(Circular) (Six-base) MAPSORT of: pFN21AA1354.seq Check: 7644 from: 1 to: 6556 pFN21AA1354 6556 bp With 182 enzymes: SgfI * March 28, 2012 12:10 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4366 278 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3899 1937 Size: 1962 4594 Acc65I G'GTAC_C Cuts at: 2167 3877 2167 Size: 1710 4846 AclI AA'CG_TT Cuts at: 1563 4683 5056 1563 Size: 3120 373 3063 Fragments arranged by size: 3120 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 2354 2558 3496 3816 3828 4619 6003 Size: 701 21 204 938 320 12 791 1384 Cuts at: 6003 6271 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 938 791 701 320 268 204 21 12 AflII C'TTAA_G Cuts at: 820 1017 2624 820 Size: 197 1607 4752 Fragments arranged by size: 4752 1607 197 AflIII A'CryG_T Cuts at: 1203 5455 6432 1203 Size: 4252 977 1327 Fragments arranged by size: 4252 1327 977 AgeI A'CCGG_T Cuts at: 5387 5387 Size: 6556 AhdI GACnn_n'nnGTC Cuts at: 5285 5285 Size: 6556 AleI CACnn'nnGTG Cuts at: 1166 3186 1166 Size: 2020 4536 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3045 1184 Size: 1861 4695 AlwNI CAG_nnn'CTG Cuts at: 2935 4299 5871 2935 Size: 1364 1572 3620 Fragments arranged by size: 3620 1572 1364 ApaLI G'TGCA_C Cuts at: 4613 5769 4613 Size: 1156 5400 ApoI r'AATT_y Cuts at: 1493 2806 3865 4091 4243 6352 1493 Size: 1313 1059 226 152 2109 1697 Fragments arranged by size: 2109 1697 1313 1059 226 152 AseI AT'TA_AT Cuts at: 160 1032 5110 160 Size: 872 4078 1606 Fragments arranged by size: 4078 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 AvaI C'yCGr_G Cuts at: 1870 1943 3881 1870 Size: 73 1938 4545 Fragments arranged by size: 4545 1938 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2151 2184 2151 Size: 33 6523 BamHI G'GATC_C Cuts at: 3886 4253 3886 Size: 367 6189 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2167 3877 5332 618 Size: 325 217 627 380 1710 1455 1842 Fragments arranged by size: 1842 1710 1455 627 380 325 217 BanII G_rGCy'C Cuts at: 1391 3393 3875 1391 Size: 2002 482 4072 Fragments arranged by size: 4072 2002 482 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2688 928 Size: 971 789 4796 Fragments arranged by size: 4796 971 789 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4747 4781 1903 Size: 34 2810 34 3678 Fragments arranged by size: 3678 2810 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4448 5658 4448 Size: 1210 5346 BclI T'GATC_A Cuts at: 1548 2125 1548 Size: 577 5979 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5167 136 Size: 107 122 71 631 605 3495 1525 Fragments arranged by size: 3495 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2987 6551 2987 Size: 3564 2992 BlpI GC'TnA_GC Cuts at: 3973 3973 Size: 6556 Bme1580I G_kGCm'C Cuts at: 1790 2977 3193 4617 5773 1790 Size: 1187 216 1424 1156 2573 Fragments arranged by size: 2573 1424 1187 1156 216 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6556 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2574 4287 5245 Size: 416 53 83 399 24 1157 1713 958 Cuts at: 5245 442 Size: 1753 Fragments arranged by size: 1753 1713 1157 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2394 2826 1056 Size: 1338 432 4786 Fragments arranged by size: 4786 1338 432 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3503 3503 Size: 6556 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5216 1239 Size: 3977 2579 Bpu10I CC'TnA_GC Cuts at: 2672 2672 Size: 6556 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3205 4681 5546 5844 3205 Size: 1476 865 298 3917 Fragments arranged by size: 3917 1476 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5219 882 Size: 617 3720 2219 Fragments arranged by size: 3720 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2764 4347 493 Size: 711 1560 1583 2702 Fragments arranged by size: 2702 1583 1560 711 BsaBI GATnn'nnATC Cuts at: 6550 6550 Size: 6556 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 2216 4363 4745 Size: 53 83 186 936 504 179 2147 382 Cuts at: 4745 5380 275 Size: 635 1451 Fragments arranged by size: 2147 1451 936 635 504 382 186 179 83 53 BsaWI w'CCGG_w Cuts at: 4989 5387 5661 5808 6219 4989 Size: 398 274 147 411 5326 Fragments arranged by size: 5326 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3612 3642 1178 Size: 30 165 30 2209 30 4092 Fragments arranged by size: 4092 2209 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2931 1186 Size: 1745 4811 BseYI C'CCAG_C Cuts at: 5759 6255 5759 Size: 496 6060 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2786 3249 1163 Size: 573 1050 463 4470 Fragments arranged by size: 4470 1050 573 463 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4006 4767 5795 1540 Size: 275 186 2005 761 1028 2301 Fragments arranged by size: 2301 2005 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3393 4617 4702 5773 3393 Size: 1224 85 1071 4176 Fragments arranged by size: 4176 1224 1071 85 BsmI GAATG_Cn' Cuts at: 3762 4067 4160 3762 Size: 305 93 6158 Fragments arranged by size: 6158 305 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2044 2044 Size: 6556 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2977 3193 3393 3875 4617 4702 5773 Size: 399 1187 216 200 482 742 85 1071 Cuts at: 5773 1391 Size: 2174 Fragments arranged by size: 2174 1187 1071 742 482 399 216 200 85 BspEI T'CCGG_A Cuts at: 6219 6219 Size: 6556 BspHI T'CATG_A Cuts at: 2781 4445 6175 2781 Size: 1664 1730 3162 Fragments arranged by size: 3162 1730 1664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2807 3136 3911 844 Size: 1963 329 775 3489 Fragments arranged by size: 3489 1963 775 329 BsrBI CCG'CTC Cuts at: 1394 1823 4443 1394 Size: 429 2620 3507 Fragments arranged by size: 3507 2620 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3460 5051 5225 65 Size: 1183 2212 1591 174 1396 Fragments arranged by size: 2212 1591 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5200 5387 1700 Size: 110 3390 187 2869 Fragments arranged by size: 3390 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3470 3773 96 Size: 3374 303 2879 Fragments arranged by size: 3374 2879 303 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6556 BssSI C'ACGA_G Cuts at: 4616 5628 4616 Size: 1012 5544 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6556 BstBI TT'CG_AA Cuts at: 5360 5360 Size: 6556 BstEII G'GTnAC_C Cuts at: 1184 3810 1184 Size: 2626 3930 BstXI CCAn_nnnn'nTGG Cuts at: 2902 2902 Size: 6556 BstYI r'GATC_y Cuts at: 1972 2749 2987 3886 4253 4638 4655 6107 6193 Size: 777 238 899 367 385 17 1452 86 Cuts at: 6193 6205 6551 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 899 777 385 367 346 238 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6004 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5817 Fragments arranged by size: 5817 683 56 BtsI GCAGTG_nn' Cuts at: 3232 4072 4866 4886 3232 Size: 840 794 20 4902 Fragments arranged by size: 4902 840 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6556 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6521 DraI TTT'AAA Cuts at: 3860 3860 Size: 6556 DraIII CAC_nnn'GTG Cuts at: 3758 3758 Size: 6556 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5563 809 Size: 507 4247 1802 Fragments arranged by size: 4247 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3243 4003 4892 Size: 54 1425 50 275 18 1413 760 889 Cuts at: 4892 6521 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1413 889 760 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4003 1537 Size: 275 2191 4090 Fragments arranged by size: 4090 2191 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4486 1353 Size: 3133 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5143 5517 5663 387 Size: 1064 138 3554 374 146 1280 Fragments arranged by size: 3554 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3391 3391 Size: 6556 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2354 2558 3496 3816 3828 4619 Size: 393 701 21 204 938 320 12 791 Cuts at: 4619 5216 6003 6271 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 938 791 787 701 597 393 320 268 204 21 12 EcoRI G'AATT_C Cuts at: 3865 4243 3865 Size: 378 6178 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2033 2165 2453 2033 Size: 132 288 6136 Fragments arranged by size: 6136 288 132 FspI TGC'GCA Cuts at: 5062 5062 Size: 6556 HaeII r_GCGC'y Cuts at: 1678 5703 1678 Size: 4025 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2172 2204 3612 3644 5197 Size: 32 135 32 600 32 1408 32 1553 Cuts at: 5197 5229 5271 5303 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1553 1408 600 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3900 4146 669 Size: 988 281 1962 246 3079 Fragments arranged by size: 3079 1962 988 281 246 HindIII A'AGCT_T Cuts at: 748 2285 748 Size: 1537 5019 HpaI GTT'AAC Cuts at: 4146 4146 Size: 6556 KpnI G_GTAC'C Cuts at: 2171 3881 2171 Size: 1710 4846 MfeI C'AATT_G Cuts at: 3585 4155 3585 Size: 570 5986 MluI A'CGCG_T Cuts at: 6432 6432 Size: 6556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2455 2658 3457 3598 5670 5854 1305 Size: 1150 203 799 141 2072 184 2007 Fragments arranged by size: 2072 2007 1150 799 203 184 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 3245 10 Size: 54 1425 343 1413 3321 Fragments arranged by size: 3321 1425 1413 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2980 3186 3207 4514 4873 5032 Size: 648 843 971 206 21 1307 359 159 Cuts at: 5032 518 Size: 2042 Fragments arranged by size: 2042 1307 971 843 648 359 206 159 21 MspA1I CmG'CkG Cuts at: 1606 2935 3233 3972 4649 5797 6042 1606 Size: 1329 298 739 677 1148 245 2120 Fragments arranged by size: 2120 1329 1148 739 677 298 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6004 NdeI CA'TA_TG Cuts at: 387 387 Size: 6556 NheI G'CTAG_C Cuts at: 1052 2390 2822 1052 Size: 1338 432 4786 Fragments arranged by size: 4786 1338 432 NotI GC'GGCC_GC Cuts at: 4003 4003 Size: 6556 NspI r_CATG'y Cuts at: 1132 1229 3469 3914 5459 1132 Size: 97 2240 445 1545 2229 Fragments arranged by size: 2240 2229 1545 445 97 PasI CC'CwG_GG Cuts at: 1348 3806 1348 Size: 2458 4098 PciI A'CATG_T Cuts at: 5455 5455 Size: 6556 PflMI CCAn_nnn'nTGG Cuts at: 1108 2177 1108 Size: 1069 5487 PmeI GTTT'AAAC Cuts at: 3860 3860 Size: 6556 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2631 2663 4625 4657 6138 6170 2631 Size: 32 1962 32 1481 32 3017 Fragments arranged by size: 3017 1962 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3446 1613 Size: 1833 4723 PsiI TTA'TAA Cuts at: 4126 4126 Size: 6556 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2779 2811 2779 Size: 32 6524 PstI C_TGCA'G Cuts at: 830 3908 830 Size: 3078 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6556 PvuII CAG'CTG Cuts at: 2935 3233 2935 Size: 298 6258 SacI G_AGCT'C Cuts at: 3393 3393 Size: 6556 SalI G'TCGA_C Cuts at: 1936 3898 1936 Size: 1962 4594 SbfI CC_TGCA'GG Cuts at: 3908 3908 Size: 6556 ScaI AGT'ACT Cuts at: 2563 4804 6516 2563 Size: 2241 1712 2603 Fragments arranged by size: 2603 2241 1712 SexAI A'CCwGG_T Cuts at: 2170 2170 Size: 6556 SfcI C'TryA_G Cuts at: 826 1045 3444 3904 5039 5720 5911 826 Size: 219 2399 460 1135 681 191 1471 Fragments arranged by size: 2399 1471 1135 681 460 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6556 SmaI CCC'GGG Cuts at: 1872 3883 1872 Size: 2011 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2624 3184 4660 5561 5823 820 Size: 197 926 681 560 1476 901 262 1553 Fragments arranged by size: 1553 1476 926 901 681 560 262 197 SnaBI TAC'GTA Cuts at: 493 4347 493 Size: 3854 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6556 SphI G_CATG'C Cuts at: 1132 3914 1132 Size: 2782 3774 SspI AAT'ATT Cuts at: 5 52 2406 2722 4480 5 Size: 47 2354 316 1758 2081 Fragments arranged by size: 2354 2081 1758 316 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6556 StyI C'CwwG_G Cuts at: 513 1065 2017 3995 513 Size: 552 952 1978 3074 Fragments arranged by size: 3074 1978 952 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4583 4600 4753 4938 5383 325 Size: 1255 3003 17 153 185 445 1498 Fragments arranged by size: 3003 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2561 3470 3773 Size: 274 80 33 51 1443 584 909 303 Cuts at: 3773 4802 6514 96 Size: 1029 1712 138 Fragments arranged by size: 1712 1443 1029 909 584 303 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3772 3804 6516 6548 3772 Size: 32 2712 32 3780 Fragments arranged by size: 3780 2712 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6556 XbaI T'CTAG_A Cuts at: 3818 3892 3818 Size: 74 6482 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3614 3614 Size: 6556 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6556 XmaI C'CCGG_G Cuts at: 1870 3881 1870 Size: 2011 4545 XmnI GAAnn'nnTTC Cuts at: 4685 4685 Size: 6556 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4364 276 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI