts at: 2996 3176 4014 5586 2996 Size: 180 838 1572 3681 Fragments arranged by size: 3681 1572 838 180 ApaLI G'TGCA_C Cuts at: 4328 5484 4328 Size: 1156 5115 ApoI r'AATT_y Cuts at: 1493 2695 3004 3580 3806 3958 6067 1493 Size: 1202 309 576 226 152 2109 1697 Fragments arranged by size: 2109 1697 1202 576 309 226 152 AseI AT'TA_AT Cuts at: 160 1032 4825 160 Size: 872 3793 1606 Fragments arranged by size: 3793 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 3420 3596 1870 Size: 73 1477 176 4545 Fragments arranged by size: 4545 1477 176 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2601 2609 2634 2642 2601 Size: 8 25 8 6230 Fragments arranged by size: 6230 25 8 8 BamHI G'GATC_C Cuts at: 2288 3601 3968 2288 Size: 1313 367 4591 Fragments arranged by size: 4591 1313 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2239 3407 3592 5047 618 Size: 325 217 627 452 1168 185 1455 1842 Fragments arranged by size: 1842 1455 1168 627 452 325 217 185 BanII G_rGCy'C Cuts at: 1391 3035 3590 1391 Size: 1644 555 4072 Fragments arranged by size: 4072 1644 555 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5300 BbvCI CC'TCA_GC Cuts at: 2060 2060 Size: 6271 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4462 4496 1903 Size: 34 2525 34 3678 Fragments arranged by size: 3678 2525 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2637 4163 5373 2637 Size: 1526 1210 3535 Fragments arranged by size: 3535 1526 1210 BclI T'GATC_A Cuts at: 1548 2392 2554 1548 Size: 844 162 5265 Fragments arranged by size: 5265 844 162 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4882 136 Size: 107 122 71 631 605 3210 1525 Fragments arranged by size: 3210 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6266 6266 Size: 6271 BlpI GC'TnA_GC Cuts at: 3688 3688 Size: 6271 Bme1580I G_kGCm'C Cuts at: 1790 4332 5488 1790 Size: 2542 1156 2573 Fragments arranged by size: 2573 2542 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6271 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3146 4002 4960 Size: 416 53 83 399 24 1729 856 958 Cuts at: 4960 442 Size: 1753 Fragments arranged by size: 1753 1729 958 856 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6271 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3411 4931 1239 Size: 2172 1520 2579 Fragments arranged by size: 2579 2172 1520 Bpu10I CC'TnA_GC Cuts at: 2060 2060 Size: 6271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2287 4396 5261 5559 2287 Size: 2109 865 298 2999 Fragments arranged by size: 2999 2109 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4934 882 Size: 617 3435 2219 Fragments arranged by size: 3435 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4062 493 Size: 711 2858 2702 Fragments arranged by size: 2858 2702 711 BsaBI GATnn'nnATC Cuts at: 6265 6265 Size: 6271 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4078 4460 5095 275 Size: 53 83 186 936 2545 382 635 1451 Fragments arranged by size: 2545 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4704 5102 5376 5523 5934 4704 Size: 398 274 147 411 5041 Fragments arranged by size: 5041 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6046 Fragments arranged by size: 6046 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2895 3408 1186 Size: 1709 513 4049 Fragments arranged by size: 4049 1709 513 BseYI C'CCAG_C Cuts at: 5474 5970 5474 Size: 496 5775 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5698 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3721 4482 5510 1540 Size: 275 186 1720 761 1028 2301 Fragments arranged by size: 2301 1720 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4332 4417 5488 4332 Size: 85 1071 5115 Fragments arranged by size: 5115 1071 85 BsmI GAATG_Cn' Cuts at: 3782 3875 3782 Size: 93 6178 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3035 3590 4332 4417 5488 1391 Size: 399 1245 555 742 85 1071 2174 Fragments arranged by size: 2174 1245 1071 742 555 399 85 BspEI T'CCGG_A Cuts at: 5934 5934 Size: 6271 BspHI T'CATG_A Cuts at: 4160 5890 4160 Size: 1730 4541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3626 844 Size: 2782 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4158 1394 Size: 429 2335 3507 Fragments arranged by size: 3507 2335 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2642 3075 4766 4940 65 Size: 1183 1394 433 1691 174 1396 Fragments arranged by size: 1691 1396 1394 1183 433 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4915 5102 1700 Size: 110 3105 187 2869 Fragments arranged by size: 3105 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2617 96 Size: 2521 3750 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6271 BssSI C'ACGA_G Cuts at: 4331 5343 4331 Size: 1012 5259 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6271 BstBI TT'CG_AA Cuts at: 5075 5075 Size: 6271 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6271 BstXI CCAn_nnnn'nTGG Cuts at: 2886 2886 Size: 6271 BstYI r'GATC_y Cuts at: 1972 2288 3484 3601 3968 4353 4370 5822 5908 Size: 316 1196 117 367 385 17 1452 86 Cuts at: 5908 5920 6266 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1196 385 367 346 316 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4780 Fragments arranged by size: 4780 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 2920 3787 4581 4601 2920 Size: 867 794 20 4590 Fragments arranged by size: 4590 867 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6236 DraI TTT'AAA Cuts at: 3575 3575 Size: 6271 DraIII CAC_nnn'GTG Cuts at: 2027 2915 2027 Size: 888 5383 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5278 809 Size: 507 3962 1802 Fragments arranged by size: 3962 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2015 2888 3541 Size: 54 1425 50 275 18 185 873 653 Cuts at: 3541 3718 4607 6236 8 Size: 177 889 1629 43 Fragments arranged by size: 1629 1425 889 873 653 275 185 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3718 1537 Size: 275 1906 4090 Fragments arranged by size: 4090 1906 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2097 2455 2473 3249 4201 1353 Size: 744 358 18 776 952 3423 Fragments arranged by size: 3423 952 776 744 358 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4858 5232 5378 387 Size: 1064 138 3269 374 146 1280 Fragments arranged by size: 3269 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2337 2668 2843 3195 3204 3411 4334 Size: 393 705 331 175 352 9 207 923 Cuts at: 4334 4931 5718 5986 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 923 787 705 597 393 352 331 268 207 175 9 EcoO109I rG'GnC_Cy Cuts at: 2900 2900 Size: 6271 EcoRI G'AATT_C Cuts at: 3580 3958 3580 Size: 378 5893 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2663 3251 2663 Size: 588 5683 FspI TGC'GCA Cuts at: 4777 4777 Size: 6271 HaeII r_GCGC'y Cuts at: 1678 5418 1678 Size: 3740 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2183 2215 2935 2967 3092 Size: 32 135 32 611 32 720 32 125 Cuts at: 3092 3124 3343 3375 4912 4944 4986 5018 1373 Size: 32 219 32 1537 32 42 32 2626 Fragments arranged by size: 2626 1537 720 611 219 135 125 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2312 3615 3861 669 Size: 988 281 374 1303 246 3079 Fragments arranged by size: 3079 1303 988 374 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6271 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KpnI G_GTAC'C Cuts at: 2243 3596 2243 Size: 1353 4918 MfeI C'AATT_G Cuts at: 3870 3870 Size: 6271 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2345 5385 5569 1305 Size: 1040 3040 184 2007 Fragments arranged by size: 3040 2007 1040 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2017 2890 3543 10 Size: 54 1425 343 185 873 653 2738 Fragments arranged by size: 2738 1425 873 653 343 185 54 MslI CAynn'nnrTG Cuts at: 518 1166 2253 3413 4229 4588 4747 518 Size: 648 1087 1160 816 359 159 2042 Fragments arranged by size: 2042 1160 1087 816 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3687 4364 5512 5757 1606 Size: 2081 677 1148 245 2120 Fragments arranged by size: 2120 2081 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4780 Fragments arranged by size: 4780 939 552 NdeI CA'TA_TG Cuts at: 387 2524 387 Size: 2137 4134 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6271 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NspI r_CATG'y Cuts at: 1132 1229 3418 3629 5174 1132 Size: 97 2189 211 1545 2229 Fragments arranged by size: 2229 2189 1545 211 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6271 PciI A'CATG_T Cuts at: 3414 5170 3414 Size: 1756 4515 PflMI CCAn_nnn'nTGG Cuts at: 1108 3017 1108 Size: 1909 4362 PfoI T'CCnGG_A Cuts at: 2291 3388 2291 Size: 1097 5174 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4340 4372 5853 5885 4340 Size: 32 1481 32 4726 Fragments arranged by size: 4726 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6271 PsiI TTA'TAA Cuts at: 3841 3841 Size: 6271 PstI C_TGCA'G Cuts at: 830 3623 830 Size: 2793 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 SalI G'TCGA_C Cuts at: 1936 3613 1936 Size: 1677 4594 SbfI CC_TGCA'GG Cuts at: 3623 3623 Size: 6271 ScaI AGT'ACT Cuts at: 4519 6231 4519 Size: 1712 4559 SfcI C'TryA_G Cuts at: 826 1045 3619 4754 5435 5626 826 Size: 219 2574 1135 681 191 1471 Fragments arranged by size: 2574 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6271 SmaI CCC'GGG Cuts at: 1872 3598 1872 Size: 1726 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2302 4375 5276 5538 820 Size: 197 926 359 2073 901 262 1553 Fragments arranged by size: 2073 1553 926 901 359 262 197 SnaBI TAC'GTA Cuts at: 493 4062 493 Size: 3569 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6271 SphI G_CATG'C Cuts at: 1132 3629 1132 Size: 2497 3774 SspI AAT'ATT Cuts at: 5 52 2678 4195 5 Size: 47 2626 1517 2081 Fragments arranged by size: 2626 2081 1517 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6271 StyI C'CwwG_G Cuts at: 513 1065 2004 2065 3116 3710 513 Size: 552 939 61 1051 594 3074 Fragments arranged by size: 3074 1051 939 594 552 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3425 4298 4315 4468 4653 5098 325 Size: 1255 1845 873 17 153 185 445 1498 Fragments arranged by size: 1845 1498 1255 873 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2617 4517 6229 Size: 274 80 33 51 1443 640 1900 1712 Cuts at: 6229 96 Size: 138 Fragments arranged by size: 1900 1712 1443 640 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6231 6263 6231 Size: 32 6239 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6271 XbaI T'CTAG_A Cuts at: 3074 3607 3074 Size: 533 5738 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6271 XmaI C'CCGG_G Cuts at: 1870 3596 1870 Size: 1726 4545 XmnI GAAnn'nnTTC Cuts at: 4400 4400 Size: 6271 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4079 276 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI