################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII AccI AccII AccIII AclI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BlnI BlpI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HincII HindII HpaI HpaII KasI Ksp632I KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 162 GGTACC G^GTACC AciI 1586 CCGC CCGC(-3/-1) AcsI 801 GAATTT [GA]^AATT[CT] AflII 619 CTTAAG C^TTAAG AlwNI 925 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 801 GAATTT [GA]^AATT[CT] Asp718I 162 GGTACC G^GTACC AspHI 1384 GAGCTC G[AT]GC[AT]^C AvaII 1574 GGACC G^G[AT]CC BalI 1238 TGGCCA TGG^CCA BanI 162 GGTACC G^G[CT][GA]CC BanII 1384 GAGCTC G[GA]GC[CT]^C BbsI 676 GAAGAC GAAGAC(2/6) BclI 120 TGATCA T^GATCA BfrI 619 CTTAAG C^TTAAG BglII 982 AGATCT A^GATCT BpuAI 676 GAAGAC GAAGAC(2/6) BsaAI 757 TACGTG [CT]AC^GT[GA] BseRI 911 GAGGAG GAGGAG(10/8) BsiHKAI 1384 GAGCTC G[AT]GC[AT]^C BsmBI 33 CGTCTC CGTCTC(1/5) BsmFI 9 GTCCC GGGAC(10/14) BsmI 1757 GCATTC GAATGC(1/-1) BspHI 776 TCATGA T^CATGA BsrDI 1456 CATTGC GCAATG(2/0) BstEII 1805 GGTCACC G^GT[ACGT]ACC BstPI 1805 GGTCACC G^GT[ACGT]ACC BstXI 890 CCACTCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 1574 GGACC G^G[ACGT]CC DraIII 1748 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1238 TGGCCA [CT]^GGCC[GA] EcoO65I 1805 GGTCACC G^GT[ACGT]ACC EcoT14I 12 CCTTGG C^C[AT][AT]GG FbaI 120 TGATCA T^GATCA HindIII 280 AAGCTT A^AGCTT KpnI 162 GGTACC GGTAC^C MaeII 758 ACGT A^CGT MfeI 1580 CAATTG C^AATTG MluNI 1238 TGGCCA TGG^CCA MscI 1238 TGGCCA TGG^CCA MunI 1580 CAATTG C^AATTG NspI 1460 GCATGT [GA]CATG^[CT] PflMI 166 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1437 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 776 TCATGA T^CATGA SacI 1384 GAGCTC GAGCT^C Sau96I 1574 GGACC G^G[ACGT]CC ScaI 556 AGTACT AGT^ACT SexAI 165 ACCAGGT A^CC[AT]GGT SfcI 1439 CTACAG C^T[GA][CT]AG StyI 12 CCTTGG C^C[AT][AT]GG TfiI 1819 GAATC G^A[AT]TC Van91I 166 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1813 TCTAGA T^CTAGA XcmI 1602 CCAAAGTGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 31 GGCGTC G[GA]^CG[CT]C 210 GGCGTC AlwI 1117 GATCC GGATC(4/5) 744 GGATC BsaHI 31 GGCGTC G[GA]^CG[CT]C 210 GGCGTC BsgI 760 GTGCAG GTGCAG(16/14) 1223 GTGCAG BsmAI 34 GTCTC GTCTC(1/5) 445 GTCTC Bsp1407I 1465 TGTACA T^GTACA 1768 TGTACA BspMI 793 ACCTGC ACCTGC(4/8) 1140 GCAGGT BsrGI 1465 TGTACA T^GTACA 1768 TGTACA BstYI 744 GGATCT [GA]^GATC[CT] 982 AGATCT CfoI 1164 GCGC GCG^C 1218 GCGC HaeIII 1239 GGCC GG^CC 1600 GGCC HgaI 32 GCGTC GACGC(5/10) 211 GCGTC HhaI 1164 GCGC GCG^C 1218 GCGC Hin1I 31 GGCGTC G[GA]^CG[CT]C 210 GGCGTC HinP1I 1164 GCGC G^CGC 1218 GCGC MflI 744 GGATCT [GA]^GATC[CT] 982 AGATCT MspA1I 928 CAGCTG C[AC]G^C[GT]G 1226 CAGCTG NheI 385 GCTAGC G^CTAGC 817 GCTAGC PvuII 928 CAGCTG CAG^CTG 1226 CAGCTG SmlI 619 CTTAAG C^T[CT][GA]AG 1179 CTTGAG SspBI 1465 TGTACA T^GTACA 1768 TGTACA SspI 399 AATATT AAT^ATT 715 AATATT XhoII 744 GGATCT [GA]^GATC[CT] 982 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AfaI AluI BbvI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI SfaNI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I