Restriction map for
pFN21AB8573
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
AspEI AviII AvrII BbeI BbrPI
BclI BfrI BlnI BsaAI BsaHI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrGI BssHII
BssSI BstEII BstPI BstUI Bsu36I
CpoI DraI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KspI
MaeII MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NgoMIV
NotI NruI PacI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AgeI 1349 ACCGGT A^CCGGT
Aor51HI 1238 AGCGCT AGC^GCT
AspHI 1684 GAGCTC G[AT]GC[AT]^C
AspI 46 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1394 CTCGAG C^[CT]CG[GA]G
BcgI 761 GCACCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1591 GTATCC GTATCC(6/5)
BglI 569 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2431 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2431 GCTGAGC GC^T[ACGT]AGC
BsaBI 1547 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 280 GAGACC GGTCTC(1/5)
BsaWI 1349 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1684 GAGCTC G[AT]GC[AT]^C
BsmBI 135 CGTCTC CGTCTC(1/5)
BsoBI 1394 CTCGAG C^[CT]CG[GA]G
BspDI 842 ATCGAT AT^CGAT
BsrBI 1927 GAGCGG CCGCTC(-3/-3)
BsrFI 1349 ACCGGT [GA]^CCGG[CT]
Bst1107I 199 GTATAC GTA^TAC
BstBI 2549 TTCGAA TT^CGAA
BstZ17I 199 GTATAC GTA^TAC
CelII 2431 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1349 ACCGGT [GA]^CCGG[CT]
ClaI 842 ATCGAT AT^CGAT
DraII 214 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1214 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 124 GACTGCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1238 AGCGCT AGC^GCT
EcoO109I 214 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1756 ATGCAT ATGCA^T
HincII 1389 GTTGAC GT[CT]^[GA]AC
HindII 1389 GTTGAC GT[CT]^[GA]AC
MamI 1547 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 473 CATATG CA^TATG
NheI 1510 GCTAGC G^CTAGC
NsiI 1756 ATGCAT ATGCA^T
NspV 2549 TTCGAA TT^CGAA
PaeR7I 1394 CTCGAG C^TCGAG
PflFI 46 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2358 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1349 ACCGGT A^CCGGT
PshAI 1976 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1684 GAGCTC GAGCT^C
SexAI 1364 ACCTGGT A^CC[AT]GGT
SfuI 2549 TTCGAA TT^CGAA
SphI 1524 GCATGC GCATG^C
Tth111I 46 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2358 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1394 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 199 GTATAC GT^[AC][GT]AC
733 GTCTAC
AcsI 1772 AAATTT [GA]^AATT[CT]
2553 AAATTT
ApoI 1772 AAATTT [GA]^AATT[CT]
2553 AAATTT
BalI 342 TGGCCA TGG^CCA
1490 TGGCCA
BanII 787 GGGCTC G[GA]GC[CT]^C
1684 GAGCTC
BbsI 1710 GAAGAC GAAGAC(2/6)
1583 GTCTTC
BbvCI 2162 CCTCAGC CCTCAGC(-5/-2)
1054 GCTGAGG
BpmI 1128 CTCCAG CTGGAG(16/14)
1750 CTGGAG
BpuAI 1710 GAAGAC GAAGAC(2/6)
1583 GTCTTC
BsgI 1558 CTGCAC GTGCAG(16/14)
1818 GTGCAG
EaeI 342 TGGCCA [CT]^GGCC[GA]
1490 TGGCCA
EcoNI 693 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
719 CCTTGAAGAGG
MluNI 342 TGGCCA TGG^CCA
1490 TGGCCA
MscI 342 TGGCCA TGG^CCA
1490 TGGCCA
MslI 1662 CATCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2181 CATTGATGTG
NspI 1524 GCATGC [GA]CATG^[CT]
2422 GCATGT
SapI 321 GAAGAGC GCTCTTC(1/4)
1945 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
AlwNI Asp700I Asp718I AvaII BamHI
BanI BbvI BcnI BfaI BglII
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI