Restriction map for
pFN21AE0048
(Linear) (Six-base) MAPSORT of: KIEE0048.seq Check: 9383 from: 1 to: 1566
KIEE0048.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 1081 1566
Size: 1081 485
Acc65I G'GTAC_C
Cuts at: 0 234 1566
Size: 234 1332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 663 838 1190 1199 1566
Size: 332 331 175 352 9 367
Fragments arranged by size:
367 352 332 331 175 9
AflIII A'CryG_T
Cuts at: 0 1409 1566
Size: 1409 157
AlwNI CAG_nnn'CTG
Cuts at: 0 991 1171 1566
Size: 991 180 395
Fragments arranged by size:
991 395 180
ApoI r'AATT_y
Cuts at: 0 690 999 1566
Size: 690 309 567
Fragments arranged by size:
690 567 309
AvaI C'yCGr_G
Cuts at: 0 1415 1566
Size: 1415 151
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 596 604 629 637 1566
Size: 596 8 25 8 929
Fragments arranged by size:
929 596 25 8 8
BamHI G'GATC_C
Cuts at: 0 283 1566
Size: 283 1283
BanI G'GyrC_C
Cuts at: 0 234 1402 1566
Size: 234 1168 164
Fragments arranged by size:
1168 234 164
BanII G_rGCy'C
Cuts at: 0 1030 1566
Size: 1030 536
BbvCI CC'TCA_GC
Cuts at: 0 55 1566
Size: 55 1511
BciVI GTATCCnnnnn_n'
Cuts at: 0 632 1566
Size: 632 934
BclI T'GATC_A
Cuts at: 0 387 549 1566
Size: 387 162 1017
Fragments arranged by size:
1017 387 162
BmrI ACTGGGnnnn_n'
Cuts at: 0 1141 1566
Size: 1141 425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1406 1566
Size: 1406 160
Bpu10I CC'TnA_GC
Cuts at: 0 55 1566
Size: 55 1511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 1566
Size: 282 1284
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 890 1403 1566
Size: 890 513 163
Fragments arranged by size:
890 513 163
Bsp1286I G_dGCh'C
Cuts at: 0 1030 1566
Size: 1030 536
BsrDI GCAATG_nn'
Cuts at: 0 637 1070 1566
Size: 637 433 496
Fragments arranged by size:
637 496 433
BsrGI T'GTAC_A
Cuts at: 0 612 1566
Size: 612 954
BstXI CCAn_nnnn'nTGG
Cuts at: 0 881 1566
Size: 881 685
BstYI r'GATC_y
Cuts at: 0 283 1479 1566
Size: 283 1196 87
Fragments arranged by size:
1196 283 87
BtsI GCAGTG_nn'
Cuts at: 0 915 1566
Size: 915 651
DraIII CAC_nnn'GTG
Cuts at: 0 22 910 1566
Size: 22 888 656
Fragments arranged by size:
888 656 22
EaeI y'GGCC_r
Cuts at: 0 10 883 1536 1566
Size: 10 873 653 30
Fragments arranged by size:
873 653 30 10
EarI CTCTTCn'nnn_
Cuts at: 0 92 450 468 1244 1566
Size: 92 358 18 776 322
Fragments arranged by size:
776 358 322 92 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 663 838 1190 1199 1406 1566
Size: 332 331 175 352 9 207 160
Fragments arranged by size:
352 332 331 207 175 160 9
EcoO109I rG'GnC_Cy
Cuts at: 0 895 1566
Size: 895 671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 658 1246 1566
Size: 658 588 320
Fragments arranged by size:
658 588 320
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 930 962 1087 1119 1338 1370
Size: 178 32 720 32 125 32 219 32
Cuts at: 1370 1566
Size: 196
Fragments arranged by size:
720 219 196 178 125 32 32 32
32
HincII GTy'rAC
Cuts at: 0 307 1566
Size: 307 1259
KpnI G_GTAC'C
Cuts at: 0 238 1566
Size: 238 1328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1566
Size: 340 1226
MscI TGG'CCA
Cuts at: 0 12 885 1538 1566
Size: 12 873 653 28
Fragments arranged by size:
873 653 28 12
MslI CAynn'nnrTG
Cuts at: 0 248 1408 1566
Size: 248 1160 158
Fragments arranged by size:
1160 248 158
NdeI CA'TA_TG
Cuts at: 0 519 1566
Size: 519 1047
NspI r_CATG'y
Cuts at: 0 1413 1566
Size: 1413 153
PciI A'CATG_T
Cuts at: 0 1409 1566
Size: 1409 157
PflMI CCAn_nnn'nTGG
Cuts at: 0 1012 1566
Size: 1012 554
PfoI T'CCnGG_A
Cuts at: 0 286 1383 1566
Size: 286 1097 183
Fragments arranged by size:
1097 286 183
SmlI C'TyrA_G
Cuts at: 0 297 1566
Size: 297 1269
SspI AAT'ATT
Cuts at: 0 673 1566
Size: 673 893
StyI C'CwwG_G
Cuts at: 0 60 1111 1566
Size: 60 1051 455
Fragments arranged by size:
1051 455 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1420 1566
Size: 1420 146
TatI w'GTAC_w
Cuts at: 0 612 1566
Size: 612 954
XbaI T'CTAG_A
Cuts at: 0 1069 1566
Size: 1069 497
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI ApoI AvaI BaeI
BamHI BanI BanII BbvCI BciVI BclI BmrI BpmI
Bpu10I BpuEI BseRI Bsp1286I BsrDI BsrGI BstXI BstYI
BtsI DraIII EaeI EarI Eco57MI EcoO109I FalI Hin4I
HincII KpnI MmeI MscI MslI NdeI NspI PciI
PflMI PfoI SmlI SspI StyI TaqII TatI XbaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BbeI BbsI BcgI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI MfeI
MluI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI