Restriction map for
pFN21AA0166
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AhdI Aor51HI ApaI AscI AspEI
AviII AvrII BbeI BcgI BcnI
BglI BlnI BlpI Bpu1102I BsaHI
BsaI BseAI BsiWI BsmBI BspDI
BspEI BsrBI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I CelII
CfoI ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I FseI FspI HaeII
HhaI Hin1I HinP1I KasI KspI
MluI MroI MvnI NarI NciI
NotI NruI PacI PmeI PpuMI
PshAI Psp1406I RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AgeI 673 ACCGGT A^CCGGT
Alw44I 1688 GTGCAC G^TGCAC
AocI 6301 CCTCAGG CC^T[ACGT]AGG
ApaLI 1688 GTGCAC G^TGCAC
AspI 1141 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5660 CTCGAG C^[CT]CG[GA]G
BamHI 4566 GGATCC G^GATCC
BbrPI 927 CACGTG CAC^GTG
BbvCI 3121 GCTGAGG CCTCAGC(-5/-2)
BciVI 6011 GTATCC GTATCC(6/5)
BsiEI 5233 CGATCG CG[GA][CT]^CG
BsoBI 5660 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2273 TGTACA T^GTACA
BsrGI 2273 TGTACA T^GTACA
BssSI 5866 CACGAG CACGAG(-5/-1)
BstXI 4475 CCAAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 6301 CCTCAGG CC^T[ACGT]AGG
DraIII 3853 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 6301 CCTCAGG CC^T[ACGT]AGG
FauI 6560 GCGGG CCCGC(4/6)
HgaI 1369 GACGC GACGC(5/10)
NaeI 4423 GCCGGC GCC^GGC
NgoMIV 4423 GCCGGC G^CCGGC
PaeR7I 5660 CTCGAG C^TCGAG
PflFI 1141 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1927 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 673 ACCGGT A^CCGGT
PmaCI 927 CACGTG CAC^GTG
PmlI 927 CACGTG CAC^GTG
PvuI 5233 CGATCG CGAT^CG
SexAI 5487 ACCTGGT A^CC[AT]GGT
SspBI 2273 TGTACA T^GTACA
Tth111I 1141 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1927 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4710 TCTAGA T^CTAGA
XhoI 5660 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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Asp700I 883 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4688 GAAGAATTTC
BalI 1924 TGGCCA TGG^CCA
4484 TGGCCA
BclI 2084 TGATCA T^GATCA
6105 TGATCA
BsaBI 388 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1988 GATTGGCATC
BsaWI 37 ACCGGA [AT]^CCGG[AT]
673 ACCGGT
BspHI 3527 TCATGA T^CATGA
3869 TCATGA
BspMI 5924 ACCTGC ACCTGC(4/8)
6027 GCAGGT
BsrDI 4856 CATTGC GCAATG(2/0)
3284 GCAATG
BstBI 4869 TTCGAA TT^CGAA
4927 TTCGAA
EcoNI 5079 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5824 CCTAAAGAAGG
EcoRI 5354 GAATTC G^AATTC
5361 GAATTC
FbaI 2084 TGATCA T^GATCA
6105 TGATCA
HpaI 4959 GTTAAC GTT^AAC
5640 GTTAAC
MamI 388 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1988 GATTGGCATC
MluNI 1924 TGGCCA TGG^CCA
4484 TGGCCA
MscI 1924 TGGCCA TGG^CCA
4484 TGGCCA
MslI 2151 CACCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3280 CACTGCAATG
NspV 4869 TTCGAA TT^CGAA
4927 TTCGAA
RcaI 3527 TCATGA T^CATGA
3869 TCATGA
SacI 1550 GAGCTC GAGCT^C
5663 GAGCTC
ScaI 1158 AGTACT AGT^ACT
4204 AGTACT
SfuI 4869 TTCGAA TT^CGAA
4927 TTCGAA
SphI 154 GCATGC GCATG^C
6456 GCATGC
StuI 128 AGGCCT AGG^CCT
2846 AGGCCT
XcmI 3185 CCAAACAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3559 CCATATGAAGAGTGG
XmnI 883 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4688 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AseI AsnI Asp718I AspHI
AvaII BanI BanII BbsI BbvI
BfaI BfrI BglII BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRII EcoRV
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MspA1I
MspI MunI MvaI MwoI NcoI
NdeI NdeII NheI NlaIII NlaIV
NsiI NspI PleI PshBI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII