################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbeI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstZ17I Bsu36I ClaI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SexAI SfiI SfoI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AviII 1161 TGCGCA TGC^GCA BalI 113 TGGCCA TGG^CCA BbsI 169 GTCTTC GAAGAC(2/6) BcgI 923 CGAAGGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 169 GTCTTC GAAGAC(2/6) BsiEI 2472 CGGCCG CG[GA][CT]^CG BsmBI 1009 GAGACG CGTCTC(1/5) BsrBI 1188 GAGCGG CCGCTC(-3/-3) BssHII 2451 GCGCGC G^CGCGC BstBI 921 TTCGAA TT^CGAA BstEII 2244 GGTGACC G^GT[ACGT]ACC BstPI 2244 GGTGACC G^GT[ACGT]ACC CpoI 2020 CGGTCCG CG^G[AT]CCG DsaI 1560 CCATGG C^C[GA][CT]GG EagI 2472 CGGCCG C^GGCCG EclXI 2472 CGGCCG C^GGCCG Eco52I 2472 CGGCCG C^GGCCG EcoNI 2310 CCTTCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2244 GGTGACC G^GT[ACGT]ACC FspI 1161 TGCGCA TGC^GCA MaeII 2432 ACGT A^CGT MluNI 113 TGGCCA TGG^CCA MscI 113 TGGCCA TGG^CCA NcoI 1560 CCATGG C^CATGG NspV 921 TTCGAA TT^CGAA RsrII 2020 CGGTCCG CG^G[AT]CCG SfuI 921 TTCGAA TT^CGAA SgrAI 2161 CACCGGCG C[GA]^CCGG[CT]G SphI 974 GCATGC GCATG^C Tsp509I 959 AATT ^AATT XbaI 1432 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 2452 CGCG CG^CG 2454 CGCG AcyI 1011 GACGCC G[GA]^CG[CT]C 2681 GGCGTC BglI 2288 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2623 GCCCGGACGGC BsaHI 1011 GACGCC G[GA]^CG[CT]C 2681 GGCGTC BsgI 517 CTGCAC GTGCAG(16/14) 2210 GTGCAG BsmI 132 GAATGC GAATGC(1/-1) 324 GAATGC BstUI 2452 CGCG CG^CG 2454 CGCG BstXI 2075 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2222 CCAAGCAGCTGG Hin1I 1011 GACGCC G[GA]^CG[CT]C 2681 GGCGTC MvnI 2452 CGCG CG^CG 2454 CGCG NaeI 2474 GCCGGC GCC^GGC 2754 GCCGGC NgoMIV 2474 GCCGGC G^CCGGC 2754 GCCGGC PpuMI 1239 GGGACCC [GA]G^G[AT]CC[CT] 1922 AGGACCT SacI 428 GAGCTC GAGCT^C 2671 GAGCTC SapI 2643 GAAGAGC GCTCTTC(1/4) 2325 GCTCTTC TaqI 922 TCGA T^CGA 1112 TCGA TfiI 462 GATTC G^A[AT]TC 2008 GATTC TthHB8I 922 TCGA T^CGA 1112 TCGA XcmI 1644 CCAGAGGGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2558 CCAATAAGACGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BamHI BanI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI Tru9I TseI Tsp45I TspRI Van91I XhoII XmaCI XmaI