(Circular) (Six-base) MAPSORT of: pFN21AB8431.seq Check: 9759 from: 1 to: 6592 pFN21AB8431 6592 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4402 278 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 3935 1937 Size: 1284 714 4594 Fragments arranged by size: 4594 1284 714 Acc65I G'GTAC_C Cuts at: 3913 3913 Size: 6592 AclI AA'CG_TT Cuts at: 1563 4719 5092 1563 Size: 3156 373 3063 Fragments arranged by size: 3156 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2043 2115 2180 4655 6039 6307 1632 Size: 411 72 65 2475 1384 268 1917 Fragments arranged by size: 2475 1917 1384 411 268 72 65 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6395 AflIII A'CryG_T Cuts at: 1203 3829 5491 6468 1203 Size: 2626 1662 977 1327 Fragments arranged by size: 2626 1662 1327 977 AgeI A'CCGG_T Cuts at: 5423 5423 Size: 6592 AhdI GACnn_n'nnGTC Cuts at: 5321 5321 Size: 6592 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3553 1184 Size: 2369 4223 AlwNI CAG_nnn'CTG Cuts at: 4335 5907 4335 Size: 1572 5020 ApaLI G'TGCA_C Cuts at: 4649 5805 4649 Size: 1156 5436 ApoI r'AATT_y Cuts at: 1493 2081 2266 2390 2446 2474 2797 3093 3212 Size: 588 185 124 56 28 323 296 119 Cuts at: 3212 3355 3901 4127 4279 6388 1493 Size: 143 546 226 152 2109 1697 Fragments arranged by size: 2109 1697 588 546 323 296 226 185 152 143 124 119 56 28 AseI AT'TA_AT Cuts at: 160 1032 2232 5146 160 Size: 872 1200 2914 1606 Fragments arranged by size: 2914 1606 1200 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 AvaI C'yCGr_G Cuts at: 1870 1943 2421 3917 1870 Size: 73 478 1496 4545 Fragments arranged by size: 4545 1496 478 73 BamHI G'GATC_C Cuts at: 3922 4289 3922 Size: 367 6225 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3913 5368 618 Size: 325 217 627 2126 1455 1842 Fragments arranged by size: 2126 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3911 1391 Size: 2520 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2134 3260 928 Size: 971 235 1126 4260 Fragments arranged by size: 4260 1126 971 235 BbvCI CC'TCA_GC Cuts at: 3131 3131 Size: 6592 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4783 4817 1903 Size: 34 2846 34 3678 Fragments arranged by size: 3678 2846 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4484 5694 4484 Size: 1210 5382 BclI T'GATC_A Cuts at: 1548 1548 Size: 6592 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5203 136 Size: 107 122 71 631 605 3531 1525 Fragments arranged by size: 3531 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6587 6587 Size: 6592 BlpI GC'TnA_GC Cuts at: 4009 4009 Size: 6592 Bme1580I G_kGCm'C Cuts at: 1790 4653 5809 1790 Size: 2863 1156 2573 Fragments arranged by size: 2863 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6592 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4323 5281 442 Size: 416 53 83 399 24 2906 958 1753 Fragments arranged by size: 2906 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6592 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2023 3509 5252 1239 Size: 784 1486 1743 2579 Fragments arranged by size: 2579 1743 1486 784 Bpu10I CC'TnA_GC Cuts at: 3131 3131 Size: 6592 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2513 2981 3608 4717 5582 5880 2513 Size: 468 627 1109 865 298 3225 Fragments arranged by size: 3225 1109 865 627 468 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5255 882 Size: 617 3756 2219 Fragments arranged by size: 3756 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4383 493 Size: 711 3179 2702 Fragments arranged by size: 3179 2702 711 BsaBI GATnn'nnATC Cuts at: 3405 6586 3405 Size: 3181 3411 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4399 4781 5416 275 Size: 53 83 186 936 2866 382 635 1451 Fragments arranged by size: 2866 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5025 5423 5697 5844 6255 5025 Size: 398 274 147 411 5362 Fragments arranged by size: 5362 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6367 Fragments arranged by size: 6367 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2238 1186 Size: 1052 5540 BseYI C'CCAG_C Cuts at: 5795 6291 5795 Size: 496 6096 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6019 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4042 4803 5831 1540 Size: 275 186 2041 761 1028 2301 Fragments arranged by size: 2301 2041 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4653 4738 5809 4653 Size: 85 1071 5436 Fragments arranged by size: 5436 1071 85 BsmI GAATG_Cn' Cuts at: 4103 4196 4103 Size: 93 6499 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3911 4653 4738 5809 1391 Size: 399 2121 742 85 1071 2174 Fragments arranged by size: 2174 2121 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6255 6255 Size: 6592 BspHI T'CATG_A Cuts at: 4481 6211 4481 Size: 1730 4862 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3947 844 Size: 3103 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4479 1394 Size: 429 2656 3507 Fragments arranged by size: 3507 2656 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2528 3748 5087 5261 65 Size: 1183 1280 1220 1339 174 1396 Fragments arranged by size: 1396 1339 1280 1220 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5236 5423 1700 Size: 110 3426 187 2869 Fragments arranged by size: 3426 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6592 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6592 BssSI C'ACGA_G Cuts at: 4652 5664 4652 Size: 1012 5580 BstAPI GCAn_nnn'nTGC Cuts at: 794 2821 794 Size: 2027 4565 BstBI TT'CG_AA Cuts at: 5396 5396 Size: 6592 BstEII G'GTnAC_C Cuts at: 1184 2768 1184 Size: 1584 5008 BstXI CCAn_nnnn'nTGG Cuts at: 2964 3637 2964 Size: 673 5919 BstYI r'GATC_y Cuts at: 1972 3436 3922 4289 4674 4691 6143 6229 6241 Size: 1464 486 367 385 17 1452 86 12 Cuts at: 6241 6587 1972 Size: 346 1977 Fragments arranged by size: 1977 1464 1452 486 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2754 2904 513 Size: 552 939 750 150 4201 Fragments arranged by size: 4201 939 750 552 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2040 487 Size: 683 56 814 5039 Fragments arranged by size: 5039 814 683 56 BtsI GCAGTG_nn' Cuts at: 4108 4902 4922 4108 Size: 794 20 5778 Fragments arranged by size: 5778 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6592 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6557 DraI TTT'AAA Cuts at: 3896 3896 Size: 6592 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5599 809 Size: 507 4283 1802 Fragments arranged by size: 4283 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4039 4928 6557 Size: 54 1425 50 275 18 2209 889 1629 Cuts at: 6557 8 Size: 43 Fragments arranged by size: 2209 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4039 1537 Size: 275 2227 4090 Fragments arranged by size: 4090 2227 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2091 2199 4522 1353 Size: 738 108 2323 3423 Fragments arranged by size: 3423 2323 738 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5179 5553 5699 387 Size: 1064 138 3590 374 146 1280 Fragments arranged by size: 3590 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2023 2043 2115 2180 3509 4655 5252 Size: 393 391 20 72 65 1329 1146 597 Cuts at: 5252 6039 6307 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1329 1146 787 597 393 391 268 72 65 20 EcoNI CCTnn'n_nnAGG Cuts at: 3206 3206 Size: 6592 EcoO109I rG'GnC_Cy Cuts at: 2601 2614 3086 3803 2601 Size: 13 472 717 5390 Fragments arranged by size: 5390 717 472 13 EcoRI G'AATT_C Cuts at: 3901 4279 3901 Size: 378 6214 EcoRV GAT'ATC Cuts at: 3523 3523 Size: 6592 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2198 3185 2198 Size: 987 5605 FspI TGC'GCA Cuts at: 5098 5098 Size: 6592 HaeII r_GCGC'y Cuts at: 1678 5739 1678 Size: 4061 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5233 5265 5307 5339 1373 Size: 32 135 32 3661 32 42 32 2626 Fragments arranged by size: 3661 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2557 2787 3495 3827 3936 4182 Size: 988 281 619 230 708 332 109 246 Cuts at: 4182 669 Size: 3079 Fragments arranged by size: 3079 988 708 619 332 281 246 230 109 HindIII A'AGCT_T Cuts at: 748 748 Size: 6592 HpaI GTT'AAC Cuts at: 2787 4182 2787 Size: 1395 5197 