################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstUI BstYI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MfeI MflI MluI MroI MseI MunI MvnI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 921 GTATAC GT^[AC][GT]AC AlwI 911 GGATC GGATC(4/5) AocI 697 CCTCAGG CC^T[ACGT]AGG Aor51HI 842 AGCGCT AGC^GCT BanI 799 GGTGCC G^G[CT][GA]CC BbsI 1366 GAAGAC GAAGAC(2/6) BclI 659 TGATCA T^GATCA BlpI 91 GCTCAGC GC^T[ACGT]AGC Bpu1102I 91 GCTCAGC GC^T[ACGT]AGC BpuAI 1366 GAAGAC GAAGAC(2/6) BsaAI 1447 TACGTG [CT]AC^GT[GA] BsaBI 135 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 871 CTCCTC GAGGAG(10/8) BsgI 367 GTGCAG GTGCAG(16/14) BsrBI 267 CCGCTC CCGCTC(-3/-3) BsrI 1235 CCAGT ACTGG(1/-1) BssSI 442 CTCGTG CACGAG(-5/-1) Bst1107I 921 GTATAC GTA^TAC BstEII 1041 GGTGACC G^GT[ACGT]ACC BstPI 1041 GGTGACC G^GT[ACGT]ACC BstZ17I 921 GTATAC GTA^TAC Bsu36I 697 CCTCAGG CC^T[ACGT]AGG CelII 91 GCTCAGC GC^T[ACGT]AGC Eco47III 842 AGCGCT AGC^GCT Eco81I 697 CCTCAGG CC^T[ACGT]AGG EcoNI 627 CCTGGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1041 GGTGACC G^GT[ACGT]ACC FbaI 659 TGATCA T^GATCA HincII 17 GTCAAC GT[CT]^[GA]AC HindII 17 GTCAAC GT[CT]^[GA]AC HinfI 85 GAGTC G^A[ACGT]TC MamI 135 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 38 GCCGGC GCC^GGC NcoI 1166 CCATGG C^CATGG NdeI 897 CATATG CA^TATG NgoMIV 38 GCCGGC G^CCGGC PleI 85 GAGTC GAGTC(4/5) PstI 225 CTGCAG CTGCA^G PvuII 1225 CAGCTG CAG^CTG SfcI 225 CTGCAG C^T[GA][CT]AG TaqI 1351 TCGA T^CGA TthHB8I 1351 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 113 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1225 CAGCTGCTG ApaI 200 GGGCCC GGGCC^C 583 GGGCCC AspHI 331 GTGCTC G[AT]GC[AT]^C 868 GTGCTC AspI 757 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1044 GACCAAGTC BanII 200 GGGCCC G[GA]GC[CT]^C 583 GGGCCC BpmI 98 CTGGAG CTGGAG(16/14) 292 CTGGAG BsaI 1108 GAGACC GGTCTC(1/5) 1032 GGTCTC BsiHKAI 331 GTGCTC G[AT]GC[AT]^C 868 GTGCTC BsmAI 1108 GAGAC GTCTC(1/5) 1033 GTCTC BspLU11I 1079 ACATGT A^CATGT 1370 ACATGT HaeII 491 GGCGCT [GA]GCGC^[CT] 842 AGCGCT MspA1I 1209 CAGCGG C[AC]G^C[GT]G 1225 CAGCTG PflFI 757 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1044 GACCAAGTC SfaNI 1266 GATGC GCATC(5/9) 284 GCATC SmaI 587 CCCGGG CCC^GGG 1091 CCCGGG StuI 234 AGGCCT AGG^CCT 1157 AGGCCT Tth111I 757 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1044 GACCAAGTC XmaCI 587 CCCGGG C^CCGGG 1091 CCCGGG XmaI 587 CCCGGG C^CCGGG 1091 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AfaI AflIII AluI AvaI AvaII BalI BbvI BcnI BglI BmyI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PpuMI RsaI Sau3AI Sau96I ScrFI StyI TseI Tsp45I TspRI Van91I XcmI