Restriction map for
pFN21AE9501
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII AccIII AclI AcyI AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BbrPI BciVI BclI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsmBI BspDI BspEI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoT22I FauI FbaI FseI FspI
HaeII Hin1I HindIII HpaI KasI
KspI MaeII MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 401 GGTACC G^GTACC
AccI 889 GTAGAC GT^[AC][GT]AC
AccII 1225 CGCG CG^CG
AcsI 1442 GAATTC [GA]^AATT[CT]
AflII 406 CTTAAG C^TTAAG
AflIII 1349 ACATGT A^C[GA][CT]GT
Alw44I 922 GTGCAC G^TGCAC
AlwNI 1006 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 396 CCTCAGG CC^T[ACGT]AGG
ApaLI 922 GTGCAC G^TGCAC
ApoI 1442 GAATTC [GA]^AATT[CT]
Asp718I 401 GGTACC G^GTACC
AvaI 425 CCCGAG C^[CT]CG[GA]G
BamHI 1283 GGATCC G^GATCC
BbvCI 714 GCTGAGG CCTCAGC(-5/-2)
BcgI 652 CGACAACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 669 CCGGG CC^[GC]GG
BfaI 487 CTAG C^TAG
BfrI 406 CTTAAG C^TTAAG
BglII 635 AGATCT A^GATCT
BsaWI 974 ACCGGA [AT]^CCGG[AT]
BseRI 1128 GAGGAG GAGGAG(10/8)
BsmI 1188 GCATTC GAATGC(1/-1)
BsoBI 425 CCCGAG C^[CT]CG[GA]G
Bsp1407I 191 TGTACA T^GTACA
BspHI 575 TCATGA T^CATGA
BspLU11I 1349 ACATGT A^CATGT
BsrGI 191 TGTACA T^GTACA
BstAPI 104 GCACTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1225 CGCG CG^CG
Bsu36I 396 CCTCAGG CC^T[ACGT]AGG
CfoI 522 GCGC GCG^C
DraIII 175 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1173 GAAGAG CTCTTC(1/4)
Eco81I 396 CCTCAGG CC^T[ACGT]AGG
EcoRI 1442 GAATTC G^AATTC
EcoRV 1035 GATATC GAT^ATC
HgaI 337 GCGTC GACGC(5/10)
HhaI 522 GCGC GCG^C
HinP1I 522 GCGC G^CGC
HincII 970 GTCAAC GT[CT]^[GA]AC
HindII 970 GTCAAC GT[CT]^[GA]AC
KpnI 401 GGTACC GGTAC^C
Ksp632I 1173 GAAGAG CTCTTC(1/4)
MaeI 487 CTAG C^TAG
MslI 240 CATGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 76 CAGCTG C[AC]G^C[GT]G
MvnI 1225 CGCG CG^CG
NciI 669 CCGGG CC^[GC]GG
PflMI 174 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 685 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 76 CAGCTG CAG^CTG
RcaI 575 TCATGA T^CATGA
SacI 673 GAGCTC GAGCT^C
SexAI 562 ACCTGGT A^CC[AT]GGT
SspBI 191 TGTACA T^GTACA
StuI 1146 AGGCCT AGG^CCT
TaqI 1077 TCGA T^CGA
TfiI 495 GAATC G^A[AT]TC
TthHB8I 1077 TCGA T^CGA
Van91I 174 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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Asp700I 495 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1428 GAAGACATTC
AvaII 96 GGTCC G^G[AT]CC
686 GGTCC
BanI 229 GGTGCC G^G[CT][GA]CC
401 GGTACC
BanII 127 GGGCTC G[GA]GC[CT]^C
673 GAGCTC
BsiWI 153 CGTACG C^GTACG
648 CGTACG
BsmFI 956 GGGAC GGGAC(10/14)
687 GTCCC
BsrI 282 ACTGG ACTGG(1/-1)
856 CCAGT
DsaI 314 CCATGG C^C[GA][CT]GG
981 CCATGG
HapII 669 CCGG C^CGG
975 CCGG
HpaII 669 CCGG C^CGG
975 CCGG
MseI 407 TTAA T^TAA
710 TTAA
MspI 669 CCGG C^CGG
975 CCGG
NcoI 314 CCATGG C^CATGG
981 CCATGG
NspI 926 ACATGC [GA]CATG^[CT]
1349 ACATGT
PleI 1084 GACTC GAGTC(4/5)
760 GAGTC
SmlI 406 CTTAAG C^T[CT][GA]AG
991 CTCAAG
Tru9I 407 TTAA T^TAA
710 TTAA
XcmI 272 CCAAGTGGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
314 CCATGGACTATGTGG
XmnI 495 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1428 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BalI BbsI BbvI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrBI BsrDI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MflI MluNI MnlI
MscI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I Tsp509I
TspRI XhoII