Restriction map for
pFN21AB7618
(Linear) (Six-base) MAPSORT of: KIBB7618.seq Check: 3000 from: 1 to: 1086
KIBB7618.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
Acc65I G'GTAC_C
Cuts at: 0 605 1086
Size: 605 481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1086
Size: 907 179
AflIII A'CryG_T
Cuts at: 0 836 1086
Size: 836 250
AgeI A'CCGG_T
Cuts at: 0 501 1086
Size: 501 585
AlwNI CAG_nnn'CTG
Cuts at: 0 568 1086
Size: 568 518
ApaI G_GGCC'C
Cuts at: 0 55 121 253 451 518 1086
Size: 55 66 132 198 67 568
Fragments arranged by size:
568 198 132 67 66 55
AscI GG'CGCG_CC
Cuts at: 0 266 1086
Size: 266 820
AvaI C'yCGr_G
Cuts at: 0 120 175 246 328 357 396 425 475
Size: 120 55 71 82 29 39 29 50
Cuts at: 475 510 531 721 742 849 1086
Size: 35 21 190 21 107 237
Fragments arranged by size:
237 190 120 107 82 71 55 50
39 35 29 29 21 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 490 523 1086
Size: 490 33 563
Fragments arranged by size:
563 490 33
BamHI G'GATC_C
Cuts at: 0 759 1086
Size: 759 327
BanI G'GyrC_C
Cuts at: 0 288 520 580 605 624 1086
Size: 288 232 60 25 19 462
Fragments arranged by size:
462 288 232 60 25 19
BanII G_rGCy'C
Cuts at: 0 55 121 253 260 426 432 451 482
Size: 55 66 132 7 166 6 19 31
Cuts at: 482 518 665 880 1086
Size: 36 147 215 206
Fragments arranged by size:
215 206 166 147 132 66 55 36
31 19 7 6
BbeI G_GCGC'C
Cuts at: 0 292 524 584 628 1086
Size: 292 232 60 44 458
Fragments arranged by size:
458 292 232 60 44
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 723 757 1086
Size: 723 34 329
Fragments arranged by size:
723 329 34
BglI GCCn_nnn'nGGC
Cuts at: 0 307 1086
Size: 307 779
Bme1580I G_kGCm'C
Cuts at: 0 55 121 253 451 518 644 736 1086
Size: 55 66 132 198 67 126 92 350
Fragments arranged by size:
350 198 132 126 92 67 66 55
BmgBI CAC'GTC
Cuts at: 0 692 1086
Size: 692 394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 411 1086
Size: 411 675
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 649 972 1086
Size: 446 203 323 114
Fragments arranged by size:
446 323 203 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1086
Size: 67 1019
BsaI GGTCTCn'nnnn_
Cuts at: 0 7 772 1086
Size: 7 765 314
Fragments arranged by size:
765 314 7
BsaAI yAC'GTr
Cuts at: 0 156 839 935 1036 1086
Size: 156 683 96 101 50
Fragments arranged by size:
683 156 101 96 50
BsaHI Gr'CG_yC
Cuts at: 0 209 289 437 521 574 581 625 1086
Size: 209 80 148 84 53 7 44 461
Fragments arranged by size:
461 209 148 84 80 53 44 7
BsaWI w'CCGG_w
Cuts at: 0 501 989 1086
Size: 501 488 97
Fragments arranged by size:
501 488 97
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 223 519 1086
Size: 220 3 296 567
Fragments arranged by size:
567 296 220 3
BseYI C'CCAG_C
Cuts at: 0 292 370 450 1086
Size: 292 78 80 636
Fragments arranged by size:
636 292 80 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 915 1086
Size: 915 171
BsiHKAI G_wGCw'C
Cuts at: 0 880 1086
Size: 880 206
Bsp1286I G_dGCh'C
Cuts at: 0 55 121 253 260 426 432 451 482
Size: 55 66 132 7 166 6 19 31
Cuts at: 482 518 644 665 736 880 1086
Size: 36 126 21 71 144 206
Fragments arranged by size:
206 166 144 132 126 71 66 55
36 31 21 19 7 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 286 1086
Size: 286 800
BsrFI r'CCGG_y
Cuts at: 0 501 523 1086
Size: 501 22 563
Fragments arranged by size:
563 501 22
BssHII G'CGCG_C
Cuts at: 0 266 814 842 961 1086
Size: 266 548 28 119 125
Fragments arranged by size:
548 266 125 119 28
BstXI CCAn_nnnn'nTGG
Cuts at: 0 489 1086
Size: 489 597
BstYI r'GATC_y
Cuts at: 0 225 759 1086
Size: 225 534 327
Fragments arranged by size:
534 327 225
BtgI C'CryG_G
Cuts at: 0 137 547 657 1086
Size: 137 410 110 429
Fragments arranged by size:
429 410 137 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 628 1086
Size: 628 458
EaeI y'GGCC_r
Cuts at: 0 2 1086
Size: 2 1084
EarI CTCTTCn'nnn_
Cuts at: 0 95 179 658 1086
Size: 95 84 479 428
Fragments arranged by size:
479 428 95 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 308 1086
Size: 308 778
EcoICRI GAG'CTC
Cuts at: 0 878 1086
Size: 878 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 649 907 972 1086
Size: 446 203 258 65 114
Fragments arranged by size:
446 258 203 114 65
EcoO109I rG'GnC_Cy
Cuts at: 0 52 124 219 514 670 718 1086
Size: 52 72 95 295 156 48 368
Fragments arranged by size:
368 295 156 95 72 52 48
FspI TGC'GCA
Cuts at: 0 901 1086
Size: 901 185
HaeII r_GCGC'y
Cuts at: 0 96 292 490 496 524 584 628 1011
Size: 96 196 198 6 28 60 44 383
Cuts at: 1011 1080 1086
Size: 69 6
Fragments arranged by size:
383 198 196 96 69 60 44 28
6 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 267 299 1086
Size: 267 32 787
Fragments arranged by size:
787 267 32
KasI G'GCGC_C
Cuts at: 0 288 520 580 624 1086
Size: 288 232 60 44 462
Fragments arranged by size:
462 288 232 60 44
KpnI G_GTAC'C
Cuts at: 0 609 1086
Size: 609 477
MscI TGG'CCA
Cuts at: 0 4 1086
Size: 4 1082
MspA1I CmG'CkG
Cuts at: 0 549 659 1019 1086
Size: 549 110 360 67
Fragments arranged by size:
549 360 110 67
NaeI GCC'GGC
Cuts at: 0 525 1086
Size: 525 561
NarI GG'CG_CC
Cuts at: 0 289 521 581 625 1086
Size: 289 232 60 44 461
Fragments arranged by size:
461 289 232 60 44
NdeI CA'TA_TG
Cuts at: 0 345 1086
Size: 345 741
NgoMIV G'CCGG_C
Cuts at: 0 523 1086
Size: 523 563
PasI CC'CwG_GG
Cuts at: 0 48 1086
Size: 48 1038
PfoI T'CCnGG_A
Cuts at: 0 59 1086
Size: 59 1027
PmlI CAC'GTG
Cuts at: 0 156 839 935 1036 1086
Size: 156 683 96 101 50
Fragments arranged by size:
683 156 101 96 50
PpuMI rG'GwC_Cy
Cuts at: 0 124 219 670 718 1086
Size: 124 95 451 48 368
Fragments arranged by size:
451 368 124 95 48
PspOMI G'GGCC_C
Cuts at: 0 51 117 249 447 514 1086
Size: 51 66 132 198 67 572
Fragments arranged by size:
572 198 132 67 66 51
PstI C_TGCA'G
Cuts at: 0 756 1086
Size: 756 330
RsrII CG'GwC_CG
Cuts at: 0 551 1086
Size: 551 535
SacI G_AGCT'C
Cuts at: 0 880 1086
Size: 880 206
SacII CC_GC'GG
Cuts at: 0 550 660 1086
Size: 550 110 426
Fragments arranged by size:
550 426 110
SanDI GG'GwC_CC
Cuts at: 0 670 718 1086
Size: 670 48 368
Fragments arranged by size:
670 368 48
SapI GCTCTTCn'nnn_
Cuts at: 0 658 1086
Size: 658 428
SfcI C'TryA_G
Cuts at: 0 752 1086
Size: 752 334
SfoI GGC'GCC
Cuts at: 0 290 522 582 626 1086
Size: 290 232 60 44 460
Fragments arranged by size:
460 290 232 60 44
SmaI CCC'GGG
Cuts at: 0 122 177 359 427 512 723 851 1086
Size: 122 55 182 68 85 211 128 235
Fragments arranged by size:
235 211 182 128 122 85 68 55
SmlI C'TyrA_G
Cuts at: 0 82 1086
Size: 82 1004
SrfI GCCC'GGGC
Cuts at: 0 427 1086
Size: 427 659
StuI AGG'CCT
Cuts at: 0 301 1086
Size: 301 785
StyI C'CwwG_G
Cuts at: 0 482 636 1086
Size: 482 154 450
Fragments arranged by size:
482 450 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 393 1086
Size: 393 693
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 1086
Size: 219 867
XmaI C'CCGG_G
Cuts at: 0 120 175 357 425 510 721 849 1086
Size: 120 55 182 68 85 211 128 237
Fragments arranged by size:
237 211 182 128 120 85 68 55
Enzymes that do cut:
Acc65I AcuI AflIII AgeI AlwNI ApaI AscI AvaI
BaeI BamHI BanI BanII BbeI BcgI BglI Bme1580I
BmgBI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BssHII
BstXI BstYI BtgI BtgZI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FspI HaeII Hin4I KasI KpnI MscI
MspA1I NaeI NarI NdeI NgoMIV PasI PfoI PmlI
PpuMI PspOMI PstI RsrII SacI SacII SanDI SapI
SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AhdI AleI
AlfI AloI ApaLI ApoI AseI AsiSI AvrII BbsI
BbvCI BciVI BclI BfrBI BglII BlpI BmrI BmtI
Bpu10I BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoNI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI MfeI MluI MmeI MslI NcoI NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII SalI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SspI SwaI TatI TstI Tth111I XbaI XhoI
XmnI ZraI