Restriction map for pFN21AE0230
 (Circular) (Six-base) MAPSORT of: pFN21AE0230.seq  Check: 3147  from: 1  to: 5236



pFN21AE0230  5236 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:46  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3046     278 
   Size:         53      83     186     936    1510    2468

  Fragments arranged by size: 

                2468    1510     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2579    1937 
   Size:        642    4594
 
Acc65I G'GTAC_C

Cuts at:   2557    2557 
   Size:       5236
 
AclI AA'CG_TT

Cuts at:   1563    3363    3736    1563 
   Size:       1800     373    3063

  Fragments arranged by size: 

                3063    1800     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3299    4683    4951    1632 
   Size:       1667    1384     268    1917

  Fragments arranged by size: 

                1917    1667    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5039
 
AflIII A'CryG_T

Cuts at:   1203    4135    5112    1203 
   Size:       2932     977    1327

  Fragments arranged by size: 

                2932    1327     977
 
AgeI A'CCGG_T

Cuts at:   4067    4067 
   Size:       5236
 
AhdI GACnn_n'nnGTC

Cuts at:   3965    3965 
   Size:       5236
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5236
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5236
 
AlwNI CAG_nnn'CTG

Cuts at:   2031    2415    2445    2475    2979    4551    2031 
   Size:        384      30      30     504    1572    2716

  Fragments arranged by size: 

                2716    1572     504     384      30      30
 
ApaLI G'TGCA_C

Cuts at:   3293    4449    3293 
   Size:       1156    4080
 
ApoI r'AATT_y

Cuts at:   1493    2545    2771    2923    5032    1493 
   Size:       1052     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1052     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3790     160 
   Size:        872    2758    1606

  Fragments arranged by size: 

                2758    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5236
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2561    1870 
   Size:         73     618    4545

  Fragments arranged by size: 

                4545     618      73
 
BamHI G'GATC_C

Cuts at:   2566    2933    2566 
   Size:        367    4869
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2212    2557    4012     618 
   Size:        325     217     627     425     345    1455    1842

  Fragments arranged by size: 

                1842    1455     627     425     345     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2255    2285    2555    1391 
   Size:        864      30     270    4072

  Fragments arranged by size: 

                4072     864     270      30
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4265
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3427    3461    1903 
   Size:         34    1490      34    3678

  Fragments arranged by size: 

                3678    1490      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3128    4338    3128 
   Size:       1210    4026
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5236
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2112    3847     136 
   Size:        107     122      71     631     605     440    1735    1525

  Fragments arranged by size: 

                1735    1525     631     605     440     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5231    5231 
   Size:       5236
 
BlpI GC'TnA_GC

Cuts at:   2653    2653 
   Size:       5236
 
Bme1580I G_kGCm'C

Cuts at:   1790    3297    4453    1790 
   Size:       1507    1156    2573

  Fragments arranged by size: 

                2573    1507    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5236
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2341    2967    3925 
   Size:        416      53      83     399      24     924     626     958

Cuts at:   3925     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     924     626     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5236
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2008    2044    2128    2149    3896    1239 
   Size:        769      36      84      21    1747    2579

  Fragments arranged by size: 

                2579    1747     769      84      36      21
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3361    4226    4524    3361 
   Size:        865     298    4073

  Fragments arranged by size: 

                4073     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3899     882 
   Size:        617    2400    2219

  Fragments arranged by size: 

                2400    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3027     493 
   Size:        711    1823    2702

  Fragments arranged by size: 

                2702    1823     711
 
BsaBI GATnn'nnATC

Cuts at:   5230    5230 
   Size:       5236
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3043    3425    4060     275 
   Size:         53      83     186     936    1510     382     635    1451

  Fragments arranged by size: 

                1510    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3669    4067    4341    4488    4899    3669 
   Size:        398     274     147     411    4006

  Fragments arranged by size: 

                4006     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5011

  Fragments arranged by size: 

                5011     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2368    1186 
   Size:       1182    4054
 
BseYI C'CCAG_C

Cuts at:   4439    4935    4439 
   Size:        496    4740
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2212    1163 
   Size:        573     476    4187

  Fragments arranged by size: 

                4187     573     476
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2107    2686    3447    4475    1540 
   Size:        275     186     106     579     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     579     275     186     106
 
BsiHKAI G_wGCw'C

Cuts at:   2207    3297    3382    4453    2207 
   Size:       1090      85    1071    2990

  Fragments arranged by size: 

                2990    1090    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2747    2840    2747 
   Size:         93    5143
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2207    2255    2285    2555    3297    3382    4453 
   Size:        399     417      48      30     270     742      85    1071

Cuts at:   4453    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     417     399     270      85      48
                  30
 
BspEI T'CCGG_A

Cuts at:   4899    4899 
   Size:       5236
 
BspHI T'CATG_A

Cuts at:   3125    4855    3125 
   Size:       1730    3506
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2591     844 
   Size:       1747    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3123    1394 
   Size:        429    1300    3507

  Fragments arranged by size: 

                3507    1300     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3731    3905      65 
   Size:       1183    2483     174    1396

  Fragments arranged by size: 

                2483    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3880    4067    1700 
   Size:        110    2070     187    2869

  Fragments arranged by size: 

                2869    2070     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5236
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5236
 
BssSI C'ACGA_G

Cuts at:   3296    4308    3296 
   Size:       1012    4224
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2424     794 
   Size:       1630    3606
 
BstBI TT'CG_AA

Cuts at:   4040    4040 
   Size:       5236
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5236
 
BstYI r'GATC_y

Cuts at:   1972    2566    2933    3318    3335    4787    4873    4885    5231 
   Size:        594     367     385      17    1452      86      12     346

Cuts at:   5231    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     594     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2380    2380 
   Size:       5236
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2107     513 
   Size:        552     939     103    3642

  Fragments arranged by size: 

                3642     939     552     103
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4497

  Fragments arranged by size: 

                4497     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2752    3546    3566    2752 
   Size:        794      20    4422

  Fragments arranged by size: 

                4422     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5236
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5201
 
DraI TTT'AAA

Cuts at:   2540    2540 
   Size:       5236
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4243     809 
   Size:        507    2927    1802

  Fragments arranged by size: 

                2927    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2104    2683    3572 
   Size:         54    1425      50     275      18     274     579     889

Cuts at:   3572    5201       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     579     275     274      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2104    2683    1537 
   Size:        275     292     579    4090

  Fragments arranged by size: 

                4090     579     292     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3166    1353 
   Size:       1813    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3823    4197    4343     387 
   Size:       1064     138    2234     374     146    1280

  Fragments arranged by size: 

                2234    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2008    2044    2128    2149    3299    3896    4683 
   Size:        393     376      36      84      21    1150     597     787

Cuts at:   4683    4951    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1150     787     597     393     376     268      84
                  36      21
 
EcoRI G'AATT_C

Cuts at:   2545    2923    2545 
   Size:        378    4858
 
FspI TGC'GCA

Cuts at:   3742    3742 
   Size:       5236
 
HaeII r_GCGC'y

Cuts at:   1678    4383    1678 
   Size:       2705    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3877    3909    3951    3983    1373 
   Size:         32     135      32    2305      32      42      32    2626

  Fragments arranged by size: 

                2626    2305     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2580    2826     669 
   Size:        988     281     642     246    3079

  Fragments arranged by size: 

                3079     988     642     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5236
 
HpaI GTT'AAC

Cuts at:   2826    2826 
   Size:       5236
 
KpnI G_GTAC'C

Cuts at:   2561    2561 
   Size:       5236
 
MfeI C'AATT_G

Cuts at:   2835    2835 
   Size:       5236
 
MluI A'CGCG_T

Cuts at:   5112    5112 
   Size:       5236
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2523    4350    4534    1305 
   Size:       1218    1827     184    2007

  Fragments arranged by size: 

                2007    1827    1218     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3414

  Fragments arranged by size: 

                3414    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3194    3553    3712     518 
   Size:        648    2028     359     159    2042

  Fragments arranged by size: 

                2042    2028     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2064    2148    2235    2355    2412    2442    2472    2652 
   Size:        458      84      87     120      57      30      30     180

Cuts at:   2652    3329    4477    4722    1606 
   Size:        677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     458     245     180     120      87
                  84      57      30      30
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3745

  Fragments arranged by size: 

                3745     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5236
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5236
 
NotI GC'GGCC_GC

Cuts at:   2683    2683 
   Size:       5236
 
NspI r_CATG'y

Cuts at:   1132    1229    2594    4139    1132 
   Size:         97    1365    1545    2229

  Fragments arranged by size: 

                2229    1545    1365      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5236
 
PciI A'CATG_T

Cuts at:   4135    4135 
   Size:       5236
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5236
 
PmeI GTTT'AAAC

Cuts at:   2540    2540 
   Size:       5236
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3305    3337    4818    4850    3305 
   Size:         32    1481      32    3691

  Fragments arranged by size: 

                3691    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5236
 
PsiI TTA'TAA

Cuts at:   2806    2806 
   Size:       5236
 
PstI C_TGCA'G

Cuts at:    830    2141    2588     830 
   Size:       1311     447    3478

  Fragments arranged by size: 

                3478    1311     447
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5236
 
PvuII CAG'CTG

Cuts at:   2064    2148    2235    2355    2412    2442    2472    2064 
   Size:         84      87     120      57      30      30    4828

  Fragments arranged by size: 

                4828     120      87      84      57      30      30
 
SalI G'TCGA_C

Cuts at:   1936    2578    1936 
   Size:        642    4594
 
SbfI CC_TGCA'GG

Cuts at:   2588    2588 
   Size:       5236
 
ScaI AGT'ACT

Cuts at:   3484    5196    3484 
   Size:       1712    3524
 
SfcI C'TryA_G

Cuts at:    826    1045    2137    2584    3719    4400    4591     826 
   Size:        219    1092     447    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1092     681     447     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5236
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5236
 
SmaI CCC'GGG

Cuts at:   1872    2563    1872 
   Size:        691    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3340    4241    4503     820 
   Size:        197     926    1397     901     262    1553

  Fragments arranged by size: 

                1553    1397     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3027     493 
   Size:       2534    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5236
 
SphI G_CATG'C

Cuts at:   1132    2594    1132 
   Size:       1462    3774
 
SspI AAT'ATT

Cuts at:      5      52    3160       5 
   Size:         47    3108    2081

  Fragments arranged by size: 

                3108    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5236
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2256    2286    2316    2675     513 
   Size:        552     939     252      30      30     359    3074

  Fragments arranged by size: 

                3074     939     552     359     252      30      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2200    3263    3280    3433    3618    4063     325 
   Size:       1255     620    1063      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1063     620     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3482    5194      96 
   Size:        274      80      33      51    1443    1505    1712     138

  Fragments arranged by size: 

                1712    1505    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5196    5228    5196 
   Size:         32    5204
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5236
 
XbaI T'CTAG_A

Cuts at:   2572    2572 
   Size:       5236
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5236
 
XmaI C'CCGG_G

Cuts at:   1870    2561    1870 
   Size:        691    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3365    3365 
   Size:       5236
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3044     276 
   Size:         53      83     186     936    1510    2468

  Fragments arranged by size: 

                2468    1510     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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