Restriction map for
pFN21AB8573
(Linear) (Six-base) MAPSORT of: KIBB8573.seq Check: 8480 from: 1 to: 2670
KIBB8573.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 0 200 734 2670
Size: 200 534 1936
Fragments arranged by size:
1936 534 200
Acc65I G'GTAC_C
Cuts at: 0 514 754 2491 2670
Size: 514 240 1737 179
Fragments arranged by size:
1737 514 240 179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 340 622 1156 1570 2050 2141 2227
Size: 212 128 282 534 414 480 91 86
Cuts at: 2227 2670
Size: 443
Fragments arranged by size:
534 480 443 414 282 212 128 91
86
AfeI AGC'GCT
Cuts at: 0 1240 2670
Size: 1240 1430
AgeI A'CCGG_T
Cuts at: 0 1349 2670
Size: 1349 1321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 130 2670
Size: 130 2540
AlwNI CAG_nnn'CTG
Cuts at: 0 862 1365 2008 2047 2364 2670
Size: 862 503 643 39 317 306
Fragments arranged by size:
862 643 503 317 306 39
ApoI r'AATT_y
Cuts at: 0 1772 2553 2670
Size: 1772 781 117
Fragments arranged by size:
1772 781 117
AvaI C'yCGr_G
Cuts at: 0 1394 2670
Size: 1394 1276
BamHI G'GATC_C
Cuts at: 0 11 1160 2612 2670
Size: 11 1149 1452 58
Fragments arranged by size:
1452 1149 58 11
BanI G'GyrC_C
Cuts at: 0 514 754 2491 2670
Size: 514 240 1737 179
Fragments arranged by size:
1737 514 240 179
BanII G_rGCy'C
Cuts at: 0 791 1688 2670
Size: 791 897 982
Fragments arranged by size:
982 897 791
BbsI GAAGACnn'nnnn_
Cuts at: 0 1576 1717 2670
Size: 1576 141 953
Fragments arranged by size:
1576 953 141
BbvCI CC'TCA_GC
Cuts at: 0 1055 2163 2670
Size: 1055 1108 507
Fragments arranged by size:
1108 1055 507
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 750 784 2670
Size: 750 34 1886
Fragments arranged by size:
1886 750 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1602 2670
Size: 1602 1068
BfrBI ATG'CAT
Cuts at: 0 1758 2670
Size: 1758 912
BglI GCCn_nnn'nGGC
Cuts at: 0 575 2670
Size: 575 2095
BglII A'GATC_T
Cuts at: 0 374 920 1026 2670
Size: 374 546 106 1644
Fragments arranged by size:
1644 546 374 106
BlpI GC'TnA_GC
Cuts at: 0 2432 2670
Size: 2432 238
Bme1580I G_kGCm'C
Cuts at: 0 852 2670
Size: 852 1818
BmrI ACTGGGnnnn_n'
Cuts at: 0 1552 2306 2670
Size: 1552 754 364
Fragments arranged by size:
1552 754 364
BmtI G_CTAG'C
Cuts at: 0 1514 2670
Size: 1514 1156
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 553 2127 2670
Size: 553 1574 543
Fragments arranged by size:
1574 553 543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1113 1771 2670
Size: 1113 658 899
Fragments arranged by size:
1113 899 658
Bpu10I CC'TnA_GC
Cuts at: 0 1055 1616 2163 2670
Size: 1055 561 547 507
Fragments arranged by size:
1055 561 547 507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 1870 2670
Size: 225 1645 800
Fragments arranged by size:
1645 800 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 274 2670
Size: 274 2396
BsaBI GATnn'nnATC
Cuts at: 0 1551 2670
Size: 1551 1119
BsaWI w'CCGG_w
Cuts at: 0 1349 2670
Size: 1349 1321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 694 724 1111 1141 1175 1205 1846 1876
Size: 694 30 387 30 34 30 641 30
Cuts at: 1876 2670
Size: 794
Fragments arranged by size:
794 694 641 387 34 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 715 718 741 1072 1696 2670
Size: 715 3 23 331 624 974
Fragments arranged by size:
974 715 624 331 23 3
BseYI C'CCAG_C
Cuts at: 0 784 1597 2057 2670
Size: 784 813 460 613
Fragments arranged by size:
813 784 613 460
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1543 1839 2670
Size: 1543 296 831
Fragments arranged by size:
1543 831 296
BsiHKAI G_wGCw'C
Cuts at: 0 1688 2670
Size: 1688 982
BsmBI CGTCTCn'nnnn_
Cuts at: 0 141 2670
Size: 141 2529
Bsp1286I G_dGCh'C
Cuts at: 0 791 852 1688 2670
Size: 791 61 836 982
Fragments arranged by size:
982 836 791 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 153 2584 2670
Size: 122 31 2431 86
Fragments arranged by size:
2431 122 86 31
BsrBI CCG'CTC
Cuts at: 0 1929 2670
Size: 1929 741
BsrDI GCAATG_nn'
Cuts at: 0 333 779 1505 1785 2670
Size: 333 446 726 280 885
Fragments arranged by size:
885 726 446 333 280
BsrFI r'CCGG_y
Cuts at: 0 1349 2670
Size: 1349 1321
BstAPI GCAn_nnn'nTGC
Cuts at: 0 984 2047 2428 2670
Size: 984 1063 381 242
Fragments arranged by size:
1063 984 381 242
BstBI TT'CG_AA
Cuts at: 0 2550 2670
Size: 2550 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 379 2187 2296 2670
Size: 379 1808 109 374
Fragments arranged by size:
1808 379 374 109
BstYI r'GATC_y
Cuts at: 0 11 374 647 905 920 1026 1160 2612
Size: 11 363 273 258 15 106 134 1452
Cuts at: 2612 2670
Size: 58
Fragments arranged by size:
1452 363 273 258 134 106 58 15
11
BstZ17I GTA'TAC
Cuts at: 0 201 2670
Size: 201 2469
BtsI GCAGTG_nn'
Cuts at: 0 860 1714 1827 2098 2670
Size: 860 854 113 271 572
Fragments arranged by size:
860 854 572 271 113
ClaI AT'CG_AT
Cuts at: 0 843 2670
Size: 843 1827
DraIII CAC_nnn'GTG
Cuts at: 0 1219 2670
Size: 1219 1451
DrdI GACnn_nn'nnGTC
Cuts at: 0 130 2670
Size: 130 2540
EaeI y'GGCC_r
Cuts at: 0 342 1490 2670
Size: 342 1148 1180
Fragments arranged by size:
1180 1148 342
EarI CTCTTCn'nnn_
Cuts at: 0 316 718 798 1249 1487 1910 1940 2117
Size: 316 402 80 451 238 423 30 177
Cuts at: 2117 2198 2670
Size: 81 472
Fragments arranged by size:
472 451 423 402 316 238 177 81
80 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 11 2670
Size: 11 2659
EcoICRI GAG'CTC
Cuts at: 0 1686 2670
Size: 1686 984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 340 622 1113 1156 1570 1771 2050
Size: 212 128 282 491 43 414 201 279
Cuts at: 2050 2141 2227 2670
Size: 91 86 443
Fragments arranged by size:
491 443 414 282 279 212 201 128
91 86 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 697 723 2670
Size: 697 26 1947
Fragments arranged by size:
1947 697 26
EcoO109I rG'GnC_Cy
Cuts at: 0 215 2670
Size: 215 2455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 31 598 2670
Size: 31 567 2072
Fragments arranged by size:
2072 567 31
HaeII r_GCGC'y
Cuts at: 0 556 1242 2487 2670
Size: 556 686 1245 183
Fragments arranged by size:
1245 686 556 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 79 111 694 726 1175 1207
Size: 3 32 44 32 583 32 449 32
Cuts at: 1207 2670
Size: 1463
Fragments arranged by size:
1463 583 449 44 32 32 32 32
3
HincII GTy'rAC
Cuts at: 0 1391 2670
Size: 1391 1279
KpnI G_GTAC'C
Cuts at: 0 518 758 2495 2670
Size: 518 240 1737 175
Fragments arranged by size:
1737 518 240 175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 613 675 727 2670
Size: 215 398 62 52 1943
Fragments arranged by size:
1943 398 215 62 52
MscI TGG'CCA
Cuts at: 0 344 1492 2670
Size: 344 1148 1178
Fragments arranged by size:
1178 1148 344
MslI CAynn'nnrTG
Cuts at: 0 1666 2185 2670
Size: 1666 519 485
Fragments arranged by size:
1666 519 485
MspA1I CmG'CkG
Cuts at: 0 28 82 825 1316 1749 1806 2670
Size: 28 54 743 491 433 57 864
Fragments arranged by size:
864 743 491 433 57 54 28
NdeI CA'TA_TG
Cuts at: 0 474 2670
Size: 474 2196
NheI G'CTAG_C
Cuts at: 0 1510 2670
Size: 1510 1160
NsiI A_TGCA'T
Cuts at: 0 1760 2670
Size: 1760 910
NspI r_CATG'y
Cuts at: 0 1528 2426 2670
Size: 1528 898 244
Fragments arranged by size:
1528 898 244
PasI CC'CwG_GG
Cuts at: 0 1308 2670
Size: 1308 1362
PflMI CCAn_nnn'nTGG
Cuts at: 0 2364 2670
Size: 2364 306
PshAI GACnn'nnGTC
Cuts at: 0 1980 2670
Size: 1980 690
PspXI vC'TCGA_Gb
Cuts at: 0 1394 2670
Size: 1394 1276
PstI C_TGCA'G
Cuts at: 0 87 2378 2599 2670
Size: 87 2291 221 71
Fragments arranged by size:
2291 221 87 71
PvuII CAG'CTG
Cuts at: 0 82 825 1749 2670
Size: 82 743 924 921
Fragments arranged by size:
924 921 743 82
SacI G_AGCT'C
Cuts at: 0 1688 2670
Size: 1688 982
SapI GCTCTTCn'nnn_
Cuts at: 0 316 1940 2670
Size: 316 1624 730
Fragments arranged by size:
1624 730 316
SexAI A'CCwGG_T
Cuts at: 0 1364 2670
Size: 1364 1306
SfcI C'TryA_G
Cuts at: 0 83 1744 2374 2595 2670
Size: 83 1661 630 221 75
Fragments arranged by size:
1661 630 221 83 75
SmlI C'TyrA_G
Cuts at: 0 204 1394 1885 2670
Size: 204 1190 491 785
Fragments arranged by size:
1190 785 491 204
SphI G_CATG'C
Cuts at: 0 1528 2670
Size: 1528 1142
StuI AGG'CCT
Cuts at: 0 692 1063 1631 2670
Size: 692 371 568 1039
Fragments arranged by size:
1039 692 568 371
StyI C'CwwG_G
Cuts at: 0 302 573 1404 2460 2487 2670
Size: 302 271 831 1056 27 183
Fragments arranged by size:
1056 831 302 271 183 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 315 1126 1852 2071 2670
Size: 315 811 726 219 599
Fragments arranged by size:
811 726 599 315 219
TatI w'GTAC_w
Cuts at: 0 441 2670
Size: 441 2229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 860 892 1801 1833 2670
Size: 860 32 909 32 837
Fragments arranged by size:
909 860 837 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 49 2670
Size: 49 2621
XhoI C'TCGA_G
Cuts at: 0 1394 2670
Size: 1394 1276
XmnI GAAnn'nnTTC
Cuts at: 0 1481 1919 2542 2670
Size: 1481 438 623 128
Fragments arranged by size:
1481 623 438 128
Enzymes that do cut:
AccI Acc65I AcuI AfeI AgeI AlfI AlwNI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstAPI BstBI BstXI BstYI BstZ17I BtsI ClaI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII KpnI MmeI MscI
MslI MspA1I NdeI NheI NsiI NspI PasI PflMI
PshAI PspXI PstI PvuII SacI SapI SexAI SfcI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BclI BmgBI BsaAI BsaHI BsiEI BsiWI BsmI BspEI
BspHI BsrGI BssHII BssSI BstEII Bsu36I BtgI BtgZI
CspCI DraI EagI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI MfeI MluI NaeI NarI NcoI
NgoMIV NotI NruI PacI PciI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI XbaI XcmI
XmaI ZraI