(Circular) (Six-base) MAPSORT of: pFN21AB8573.seq Check: 1533 from: 1 to: 7375 pFN21AB8573 7375 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5185 278 Size: 53 83 186 936 3649 2468 Fragments arranged by size: 3649 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2205 2739 4718 1937 Size: 268 534 1979 4594 Fragments arranged by size: 4594 1979 534 268 Acc65I G'GTAC_C Cuts at: 2519 2759 4496 4696 2519 Size: 240 1737 200 5198 Fragments arranged by size: 5198 1737 240 200 AclI AA'CG_TT Cuts at: 1563 5502 5875 1563 Size: 3939 373 3063 Fragments arranged by size: 3939 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2217 2345 2627 3161 3575 4055 4146 4232 Size: 585 128 282 534 414 480 91 86 Cuts at: 4232 5438 6822 7090 1632 Size: 1206 1384 268 1917 Fragments arranged by size: 1917 1384 1206 585 534 480 414 282 268 128 91 86 AfeI AGC'GCT Cuts at: 3245 3245 Size: 7375 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7178 AflIII A'CryG_T Cuts at: 1203 6274 7251 1203 Size: 5071 977 1327 Fragments arranged by size: 5071 1327 977 AgeI A'CCGG_T Cuts at: 3354 6206 3354 Size: 2852 4523 AhdI GACnn_n'nnGTC Cuts at: 6104 6104 Size: 7375 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7375 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2135 1184 Size: 951 6424 AlwNI CAG_nnn'CTG Cuts at: 2867 3370 4013 4052 4369 5118 6690 2867 Size: 503 643 39 317 749 1572 3552 Fragments arranged by size: 3552 1572 749 643 503 317 39 ApaLI G'TGCA_C Cuts at: 5432 6588 5432 Size: 1156 6219 ApoI r'AATT_y Cuts at: 1493 3777 4558 4684 4910 5062 7171 1493 Size: 2284 781 126 226 152 2109 1697 Fragments arranged by size: 2284 2109 1697 781 226 152 126 AseI AT'TA_AT Cuts at: 160 1032 5929 160 Size: 872 4897 1606 Fragments arranged by size: 4897 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7375 AvaI C'yCGr_G Cuts at: 1870 1943 3399 4700 1870 Size: 73 1456 1301 4545 Fragments arranged by size: 4545 1456 1301 73 BamHI G'GATC_C Cuts at: 2016 3165 4617 4705 5072 2016 Size: 1149 1452 88 367 4319 Fragments arranged by size: 4319 1452 1149 367 88 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2519 2759 4496 4696 6151 Size: 325 217 627 732 240 1737 200 1455 Cuts at: 6151 618 Size: 1842 Fragments arranged by size: 1842 1737 1455 732 627 325 240 217 200 BanII G_rGCy'C Cuts at: 1391 2796 3693 4694 1391 Size: 1405 897 1001 4072 Fragments arranged by size: 4072 1405 1001 897 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3581 3722 928 Size: 971 1682 141 4581 Fragments arranged by size: 4581 1682 971 141 BbvCI CC'TCA_GC Cuts at: 3060 4168 3060 Size: 1108 6267 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2755 2789 5566 5600 1903 Size: 34 818 34 2777 34 3678 Fragments arranged by size: 3678 2777 818 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3607 5267 6477 3607 Size: 1660 1210 4505 Fragments arranged by size: 4505 1660 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7375 BfrBI ATG'CAT Cuts at: 3763 3763 Size: 7375 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2580 5986 136 Size: 107 122 71 631 605 908 3406 1525 Fragments arranged by size: 3406 1525 908 631 605 122 107 71 BglII A'GATC_T Cuts at: 2379 2925 3031 7370 2379 Size: 546 106 4339 2384 Fragments arranged by size: 4339 2384 546 106 BlpI GC'TnA_GC Cuts at: 4437 4792 4437 Size: 355 7020 Bme1580I G_kGCm'C Cuts at: 1790 2857 5436 6592 1790 Size: 1067 2579 1156 2573 Fragments arranged by size: 2579 2573 1156 1067 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7375 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3557 4311 5106 Size: 416 53 83 399 24 2140 754 795 Cuts at: 5106 6064 442 Size: 958 1753 Fragments arranged by size: 2140 1753 958 795 754 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3519 1056 Size: 2463 4912 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2558 4132 2558 Size: 1574 5801 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3118 3776 6035 1239 Size: 1879 658 2259 2579 Fragments arranged by size: 2579 2259 1879 658 Bpu10I CC'TnA_GC Cuts at: 3060 3621 4168 3060 Size: 561 547 6267 Fragments arranged by size: 6267 561 547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2230 3875 5500 6365 6663 2230 Size: 1645 1625 865 298 2942 Fragments arranged by size: 2942 1645 1625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2279 6038 882 Size: 617 780 3759 2219 Fragments arranged by size: 3759 2219 780 617 BsaAI yAC'GTr Cuts at: 493 1204 5166 493 Size: 711 3962 2702 Fragments arranged by size: 3962 2702 711 BsaBI GATnn'nnATC Cuts at: 3556 7369 3556 Size: 3813 3562 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5182 5564 6199 275 Size: 53 83 186 936 3649 382 635 1451 Fragments arranged by size: 3649 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3354 5808 6206 6480 6627 7038 3354 Size: 2454 398 274 147 411 3691 Fragments arranged by size: 3691 2454 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2699 2729 3116 3146 3180 Size: 30 165 30 1296 30 387 30 34 Cuts at: 3180 3210 3851 3881 1178 Size: 30 641 30 4672 Fragments arranged by size: 4672 1296 641 387 165 34 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2720 2723 2746 3077 3701 1186 Size: 1534 3 23 331 624 4860 Fragments arranged by size: 4860 1534 624 331 23 3 BseYI C'CCAG_C Cuts at: 2789 3602 4062 6578 7074 2789 Size: 813 460 2516 496 3090 Fragments arranged by size: 3090 2516 813 496 460 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3548 3844 1163 Size: 573 1812 296 4694 Fragments arranged by size: 4694 1812 573 296 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4825 5586 6614 1540 Size: 275 186 2824 761 1028 2301 Fragments arranged by size: 2824 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3693 5436 5521 6592 3693 Size: 1743 85 1071 4476 Fragments arranged by size: 4476 1743 1071 85 BsmI GAATG_Cn' Cuts at: 4886 4979 4886 Size: 93 7282 BsmBI CGTCTCn'nnnn_ Cuts at: 2146 2146 Size: 7375 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2796 2857 3693 4694 5436 5521 6592 Size: 399 1006 61 836 1001 742 85 1071 Cuts at: 6592 1391 Size: 2174 Fragments arranged by size: 2174 1071 1006 1001 836 742 399 85 61 BspEI T'CCGG_A Cuts at: 7038 7038 Size: 7375 BspHI T'CATG_A Cuts at: 5264 6994 5264 Size: 1730 5645 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2127 2158 4589 4730 844 Size: 1283 31 2431 141 3489 Fragments arranged by size: 3489 2431 1283 141 31 BsrBI CCG'CTC Cuts at: 1394 1823 3934 5262 1394 Size: 429 2111 1328 3507 Fragments arranged by size: 3507 2111 1328 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2338 2784 3510 3790 5870 6044 65 Size: 1183 1090 446 726 280 2080 174 1396 Fragments arranged by size: 2080 1396 1183 1090 726 446 280 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3354 6019 6206 1700 Size: 110 1544 2665 187 2869 Fragments arranged by size: 2869 2665 1544 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7375 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7375 BssSI C'ACGA_G Cuts at: 5435 6447 5435 Size: 1012 6363 BstAPI GCAn_nnn'nTGC Cuts at: 794 2989 4052 4433 794 Size: 2195 1063 381 3736 Fragments arranged by size: 3736 2195 1063 381 BstBI TT'CG_AA Cuts at: 4555 6179 4555 Size: 1624 5751 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7375 BstXI CCAn_nnnn'nTGG Cuts at: 2384 4192 4301 2384 Size: 1808 109 5458 Fragments arranged by size: 5458 1808 109 BstYI r'GATC_y Cuts at: 1972 2016 2379 2652 2910 2925 3031 3165 4617 Size: 44 363 273 258 15 106 134 1452 Cuts at: 4617 4705 5072 5457 5474 6926 7012 7024 7370 Size: 88 367 385 17 1452 86 12 346 Cuts at: 7370 1972 Size: 1977 Fragments arranged by size: 1977 1452 1452 385 367 363 346 273 258 134 106 88 86 44 17 15 12 BstZ17I GTA'TAC Cuts at: 2206 2206 Size: 7375 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6823 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6636 Fragments arranged by size: 6636 683 56 BtsI GCAGTG_nn' Cuts at: 2865 3719 3832 4103 4891 5685 5705 2865 Size: 854 113 271 788 794 20 4535 Fragments arranged by size: 4535 854 794 788 271 113 20 ClaI AT'CG_AT Cuts at: 1554 2848 1554 Size: 1294 6081 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7340 DraI TTT'AAA Cuts at: 4679 4679 Size: 7375 DraIII CAC_nnn'GTG Cuts at: 3224 3224 Size: 7375 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2135 6382 809 Size: 507 819 4247 1802 Fragments arranged by size: 4247 1802 819 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2347 3495 4822 Size: 54 1425 50 275 18 517 1148 1327 Cuts at: 4822 5711 7340 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1327 1148 889 517 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4822 1537 Size: 275 3010 4090 Fragments arranged by size: 4090 3010 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2321 2723 2803 3254 3492 3915 3945 4122 Size: 968 402 80 451 238 423 30 177 Cuts at: 4122 4203 5305 1353 Size: 81 1102 3423 Fragments arranged by size: 3423 1102 968 451 423 402 238 177 81 80 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2016 5962 6336 6482 387 Size: 1064 138 427 3946 374 146 1280 Fragments arranged by size: 3946 1280 1064 427 374 146 138 EcoICRI GAG'CTC Cuts at: 3691 3691 Size: 7375 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 2345 2627 3118 3161 3575 3776 Size: 393 585 128 282 491 43 414 201 Cuts at: 3776 4055 4146 4232 5438 6035 6822 7090 1239 Size: 279 91 86 1206 597 787 268 1524 Fragments arranged by size: 1524 1206 787 597 585 491 414 393 282 279 268 201 128 91 86 43 EcoNI CCTnn'n_nnAGG Cuts at: 2702 2728 2702 Size: 26 7349 EcoO109I rG'GnC_Cy Cuts at: 2220 2220 Size: 7375 EcoRI G'AATT_C Cuts at: 4684 5062 4684 Size: 378 6997 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2036 2603 2036 Size: 567 6808 FspI TGC'GCA Cuts at: 5881 5881 Size: 7375 HaeII r_GCGC'y Cuts at: 1678 2561 3247 4492 6522 1678 Size: 883 686 1245 2030 2531 Fragments arranged by size: 2531 2030 1245 883 686 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 2084 2116 2699 Size: 32 135 32 436 32 44 32 583 Cuts at: 2699 2731 3180 3212 6016 6048 6090 6122 1373 Size: 32 449 32 2804 32 42 32 2626 Fragments arranged by size: 2804 2626 583 449 436 135 44 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3396 4719 4965 669 Size: 988 281 1458 1323 246 3079 Fragments arranged by size: 3079 1458 1323 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7375 HpaI GTT'AAC Cuts at: 4965 4965 Size: 7375 KpnI G_GTAC'C Cuts at: 2523 2763 4500 4700 2523 Size: 240 1737 200 5198 Fragments arranged by size: 5198 1737 240 200 MfeI C'AATT_G Cuts at: 4974 4974 Size: 7375 MluI A'CGCG_T Cuts at: 7251 7251 Size: 7375 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2220 2618 2680 2732 6489 6673 1305 Size: 915 398 62 52 3757 184 2007 Fragments arranged by size: 3757 2007 915 398 184 62 52 MscI TGG'CCA Cuts at: 10 64 1489 1832 2349 3497 10 Size: 54 1425 343 517 1148 3888 Fragments arranged by size: 3888 1425 1148 517 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3671 4190 5333 5692 5851 518 Size: 648 2505 519 1143 359 159 2042 Fragments arranged by size: 2505 2042 1143 648 519 359 159 MspA1I CmG'CkG Cuts at: 1606 2033 2087 2830 3321 3754 3811 4791 5468 Size: 427 54 743 491 433 57 980 677 Cuts at: 5468 6616 6861 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 980 743 677 491 433 427 245 57 54 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6823 NdeI CA'TA_TG Cuts at: 387 2479 387 Size: 2092 5283 NheI G'CTAG_C Cuts at: 1052 3515 1052 Size: 2463 4912 NotI GC'GGCC_GC Cuts at: 4822 4822 Size: 7375 NsiI A_TGCA'T Cuts at: 3765 3765 Size: 7375 NspI r_CATG'y Cuts at: 1132 1229 3533 4431 4733 6278 1132 Size: 97 2304 898 302 1545 2229 Fragments arranged by size: 2304 2229 1545 898 302 97 PasI CC'CwG_GG Cuts at: 1348 3313 1348 Size: 1965 5410 PciI A'CATG_T Cuts at: 6274 6274 Size: 7375 PflMI CCAn_nnn'nTGG Cuts at: 1108 4369 1108 Size: 3261 4114 PmeI GTTT'AAAC Cuts at: 4679 4679 Size: 7375 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5444 5476 6957 6989 5444 Size: 32 1481 32 5830 Fragments arranged by size: 5830 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3985 1613 Size: 2372 5003 PsiI TTA'TAA Cuts at: 4945 4945 Size: 7375 PspXI vC'TCGA_Gb Cuts at: 3399 3399 Size: 7375 PstI C_TGCA'G Cuts at: 830 2092 4383 4604 4727 830 Size: 1262 2291 221 123 3478 Fragments arranged by size: 3478 2291 1262 221 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7375 PvuII CAG'CTG Cuts at: 2087 2830 3754 2087 Size: 743 924 5708 Fragments arranged by size: 5708 924 743 SacI G_AGCT'C Cuts at: 3693 3693 Size: 7375 SalI G'TCGA_C Cuts at: 1936 4717 1936 Size: 2781 4594 SapI GCTCTTCn'nnn_ Cuts at: 2321 3945 2321 Size: 1624 5751 SbfI CC_TGCA'GG Cuts at: 4727 4727 Size: 7375 ScaI AGT'ACT Cuts at: 5623 7335 5623 Size: 1712 5663 SexAI A'CCwGG_T Cuts at: 3369 3369 Size: 7375 SfcI C'TryA_G Cuts at: 826 1045 2088 3749 4379 4600 4723 5858 6539 Size: 219 1043 1661 630 221 123 1135 681 Cuts at: 6539 6730 826 Size: 191 1471 Fragments arranged by size: 1661 1471 1135 1043 681 630 221 219 191 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7375 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7375 SmaI CCC'GGG Cuts at: 1872 4702 1872 Size: 2830 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2209 3399 3890 5479 6380 6642 Size: 197 926 266 1190 491 1589 901 262 Cuts at: 6642 820 Size: 1553 Fragments arranged by size: 1589 1553 1190 926 901 491 266 262 197 SnaBI TAC'GTA Cuts at: 493 5166 493 Size: 4673 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7375 SphI G_CATG'C Cuts at: 1132 3533 4733 1132 Size: 2401 1200 3774 Fragments arranged by size: 3774 2401 1200 SspI AAT'ATT Cuts at: 5 52 5299 5 Size: 47 5247 2081 Fragments arranged by size: 5247 2081 47 StuI AGG'CCT Cuts at: 1517 2697 3068 3636 1517 Size: 1180 371 568 5256 Fragments arranged by size: 5256 1180 568 371 StyI C'CwwG_G Cuts at: 513 1065 2307 2578 3409 4465 4492 4814 513 Size: 552 1242 271 831 1056 27 322 3074 Fragments arranged by size: 3074 1242 1056 831 552 322 271 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2320 3131 3857 4076 5402 5419 5572 Size: 1255 740 811 726 219 1326 17 153 Cuts at: 5572 5757 6202 325 Size: 185 445 1498 Fragments arranged by size: 1498 1326 1255 811 740 726 445 219 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2446 5621 7333 Size: 274 80 33 51 1443 469 3175 1712 Cuts at: 7333 96 Size: 138 Fragments arranged by size: 3175 1712 1443 469 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2865 2897 3806 3838 7335 7367 2865 Size: 32 909 32 3497 32 2873 Fragments arranged by size: 3497 2873 909 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2054 1316 Size: 738 6637 XbaI T'CTAG_A Cuts at: 4711 4711 Size: 7375 XhoI C'TCGA_G Cuts at: 1943 3399 1943 Size: 1456 5919 XmaI C'CCGG_G Cuts at: 1870 4700 1870 Size: 2830 4545 XmnI GAAnn'nnTTC Cuts at: 3486 3924 4547 5504 3486 Size: 438 623 957 5357 Fragments arranged by size: 5357 957 623 438 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5183 276 Size: 53 83 186 936 3649 2468 Fragments arranged by size: 3649 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI