(Linear) (Six-base) MAPSORT of: KIBB8412.seq Check: 5258 from: 1 to: 2769 KIBB8412.orf With 182 enzymes: SgfI * December 5, 2014 12:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1122 2769 Size: 1122 1647 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 385 1048 1240 1864 2047 2260 2533 Size: 130 255 663 192 624 183 213 273 Cuts at: 2533 2570 2769 Size: 37 199 Fragments arranged by size: 663 624 273 255 213 199 192 183 130 37 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1355 2769 Size: 1355 1414 AlwNI CAG_nnn'CTG Cuts at: 0 877 1137 1230 2380 2548 2589 2661 2769 Size: 877 260 93 1150 168 41 72 108 Fragments arranged by size: 1150 877 260 168 108 93 72 41 ApaI G_GGCC'C Cuts at: 0 85 556 1819 2769 Size: 85 471 1263 950 Fragments arranged by size: 1263 950 471 85 AvaI C'yCGr_G Cuts at: 0 548 945 1904 2314 2769 Size: 548 397 959 410 455 Fragments arranged by size: 959 548 455 410 397 BamHI G'GATC_C Cuts at: 0 419 839 2085 2180 2198 2769 Size: 419 420 1246 95 18 571 Fragments arranged by size: 1246 571 420 419 95 18 BanI G'GyrC_C Cuts at: 0 140 1398 2757 2769 Size: 140 1258 1359 12 Fragments arranged by size: 1359 1258 140 12 BanII G_rGCy'C Cuts at: 0 85 432 556 632 890 1793 1819 2675 Size: 85 347 124 76 258 903 26 856 Cuts at: 2675 2769 Size: 94 Fragments arranged by size: 903 856 347 258 124 94 85 76 26 BbsI GAAGACnn'nnnn_ Cuts at: 0 162 2769 Size: 162 2607 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 912 946 2769 Size: 912 34 1823 Fragments arranged by size: 1823 912 34 BglI GCCn_nnn'nGGC Cuts at: 0 2294 2629 2769 Size: 2294 335 140 Fragments arranged by size: 2294 335 140 BlpI GC'TnA_GC Cuts at: 0 431 884 998 2769 Size: 431 453 114 1771 Fragments arranged by size: 1771 453 431 114 Bme1580I G_kGCm'C Cuts at: 0 85 320 556 1819 2769 Size: 85 235 236 1263 950 Fragments arranged by size: 1263 950 236 235 85 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 712 854 1436 1627 1654 2023 2527 2539 Size: 712 142 582 191 27 369 504 12 Cuts at: 2539 2638 2722 2769 Size: 99 84 47 Fragments arranged by size: 712 582 504 369 191 142 99 84 47 27 12 Bpu10I CC'TnA_GC Cuts at: 0 523 805 2769 Size: 523 282 1964 Fragments arranged by size: 1964 523 282 BsaI GGTCTCn'nnnn_ Cuts at: 0 852 1097 2611 2769 Size: 852 245 1514 158 Fragments arranged by size: 1514 852 245 158 BsaHI Gr'CG_yC Cuts at: 0 1012 2682 2769 Size: 1012 1670 87 Fragments arranged by size: 1670 1012 87 BsaWI w'CCGG_w Cuts at: 0 458 2018 2267 2769 Size: 458 1560 249 502 Fragments arranged by size: 1560 502 458 249 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 580 610 946 976 1309 1339 2005 2035 Size: 580 30 336 30 333 30 666 30 Cuts at: 2035 2769 Size: 734 Fragments arranged by size: 734 666 580 336 333 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 77 95 371 1078 1324 1741 1789 1993 Size: 77 18 276 707 246 417 48 204 Cuts at: 1993 1996 2332 2536 2629 2764 2769 Size: 3 336 204 93 135 5 Fragments arranged by size: 707 417 336 276 246 204 204 135 93 77 48 18 5 3 BseYI C'CCAG_C Cuts at: 0 263 267 372 1047 2735 2769 Size: 263 4 105 675 1688 34 Fragments arranged by size: 1688 675 263 105 34 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 2231 2769 Size: 502 1729 538 Fragments arranged by size: 1729 538 502 BsiEI CG_ry'CG Cuts at: 0 2475 2769 Size: 2475 294 BsiHKAI G_wGCw'C Cuts at: 0 432 2102 2327 2675 2769 Size: 432 1670 225 348 94 Fragments arranged by size: 1670 432 348 225 94 BsmI GAATG_Cn' Cuts at: 0 138 330 2769 Size: 138 192 2439 Fragments arranged by size: 2439 192 138 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1003 2769 Size: 1003 1766 Bsp1286I G_dGCh'C Cuts at: 0 85 320 432 556 632 890 1793 1819 Size: 85 235 112 124 76 258 903 26 Cuts at: 1819 2102 2327 2675 2769 Size: 283 225 348 94 Fragments arranged by size: 903 348 283 258 235 225 124 112 94 85 76 26 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 668 1122 1238 1283 1527 2351 2769 Size: 668 454 116 45 244 824 418 Fragments arranged by size: 824 668 454 418 244 116 45 BsrBI CCG'CTC Cuts at: 0 1190 2769 Size: 1190 1579 BsrFI r'CCGG_y Cuts at: 0 2162 2474 2754 2769 Size: 2162 312 280 15 Fragments arranged by size: 2162 312 280 15 BssHII G'CGCG_C Cuts at: 0 2451 2769 Size: 2451 318 BstAPI GCAn_nnn'nTGC Cuts at: 0 660 1405 2589 2769 Size: 660 745 1184 180 Fragments arranged by size: 1184 745 660 180 BstBI TT'CG_AA Cuts at: 0 922 2769 Size: 922 1847 BstEII G'GTnAC_C Cuts at: 0 2244 2769 Size: 2244 525 BstXI CCAn_nnnn'nTGG Cuts at: 0 2082 2229 2769 Size: 2082 147 540 Fragments arranged by size: 2082 540 147 BstYI r'GATC_y Cuts at: 0 419 839 1595 2085 2180 2198 2495 2705 Size: 419 420 756 490 95 18 297 210 Cuts at: 2705 2769 Size: 64 Fragments arranged by size: 756 490 420 419 297 210 95 64 18 BtgI C'CryG_G Cuts at: 0 1560 2769 Size: 1560 1209 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1512 2769 Size: 1512 1257 BtsI GCAGTG_nn' Cuts at: 0 320 514 893 1076 2769 Size: 320 194 379 183 1693 Fragments arranged by size: 1693 379 320 194 183 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2130 2165 2769 Size: 2130 35 604 Fragments arranged by size: 2130 604 35 EaeI y'GGCC_r Cuts at: 0 113 1619 1733 2472 2769 Size: 113 1506 114 739 297 Fragments arranged by size: 1506 739 297 114 113 EagI C'GGCC_G Cuts at: 0 2472 2769 Size: 2472 297 EarI CTCTTCn'nnn_ Cuts at: 0 80 215 389 502 855 945 1055 1881 Size: 80 135 174 113 353 90 110 826 Cuts at: 1881 2061 2332 2638 2769 Size: 180 271 306 131 Fragments arranged by size: 826 353 306 271 180 174 135 131 113 110 90 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2620 2647 2769 Size: 2620 27 122 Fragments arranged by size: 2620 122 27 EcoICRI GAG'CTC Cuts at: 0 430 2673 2769 Size: 430 2243 96 Fragments arranged by size: 2243 430 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 385 712 854 1048 1240 1436 1627 Size: 130 255 327 142 194 192 196 191 Cuts at: 1627 1654 1864 2023 2047 2260 2527 2533 2539 Size: 27 210 159 24 213 267 6 6 Cuts at: 2539 2570 2638 2722 2769 Size: 31 68 84 47 Fragments arranged by size: 327 267 255 213 210 196 194 192 191 159 142 130 84 68 47 31 27 24 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 2314 2769 Size: 2314 455 EcoO109I rG'GnC_Cy Cuts at: 0 82 552 553 1240 1320 1497 1815 1836 Size: 82 470 1 687 80 177 318 21 Cuts at: 1836 1923 2295 2769 Size: 87 372 474 Fragments arranged by size: 687 474 470 372 318 177 87 82 80 21 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1616 2769 Size: 1616 1153 FspI TGC'GCA Cuts at: 0 1163 2769 Size: 1163 1606 HaeII r_GCGC'y Cuts at: 0 18 735 1212 2769 Size: 18 717 477 1557 Fragments arranged by size: 1557 717 477 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 580 612 754 786 2005 2037 2769 Size: 580 32 142 32 1219 32 732 Fragments arranged by size: 1219 732 580 142 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 374 548 932 2769 Size: 374 174 384 1837 Fragments arranged by size: 1837 384 374 174 MscI TGG'CCA Cuts at: 0 115 2769 Size: 115 2654 MspA1I CmG'CkG Cuts at: 0 21 165 267 615 1157 1842 2229 2471 Size: 21 144 102 348 542 685 387 242 Cuts at: 2471 2658 2700 2712 2735 2769 Size: 187 42 12 23 34 Fragments arranged by size: 685 542 387 348 242 187 144 102 42 34 23 21 12 NaeI GCC'GGC Cuts at: 0 2476 2756 2769 Size: 2476 280 13 Fragments arranged by size: 2476 280 13 NcoI C'CATG_G Cuts at: 0 1560 2769 Size: 1560 1209 NgoMIV G'CCGG_C Cuts at: 0 2474 2754 2769 Size: 2474 280 15 Fragments arranged by size: 2474 280 15 NspI r_CATG'y Cuts at: 0 394 978 2073 2769 Size: 394 584 1095 696 Fragments arranged by size: 1095 696 584 394 PasI CC'CwG_GG Cuts at: 0 402 632 2769 Size: 402 230 2137 Fragments arranged by size: 2137 402 230 PflMI CCAn_nnn'nTGG Cuts at: 0 877 1205 2505 2715 2769 Size: 877 328 1300 210 54 Fragments arranged by size: 1300 877 328 210 54 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2035 2067 2769 Size: 2035 32 702 Fragments arranged by size: 2035 702 32 PpuMI rG'GwC_Cy Cuts at: 0 1240 1923 2769 Size: 1240 683 846 Fragments arranged by size: 1240 846 683 PsiI TTA'TAA Cuts at: 0 1670 2769 Size: 1670 1099 PspOMI G'GGCC_C Cuts at: 0 81 552 1815 2769 Size: 81 471 1263 954 Fragments arranged by size: 1263 954 471 81 PstI C_TGCA'G Cuts at: 0 900 1235 1344 2450 2669 2699 2769 Size: 900 335 109 1106 219 30 70 Fragments arranged by size: 1106 900 335 219 109 70 30 PvuII CAG'CTG Cuts at: 0 267 2229 2700 2735 2769 Size: 267 1962 471 35 34 Fragments arranged by size: 1962 471 267 35 34 RsrII CG'GwC_CG Cuts at: 0 2021 2769 Size: 2021 748 SacI G_AGCT'C Cuts at: 0 432 2675 2769 Size: 432 2243 94 Fragments arranged by size: 2243 432 94 SanDI GG'GwC_CC Cuts at: 0 1240 2769 Size: 1240 1529 SapI GCTCTTCn'nnn_ Cuts at: 0 2332 2638 2769 Size: 2332 306 131 Fragments arranged by size: 2332 306 131 SfcI C'TryA_G Cuts at: 0 896 1231 1340 1659 1823 2446 2665 2695 Size: 896 335 109 319 164 623 219 30 Cuts at: 2695 2769 Size: 74 Fragments arranged by size: 896 623 335 319 219 164 109 74 30 SgrAI Cr'CCGG_yG Cuts at: 0 2162 2769 Size: 2162 607 SmaI CCC'GGG Cuts at: 0 550 947 1906 2769 Size: 550 397 959 863 Fragments arranged by size: 959 863 550 397 SphI G_CATG'C Cuts at: 0 978 2769 Size: 978 1791 StyI C'CwwG_G Cuts at: 0 649 1003 1409 1560 1736 1751 1793 2075 Size: 649 354 406 151 176 15 42 282 Cuts at: 2075 2193 2402 2769 Size: 118 209 367 Fragments arranged by size: 649 406 367 354 282 209 176 151 118 42 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 259 2727 2769 Size: 259 2468 42 Fragments arranged by size: 2468 259 42 TatI w'GTAC_w Cuts at: 0 2279 2769 Size: 2279 490 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1309 1341 2769 Size: 1309 32 1428 Fragments arranged by size: 1428 1309 32 XbaI T'CTAG_A Cuts at: 0 1432 2769 Size: 1432 1337 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1651 2565 2769 Size: 1651 914 204 Fragments arranged by size: 1651 914 204 XmaI C'CCGG_G Cuts at: 0 548 945 1904 2769 Size: 548 397 959 865 Fragments arranged by size: 959 865 548 397 Enzymes that do cut: AarI AcuI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbsI BcgI BglI BlpI Bme1580I BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I MmeI MscI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PpiI PpuMI PsiI PspOMI PstI PvuII RsrII SacI SanDI SapI SfcI SgrAI SmaI SphI StyI TaqII TatI TstI XbaI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsiWI BspEI BspHI BsrDI BsrGI BssSI BstZ17I Bsu36I ClaI DraI DraIII DrdI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MslI NarI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PshAI PspXI PsrI PvuI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XhoI XmnI ZraI