(Circular) (Six-base) MAPSORT of: pFN21AB7618.seq Check: 8437 from: 1 to: 5791 pFN21AB7618 5791 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3601 278 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3134 1937 Size: 1197 4594 Acc65I G'GTAC_C Cuts at: 2610 3112 2610 Size: 502 5289 AclI AA'CG_TT Cuts at: 1563 3918 4291 1563 Size: 2355 373 3063 Fragments arranged by size: 3063 2355 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2912 3854 5238 5506 1632 Size: 1280 942 1384 268 1917 Fragments arranged by size: 1917 1384 1280 942 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5594 AflIII A'CryG_T Cuts at: 1203 2841 4690 5667 1203 Size: 1638 1849 977 1327 Fragments arranged by size: 1849 1638 1327 977 AgeI A'CCGG_T Cuts at: 2506 4622 2506 Size: 2116 3675 AhdI GACnn_n'nnGTC Cuts at: 4520 4520 Size: 5791 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5791 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5791 AlwNI CAG_nnn'CTG Cuts at: 2573 3534 5106 2573 Size: 961 1572 3258 Fragments arranged by size: 3258 1572 961 ApaI G_GGCC'C Cuts at: 2060 2126 2258 2456 2523 2060 Size: 66 132 198 67 5328 Fragments arranged by size: 5328 198 132 67 66 ApaLI G'TGCA_C Cuts at: 3848 5004 3848 Size: 1156 4635 ApoI r'AATT_y Cuts at: 1493 3100 3326 3478 5587 1493 Size: 1607 226 152 2109 1697 Fragments arranged by size: 2109 1697 1607 226 152 AscI GG'CGCG_CC Cuts at: 2271 2271 Size: 5791 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 2125 2180 2251 2333 2362 2401 2430 Size: 73 182 55 71 82 29 39 29 Cuts at: 2430 2480 2515 2536 2726 2747 2854 3116 1870 Size: 50 35 21 190 21 107 262 4545 Fragments arranged by size: 4545 262 190 182 107 82 73 71 55 50 39 35 29 29 21 21 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2495 2528 2495 Size: 33 5758 BamHI G'GATC_C Cuts at: 2764 3121 3488 2764 Size: 357 367 5067 Fragments arranged by size: 5067 367 357 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2293 2525 2585 2610 2629 Size: 325 217 627 506 232 60 25 19 Cuts at: 2629 3112 4567 618 Size: 483 1455 1842 Fragments arranged by size: 1842 1455 627 506 483 325 232 217 60 25 19 BanII G_rGCy'C Cuts at: 1391 2060 2126 2258 2265 2431 2437 2456 2487 Size: 669 66 132 7 166 6 19 31 Cuts at: 2487 2523 2670 2885 3110 1391 Size: 36 147 215 225 4072 Fragments arranged by size: 4072 669 225 215 166 147 132 66 36 31 19 7 6 BbeI G_GCGC'C Cuts at: 2297 2529 2589 2633 2297 Size: 232 60 44 5455 Fragments arranged by size: 5455 232 60 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4820 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2728 2762 3982 4016 1903 Size: 34 791 34 1220 34 3678 Fragments arranged by size: 3678 1220 791 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3683 4893 3683 Size: 1210 4581 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2312 4402 136 Size: 107 122 71 631 605 640 2090 1525 Fragments arranged by size: 2090 1525 640 631 605 122 107 71 BglII A'GATC_T Cuts at: 5786 5786 Size: 5791 BlpI GC'TnA_GC Cuts at: 3208 3208 Size: 5791 Bme1580I G_kGCm'C Cuts at: 1790 2060 2126 2258 2456 2523 2649 2741 3852 Size: 270 66 132 198 67 126 92 1111 Cuts at: 3852 5008 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1111 270 198 132 126 92 67 66 BmgBI CAC'GTC Cuts at: 1318 2697 1318 Size: 1379 4412 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3522 4480 442 Size: 416 53 83 399 24 2105 958 1753 Fragments arranged by size: 2105 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2416 2416 Size: 5791 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2451 2654 2977 4451 1239 Size: 1212 203 323 1474 2579 Fragments arranged by size: 2579 1474 1212 323 203 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2072 3916 4781 5079 2072 Size: 1844 865 298 2784 Fragments arranged by size: 2784 1844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2012 2777 4454 882 Size: 617 513 765 1677 2219 Fragments arranged by size: 2219 1677 765 617 513 BsaAI yAC'GTr Cuts at: 493 1204 2161 2844 2940 3041 3582 493 Size: 711 957 683 96 101 541 2702 Fragments arranged by size: 2702 957 711 683 541 101 96 BsaBI GATnn'nnATC Cuts at: 5785 5785 Size: 5791 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2214 2294 2442 2526 Size: 53 83 186 936 681 80 148 84 Cuts at: 2526 2579 2586 2630 3598 3980 4615 275 Size: 53 7 44 968 382 635 1451 Fragments arranged by size: 1451 968 936 681 635 382 186 148 84 83 80 53 53 44 7 BsaWI w'CCGG_w Cuts at: 2506 2994 4224 4622 4896 5043 5454 2506 Size: 488 1230 398 274 147 411 2843 Fragments arranged by size: 2843 1230 488 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5566 Fragments arranged by size: 5566 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2225 2228 2524 1186 Size: 1039 3 296 4453 Fragments arranged by size: 4453 1039 296 3 BseYI C'CCAG_C Cuts at: 2297 2375 2455 4994 5490 2297 Size: 78 80 2539 496 2598 Fragments arranged by size: 2598 2539 496 80 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2920 1163 Size: 573 1184 4034 Fragments arranged by size: 4034 1184 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3241 4002 5030 1540 Size: 275 186 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2885 3852 3937 5008 2885 Size: 967 85 1071 3668 Fragments arranged by size: 3668 1071 967 85 BsmI GAATG_Cn' Cuts at: 3302 3395 3302 Size: 93 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2126 2258 2265 2431 2437 2456 Size: 399 270 66 132 7 166 6 19 Cuts at: 2456 2487 2523 2649 2670 2741 2885 3110 3852 Size: 31 36 126 21 71 144 225 742 Cuts at: 3852 3937 5008 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 270 225 166 144 132 126 85 71 66 36 31 21 19 7 6 BspEI T'CCGG_A Cuts at: 5454 5454 Size: 5791 BspHI T'CATG_A Cuts at: 3680 5410 3680 Size: 1730 4061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2291 3146 844 Size: 1447 855 3489 Fragments arranged by size: 3489 1447 855 BsrBI CCG'CTC Cuts at: 1394 1823 3678 1394 Size: 429 1855 3507 Fragments arranged by size: 3507 1855 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4286 4460 65 Size: 1183 3038 174 1396 Fragments arranged by size: 3038 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2506 2528 4435 4622 1700 Size: 110 696 22 1907 187 2869 Fragments arranged by size: 2869 1907 696 187 110 22 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5791 BssHII G'CGCG_C Cuts at: 1925 2271 2819 2847 2966 1925 Size: 346 548 28 119 4750 Fragments arranged by size: 4750 548 346 119 28 BssSI C'ACGA_G Cuts at: 3851 4863 3851 Size: 1012 4779 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5791 BstBI TT'CG_AA Cuts at: 4595 4595 Size: 5791 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5791 BstXI CCAn_nnnn'nTGG Cuts at: 2494 2494 Size: 5791 BstYI r'GATC_y Cuts at: 1972 2230 2764 3121 3488 3873 3890 5342 5428 Size: 258 534 357 367 385 17 1452 86 Cuts at: 5428 5440 5786 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 534 385 367 357 346 258 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2142 2552 2662 513 Size: 552 939 138 410 110 3642 Fragments arranged by size: 3642 939 552 410 138 110 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2633 487 Size: 683 56 1407 3645 Fragments arranged by size: 3645 1407 683 56 BtsI GCAGTG_nn' Cuts at: 3307 4101 4121 3307 Size: 794 20 4977 Fragments arranged by size: 4977 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5756 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4798 809 Size: 507 3482 1802 Fragments arranged by size: 3482 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3238 4127 Size: 54 1425 50 275 18 177 1231 889 Cuts at: 4127 5756 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1231 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3238 1537 Size: 275 1426 4090 Fragments arranged by size: 4090 1426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2100 2184 2663 3721 1353 Size: 747 84 479 1058 3423 Fragments arranged by size: 3423 1058 747 479 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2313 4378 4752 4898 387 Size: 1064 138 724 2065 374 146 1280 Fragments arranged by size: 2065 1280 1064 724 374 146 138 EcoICRI GAG'CTC Cuts at: 2883 2883 Size: 5791 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2451 2654 2912 2977 3854 4451 5238 Size: 393 819 203 258 65 877 597 787 Cuts at: 5238 5506 1239 Size: 268 1524 Fragments arranged by size: 1524 877 819 787 597 393 268 258 203 65 EcoO109I rG'GnC_Cy Cuts at: 2057 2129 2224 2519 2675 2723 2057 Size: 72 95 295 156 48 5125 Fragments arranged by size: 5125 295 156 95 72 48 EcoRI G'AATT_C Cuts at: 3100 3478 3100 Size: 378 5413 FspI TGC'GCA Cuts at: 2906 4297 2906 Size: 1391 4400 HaeII r_GCGC'y Cuts at: 1678 2101 2297 2495 2501 2529 2589 2633 3016 Size: 423 196 198 6 28 60 44 383 Cuts at: 3016 3085 4938 1678 Size: 69 1853 2531 Fragments arranged by size: 2531 1853 423 383 198 196 69 60 44 28 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2272 2304 4432 4464 4506 Size: 32 135 32 700 32 2128 32 42 Cuts at: 4506 4538 1373 Size: 32 2626 Fragments arranged by size: 2626 2128 700 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3135 3381 669 Size: 988 281 1197 246 3079 Fragments arranged by size: 3079 1197 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5791 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KasI G'GCGC_C Cuts at: 2293 2525 2585 2629 2293 Size: 232 60 44 5455 Fragments arranged by size: 5455 232 60 44 KpnI G_GTAC'C Cuts at: 2614 3116 2614 Size: 502 5289 MfeI C'AATT_G Cuts at: 3390 3390 Size: 5791 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4905 5089 1305 Size: 3600 184 2007 Fragments arranged by size: 3600 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3792 Fragments arranged by size: 3792 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3749 4108 4267 518 Size: 648 2583 359 159 2042 Fragments arranged by size: 2583 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2554 2664 3024 3207 3884 5032 5277 1606 Size: 948 110 360 183 677 1148 245 2120 Fragments arranged by size: 2120 1148 948 677 360 245 183 110 NaeI GCC'GGC Cuts at: 2530 2530 Size: 5791 NarI GG'CG_CC Cuts at: 2294 2526 2586 2630 2294 Size: 232 60 44 5455 Fragments arranged by size: 5455 232 60 44 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4300 Fragments arranged by size: 4300 939 552 NdeI CA'TA_TG Cuts at: 387 2350 387 Size: 1963 3828 NgoMIV G'CCGG_C Cuts at: 2528 2528 Size: 5791 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 3149 4694 1132 Size: 97 1920 1545 2229 Fragments arranged by size: 2229 1920 1545 97 PasI CC'CwG_GG Cuts at: 1348 2053 1348 Size: 705 5086 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5791 PfoI T'CCnGG_A Cuts at: 2064 2064 Size: 5791 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PmlI CAC'GTG Cuts at: 2161 2844 2940 3041 2161 Size: 683 96 101 4911 Fragments arranged by size: 4911 683 101 96 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 5373 5405 3860 Size: 32 1481 32 4246 Fragments arranged by size: 4246 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2129 2224 2675 2723 2129 Size: 95 451 48 5197 Fragments arranged by size: 5197 451 95 48 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3361 3361 Size: 5791 PspOMI G'GGCC_C Cuts at: 2056 2122 2254 2452 2519 2056 Size: 66 132 198 67 5328 Fragments arranged by size: 5328 198 132 67 66 PstI C_TGCA'G Cuts at: 830 2761 3143 830 Size: 1931 382 3478 Fragments arranged by size: 3478 1931 382 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 RsrII CG'GwC_CG Cuts at: 2556 2556 Size: 5791 SacI G_AGCT'C Cuts at: 2885 2885 Size: 5791 SacII CC_GC'GG Cuts at: 2555 2665 2555 Size: 110 5681 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SanDI GG'GwC_CC Cuts at: 2675 2723 2675 Size: 48 5743 SapI GCTCTTCn'nnn_ Cuts at: 2663 2663 Size: 5791 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 4039 5751 4039 Size: 1712 4079 SfcI C'TryA_G Cuts at: 826 1045 2757 3139 4274 4955 5146 826 Size: 219 1712 382 1135 681 191 1471 Fragments arranged by size: 1712 1471 1135 681 382 219 191 SfoI GGC'GCC Cuts at: 2295 2527 2587 2631 2295 Size: 232 60 44 5455 Fragments arranged by size: 5455 232 60 44 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5791 SmaI CCC'GGG Cuts at: 1872 2127 2182 2364 2432 2517 2728 2856 3118 Size: 255 55 182 68 85 211 128 262 Cuts at: 3118 1872 Size: 4545 Fragments arranged by size: 4545 262 255 211 182 128 85 68 55 SmlI C'TyrA_G Cuts at: 820 1017 1943 2087 3895 4796 5058 820 Size: 197 926 144 1808 901 262 1553 Fragments arranged by size: 1808 1553 926 901 262 197 144 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5791 SphI G_CATG'C Cuts at: 1132 3149 1132 Size: 2017 3774 SrfI GCCC'GGGC Cuts at: 2432 2432 Size: 5791 SspI AAT'ATT Cuts at: 5 52 3715 5 Size: 47 3663 2081 Fragments arranged by size: 3663 2081 47 StuI AGG'CCT Cuts at: 1517 2306 1517 Size: 789 5002 StyI C'CwwG_G Cuts at: 513 1065 2004 2487 2641 3230 513 Size: 552 939 483 154 589 3074 Fragments arranged by size: 3074 939 589 552 483 154 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2398 3818 3835 3988 4173 4618 325 Size: 1255 818 1420 17 153 185 445 1498 Fragments arranged by size: 1498 1420 1255 818 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4037 5749 96 Size: 274 80 33 51 1443 2060 1712 138 Fragments arranged by size: 2060 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5751 5783 5751 Size: 32 5759 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5791 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2224 2224 Size: 5791 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5791 XmaI C'CCGG_G Cuts at: 1870 2125 2180 2362 2430 2515 2726 2854 3116 Size: 255 55 182 68 85 211 128 262 Cuts at: 3116 1870 Size: 4545 Fragments arranged by size: 4545 262 255 211 182 128 85 68 55 XmnI GAAnn'nnTTC Cuts at: 3920 3920 Size: 5791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3599 276 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI PvuII SexAI SfiI SwaI