(Circular) (Six-base) MAPSORT of: pFN21AB8289.seq Check: 8076 from: 1 to: 5611 pFN21AB8289 5611 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3421 278 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2954 1937 Size: 1017 4594 Acc65I G'GTAC_C Cuts at: 2932 2932 Size: 5611 AclI AA'CG_TT Cuts at: 1563 3738 4111 1563 Size: 2175 373 3063 Fragments arranged by size: 3063 2175 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2649 3674 5058 5326 1632 Size: 1017 1025 1384 268 1917 Fragments arranged by size: 1917 1384 1025 1017 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5414 AflIII A'CryG_T Cuts at: 1203 2010 4510 5487 1203 Size: 807 2500 977 1327 Fragments arranged by size: 2500 1327 977 807 AgeI A'CCGG_T Cuts at: 4442 4442 Size: 5611 AhdI GACnn_n'nnGTC Cuts at: 4340 4340 Size: 5611 AleI CACnn'nnGTG Cuts at: 1166 2736 2826 1166 Size: 1570 90 3951 Fragments arranged by size: 3951 1570 90 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5611 AlwNI CAG_nnn'CTG Cuts at: 3354 4926 3354 Size: 1572 4039 ApaLI G'TGCA_C Cuts at: 3668 4824 3668 Size: 1156 4455 ApoI r'AATT_y Cuts at: 1493 2920 3146 3298 5407 1493 Size: 1427 226 152 2109 1697 Fragments arranged by size: 2109 1697 1427 226 152 AseI AT'TA_AT Cuts at: 160 1032 4165 160 Size: 872 3133 1606 Fragments arranged by size: 3133 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 AvaI C'yCGr_G Cuts at: 1870 1943 2304 2936 1870 Size: 73 361 632 4545 Fragments arranged by size: 4545 632 361 73 BamHI G'GATC_C Cuts at: 2941 3308 2941 Size: 367 5244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2679 2932 4387 618 Size: 325 217 627 892 253 1455 1842 Fragments arranged by size: 1842 1455 892 627 325 253 217 BanII G_rGCy'C Cuts at: 1391 2145 2282 2436 2802 2930 1391 Size: 754 137 154 366 128 4072 Fragments arranged by size: 4072 754 366 154 137 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4640 BbvCI CC'TCA_GC Cuts at: 2333 2333 Size: 5611 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3802 3836 1903 Size: 34 1865 34 3678 Fragments arranged by size: 3678 1865 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3503 4713 3503 Size: 1210 4401 BclI T'GATC_A Cuts at: 1548 1548 Size: 5611 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2513 2570 4222 Size: 107 122 71 631 605 841 57 1652 Cuts at: 4222 136 Size: 1525 Fragments arranged by size: 1652 1525 841 631 605 122 107 71 57 BglII A'GATC_T Cuts at: 5606 5606 Size: 5611 BlpI GC'TnA_GC Cuts at: 2435 3028 2435 Size: 593 5018 Bme1580I G_kGCm'C Cuts at: 1790 3672 4828 1790 Size: 1882 1156 2573 Fragments arranged by size: 2573 1882 1156 BmgBI CAC'GTC Cuts at: 1318 2011 1318 Size: 693 4918 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3342 4300 442 Size: 416 53 83 399 24 1925 958 1753 Fragments arranged by size: 1925 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5611 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2296 2296 Size: 5611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2324 4271 1239 Size: 869 216 1947 2579 Fragments arranged by size: 2579 1947 869 216 Bpu10I CC'TnA_GC Cuts at: 2333 2430 2333 Size: 97 5514 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3736 4601 4899 3736 Size: 865 298 4448 Fragments arranged by size: 4448 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2087 2153 2318 4274 882 Size: 617 588 66 165 1956 2219 Fragments arranged by size: 2219 1956 617 588 165 66 BsaAI yAC'GTr Cuts at: 493 1204 3402 493 Size: 711 2198 2702 Fragments arranged by size: 2702 2198 711 BsaBI GATnn'nnATC Cuts at: 5605 5605 Size: 5611 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3418 3800 4435 275 Size: 53 83 186 936 1885 382 635 1451 Fragments arranged by size: 1885 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4044 4442 4716 4863 5274 4044 Size: 398 274 147 411 4381 Fragments arranged by size: 4381 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5386 Fragments arranged by size: 5386 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 1186 Size: 919 4692 BseYI C'CCAG_C Cuts at: 2388 4814 5310 2388 Size: 2426 496 2689 Fragments arranged by size: 2689 2426 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5038 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3061 3822 4850 1540 Size: 275 186 1060 761 1028 2301 Fragments arranged by size: 2301 1060 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2282 2436 2759 2802 3672 3757 4828 2282 Size: 154 323 43 870 85 1071 3065 Fragments arranged by size: 3065 1071 870 323 154 85 43 BsmI GAATG_Cn' Cuts at: 3122 3215 3122 Size: 93 5518 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2282 2436 2759 2802 2930 3672 Size: 399 355 137 154 323 43 128 742 Cuts at: 3672 3757 4828 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 355 323 154 137 128 85 43 BspEI T'CCGG_A Cuts at: 5274 5274 Size: 5611 BspHI T'CATG_A Cuts at: 3500 5230 3500 Size: 1730 3881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2336 2966 844 Size: 1492 630 3489 Fragments arranged by size: 3489 1492 630 BsrBI CCG'CTC Cuts at: 1394 1823 3498 1394 Size: 429 1675 3507 Fragments arranged by size: 3507 1675 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4106 4280 65 Size: 1183 2858 174 1396 Fragments arranged by size: 2858 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 2344 4255 4442 1700 Size: 110 250 284 1911 187 2869 Fragments arranged by size: 2869 1911 284 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5611 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5611 BssSI C'ACGA_G Cuts at: 2803 3671 4683 2803 Size: 868 1012 3731 Fragments arranged by size: 3731 1012 868 BstAPI GCAn_nnn'nTGC Cuts at: 794 2555 2853 794 Size: 1761 298 3552 Fragments arranged by size: 3552 1761 298 BstBI TT'CG_AA Cuts at: 4415 4415 Size: 5611 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5611 BstYI r'GATC_y Cuts at: 1972 2151 2941 3308 3693 3710 5162 5248 5260 Size: 179 790 367 385 17 1452 86 12 Cuts at: 5260 5606 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 790 385 367 346 179 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2253 2475 513 Size: 552 939 249 222 3649 Fragments arranged by size: 3649 939 552 249 222 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2808 487 Size: 683 56 1582 3290 Fragments arranged by size: 3290 1582 683 56 BtsI GCAGTG_nn' Cuts at: 2292 2406 2851 3127 3921 3941 2292 Size: 114 445 276 794 20 3962 Fragments arranged by size: 3962 794 445 276 114 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5611 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5576 DraI TTT'AAA Cuts at: 2915 2915 Size: 5611 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4618 809 Size: 507 3302 1802 Fragments arranged by size: 3302 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 2346 2505 Size: 54 1425 50 275 18 432 84 159 Cuts at: 2505 3058 3947 5576 8 Size: 553 889 1629 43 Fragments arranged by size: 1629 1425 889 553 432 275 159 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3058 1537 Size: 275 1246 4090 Fragments arranged by size: 4090 1246 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2270 2359 2645 2894 3541 1353 Size: 917 89 286 249 647 3423 Fragments arranged by size: 3423 917 647 286 249 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4198 4572 4718 387 Size: 1064 138 2609 374 146 1280 Fragments arranged by size: 2609 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2280 2434 2800 2280 Size: 154 366 5091 Fragments arranged by size: 5091 366 154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2324 2649 3674 4271 5058 5326 Size: 393 476 216 325 1025 597 787 268 Cuts at: 5326 1239 Size: 1524 Fragments arranged by size: 1524 1025 787 597 476 393 325 268 216 EcoRI G'AATT_C Cuts at: 2920 3298 2920 Size: 378 5233 FspI TGC'GCA Cuts at: 4117 4117 Size: 5611 HaeII r_GCGC'y Cuts at: 1678 4758 1678 Size: 3080 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4252 4284 4326 4358 1373 Size: 32 135 32 2680 32 42 32 2626 Fragments arranged by size: 2680 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2380 2955 3201 669 Size: 988 281 442 575 246 3079 Fragments arranged by size: 3079 988 575 442 281 246 HindIII A'AGCT_T Cuts at: 748 2221 748 Size: 1473 4138 HpaI GTT'AAC Cuts at: 3201 3201 Size: 5611 KpnI G_GTAC'C Cuts at: 2936 2936 Size: 5611 MfeI C'AATT_G Cuts at: 3210 3210 Size: 5611 MluI A'CGCG_T Cuts at: 5487 5487 Size: 5611 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4725 4909 1305 Size: 3420 184 2007 Fragments arranged by size: 3420 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2507 10 Size: 54 1425 343 675 3114 Fragments arranged by size: 3114 1425 675 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2736 2826 3569 3928 4087 518 Size: 648 843 727 90 743 359 159 2042 Fragments arranged by size: 2042 843 743 727 648 359 159 90 MspA1I CmG'CkG Cuts at: 1606 2555 2664 3027 3704 4852 5097 1606 Size: 949 109 363 677 1148 245 2120 Fragments arranged by size: 2120 1148 949 677 363 245 109 NaeI GCC'GGC Cuts at: 2346 2346 Size: 5611 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4120 Fragments arranged by size: 4120 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5611 NgoMIV G'CCGG_C Cuts at: 2344 2344 Size: 5611 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5611 NotI GC'GGCC_GC Cuts at: 3058 3058 Size: 5611 NspI r_CATG'y Cuts at: 1132 1229 2112 2637 2969 4514 1132 Size: 97 883 525 332 1545 2229 Fragments arranged by size: 2229 1545 883 525 332 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5611 PciI A'CATG_T Cuts at: 4510 4510 Size: 5611 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5611 PfoI T'CCnGG_A Cuts at: 2034 2034 Size: 5611 PmeI GTTT'AAAC Cuts at: 2915 2915 Size: 5611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3680 3712 5193 5225 3680 Size: 32 1481 32 4066 Fragments arranged by size: 4066 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5611 PsiI TTA'TAA Cuts at: 2751 3181 2751 Size: 430 5181 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2115 2147 2115 Size: 32 5579 PstI C_TGCA'G Cuts at: 830 2560 2963 830 Size: 1730 403 3478 Fragments arranged by size: 3478 1730 403 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5611 PvuII CAG'CTG Cuts at: 2555 2555 Size: 5611 SacI G_AGCT'C Cuts at: 2282 2436 2802 2282 Size: 154 366 5091 Fragments arranged by size: 5091 366 154 SalI G'TCGA_C Cuts at: 1936 2953 1936 Size: 1017 4594 SapI GCTCTTCn'nnn_ Cuts at: 2270 2359 2270 Size: 89 5522 SbfI CC_TGCA'GG Cuts at: 2963 2963 Size: 5611 ScaI AGT'ACT Cuts at: 3859 5571 3859 Size: 1712 3899 SfcI C'TryA_G Cuts at: 826 1045 2132 2556 2812 2959 4094 4775 4966 Size: 219 1087 424 256 147 1135 681 191 Cuts at: 4966 826 Size: 1471 Fragments arranged by size: 1471 1135 1087 681 424 256 219 191 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5611 SmaI CCC'GGG Cuts at: 1872 2938 1872 Size: 1066 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3715 4616 4878 820 Size: 197 926 1772 901 262 1553 Fragments arranged by size: 1772 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3402 493 Size: 2909 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5611 SphI G_CATG'C Cuts at: 1132 2969 1132 Size: 1837 3774 SspI AAT'ATT Cuts at: 5 52 3535 5 Size: 47 3483 2081 Fragments arranged by size: 3483 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5611 StyI C'CwwG_G Cuts at: 513 1065 2004 2611 3050 513 Size: 552 939 607 439 3074 Fragments arranged by size: 3074 939 607 552 439 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3638 3655 3808 3993 4438 325 Size: 1255 2058 17 153 185 445 1498 Fragments arranged by size: 2058 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3857 5569 96 Size: 274 80 33 51 1443 1880 1712 138 Fragments arranged by size: 1880 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5571 5603 5571 Size: 32 5579 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5611 XbaI T'CTAG_A Cuts at: 2359 2947 2359 Size: 588 5023 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5611 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5611 XmaI C'CCGG_G Cuts at: 1870 2936 1870 Size: 1066 4545 XmnI GAAnn'nnTTC Cuts at: 3740 3740 Size: 5611 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3419 276 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI