(Circular) (Six-base) MAPSORT of: pFN21AB8412.seq Check: 884 from: 1 to: 7474 pFN21AB8412 7474 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3127 3127 Size: 7474 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5284 278 Size: 53 83 186 936 3748 2468 Fragments arranged by size: 3748 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4817 1937 Size: 2880 4594 Acc65I G'GTAC_C Cuts at: 4795 4795 Size: 7474 AclI AA'CG_TT Cuts at: 1563 4773 5601 5974 1563 Size: 3210 828 373 3063 Fragments arranged by size: 3210 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 2390 3053 3245 3869 4052 4265 4538 Size: 503 255 663 192 624 183 213 273 Cuts at: 4538 4575 5537 6921 7189 1632 Size: 37 962 1384 268 1917 Fragments arranged by size: 1917 1384 962 663 624 503 273 268 255 213 192 183 37 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7277 AflIII A'CryG_T Cuts at: 1203 6373 7350 1203 Size: 5170 977 1327 Fragments arranged by size: 5170 1327 977 AgeI A'CCGG_T Cuts at: 6305 6305 Size: 7474 AhdI GACnn_n'nnGTC Cuts at: 6203 6203 Size: 7474 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7474 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3360 1184 Size: 2176 5298 AlwNI CAG_nnn'CTG Cuts at: 2882 3142 3235 4385 4553 4594 4666 5217 6789 Size: 260 93 1150 168 41 72 551 1572 Cuts at: 6789 2882 Size: 3567 Fragments arranged by size: 3567 1572 1150 551 260 168 93 72 41 ApaI G_GGCC'C Cuts at: 2090 2561 3824 2090 Size: 471 1263 5740 Fragments arranged by size: 5740 1263 471 ApaLI G'TGCA_C Cuts at: 5531 6687 5531 Size: 1156 6318 ApoI r'AATT_y Cuts at: 1493 4783 5009 5161 7270 1493 Size: 3290 226 152 2109 1697 Fragments arranged by size: 3290 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6028 160 Size: 872 4996 1606 Fragments arranged by size: 4996 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7474 AvaI C'yCGr_G Cuts at: 1870 1943 2553 2950 3909 4319 4799 1870 Size: 73 610 397 959 410 480 4545 Fragments arranged by size: 4545 959 610 480 410 397 73 BamHI G'GATC_C Cuts at: 2424 2844 4090 4185 4203 4804 5171 2424 Size: 420 1246 95 18 601 367 4727 Fragments arranged by size: 4727 1246 601 420 367 95 18 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2145 3403 4762 4795 6250 Size: 325 217 627 358 1258 1359 33 1455 Cuts at: 6250 618 Size: 1842 Fragments arranged by size: 1842 1455 1359 1258 627 358 325 217 33 BanII G_rGCy'C Cuts at: 1391 2090 2437 2561 2637 2895 3798 3824 4680 Size: 699 347 124 76 258 903 26 856 Cuts at: 4680 4793 1391 Size: 113 4072 Fragments arranged by size: 4072 903 856 699 347 258 124 113 76 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2167 928 Size: 971 268 6235 Fragments arranged by size: 6235 971 268 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2917 2951 5665 5699 1903 Size: 34 980 34 2714 34 3678 Fragments arranged by size: 3678 2714 980 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5366 6576 5366 Size: 1210 6264 BclI T'GATC_A Cuts at: 1548 1548 Size: 7474 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4299 4634 6085 Size: 107 122 71 631 605 2627 335 1451 Cuts at: 6085 136 Size: 1525 Fragments arranged by size: 2627 1525 1451 631 605 335 122 107 71 BglII A'GATC_T Cuts at: 7469 7469 Size: 7474 BlpI GC'TnA_GC Cuts at: 2436 2889 3003 4891 2436 Size: 453 114 1888 5019 Fragments arranged by size: 5019 1888 453 114 Bme1580I G_kGCm'C Cuts at: 1790 2090 2325 2561 3824 5535 6691 1790 Size: 300 235 236 1263 1711 1156 2573 Fragments arranged by size: 2573 1711 1263 1156 300 236 235 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7474 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2005 5205 6163 Size: 416 53 83 399 24 588 3200 958 Cuts at: 6163 442 Size: 1753 Fragments arranged by size: 3200 1753 958 588 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7474 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2717 2859 3441 3632 3659 4028 4532 4544 Size: 1478 142 582 191 27 369 504 12 Cuts at: 4544 4643 4727 6134 1239 Size: 99 84 1407 2579 Fragments arranged by size: 2579 1478 1407 582 504 369 191 142 99 84 27 12 Bpu10I CC'TnA_GC Cuts at: 2528 2810 2528 Size: 282 7192 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5599 6464 6762 5599 Size: 865 298 6311 Fragments arranged by size: 6311 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2857 3102 4616 6137 882 Size: 617 1358 245 1514 1521 2219 Fragments arranged by size: 2219 1521 1514 1358 617 245 BsaAI yAC'GTr Cuts at: 493 1204 5265 493 Size: 711 4061 2702 Fragments arranged by size: 4061 2702 711 BsaBI GATnn'nnATC Cuts at: 7468 7468 Size: 7474 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3017 4687 5281 5663 Size: 53 83 186 936 1484 1670 594 382 Cuts at: 5663 6298 275 Size: 635 1451 Fragments arranged by size: 1670 1484 1451 936 635 594 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2463 4023 4272 5907 6305 6579 6726 7137 2463 Size: 1560 249 1635 398 274 147 411 2800 Fragments arranged by size: 2800 1635 1560 411 398 274 249 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2585 2615 2951 2981 3314 Size: 30 165 30 1182 30 336 30 333 Cuts at: 3314 3344 4010 4040 1178 Size: 30 666 30 4612 Fragments arranged by size: 4612 1182 666 336 333 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2082 2100 2376 3083 3329 3746 3794 3998 Size: 896 18 276 707 246 417 48 204 Cuts at: 3998 4001 4337 4541 4634 4769 1186 Size: 3 336 204 93 135 3891 Fragments arranged by size: 3891 896 707 417 336 276 246 204 204 135 93 48 18 3 BseYI C'CCAG_C Cuts at: 2268 2272 2377 3052 4740 6677 7173 2268 Size: 4 105 675 1688 1937 496 2569 Fragments arranged by size: 2569 1937 1688 675 496 105 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2507 4236 1163 Size: 573 771 1729 4401 Fragments arranged by size: 4401 1729 771 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4480 4924 5685 6713 1540 Size: 275 186 2479 444 761 1028 2301 Fragments arranged by size: 2479 2301 1028 761 444 275 186 BsiHKAI G_wGCw'C Cuts at: 2437 4107 4332 4680 5535 5620 6691 2437 Size: 1670 225 348 855 85 1071 3220 Fragments arranged by size: 3220 1670 1071 855 348 225 85 BsmI GAATG_Cn' Cuts at: 2143 2335 4985 5078 2143 Size: 192 2650 93 4539 Fragments arranged by size: 4539 2650 192 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3008 3008 Size: 7474 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2325 2437 2561 2637 2895 3798 Size: 399 300 235 112 124 76 258 903 Cuts at: 3798 3824 4107 4332 4680 4793 5535 5620 6691 Size: 26 283 225 348 113 742 85 1071 Cuts at: 6691 1391 Size: 2174 Fragments arranged by size: 2174 1071 903 742 399 348 300 283 258 235 225 124 113 112 85 76 26 BspEI T'CCGG_A Cuts at: 7137 7137 Size: 7474 BspHI T'CATG_A Cuts at: 5363 7093 5363 Size: 1730 5744 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2673 3127 3243 3288 3532 4356 4829 844 Size: 1829 454 116 45 244 824 473 3489 Fragments arranged by size: 3489 1829 824 473 454 244 116 45 BsrBI CCG'CTC Cuts at: 1394 1823 3195 5361 1394 Size: 429 1372 2166 3507 Fragments arranged by size: 3507 2166 1372 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5969 6143 65 Size: 1183 4721 174 1396 Fragments arranged by size: 4721 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4167 4479 4759 6118 6305 1700 Size: 110 2357 312 280 1359 187 2869 Fragments arranged by size: 2869 2357 1359 312 280 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7474 BssHII G'CGCG_C Cuts at: 1925 4456 1925 Size: 2531 4943 BssSI C'ACGA_G Cuts at: 5534 6546 5534 Size: 1012 6462 BstAPI GCAn_nnn'nTGC Cuts at: 794 2665 3410 4594 794 Size: 1871 745 1184 3674 Fragments arranged by size: 3674 1871 1184 745 BstBI TT'CG_AA Cuts at: 2927 6278 2927 Size: 3351 4123 BstEII G'GTnAC_C Cuts at: 1184 4249 1184 Size: 3065 4409 BstXI CCAn_nnnn'nTGG Cuts at: 4087 4234 4087 Size: 147 7327 BstYI r'GATC_y Cuts at: 1972 2424 2844 3600 4090 4185 4203 4500 4710 Size: 452 420 756 490 95 18 297 210 Cuts at: 4710 4804 5171 5556 5573 7025 7111 7123 7469 Size: 94 367 385 17 1452 86 12 346 Cuts at: 7469 1972 Size: 1977 Fragments arranged by size: 1977 1452 756 490 452 420 385 367 346 297 210 95 94 86 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 3565 513 Size: 552 2500 4422 Fragments arranged by size: 4422 2500 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3517 487 Size: 683 56 2291 4444 Fragments arranged by size: 4444 2291 683 56 BtsI GCAGTG_nn' Cuts at: 2325 2519 2898 3081 4990 5784 5804 2325 Size: 194 379 183 1909 794 20 3995 Fragments arranged by size: 3995 1909 794 379 194 183 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7474 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4135 4170 529 Size: 35 3571 35 3833 Fragments arranged by size: 3833 3571 35 35 DraI TTT'AAA Cuts at: 4778 4778 Size: 7474 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6481 809 Size: 507 5165 1802 Fragments arranged by size: 5165 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 3624 3738 Size: 54 1425 50 275 18 288 1506 114 Cuts at: 3738 4477 4921 5810 7439 8 Size: 739 444 889 1629 43 Fragments arranged by size: 1629 1506 1425 889 739 444 288 275 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4477 4921 1537 Size: 275 2665 444 4090 Fragments arranged by size: 4090 2665 444 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2085 2220 2394 2507 2860 2950 3060 3886 Size: 732 135 174 113 353 90 110 826 Cuts at: 3886 4066 4337 4643 5404 1353 Size: 180 271 306 761 3423 Fragments arranged by size: 3423 826 761 732 353 306 271 180 174 135 113 110 90 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4625 4652 6061 6435 6581 387 Size: 1064 138 3036 27 1409 374 146 1280 Fragments arranged by size: 3036 1409 1280 1064 374 146 138 27 EcoICRI GAG'CTC Cuts at: 2435 4678 2435 Size: 2243 5231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2390 2717 2859 3053 3245 3441 Size: 393 503 255 327 142 194 192 196 Cuts at: 3441 3632 3659 3869 4028 4052 4265 4532 4538 Size: 191 27 210 159 24 213 267 6 Cuts at: 4538 4544 4575 4643 4727 5537 6134 6921 7189 Size: 6 31 68 84 810 597 787 268 Cuts at: 7189 1239 Size: 1524 Fragments arranged by size: 1524 810 787 597 503 393 327 268 267 255 213 210 196 194 192 191 159 142 84 68 31 27 24 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 4319 4319 Size: 7474 EcoO109I rG'GnC_Cy Cuts at: 2087 2557 2558 3245 3325 3502 3820 3841 3928 Size: 470 1 687 80 177 318 21 87 Cuts at: 3928 4300 2087 Size: 372 5261 Fragments arranged by size: 5261 687 470 372 318 177 87 80 21 1 EcoRI G'AATT_C Cuts at: 4783 5161 4783 Size: 378 7096 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3621 3621 Size: 7474 FspI TGC'GCA Cuts at: 3168 5980 3168 Size: 2812 4662 HaeII r_GCGC'y Cuts at: 1678 2023 2740 3217 6621 1678 Size: 345 717 477 3404 2531 Fragments arranged by size: 3404 2531 717 477 345 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2585 2617 2759 2791 4010 Size: 32 135 32 1013 32 142 32 1219 Cuts at: 4010 4042 6115 6147 6189 6221 1373 Size: 32 2073 32 42 32 2626 Fragments arranged by size: 2626 2073 1219 1013 142 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4818 5064 669 Size: 988 281 2880 246 3079 Fragments arranged by size: 3079 2880 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7474 HpaI GTT'AAC Cuts at: 5064 5064 Size: 7474 KpnI G_GTAC'C Cuts at: 4799 4799 Size: 7474 MfeI C'AATT_G Cuts at: 5073 5073 Size: 7474 MluI A'CGCG_T Cuts at: 7350 7350 Size: 7474 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2379 2553 2937 6588 6772 1305 Size: 1074 174 384 3651 184 2007 Fragments arranged by size: 3651 2007 1074 384 184 174 MscI TGG'CCA Cuts at: 10 64 1489 1832 2120 10 Size: 54 1425 343 288 5364 Fragments arranged by size: 5364 1425 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 5432 5791 5950 518 Size: 648 4266 359 159 2042 Fragments arranged by size: 4266 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2170 2272 2620 3162 3847 4234 4476 Size: 420 144 102 348 542 685 387 242 Cuts at: 4476 4663 4705 4717 4740 4890 5567 6715 6960 Size: 187 42 12 23 150 677 1148 245 Cuts at: 6960 1606 Size: 2120 Fragments arranged by size: 2120 1148 685 677 542 420 387 348 245 242 187 150 144 102 42 23 12 NaeI GCC'GGC Cuts at: 4481 4761 4481 Size: 280 7194 NcoI C'CATG_G Cuts at: 513 1065 3565 513 Size: 552 2500 4422 Fragments arranged by size: 4422 2500 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7474 NgoMIV G'CCGG_C Cuts at: 4479 4759 4479 Size: 280 7194 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7474 NotI GC'GGCC_GC Cuts at: 4921 4921 Size: 7474 NspI r_CATG'y Cuts at: 1132 1229 2399 2983 4078 4832 6377 1132 Size: 97 1170 584 1095 754 1545 2229 Fragments arranged by size: 2229 1545 1170 1095 754 584 97 PasI CC'CwG_GG Cuts at: 1348 2407 2637 1348 Size: 1059 230 6185 Fragments arranged by size: 6185 1059 230 PciI A'CATG_T Cuts at: 6373 6373 Size: 7474 PflMI CCAn_nnn'nTGG Cuts at: 1108 2882 3210 4510 4720 1108 Size: 1774 328 1300 210 3862 Fragments arranged by size: 3862 1774 1300 328 210 PmeI GTTT'AAAC Cuts at: 4778 4778 Size: 7474 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5543 5575 7056 7088 4040 Size: 32 1471 32 1481 32 4426 Fragments arranged by size: 4426 1481 1471 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3245 3928 3245 Size: 683 6791 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7474 PsiI TTA'TAA Cuts at: 3675 5044 3675 Size: 1369 6105 PspOMI G'GGCC_C Cuts at: 2086 2557 3820 2086 Size: 471 1263 5740 Fragments arranged by size: 5740 1263 471 PstI C_TGCA'G Cuts at: 830 2905 3240 3349 4455 4674 4704 4826 830 Size: 2075 335 109 1106 219 30 122 3478 Fragments arranged by size: 3478 2075 1106 335 219 122 109 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7474 PvuII CAG'CTG Cuts at: 2272 4234 4705 4740 2272 Size: 1962 471 35 5006 Fragments arranged by size: 5006 1962 471 35 RsrII CG'GwC_CG Cuts at: 4026 4026 Size: 7474 SacI G_AGCT'C Cuts at: 2437 4680 2437 Size: 2243 5231 SalI G'TCGA_C Cuts at: 1936 4816 1936 Size: 2880 4594 SanDI GG'GwC_CC Cuts at: 3245 3245 Size: 7474 SapI GCTCTTCn'nnn_ Cuts at: 4337 4643 4337 Size: 306 7168 SbfI CC_TGCA'GG Cuts at: 4826 4826 Size: 7474 ScaI AGT'ACT Cuts at: 5722 7434 5722 Size: 1712 5762 SfcI C'TryA_G Cuts at: 826 1045 2901 3236 3345 3664 3828 4451 4670 Size: 219 1856 335 109 319 164 623 219 Cuts at: 4670 4700 4822 5957 6638 6829 826 Size: 30 122 1135 681 191 1471 Fragments arranged by size: 1856 1471 1135 681 623 335 319 219 219 191 164 122 109 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7474 SgrAI Cr'CCGG_yG Cuts at: 1700 4167 1700 Size: 2467 5007 SmaI CCC'GGG Cuts at: 1872 2555 2952 3911 4801 1872 Size: 683 397 959 890 4545 Fragments arranged by size: 4545 959 890 683 397 SmlI C'TyrA_G Cuts at: 820 1017 1943 5578 6479 6741 820 Size: 197 926 3635 901 262 1553 Fragments arranged by size: 3635 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5265 493 Size: 4772 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7474 SphI G_CATG'C Cuts at: 1132 2983 4832 1132 Size: 1851 1849 3774 Fragments arranged by size: 3774 1851 1849 SspI AAT'ATT Cuts at: 5 52 5398 5 Size: 47 5346 2081 Fragments arranged by size: 5346 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7474 StyI C'CwwG_G Cuts at: 513 1065 2654 3008 3414 3565 3741 3756 3798 Size: 552 1589 354 406 151 176 15 42 Cuts at: 3798 4080 4198 4407 4913 513 Size: 282 118 209 506 3074 Fragments arranged by size: 3074 1589 552 506 406 354 282 209 176 151 118 42 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2264 4732 5501 5518 5671 5856 6301 Size: 1255 684 2468 769 17 153 185 445 Cuts at: 6301 325 Size: 1498 Fragments arranged by size: 2468 1498 1255 769 684 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4284 5720 7432 Size: 274 80 33 51 1443 2307 1436 1712 Cuts at: 7432 96 Size: 138 Fragments arranged by size: 2307 1712 1443 1436 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3314 3346 7434 7466 3314 Size: 32 4088 32 3322 Fragments arranged by size: 4088 3322 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7474 XbaI T'CTAG_A Cuts at: 3437 4810 3437 Size: 1373 6101 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3656 4570 3656 Size: 914 6560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7474 XmaI C'CCGG_G Cuts at: 1870 2553 2950 3909 4799 1870 Size: 683 397 959 890 4545 Fragments arranged by size: 4545 959 890 683 397 XmnI GAAnn'nnTTC Cuts at: 5603 5603 Size: 7474 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5282 276 Size: 53 83 186 936 3748 2468 Fragments arranged by size: 3748 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI SacII SexAI SfiI SfoI SrfI SwaI