Restriction map for
pFN21AE9501
(Linear) (Six-base) MAPSORT of: KIEE9501.seq Check: 147 from: 1 to: 1461
KIEE9501.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AccI GT'mk_AC
Cuts at: 0 890 1461
Size: 890 571
Acc65I G'GTAC_C
Cuts at: 0 401 1461
Size: 401 1060
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 1461
Size: 849 612
AflII C'TTAA_G
Cuts at: 0 406 1461
Size: 406 1055
AflIII A'CryG_T
Cuts at: 0 1349 1461
Size: 1349 112
AlwNI CAG_nnn'CTG
Cuts at: 0 1011 1461
Size: 1011 450
ApaLI G'TGCA_C
Cuts at: 0 922 1461
Size: 922 539
ApoI r'AATT_y
Cuts at: 0 1442 1461
Size: 1442 19
AvaI C'yCGr_G
Cuts at: 0 425 1461
Size: 425 1036
BamHI G'GATC_C
Cuts at: 0 1283 1461
Size: 1283 178
BanI G'GyrC_C
Cuts at: 0 229 401 1461
Size: 229 172 1060
Fragments arranged by size:
1060 229 172
BanII G_rGCy'C
Cuts at: 0 131 677 1461
Size: 131 546 784
Fragments arranged by size:
784 546 131
BbsI GAAGACnn'nnnn_
Cuts at: 0 38 345 855 1435 1461
Size: 38 307 510 580 26
Fragments arranged by size:
580 510 307 38 26
BbvCI CC'TCA_GC
Cuts at: 0 715 1461
Size: 715 746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 641 675 1461
Size: 641 34 786
Fragments arranged by size:
786 641 34
BglII A'GATC_T
Cuts at: 0 635 1461
Size: 635 826
Bme1580I G_kGCm'C
Cuts at: 0 926 1461
Size: 926 535
BmrI ACTGGGnnnn_n'
Cuts at: 0 292 850 1461
Size: 292 558 611
Fragments arranged by size:
611 558 292
Bpu10I CC'TnA_GC
Cuts at: 0 100 715 1461
Size: 100 615 746
Fragments arranged by size:
746 615 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1461
Size: 976 485
BsaWI w'CCGG_w
Cuts at: 0 974 1461
Size: 974 487
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1363 1393 1461
Size: 1363 30 68
Fragments arranged by size:
1363 68 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1143 1461
Size: 1143 318
BseYI C'CCAG_C
Cuts at: 0 74 1365 1461
Size: 74 1291 96
Fragments arranged by size:
1291 96 74
BsiHKAI G_wGCw'C
Cuts at: 0 106 677 926 1461
Size: 106 571 249 535
Fragments arranged by size:
571 535 249 106
BsiWI C'GTAC_G
Cuts at: 0 153 648 1461
Size: 153 495 813
Fragments arranged by size:
813 495 153
BsmI GAATG_Cn'
Cuts at: 0 1188 1461
Size: 1188 273
Bsp1286I G_dGCh'C
Cuts at: 0 106 131 677 926 1461
Size: 106 25 546 249 535
Fragments arranged by size:
546 535 249 106 25
BspHI T'CATG_A
Cuts at: 0 575 1461
Size: 575 886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 143 269 608 1461
Size: 143 126 339 853
Fragments arranged by size:
853 339 143 126
BsrBI CCG'CTC
Cuts at: 0 165 380 1184 1461
Size: 165 215 804 277
Fragments arranged by size:
804 277 215 165
BsrDI GCAATG_nn'
Cuts at: 0 1310 1341 1419 1461
Size: 1310 31 78 42
Fragments arranged by size:
1310 78 42 31
BsrGI T'GTAC_A
Cuts at: 0 191 1461
Size: 191 1270
BstAPI GCAn_nnn'nTGC
Cuts at: 0 110 1461
Size: 110 1351
BstXI CCAn_nnnn'nTGG
Cuts at: 0 444 1248 1365 1461
Size: 444 804 117 96
Fragments arranged by size:
804 444 117 96
BstYI r'GATC_y
Cuts at: 0 635 808 1283 1461
Size: 635 173 475 178
Fragments arranged by size:
635 475 178 173
Bsu36I CC'TnA_GG
Cuts at: 0 397 1461
Size: 397 1064
BtgI C'CryG_G
Cuts at: 0 314 981 1461
Size: 314 667 480
Fragments arranged by size:
667 480 314
BtsI GCAGTG_nn'
Cuts at: 0 657 1461
Size: 657 804
DraIII CAC_nnn'GTG
Cuts at: 0 180 1461
Size: 180 1281
EaeI y'GGCC_r
Cuts at: 0 65 326 434 984 1461
Size: 65 261 108 550 477
Fragments arranged by size:
550 477 261 108 65
EarI CTCTTCn'nnn_
Cuts at: 0 1168 1461
Size: 1168 293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 229 1461
Size: 229 1232
EcoICRI GAG'CTC
Cuts at: 0 675 1461
Size: 675 786
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 1461
Size: 849 612
EcoO109I rG'GnC_Cy
Cuts at: 0 449 686 1008 1461
Size: 449 237 322 453
Fragments arranged by size:
453 449 322 237
EcoRI G'AATT_C
Cuts at: 0 1442 1461
Size: 1442 19
EcoRV GAT'ATC
Cuts at: 0 1037 1461
Size: 1037 424
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 835 867 1051 1083 1461
Size: 835 32 184 32 378
Fragments arranged by size:
835 378 184 32 32
HincII GTy'rAC
Cuts at: 0 972 1461
Size: 972 489
KpnI G_GTAC'C
Cuts at: 0 405 1461
Size: 405 1056
MscI TGG'CCA
Cuts at: 0 67 328 986 1461
Size: 67 261 658 475
Fragments arranged by size:
658 475 261 67
MslI CAynn'nnrTG
Cuts at: 0 244 1461
Size: 244 1217
MspA1I CmG'CkG
Cuts at: 0 78 1461
Size: 78 1383
NcoI C'CATG_G
Cuts at: 0 314 981 1461
Size: 314 667 480
Fragments arranged by size:
667 480 314
NspI r_CATG'y
Cuts at: 0 930 1353 1461
Size: 930 423 108
Fragments arranged by size:
930 423 108
PasI CC'CwG_GG
Cuts at: 0 767 1461
Size: 767 694
PciI A'CATG_T
Cuts at: 0 1349 1461
Size: 1349 112
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 1461
Size: 180 1281
PpuMI rG'GwC_Cy
Cuts at: 0 686 1461
Size: 686 775
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 241 273 1461
Size: 241 32 1188
Fragments arranged by size:
1188 241 32
PvuII CAG'CTG
Cuts at: 0 78 1461
Size: 78 1383
SacI G_AGCT'C
Cuts at: 0 677 1461
Size: 677 784
SexAI A'CCwGG_T
Cuts at: 0 562 1461
Size: 562 899
SmlI C'TyrA_G
Cuts at: 0 406 991 1461
Size: 406 585 470
Fragments arranged by size:
585 470 406
StuI AGG'CCT
Cuts at: 0 1148 1461
Size: 1148 313
StyI C'CwwG_G
Cuts at: 0 314 731 839 981 1461
Size: 314 417 108 142 480
Fragments arranged by size:
480 417 314 142 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 217 1461
Size: 217 1244
TatI w'GTAC_w
Cuts at: 0 191 1461
Size: 191 1270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 321 1461
Size: 279 42 1140
Fragments arranged by size:
1140 279 42
XmnI GAAnn'nnTTC
Cuts at: 0 499 1432 1461
Size: 499 933 29
Fragments arranged by size:
933 499 29
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BglII
Bme1580I BmrI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI
BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
Hin4I HincII KpnI MscI MslI MspA1I NcoI NspI
PasI PciI PflMI PpuMI PsrI PvuII SacI SexAI
SmlI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI
BciVI BclI BfrBI BglI BlpI BmgBI BmtI BplI
BpmI BsaI BsaAI BsaBI BsaHI BsgI BsiEI BsmBI
BspEI BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EcoNI FalI FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XhoI XmaI ZraI