################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BbeI BbrPI BbvCI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsoBI Bsp1407I BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AciI 753 GCGG CCGC(-3/-1) AfaI 69 GTAC GT^AC AluI 210 AGCT AG^CT AlwNI 50 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 388 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 431 TGGCCA TGG^CCA BanI 505 GGCACC G^G[CT][GA]CC BanII 968 GGGCTC G[GA]GC[CT]^C BbsI 877 GTCTTC GAAGAC(2/6) BcgI 803 GCACTCAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 375 GTATCC GTATCC(6/5) BpmI 533 CTGGAG CTGGAG(16/14) BpuAI 877 GTCTTC GAAGAC(2/6) BsaI 536 GAGACC GGTCTC(1/5) BsmBI 272 GAGACG CGTCTC(1/5) BsmFI 908 GGGAC GGGAC(10/14) BspDI 356 ATCGAT AT^CGAT BspHI 972 TCATGA T^CATGA BspMI 657 GCAGGT ACCTGC(4/8) BstF5I 953 CATCC GGATG(2/0) Cac8I 42 GCTGGC GC[ACGT]^[ACGT]GC ClaI 356 ATCGAT AT^CGAT EaeI 431 TGGCCA [CT]^GGCC[GA] EarI 634 CTCTTC CTCTTC(1/4) FokI 953 CATCC GGATG(9/13) Ksp632I 634 CTCTTC CTCTTC(1/4) MluNI 431 TGGCCA TGG^CCA MscI 431 TGGCCA TGG^CCA PleI 110 GAGTC GAGTC(4/5) RcaI 972 TCATGA T^CATGA RsaI 69 GTAC GT^AC ScaI 68 AGTACT AGT^ACT SspI 175 AATATT AAT^ATT TaqI 357 TCGA T^CGA TfiI 921 GAATC G^A[AT]TC Tsp45I 835 GTGAC ^GT[GC]AC Tsp509I 369 AATT ^AATT TthHB8I 357 TCGA T^CGA XmnI 388 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Alw44I 219 GTGCAC G^TGCAC 801 GTGCAC AlwI 8 GGATC GGATC(4/5) 764 GGATC ApaLI 219 GTGCAC G^TGCAC 801 GTGCAC BbvI 46 GCAGC GCAGC(8/12) 55 GCTGC BsmI 21 GAATGC GAATGC(1/-1) 326 GCATTC BssKI 60 CCTGG ^CC[ACGT]GG 777 CCTGG BstAPI 16 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 49 GCAGAGGCTGC BstNI 60 CCTGG CC^[AT]GG 777 CCTGG EcoRII 60 CCTGG ^CC[AT]GG 777 CCTGG EcoT14I 114 CCTTGG C^C[AT][AT]GG 428 CCTTGG Fnu4HI 46 GCAGC GC^[ACGT]GC 55 GCTGC HinfI 110 GAGTC G^A[ACGT]TC 921 GAATC ItaI 46 GCAGC GC^[ACGT]GC 55 GCTGC MaeII 275 ACGT A^CGT 746 ACGT MslI 705 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 912 CATGCTGATG MvaI 60 CCTGG CC^[AT]GG 777 CCTGG NlaIV 505 GGCACC GG[ACGT]^[ACGT]CC 584 GGTTCC NspI 563 GCATGT [GA]CATG^[CT] 911 ACATGC ScrFI 60 CCTGG CC^[ACGT]GG 777 CCTGG StyI 114 CCTTGG C^C[AT][AT]GG 428 CCTTGG TseI 46 GCAGC G^C[AT]GC 55 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AspHI BmyI BsaJI BsiHKAI BsmAI Bsp1286I BsrI DdeI DpnII HaeIII MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII Sau3AI Tru9I TspRI