Restriction map for
pFN21AB7618
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AhdI Alw44I AocI Aor51HI
ApaLI ApoI AseI AsnI Asp700I
AspEI AspI AvrII BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI MaeI MaeIII
MamI MfeI MluI MroI MseI
MslI MunI NcoI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 605 GGTACC G^GTACC
AflIII 836 ACACGT A^C[GA][CT]GT
AgeI 501 ACCGGT A^CCGGT
AlwNI 563 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 265 GGCGCGCC GG^CGCGCC
Asp718I 605 GGTACC G^GTACC
AspHI 876 GAGCTC G[AT]GC[AT]^C
AviII 899 TGCGCA TGC^GCA
BalI 2 TGGCCA TGG^CCA
BamHI 759 GGATCC G^GATCC
BcgI 734 GCACTGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 301 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 930 CTGCAC GTGCAG(16/14)
BsiHKAI 876 GAGCTC G[AT]GC[AT]^C
BspMI 277 ACCTGC ACCTGC(4/8)
BsrI 736 ACTGG ACTGG(1/-1)
BstXI 482 CCAAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 550 CGGACCG CG^G[AT]CCG
EaeI 2 TGGCCA [CT]^GGCC[GA]
FspI 899 TGCGCA TGC^GCA
HphI 236 TCACC GGTGA(8/7)
KpnI 605 GGTACC GGTAC^C
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NaeI 523 GCCGGC GCC^GGC
NdeI 344 CATATG CA^TATG
NgoMIV 523 GCCGGC G^CCGGC
PinAI 501 ACCGGT A^CCGGT
PstI 752 CTGCAG CTGCA^G
RsrII 550 CGGACCG CG^G[AT]CCG
SacI 876 GAGCTC GAGCT^C
SapI 651 GCTCTTC GCTCTTC(1/4)
SfaNI 894 GCATC GCATC(5/9)
SfcI 752 CTGCAG C^T[GA][CT]AG
SmlI 82 CTCAAG C^T[CT][GA]AG
StuI 299 AGGCCT AGG^CCT
TfiI 956 GATTC G^A[AT]TC
XcmI 212 CCACCTGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BsaI 13 GAGACC GGTCTC(1/5)
778 GAGACC
BsaWI 501 ACCGGT [AT]^CCGG[AT]
989 ACCGGA
BsrFI 501 ACCGGT [GA]^CCGG[CT]
523 GCCGGC
BstF5I 788 CATCC GGATG(2/0)
72 GGATG
BstYI 225 GGATCT [GA]^GATC[CT]
759 GGATCC
Cfr10I 501 ACCGGT [GA]^CCGG[CT]
523 GCCGGC
EcoT14I 482 CCAAGG C^C[AT][AT]GG
636 CCAAGG
FokI 788 CATCC GGATG(9/13)
72 GGATG
KspI 547 CCGCGG CCGC^GG
657 CCGCGG
MflI 225 GGATCT [GA]^GATC[CT]
759 GGATCC
NlaIII 114 CATG CATG^
1071 CATG
PleI 333 GACTC GAGTC(4/5)
740 GACTC
SacII 547 CCGCGG CCGC^GG
657 CCGCGG
StyI 482 CCAAGG C^C[AT][AT]GG
636 CCAAGG
XhoII 225 GGATCT [GA]^GATC[CT]
759 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BanI
BanII BbeI BbrPI BbvI BcnI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI Ksp632I
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIV PmaCI PmlI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfoI SmaI TseI TspRI XmaCI
XmaI