KpnI G_GTAC'C Cuts at: 3917 3917 Size: 6592 MfeI C'AATT_G Cuts at: 4191 4191 Size: 6592 MluI A'CGCG_T Cuts at: 6468 6468 Size: 6592 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5706 5890 1305 Size: 4401 184 2007 Fragments arranged by size: 4401 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4770 Fragments arranged by size: 4770 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4550 4909 5068 518 Size: 648 3384 359 159 2042 Fragments arranged by size: 3384 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4008 4685 5833 6078 1606 Size: 2402 677 1148 245 2120 Fragments arranged by size: 2402 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2754 2904 513 Size: 552 939 750 150 4201 Fragments arranged by size: 4201 939 750 552 150 NdeI CA'TA_TG Cuts at: 387 3564 387 Size: 3177 3415 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6592 NotI GC'GGCC_GC Cuts at: 4039 4039 Size: 6592 NspI r_CATG'y Cuts at: 1132 1229 2204 2824 3507 3576 3833 3950 5495 Size: 97 975 620 683 69 257 117 1545 Cuts at: 5495 1132 Size: 2229 Fragments arranged by size: 2229 1545 975 683 620 257 117 97 69 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6592 PciI A'CATG_T Cuts at: 3829 5491 3829 Size: 1662 4930 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6592 PmeI GTTT'AAAC Cuts at: 3896 3896 Size: 6592 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4661 4693 6174 6206 4661 Size: 32 1481 32 5047 Fragments arranged by size: 5047 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2601 2614 3086 3803 2601 Size: 13 472 717 5390 Fragments arranged by size: 5390 717 472 13 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6592 PsiI TTA'TAA Cuts at: 2388 4162 2388 Size: 1774 4818 PstI C_TGCA'G Cuts at: 830 3350 3944 830 Size: 2520 594 3478 Fragments arranged by size: 3478 2520 594 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6592 SalI G'TCGA_C Cuts at: 1936 3934 1936 Size: 1998 4594 SanDI GG'GwC_CC Cuts at: 2601 2614 3803 2601 Size: 13 1189 5390 Fragments arranged by size: 5390 1189 13 SapI GCTCTTCn'nnn_ Cuts at: 2199 2199 Size: 6592 SbfI CC_TGCA'GG Cuts at: 3944 3944 Size: 6592 ScaI AGT'ACT Cuts at: 4840 6552 4840 Size: 1712 4880 SfcI C'TryA_G Cuts at: 826 1045 3023 3346 3940 5075 5756 5947 826 Size: 219 1978 323 594 1135 681 191 1471 Fragments arranged by size: 1978 1471 1135 681 594 323 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6592 SmaI CCC'GGG Cuts at: 1872 3919 1872 Size: 2047 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2421 2528 2996 3587 4696 5597 Size: 197 926 478 107 468 591 1109 901 Cuts at: 5597 5859 820 Size: 262 1553 Fragments arranged by size: 1553 1109 926 901 591 478 468 262 197 107 SnaBI TAC'GTA Cuts at: 493 4383 493 Size: 3890 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6592 SphI G_CATG'C Cuts at: 1132 2204 3950 1132 Size: 1072 1746 3774 Fragments arranged by size: 3774 1746 1072 SspI AAT'ATT Cuts at: 5 52 2106 4516 5 Size: 47 2054 2410 2081 Fragments arranged by size: 2410 2081 2054 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6592 StyI C'CwwG_G Cuts at: 513 1065 2004 2754 2904 2963 4031 513 Size: 552 939 750 150 59 1068 3074 Fragments arranged by size: 3074 1068 939 750 552 150 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4619 4636 4789 4974 5419 325 Size: 1255 3039 17 153 185 445 1498 Fragments arranged by size: 3039 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2877 4838 6550 Size: 274 80 33 51 1443 900 1961 1712 Cuts at: 6550 96 Size: 138 Fragments arranged by size: 1961 1712 1443 900 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6552 6584 6552 Size: 32 6560 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6592 XbaI T'CTAG_A Cuts at: 3928 3928 Size: 6592 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2446 3814 2446 Size: 1368 5224 XhoI C'TCGA_G Cuts at: 1943 2421 1943 Size: 478 6114 XmaI C'CCGG_G Cuts at: 1870 3917 1870 Size: 2047 4545 XmnI GAAnn'nnTTC Cuts at: 4721 4721 Size: 6592 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4400 276 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI