(Circular) (Six-base) MAPSORT of: pFN21AE0309.seq Check: 186 from: 1 to: 6427 pFN21AE0309 6427 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2829 2891 2829 Size: 62 6365 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2775 3770 1937 Size: 838 995 4594 Fragments arranged by size: 4594 995 838 Acc65I G'GTAC_C Cuts at: 3748 3748 Size: 6427 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3319 4490 5874 6142 1632 Size: 1687 1171 1384 268 1917 Fragments arranged by size: 1917 1687 1384 1171 268 AfeI AGC'GCT Cuts at: 3404 3404 Size: 6427 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 5326 6303 1203 Size: 4123 977 1327 Fragments arranged by size: 4123 1327 977 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 3607 5156 3607 Size: 1549 4878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6427 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6427 AlwNI CAG_nnn'CTG Cuts at: 4170 5742 4170 Size: 1572 4855 ApaLI G'TGCA_C Cuts at: 2042 4484 5640 2042 Size: 2442 1156 2829 Fragments arranged by size: 2829 2442 1156 ApoI r'AATT_y Cuts at: 1493 2147 3736 3962 4114 6223 1493 Size: 654 1589 226 152 2109 1697 Fragments arranged by size: 2109 1697 1589 654 226 152 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 2571 3752 1870 Size: 73 628 1181 4545 Fragments arranged by size: 4545 1181 628 73 BamHI G'GATC_C Cuts at: 3757 4124 3757 Size: 367 6060 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3375 3516 3576 3748 5203 Size: 325 217 627 1588 141 60 172 1455 Cuts at: 5203 618 Size: 1842 Fragments arranged by size: 1842 1588 1455 627 325 217 172 141 60 BanII G_rGCy'C Cuts at: 1391 3257 3746 1391 Size: 1866 489 4072 Fragments arranged by size: 4072 1866 489 BbeI G_GCGC'C Cuts at: 3580 3580 Size: 6427 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2556 928 Size: 971 657 4799 Fragments arranged by size: 4799 971 657 BbvCI CC'TCA_GC Cuts at: 3194 3476 3194 Size: 282 6145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2338 2372 2842 2876 2921 2955 4618 Size: 34 401 34 470 34 45 34 1663 Cuts at: 4618 4652 1903 Size: 34 3678 Fragments arranged by size: 3678 1663 470 401 45 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4319 5529 4319 Size: 1210 5217 BclI T'GATC_A Cuts at: 1548 2280 1548 Size: 732 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2273 2991 3674 Size: 107 122 71 631 605 601 718 683 Cuts at: 3674 5038 136 Size: 1364 1525 Fragments arranged by size: 1525 1364 718 683 631 605 601 122 107 71 BglII A'GATC_T Cuts at: 3156 3330 6422 3156 Size: 174 3092 3161 Fragments arranged by size: 3161 3092 174 BlpI GC'TnA_GC Cuts at: 3053 3844 3053 Size: 791 5636 Bme1580I G_kGCm'C Cuts at: 1790 2046 2158 2269 4488 5644 1790 Size: 256 112 111 2219 1156 2573 Fragments arranged by size: 2573 2219 1156 256 112 111 BmgBI CAC'GTC Cuts at: 1318 2122 2554 1318 Size: 804 432 5191 Fragments arranged by size: 5191 804 432 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2701 4158 5116 Size: 416 53 83 399 24 1284 1457 958 Cuts at: 5116 442 Size: 1753 Fragments arranged by size: 1753 1457 1284 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3229 3229 Size: 6427 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2291 5087 1239 Size: 812 240 2796 2579 Fragments arranged by size: 2796 2579 812 240 Bpu10I CC'TnA_GC Cuts at: 2667 3194 3476 3617 2667 Size: 527 282 141 5477 Fragments arranged by size: 5477 527 282 141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2114 2579 4552 5417 5715 2114 Size: 465 1973 865 298 2826 Fragments arranged by size: 2826 1973 865 465 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3200 3559 5090 882 Size: 617 1701 359 1531 2219 Fragments arranged by size: 2219 1701 1531 617 359 BsaAI yAC'GTr Cuts at: 493 1204 4218 493 Size: 711 3014 2702 Fragments arranged by size: 3014 2702 711 BsaBI GATnn'nnATC Cuts at: 6421 6421 Size: 6427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 2379 3577 4234 Size: 53 83 186 936 549 297 1198 657 Cuts at: 4234 4616 5251 275 Size: 382 635 1451 Fragments arranged by size: 1451 1198 936 657 635 549 382 297 186 83 53 BsaWI w'CCGG_w Cuts at: 2160 4860 5258 5532 5679 6090 2160 Size: 2700 398 274 147 411 2497 Fragments arranged by size: 2700 2497 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6202 Fragments arranged by size: 6202 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2105 2612 3707 1186 Size: 853 66 507 1095 3906 Fragments arranged by size: 3906 1095 853 507 66 BseYI C'CCAG_C Cuts at: 2949 3272 5630 6126 2949 Size: 323 2358 496 3250 Fragments arranged by size: 3250 2358 496 323 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2371 2836 1163 Size: 573 635 465 4754 Fragments arranged by size: 4754 635 573 465 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2641 3877 4638 5666 1540 Size: 275 186 640 1236 761 1028 2301 Fragments arranged by size: 2301 1236 1028 761 640 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 3059 3257 4488 4573 5644 2046 Size: 1013 198 1231 85 1071 2829 Fragments arranged by size: 2829 1231 1071 1013 198 85 BsmI GAATG_Cn' Cuts at: 3938 4031 3938 Size: 93 6334 BsmBI CGTCTCn'nnnn_ Cuts at: 3215 3215 Size: 6427 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2158 2269 3059 3257 3746 4488 Size: 399 256 112 111 790 198 489 742 Cuts at: 4488 4573 5644 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 790 742 489 399 256 198 112 111 85 BspEI T'CCGG_A Cuts at: 6090 6090 Size: 6427 BspHI T'CATG_A Cuts at: 4316 6046 4316 Size: 1730 4697 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2593 2670 2829 2891 3782 844 Size: 1749 77 159 62 891 3489 Fragments arranged by size: 3489 1749 891 159 77 62 BsrBI CCG'CTC Cuts at: 1394 1823 4314 1394 Size: 429 2491 3507 Fragments arranged by size: 3507 2491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2333 2937 3480 4922 5096 65 Size: 1183 1085 604 543 1442 174 1396 Fragments arranged by size: 1442 1396 1183 1085 604 543 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2811 3408 5071 5258 1700 Size: 110 1001 597 1663 187 2869 Fragments arranged by size: 2869 1663 1001 597 187 110 BsrGI T'GTAC_A Cuts at: 96 3492 96 Size: 3396 3031 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 4487 5499 4487 Size: 1012 5415 BstAPI GCAn_nnn'nTGC Cuts at: 794 2115 794 Size: 1321 5106 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 3241 1184 Size: 2057 4370 BstYI r'GATC_y Cuts at: 1972 2035 2094 3156 3330 3757 4124 4509 4526 Size: 63 59 1062 174 427 367 385 17 Cuts at: 4526 5978 6064 6076 6422 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1062 427 385 367 346 174 86 63 59 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3129 3201 3672 513 Size: 552 939 1125 72 471 3268 Fragments arranged by size: 3268 1125 939 552 471 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2845 487 Size: 683 56 1619 4069 Fragments arranged by size: 4069 1619 683 56 BtsI GCAGTG_nn' Cuts at: 3271 3346 3943 4737 4757 3271 Size: 75 597 794 20 4941 Fragments arranged by size: 4941 794 597 75 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6392 DraI TTT'AAA Cuts at: 3731 3731 Size: 6427 DraIII CAC_nnn'GTG Cuts at: 2506 2506 Size: 6427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5434 809 Size: 507 4118 1802 Fragments arranged by size: 4118 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2840 3040 3313 Size: 54 1425 50 275 18 1010 200 273 Cuts at: 3313 3564 3874 4763 6392 8 Size: 251 310 889 1629 43 Fragments arranged by size: 1629 1425 1010 889 310 275 273 251 200 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2520 3193 4357 1353 Size: 1167 673 1164 3423 Fragments arranged by size: 3423 1167 1164 673 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2675 5014 5388 5534 387 Size: 1064 138 1086 2339 374 146 1280 Fragments arranged by size: 2339 1280 1086 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3255 3255 Size: 6427 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2291 3319 4490 5087 5874 6142 Size: 393 419 240 1028 1171 597 787 268 Cuts at: 6142 1239 Size: 1524 Fragments arranged by size: 1524 1171 1028 787 597 419 393 268 240 EcoO109I rG'GnC_Cy Cuts at: 2654 2767 2887 2654 Size: 113 120 6194 Fragments arranged by size: 6194 120 113 EcoRI G'AATT_C Cuts at: 3736 4114 3736 Size: 378 6049 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2573 2573 Size: 6427 FspI TGC'GCA Cuts at: 4933 4933 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 2812 3406 3580 5574 1678 Size: 1134 594 174 1994 2531 Fragments arranged by size: 2531 1994 1134 594 174 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 2660 2692 2947 Size: 32 135 32 980 32 76 32 255 Cuts at: 2947 2979 3170 3202 3244 3276 3593 3625 5068 Size: 32 191 32 42 32 317 32 1443 Cuts at: 5068 5100 5142 5174 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1443 980 317 255 191 135 76 42 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3771 4017 669 Size: 988 281 1833 246 3079 Fragments arranged by size: 3079 1833 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6427 HpaI GTT'AAC Cuts at: 4017 4017 Size: 6427 KasI G'GCGC_C Cuts at: 3576 3576 Size: 6427 KpnI G_GTAC'C Cuts at: 3752 3752 Size: 6427 MfeI C'AATT_G Cuts at: 2944 4026 2944 Size: 1082 5345 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5541 5725 1305 Size: 4236 184 2007 Fragments arranged by size: 4236 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3566 10 Size: 54 1425 343 1734 2871 Fragments arranged by size: 2871 1734 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4385 4744 4903 518 Size: 648 3219 359 159 2042 Fragments arranged by size: 3219 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2362 3386 3570 3843 4520 5668 5913 1606 Size: 756 1024 184 273 677 1148 245 2120 Fragments arranged by size: 2120 1148 1024 756 677 273 245 184 NaeI GCC'GGC Cuts at: 2813 3410 2813 Size: 597 5830 NarI GG'CG_CC Cuts at: 3577 3577 Size: 6427 NcoI C'CATG_G Cuts at: 513 1065 2004 3129 3672 513 Size: 552 939 1125 543 3268 Fragments arranged by size: 3268 1125 939 552 543 NdeI CA'TA_TG Cuts at: 387 387 Size: 6427 NgoMIV G'CCGG_C Cuts at: 2811 3408 2811 Size: 597 5830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 3394 3785 5330 1132 Size: 97 2165 391 1545 2229 Fragments arranged by size: 2229 2165 1545 391 97 PasI CC'CwG_GG Cuts at: 1348 3427 1348 Size: 2079 4348 PciI A'CATG_T Cuts at: 5326 5326 Size: 6427 PflMI CCAn_nnn'nTGG Cuts at: 1108 2949 1108 Size: 1841 4586 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2654 2767 2654 Size: 113 6314 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6427 PsiI TTA'TAA Cuts at: 3997 3997 Size: 6427 PstI C_TGCA'G Cuts at: 830 2361 2367 3385 3779 830 Size: 1531 6 1018 394 3478 Fragments arranged by size: 3478 1531 1018 394 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 2362 3386 3570 2362 Size: 1024 184 5219 Fragments arranged by size: 5219 1024 184 SacI G_AGCT'C Cuts at: 3257 3257 Size: 6427 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SanDI GG'GwC_CC Cuts at: 2654 2654 Size: 6427 SbfI CC_TGCA'GG Cuts at: 3779 3779 Size: 6427 ScaI AGT'ACT Cuts at: 4675 6387 4675 Size: 1712 4715 SexAI A'CCwGG_T Cuts at: 2739 2769 2739 Size: 30 6397 SfcI C'TryA_G Cuts at: 826 1045 2357 2363 2880 3381 3775 4910 5591 Size: 219 1312 6 517 501 394 1135 681 Cuts at: 5591 5782 826 Size: 191 1471 Fragments arranged by size: 1471 1312 1135 681 517 501 394 219 191 6 SfoI GGC'GCC Cuts at: 3578 3578 Size: 6427 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 3754 1872 Size: 1882 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2129 2558 4531 5432 5694 820 Size: 197 926 186 429 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 429 262 197 186 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3394 3785 1132 Size: 2262 391 3774 Fragments arranged by size: 3774 2262 391 SspI AAT'ATT Cuts at: 5 52 4351 5 Size: 47 4299 2081 Fragments arranged by size: 4299 2081 47 StuI AGG'CCT Cuts at: 1517 3068 1517 Size: 1551 4876 StyI C'CwwG_G Cuts at: 513 1065 2004 2544 3129 3672 3866 513 Size: 552 939 540 585 543 194 3074 Fragments arranged by size: 3074 939 585 552 543 540 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2417 2427 4454 4471 4624 4809 5254 Size: 1255 837 10 2027 17 153 185 445 Cuts at: 5254 325 Size: 1498 Fragments arranged by size: 2027 1498 1255 837 445 185 153 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2725 3492 4673 Size: 274 80 33 51 1443 748 767 1181 Cuts at: 4673 6385 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1181 767 748 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6387 6419 6387 Size: 32 6395 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6427 XbaI T'CTAG_A Cuts at: 3763 3763 Size: 6427 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6427 XmaI C'CCGG_G Cuts at: 1870 3752 1870 Size: 1882 4545 XmnI GAAnn'nnTTC Cuts at: 2406 4556 2406 Size: 2150 4277 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI XcmI