Restriction map for
pFN21AB8289
(Linear) (Six-base) MAPSORT of: KIBB8289.seq Check: 5228 from: 1 to: 906
KIBB8289.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 906
Size: 644 262
AflIII A'CryG_T
Cuts at: 0 5 906
Size: 5 901
AleI CACnn'nnGTG
Cuts at: 0 731 821 906
Size: 731 90 85
Fragments arranged by size:
731 90 85
AvaI C'yCGr_G
Cuts at: 0 299 906
Size: 299 607
BanI G'GyrC_C
Cuts at: 0 674 906
Size: 674 232
BanII G_rGCy'C
Cuts at: 0 140 277 431 797 906
Size: 140 137 154 366 109
Fragments arranged by size:
366 154 140 137 109
BbvCI CC'TCA_GC
Cuts at: 0 328 906
Size: 328 578
BglI GCCn_nnn'nGGC
Cuts at: 0 508 565 906
Size: 508 57 341
Fragments arranged by size:
508 341 57
BlpI GC'TnA_GC
Cuts at: 0 430 906
Size: 430 476
BmgBI CAC'GTC
Cuts at: 0 6 906
Size: 6 900
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 291 906
Size: 291 615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 319 906
Size: 103 216 587
Fragments arranged by size:
587 216 103
Bpu10I CC'TnA_GC
Cuts at: 0 328 425 906
Size: 328 97 481
Fragments arranged by size:
481 328 97
BsaI GGTCTCn'nnnn_
Cuts at: 0 82 148 313 906
Size: 82 66 165 593
Fragments arranged by size:
593 165 82 66
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 906
Size: 100 806
BseYI C'CCAG_C
Cuts at: 0 383 906
Size: 383 523
BsiHKAI G_wGCw'C
Cuts at: 0 277 431 754 797 906
Size: 277 154 323 43 109
Fragments arranged by size:
323 277 154 109 43
Bsp1286I G_dGCh'C
Cuts at: 0 140 277 431 754 797 906
Size: 140 137 154 323 43 109
Fragments arranged by size:
323 154 140 137 109 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 331 906
Size: 331 575
BsrFI r'CCGG_y
Cuts at: 0 55 339 906
Size: 55 284 567
Fragments arranged by size:
567 284 55
BssSI C'ACGA_G
Cuts at: 0 798 906
Size: 798 108
BstAPI GCAn_nnn'nTGC
Cuts at: 0 550 848 906
Size: 550 298 58
Fragments arranged by size:
550 298 58
BstYI r'GATC_y
Cuts at: 0 146 906
Size: 146 760
BtgI C'CryG_G
Cuts at: 0 248 470 906
Size: 248 222 436
Fragments arranged by size:
436 248 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 803 906
Size: 803 103
BtsI GCAGTG_nn'
Cuts at: 0 287 401 846 906
Size: 287 114 445 60
Fragments arranged by size:
445 287 114 60
EaeI y'GGCC_r
Cuts at: 0 257 341 500 906
Size: 257 84 159 406
Fragments arranged by size:
406 257 159 84
EarI CTCTTCn'nnn_
Cuts at: 0 265 354 640 889 906
Size: 265 89 286 249 17
Fragments arranged by size:
286 265 249 89 17
EcoICRI GAG'CTC
Cuts at: 0 275 429 795 906
Size: 275 154 366 111
Fragments arranged by size:
366 275 154 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 319 644 906
Size: 103 216 325 262
Fragments arranged by size:
325 262 216 103
HincII GTy'rAC
Cuts at: 0 375 906
Size: 375 531
HindIII A'AGCT_T
Cuts at: 0 216 906
Size: 216 690
MscI TGG'CCA
Cuts at: 0 502 906
Size: 502 404
MslI CAynn'nnrTG
Cuts at: 0 731 821 906
Size: 731 90 85
Fragments arranged by size:
731 90 85
MspA1I CmG'CkG
Cuts at: 0 550 659 906
Size: 550 109 247
Fragments arranged by size:
550 247 109
NaeI GCC'GGC
Cuts at: 0 341 906
Size: 341 565
NgoMIV G'CCGG_C
Cuts at: 0 339 906
Size: 339 567
NspI r_CATG'y
Cuts at: 0 107 632 906
Size: 107 525 274
Fragments arranged by size:
525 274 107
PfoI T'CCnGG_A
Cuts at: 0 29 906
Size: 29 877
PsiI TTA'TAA
Cuts at: 0 746 906
Size: 746 160
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 110 142 906
Size: 110 32 764
Fragments arranged by size:
764 110 32
PstI C_TGCA'G
Cuts at: 0 555 906
Size: 555 351
PvuII CAG'CTG
Cuts at: 0 550 906
Size: 550 356
SacI G_AGCT'C
Cuts at: 0 277 431 797 906
Size: 277 154 366 109
Fragments arranged by size:
366 277 154 109
SapI GCTCTTCn'nnn_
Cuts at: 0 265 354 906
Size: 265 89 552
Fragments arranged by size:
552 265 89
SfcI C'TryA_G
Cuts at: 0 127 551 807 906
Size: 127 424 256 99
Fragments arranged by size:
424 256 127 99
StyI C'CwwG_G
Cuts at: 0 606 906
Size: 606 300
XbaI T'CTAG_A
Cuts at: 0 354 906
Size: 354 552
Enzymes that do cut:
AcuI AflIII AleI AvaI BanI BanII BbvCI BglI
BlpI BmgBI BplI BpmI Bpu10I BsaI BseRI BseYI
BsiHKAI Bsp1286I BspMI BsrFI BssSI BstAPI BstYI BtgI
BtgZI BtsI EaeI EarI EcoICRI Eco57MI HincII HindIII
MscI MslI MspA1I NaeI NgoMIV NspI PfoI PsiI
PsrI PstI PvuII SacI SapI SfcI StyI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI
BciVI BclI BfrBI BglII Bme1580I BmrI BmtI BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HpaI KasI
KpnI MfeI MluI MmeI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI