################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BciVI BclI BfaI BfrI BlnI BlpI BmyI Bpu1102I BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 735 GTATAC GT^[AC][GT]AC AfaI 852 GTAC GT^AC AlwI 841 GATCC GGATC(4/5) AseI 160 ATTAAT AT^TAAT AsnI 160 ATTAAT AT^TAAT Asp700I 246 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 423 GGTCC G^G[AT]CC BbsI 338 GTCTTC GAAGAC(2/6) BbvCI 741 CCTCAGC CCTCAGC(-5/-2) BbvI 356 GCTGC GCAGC(8/12) BcnI 382 CCCGG CC^[GC]GG BglI 796 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 40 CTGGAG CTGGAG(16/14) BpuAI 338 GTCTTC GAAGAC(2/6) BsaAI 51 CACGTA [CT]AC^GT[GA] BsaBI 453 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 913 GAGACC GGTCTC(1/5) BsmI 871 GCATTC GAATGC(1/-1) BsrBI 876 CCGCTC CCGCTC(-3/-3) Bst1107I 735 GTATAC GTA^TAC BstZ17I 735 GTATAC GTA^TAC Cac8I 271 GCCTGC GC[ACGT]^[ACGT]GC Cfr13I 423 GGTCC G^G[ACGT]CC DsaI 686 CCACGG C^C[GA][CT]GG EarI 787 CTCTTC CTCTTC(1/4) EcoRV 399 GATATC GAT^ATC Fnu4HI 356 GCTGC GC^[ACGT]GC HapII 383 CCGG C^CGG HincII 217 GTTGAC GT[CT]^[GA]AC HindII 217 GTTGAC GT[CT]^[GA]AC HpaII 383 CCGG C^CGG ItaI 356 GCTGC GC^[ACGT]GC Ksp632I 787 CTCTTC CTCTTC(1/4) MaeII 52 ACGT A^CGT MamI 453 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 594 CAATTG C^AATTG MslI 136 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 383 CCGG C^CGG MunI 594 CAATTG C^AATTG NciI 382 CCCGG CC^[GC]GG PacI 157 TTAATTAA TTAAT^TAA PshBI 160 ATTAAT AT^TAAT RsaI 852 GTAC GT^AC Sau96I 423 GGTCC G^G[ACGT]CC SfaNI 149 GCATC GCATC(5/9) SfcI 618 CTACAG C^T[GA][CT]AG SspI 558 AATATT AAT^ATT TseI 356 GCTGC G^C[AT]GC XmnI 246 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 333 CCGC CCGC(-3/-1) 876 CCGC BanI 231 GGCACC G^G[CT][GA]CC 438 GGTGCC BglII 36 AGATCT A^GATCT 362 AGATCT BsiYI 407 CCGTTGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 876 CCGCTCAAAGG BslI 407 CCGTTGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 876 CCGCTCAAAGG BsmAI 892 GAGAC GTCTC(1/5) 913 GAGAC BsrI 25 CCAGT ACTGG(1/-1) 202 CCAGT BstYI 36 AGATCT [GA]^GATC[CT] 362 AGATCT DraI 502 TTTAAA TTT^AAA 564 TTTAAA EcoT14I 235 CCTTGG C^C[AT][AT]GG 917 CCAAGG HphI 858 TCACC GGTGA(8/7) 904 TCACC MflI 36 AGATCT [GA]^GATC[CT] 362 AGATCT NdeI 546 CATATG CA^TATG 699 CATATG PshAI 331 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 750 GACAGAAGTC PvuII 343 CAGCTG CAG^CTG 354 CAGCTG StyI 235 CCTTGG C^C[AT][AT]GG 917 CCAAGG TaqI 609 TCGA T^CGA 826 TCGA Tsp45I 682 GTGAC ^GT[GC]AC 886 GTCAC TthHB8I 609 TCGA T^CGA 826 TCGA XhoII 36 AGATCT [GA]^GATC[CT] 362 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AcsI AluI ApoI BsaJI BssKI BstNI DdeI DpnII EcoRII HinfI MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIV Sau3AI ScrFI TfiI Tru9I Tsp509I TspRI