Restriction map for
pFN21AE9462
(Circular) (Six-base) MAPSORT of: pFN21AE9462.seq Check: 9914 from: 1 to: 6163
pFN21AE9462 6163 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2927 3506 1937
Size: 990 579 4594
Fragments arranged by size:
4594 990 579
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3390 4226 5610 5878 1632
Size: 1758 836 1384 268 1917
Fragments arranged by size:
1917 1758 1384 836 268
AfeI AGC'GCT
Cuts at: 2849 2849
Size: 6163
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5966
AflIII A'CryG_T
Cuts at: 1203 3084 3375 3453 5062 6039 1203
Size: 1881 291 78 1609 977 1327
Fragments arranged by size:
1881 1609 1327 977 291 78
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 4892 4892
Size: 6163
AleI CACnn'nnGTG
Cuts at: 1166 2333 1166
Size: 1167 4996
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AlwNI CAG_nnn'CTG
Cuts at: 2123 3235 3906 5478 2123
Size: 1112 671 1572 2808
Fragments arranged by size:
2808 1572 1112 671
ApaI G_GGCC'C
Cuts at: 2209 2592 2209
Size: 383 5780
ApaLI G'TGCA_C
Cuts at: 4220 5376 4220
Size: 1156 5007
ApoI r'AATT_y
Cuts at: 1493 3472 3698 3850 5959 1493
Size: 1979 226 152 2109 1697
Fragments arranged by size:
2109 1979 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 2592 3032 3096 3488 1870
Size: 73 649 440 64 392 4545
Fragments arranged by size:
4545 649 440 392 73 64
BamHI G'GATC_C
Cuts at: 3493 3860 3493
Size: 367 5796
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2804 3484 4939 618
Size: 325 217 627 1017 680 1455 1842
Fragments arranged by size:
1842 1455 1017 680 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2209 2592 3482 1391
Size: 818 383 890 4072
Fragments arranged by size:
4072 890 818 383
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3378 928
Size: 971 1479 3713
Fragments arranged by size:
3713 1479 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4354 4388 1903
Size: 34 50 34 2333 34 3678
Fragments arranged by size:
3678 2333 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5265 4055
Size: 1210 4953
BclI T'GATC_A
Cuts at: 1548 2664 1548
Size: 1116 5047
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2141 2706
Size: 107 122 71 631 605 377 92 565
Cuts at: 2706 4774 136
Size: 2068 1525
Fragments arranged by size:
2068 1525 631 605 565 377 122 107
92 71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 2097 3580 2097
Size: 1483 4680
Bme1580I G_kGCm'C
Cuts at: 1790 2209 2592 4224 5380 1790
Size: 419 383 1632 1156 2573
Fragments arranged by size:
2573 1632 1156 419 383
BmgBI CAC'GTC
Cuts at: 1318 2551 1318
Size: 1233 4930
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3894 4852 442
Size: 416 53 83 399 24 2477 958 1753
Fragments arranged by size:
2477 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2124 2318 4823 1239
Size: 885 194 2505 2579
Fragments arranged by size:
2579 2505 885 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4288 5153 5451 4288
Size: 865 298 5000
Fragments arranged by size:
5000 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3043 3107 4826 882
Size: 617 1544 64 1719 2219
Fragments arranged by size:
2219 1719 1544 617 64
BsaAI yAC'GTr
Cuts at: 493 1204 3454 3954 493
Size: 711 2250 500 2702
Fragments arranged by size:
2702 2250 711 500
BsaBI GATnn'nnATC
Cuts at: 2144 6157 2144
Size: 4013 2150
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3970 4352 4987 275
Size: 53 83 186 936 2437 382 635 1451
Fragments arranged by size:
2437 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4596 4994 5268 5415 5826 4596
Size: 398 274 147 411 4933
Fragments arranged by size:
4933 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5938
Fragments arranged by size:
5938 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2867 1186
Size: 1681 4482
BseYI C'CCAG_C
Cuts at: 2309 2398 2598 2724 2800 5366 5862 2309
Size: 89 200 126 76 2566 496 2610
Fragments arranged by size:
2610 2566 496 200 126 89 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2393 1163
Size: 573 657 4933
Fragments arranged by size:
4933 657 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3613 4374 5402 1540
Size: 275 186 1612 761 1028 2301
Fragments arranged by size:
2301 1612 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 2877 4224 4309 5380 2340
Size: 537 1347 85 1071 3123
Fragments arranged by size:
3123 1347 1071 537 85
BsmI GAATG_Cn'
Cuts at: 3674 3767 3674
Size: 93 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2209 2340 2592 2877 3482 4224 4309
Size: 399 419 131 252 285 605 742 85
Cuts at: 4309 5380 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 605 419 399 285 252
131 85
BspEI T'CCGG_A
Cuts at: 5826 5826
Size: 6163
BspHI T'CATG_A
Cuts at: 4052 5782 4052
Size: 1730 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3518 844
Size: 2674 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2274 4050 1394
Size: 429 451 1776 3507
Fragments arranged by size:
3507 1776 451 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4658 4832 65
Size: 1183 3410 174 1396
Fragments arranged by size:
3410 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2043 3181 3199 4807 4994 1700
Size: 110 233 1138 18 1608 187 2869
Fragments arranged by size:
2869 1608 1138 233 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6163
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6163
BssSI C'ACGA_G
Cuts at: 2447 4223 5235 2447
Size: 1776 1012 3375
Fragments arranged by size:
3375 1776 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6163
BstBI TT'CG_AA
Cuts at: 4967 4967
Size: 6163
BstEII G'GTnAC_C
Cuts at: 1184 3046 1184
Size: 1862 4301
BstXI CCAn_nnnn'nTGG
Cuts at: 2202 2350 2908 3262 2202
Size: 148 558 354 5103
Fragments arranged by size:
5103 558 354 148
BstYI r'GATC_y
Cuts at: 1972 3493 3860 4245 4262 5714 5800 5812 6158
Size: 1521 367 385 17 1452 86 12 346
Cuts at: 6158 1972
Size: 1977
Fragments arranged by size:
1977 1521 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2928 2928
Size: 6163
Bsu36I CC'TnA_GG
Cuts at: 2703 2703
Size: 6163
BtgI C'CryG_G
Cuts at: 513 1065 2304 2584 2730 3171 3314 513
Size: 552 1239 280 146 441 143 3362
Fragments arranged by size:
3362 1239 552 441 280 146 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2734 2968 487
Size: 683 56 1508 234 3682
Fragments arranged by size:
3682 1508 683 234 56
BtsI GCAGTG_nn'
Cuts at: 2692 3352 3679 4473 4493 2692
Size: 660 327 794 20 4362
Fragments arranged by size:
4362 794 660 327 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2878 2913 529
Size: 35 2314 35 3779
Fragments arranged by size:
3779 2314 35 35
DraI TTT'AAA
Cuts at: 3467 3467
Size: 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2355 2836
Size: 54 1425 50 275 18 312 213 481
Cuts at: 2836 2979 3402 3610 4499 6128 8
Size: 143 423 208 889 1629 43
Fragments arranged by size:
1629 1425 889 481 423 312 275 213
208 143 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3610 1537
Size: 275 1798 4090
Fragments arranged by size:
4090 1798 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2189 2444 3023 4093 1353
Size: 836 255 579 1070 3423
Fragments arranged by size:
3423 1070 836 579 255
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4750 5124 5270 387
Size: 1064 138 3161 374 146 1280
Fragments arranged by size:
3161 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2318 3390 4226 4823 5610 5878
Size: 393 492 194 1072 836 597 787 268
Cuts at: 5878 1239
Size: 1524
Fragments arranged by size:
1524 1072 836 787 597 492 393 268
194
EcoNI CCTnn'n_nnAGG
Cuts at: 2636 2636
Size: 6163
EcoO109I rG'GnC_Cy
Cuts at: 2109 2199 2403 2427 2589 2817 2109
Size: 90 204 24 162 228 5455
Fragments arranged by size:
5455 228 204 162 90 24
EcoRI G'AATT_C
Cuts at: 3472 3850 3472
Size: 378 5785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 6163
FspI TGC'GCA
Cuts at: 4669 4669
Size: 6163
HaeII r_GCGC'y
Cuts at: 1678 2500 2851 5310 1678
Size: 822 351 2459 2531
Fragments arranged by size:
2531 2459 822 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2166 2198 3365 3397 4804
Size: 32 135 32 594 32 1167 32 1407
Cuts at: 4804 4836 4878 4910 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1407 1167 594 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2024 3507 3753 669
Size: 988 281 86 1483 246 3079
Fragments arranged by size:
3079 1483 988 281 246 86
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6163
HpaI GTT'AAC
Cuts at: 3753 3753
Size: 6163
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5277 5461 1305
Size: 3972 184 2007
Fragments arranged by size:
3972 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 2357 2981 10
Size: 54 1425 343 312 213 624 3192
Fragments arranged by size:
3192 1425 624 343 312 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 2351 2906 3149 3260 4121 4480
Size: 648 1167 18 555 243 111 861 359
Cuts at: 4480 4639 518
Size: 159 2042
Fragments arranged by size:
2042 1167 861 648 555 359 243 159
111 18
MspA1I CmG'CkG
Cuts at: 1606 3216 3232 3579 4256 5404 5649 1606
Size: 1610 16 347 677 1148 245 2120
Fragments arranged by size:
2120 1610 1148 677 347 245 16
NaeI GCC'GGC
Cuts at: 2045 2045
Size: 6163
NcoI C'CATG_G
Cuts at: 513 1065 3171 513
Size: 552 2106 3505
Fragments arranged by size:
3505 2106 552
NdeI CA'TA_TG
Cuts at: 387 2903 387
Size: 2516 3647
NgoMIV G'CCGG_C
Cuts at: 2043 2043
Size: 6163
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6163
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 3088 3148 3379 3521 5066 1132
Size: 97 1859 60 231 142 1545 2229
Fragments arranged by size:
2229 1859 1545 231 142 97 60
PasI CC'CwG_GG
Cuts at: 1348 2195 2209 2431 1348
Size: 847 14 222 5080
Fragments arranged by size:
5080 847 222 14
PciI A'CATG_T
Cuts at: 3084 3375 5062 3084
Size: 291 1687 4185
Fragments arranged by size:
4185 1687 291
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2123 3003 3340 1108
Size: 1015 880 337 3931
Fragments arranged by size:
3931 1015 880 337
PfoI T'CCnGG_A
Cuts at: 2295 2295
Size: 6163
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 5745 5777 4232
Size: 32 1481 32 4618
Fragments arranged by size:
4618 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2109 2403 2427 2109
Size: 294 24 5845
Fragments arranged by size:
5845 294 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3733 3733
Size: 6163
PspOMI G'GGCC_C
Cuts at: 2205 2588 2205
Size: 383 5780
PstI C_TGCA'G
Cuts at: 830 2234 3515 830
Size: 1404 1281 3478
Fragments arranged by size:
3478 1404 1281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
PvuII CAG'CTG
Cuts at: 3232 3232
Size: 6163
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SanDI GG'GwC_CC
Cuts at: 2109 2403 2427 2109
Size: 294 24 5845
Fragments arranged by size:
5845 294 24
SbfI CC_TGCA'GG
Cuts at: 3515 3515
Size: 6163
ScaI AGT'ACT
Cuts at: 4411 6123 4411
Size: 1712 4451
SfcI C'TryA_G
Cuts at: 826 1045 2230 3511 4646 5327 5518 826
Size: 219 1185 1281 1135 681 191 1471
Fragments arranged by size:
1471 1281 1185 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6163
SmaI CCC'GGG
Cuts at: 1872 2594 3098 3490 1872
Size: 722 504 392 4545
Fragments arranged by size:
4545 722 504 392
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4267 5168 5430 820
Size: 197 926 2324 901 262 1553
Fragments arranged by size:
2324 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SspI AAT'ATT
Cuts at: 5 52 4087 5
Size: 47 4035 2081
Fragments arranged by size:
4035 2081 47
StuI AGG'CCT
Cuts at: 1517 2241 3164 1517
Size: 724 923 4516
Fragments arranged by size:
4516 923 724
StyI C'CwwG_G
Cuts at: 513 1065 2157 2236 3171 3602 513
Size: 552 1092 79 935 431 3074
Fragments arranged by size:
3074 1092 935 552 431 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2590 2792 2896 4190 4207 4360 4545
Size: 1255 1010 202 104 1294 17 153 185
Cuts at: 4545 4990 325
Size: 445 1498
Fragments arranged by size:
1498 1294 1255 1010 445 202 185 153
104 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 3378 4409
Size: 274 80 33 51 1443 276 1125 1031
Cuts at: 4409 6121 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1125 1031 276 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6123 6155 6123
Size: 32 6131
Tth111I GACn'n_nGTC
Cuts at: 1316 2765 3052 1316
Size: 1449 287 4427
Fragments arranged by size:
4427 1449 287
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2120 2350 2755 3100 2120
Size: 230 405 345 5183
Fragments arranged by size:
5183 405 345 230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6163
XmaI C'CCGG_G
Cuts at: 1870 2592 3096 3488 1870
Size: 722 504 392 4545
Fragments arranged by size:
4545 722 504 392
XmnI GAAnn'nnTTC
Cuts at: 4292 4292
Size: 6163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI