(Circular) (Six-base) MAPSORT of: pFN21AE9501.seq Check: 2520 from: 1 to: 6166 pFN21AE9501 6166 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3976 278 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2895 3509 1937 Size: 958 614 4594 Fragments arranged by size: 4594 958 614 Acc65I G'GTAC_C Cuts at: 2406 3487 2406 Size: 1081 5085 AclI AA'CG_TT Cuts at: 1563 4293 4666 1563 Size: 2730 373 3063 Fragments arranged by size: 3063 2730 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2854 4229 5613 5881 1632 Size: 1222 1375 1384 268 1917 Fragments arranged by size: 1917 1384 1375 1222 268 AflII C'TTAA_G Cuts at: 820 1017 2411 820 Size: 197 1394 4575 Fragments arranged by size: 4575 1394 197 AflIII A'CryG_T Cuts at: 1203 3354 5065 6042 1203 Size: 2151 1711 977 1327 Fragments arranged by size: 2151 1711 1327 977 AgeI A'CCGG_T Cuts at: 4997 4997 Size: 6166 AhdI GACnn_n'nnGTC Cuts at: 4895 4895 Size: 6166 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6166 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6166 AlwNI CAG_nnn'CTG Cuts at: 3016 3909 5481 3016 Size: 893 1572 3701 Fragments arranged by size: 3701 1572 893 ApaLI G'TGCA_C Cuts at: 2927 4223 5379 2927 Size: 1296 1156 3714 Fragments arranged by size: 3714 1296 1156 ApoI r'AATT_y Cuts at: 1493 3447 3475 3701 3853 5962 1493 Size: 1954 28 226 152 2109 1697 Fragments arranged by size: 2109 1954 1697 226 152 28 AseI AT'TA_AT Cuts at: 160 1032 4720 160 Size: 872 3688 1606 Fragments arranged by size: 3688 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 AvaI C'yCGr_G Cuts at: 1870 1943 2430 3491 1870 Size: 73 487 1061 4545 Fragments arranged by size: 4545 1061 487 73 BamHI G'GATC_C Cuts at: 3288 3496 3863 3288 Size: 208 367 5591 Fragments arranged by size: 5591 367 208 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2234 2406 3487 4942 618 Size: 325 217 627 447 172 1081 1455 1842 Fragments arranged by size: 1842 1455 1081 627 447 325 217 172 BanII G_rGCy'C Cuts at: 1391 2136 2682 3485 1391 Size: 745 546 803 4072 Fragments arranged by size: 4072 803 745 546 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2043 2350 2860 3440 928 Size: 971 144 307 510 580 3654 Fragments arranged by size: 3654 971 580 510 307 144 BbvCI CC'TCA_GC Cuts at: 2720 2720 Size: 6166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2646 2680 4357 4391 1903 Size: 34 709 34 1677 34 3678 Fragments arranged by size: 3678 1677 709 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4058 5268 4058 Size: 1210 4956 BclI T'GATC_A Cuts at: 1548 1548 Size: 6166 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4777 136 Size: 107 122 71 631 605 3105 1525 Fragments arranged by size: 3105 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2640 6161 2640 Size: 3521 2645 BlpI GC'TnA_GC Cuts at: 3583 3583 Size: 6166 Bme1580I G_kGCm'C Cuts at: 1790 2931 4227 5383 1790 Size: 1141 1296 1156 2573 Fragments arranged by size: 2573 1296 1156 1141 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6166 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2297 2855 3897 Size: 416 53 83 399 24 880 558 1042 Cuts at: 3897 4855 442 Size: 958 1753 Fragments arranged by size: 1753 1042 958 880 558 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4826 1239 Size: 3587 2579 Bpu10I CC'TnA_GC Cuts at: 2105 2720 2105 Size: 615 5551 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2981 4291 5156 5454 2981 Size: 1310 865 298 3693 Fragments arranged by size: 3693 1310 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4829 882 Size: 617 3330 2219 Fragments arranged by size: 3330 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3957 493 Size: 711 2753 2702 Fragments arranged by size: 2753 2702 711 BsaBI GATnn'nnATC Cuts at: 6160 6160 Size: 6166 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3973 4355 4990 275 Size: 53 83 186 936 2440 382 635 1451 Fragments arranged by size: 2440 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2979 4599 4997 5271 5418 5829 2979 Size: 1620 398 274 147 411 3316 Fragments arranged by size: 3316 1620 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3368 3398 1178 Size: 30 165 30 1965 30 3946 Fragments arranged by size: 3946 1965 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3148 1186 Size: 1962 4204 BseYI C'CCAG_C Cuts at: 2079 3370 5369 5865 2079 Size: 1291 1999 496 2380 Fragments arranged by size: 2380 1999 1291 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5593 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3616 4377 5405 1540 Size: 275 186 1615 761 1028 2301 Fragments arranged by size: 2301 1615 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2111 2682 2931 4227 4312 5383 2111 Size: 571 249 1296 85 1071 2894 Fragments arranged by size: 2894 1296 1071 571 249 85 BsiWI C'GTAC_G Cuts at: 2158 2653 2158 Size: 495 5671 BsmI GAATG_Cn' Cuts at: 3193 3677 3770 3193 Size: 484 93 5589 Fragments arranged by size: 5589 484 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2111 2136 2682 2931 3485 4227 4312 Size: 399 321 25 546 249 554 742 85 Cuts at: 4312 5383 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 554 546 399 321 249 85 25 BspEI T'CCGG_A Cuts at: 5829 5829 Size: 6166 BspHI T'CATG_A Cuts at: 2580 4055 5785 2580 Size: 1475 1730 2961 Fragments arranged by size: 2961 1730 1475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2148 2274 2613 3521 844 Size: 1304 126 339 908 3489 Fragments arranged by size: 3489 1304 908 339 126 BsrBI CCG'CTC Cuts at: 1394 1823 2170 2385 3189 4053 1394 Size: 429 347 215 804 864 3507 Fragments arranged by size: 3507 864 804 429 347 215 BsrDI GCAATG_nn' Cuts at: 65 1248 3315 3346 3424 4661 4835 65 Size: 1183 2067 31 78 1237 174 1396 Fragments arranged by size: 2067 1396 1237 1183 174 78 31 BsrFI r'CCGG_y Cuts at: 1700 1810 4810 4997 1700 Size: 110 3000 187 2869 Fragments arranged by size: 3000 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2196 96 Size: 2100 4066 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6166 BssSI C'ACGA_G Cuts at: 4226 5238 4226 Size: 1012 5154 BstAPI GCAn_nnn'nTGC Cuts at: 794 2115 794 Size: 1321 4845 BstBI TT'CG_AA Cuts at: 4970 4970 Size: 6166 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6166 BstXI CCAn_nnnn'nTGG Cuts at: 2449 3253 3370 2449 Size: 804 117 5245 Fragments arranged by size: 5245 804 117 BstYI r'GATC_y Cuts at: 1972 2640 2813 3288 3496 3863 4248 4265 5717 Size: 668 173 475 208 367 385 17 1452 Cuts at: 5717 5803 5815 6161 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 668 475 385 367 346 208 173 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2402 2402 Size: 6166 BtgI C'CryG_G Cuts at: 513 1065 2319 2986 513 Size: 552 1254 667 3693 Fragments arranged by size: 3693 1254 667 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5427 Fragments arranged by size: 5427 683 56 BtsI GCAGTG_nn' Cuts at: 2662 3682 4476 4496 2662 Size: 1020 794 20 4332 Fragments arranged by size: 4332 1020 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6166 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6131 DraI TTT'AAA Cuts at: 3470 3470 Size: 6166 DraIII CAC_nnn'GTG Cuts at: 2185 2185 Size: 6166 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5173 809 Size: 507 3857 1802 Fragments arranged by size: 3857 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2331 2439 Size: 54 1425 50 275 18 240 261 108 Cuts at: 2439 2989 3613 4502 6131 8 Size: 550 624 889 1629 43 Fragments arranged by size: 1629 1425 889 624 550 275 261 240 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3613 1537 Size: 275 1801 4090 Fragments arranged by size: 4090 1801 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3173 4096 1353 Size: 1820 923 3423 Fragments arranged by size: 3423 1820 923 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2234 4753 5127 5273 387 Size: 1064 138 645 2519 374 146 1280 Fragments arranged by size: 2519 1280 1064 645 374 146 138 EcoICRI GAG'CTC Cuts at: 2680 2680 Size: 6166 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2854 4229 4826 5613 5881 1239 Size: 393 1222 1375 597 787 268 1524 Fragments arranged by size: 1524 1375 1222 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2454 2691 3013 2454 Size: 237 322 5607 Fragments arranged by size: 5607 322 237 EcoRI G'AATT_C Cuts at: 3447 3475 3853 3447 Size: 28 378 5760 Fragments arranged by size: 5760 378 28 EcoRV GAT'ATC Cuts at: 3042 3042 Size: 6166 FspI TGC'GCA Cuts at: 4672 4672 Size: 6166 HaeII r_GCGC'y Cuts at: 1678 5313 1678 Size: 3635 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2840 2872 3056 3088 4807 Size: 32 135 32 1268 32 184 32 1719 Cuts at: 4807 4839 4881 4913 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1719 1268 184 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2977 3510 3756 669 Size: 988 281 1039 533 246 3079 Fragments arranged by size: 3079 1039 988 533 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6166 HpaI GTT'AAC Cuts at: 3756 3756 Size: 6166 KpnI G_GTAC'C Cuts at: 2410 3491 2410 Size: 1081 5085 MfeI C'AATT_G Cuts at: 3765 3765 Size: 6166 MluI A'CGCG_T Cuts at: 6042 6042 Size: 6166 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5280 5464 1305 Size: 3975 184 2007 Fragments arranged by size: 3975 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2072 2333 2991 10 Size: 54 1425 343 240 261 658 3185 Fragments arranged by size: 3185 1425 658 343 261 240 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 4124 4483 4642 518 Size: 648 1083 1875 359 159 2042 Fragments arranged by size: 2042 1875 1083 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 3582 4259 5407 5652 1606 Size: 477 1499 677 1148 245 2120 Fragments arranged by size: 2120 1499 1148 677 477 245 NcoI C'CATG_G Cuts at: 513 1065 2319 2986 513 Size: 552 1254 667 3693 Fragments arranged by size: 3693 1254 667 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6166 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6166 NotI GC'GGCC_GC Cuts at: 3613 3613 Size: 6166 NspI r_CATG'y Cuts at: 1132 1229 2935 3358 3524 5069 1132 Size: 97 1706 423 166 1545 2229 Fragments arranged by size: 2229 1706 1545 423 166 97 PasI CC'CwG_GG Cuts at: 1348 2772 1348 Size: 1424 4742 PciI A'CATG_T Cuts at: 3354 5065 3354 Size: 1711 4455 PflMI CCAn_nnn'nTGG Cuts at: 1108 2185 1108 Size: 1077 5089 PmeI GTTT'AAAC Cuts at: 3470 3470 Size: 6166 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4235 4267 5748 5780 4235 Size: 32 1481 32 4621 Fragments arranged by size: 4621 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2691 2691 Size: 6166 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6166 PsiI TTA'TAA Cuts at: 3736 3736 Size: 6166 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2246 2278 2246 Size: 32 6134 PstI C_TGCA'G Cuts at: 830 3518 830 Size: 2688 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6166 PvuII CAG'CTG Cuts at: 2083 2083 Size: 6166 SacI G_AGCT'C Cuts at: 2682 2682 Size: 6166 SalI G'TCGA_C Cuts at: 1936 3508 1936 Size: 1572 4594 SbfI CC_TGCA'GG Cuts at: 3518 3518 Size: 6166 ScaI AGT'ACT Cuts at: 4414 6126 4414 Size: 1712 4454 SexAI A'CCwGG_T Cuts at: 2567 2567 Size: 6166 SfcI C'TryA_G Cuts at: 826 1045 3514 4649 5330 5521 826 Size: 219 2469 1135 681 191 1471 Fragments arranged by size: 2469 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6166 SmaI CCC'GGG Cuts at: 1872 3493 1872 Size: 1621 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 2996 4270 5171 5433 820 Size: 197 926 468 585 1274 901 262 1553 Fragments arranged by size: 1553 1274 926 901 585 468 262 197 SnaBI TAC'GTA Cuts at: 493 3957 493 Size: 3464 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6166 SphI G_CATG'C Cuts at: 1132 3524 1132 Size: 2392 3774 SspI AAT'ATT Cuts at: 5 52 4090 5 Size: 47 4038 2081 Fragments arranged by size: 4038 2081 47 StuI AGG'CCT Cuts at: 1517 3153 1517 Size: 1636 4530 StyI C'CwwG_G Cuts at: 513 1065 2319 2736 2844 2986 3605 513 Size: 552 1254 417 108 142 619 3074 Fragments arranged by size: 3074 1254 619 552 417 142 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2222 4193 4210 4363 4548 4993 325 Size: 1255 642 1971 17 153 185 445 1498 Fragments arranged by size: 1971 1498 1255 642 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2196 4412 6124 Size: 274 80 33 51 1443 219 2216 1712 Cuts at: 6124 96 Size: 138 Fragments arranged by size: 2216 1712 1443 274 219 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6126 6158 6126 Size: 32 6134 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6166 XbaI T'CTAG_A Cuts at: 3502 3502 Size: 6166 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 2326 2284 Size: 42 6124 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6166 XmaI C'CCGG_G Cuts at: 1870 3491 1870 Size: 1621 4545 XmnI GAAnn'nnTTC Cuts at: 2504 3437 4295 2504 Size: 933 858 4375 Fragments arranged by size: 4375 933 858 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3974 276 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI