################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BbeI BbvCI BciVI BfrI BglII BlnI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII BstBI BstEII BstPI BstXI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SpeI Sse8387I SspBI SspI SwaI TfiI Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 969 CGCG CG^CG AcyI 358 GACGCC G[GA]^CG[CT]C AflIII 1199 ACATGT A^C[GA][CT]GT Alw44I 1231 GTGCAC G^TGCAC ApaLI 1231 GTGCAC G^TGCAC AspI 1363 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1313 TGCGCA TGC^GCA BalI 995 TGGCCA TGG^CCA BamHI 507 GGATCC G^GATCC BbrPI 490 CACGTG CAC^GTG BcgI 770 GCATCTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 791 TGATCA T^GATCA BfaI 1081 CTAG C^TAG BsaBI 993 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 358 GACGCC G[GA]^CG[CT]C BspLU11I 1199 ACATGT A^CATGT BsrDI 913 GCAATG GCAATG(2/0) BssSI 757 CTCGTG CACGAG(-5/-1) Bst1107I 1138 GTATAC GTA^TAC BstAPI 324 GCACGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 969 CGCG CG^CG BstYI 507 GGATCC [GA]^GATC[CT] BstZ17I 1138 GTATAC GTA^TAC DraIII 1340 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1226 CCGTGG C^C[GA][CT]GG FbaI 791 TGATCA T^GATCA FspI 1313 TGCGCA TGC^GCA HaeII 1299 GGCGCT [GA]GCGC^[CT] HgaI 358 GACGC GACGC(5/10) Hin1I 358 GACGCC G[GA]^CG[CT]C HincII 140 GTCAAC GT[CT]^[GA]AC HindII 140 GTCAAC GT[CT]^[GA]AC HindIII 1069 AAGCTT A^AGCTT MaeI 1081 CTAG C^TAG MamI 993 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 507 GGATCC [GA]^GATC[CT] MluNI 995 TGGCCA TGG^CCA MscI 995 TGGCCA TGG^CCA MvnI 969 CGCG CG^CG NdeI 1206 CATATG CA^TATG PflFI 1363 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 490 CACGTG CAC^GTG PmlI 490 CACGTG CAC^GTG PshAI 561 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 1280 ACCTGGT A^CC[AT]GGT SnaBI 744 TACGTA TAC^GTA StuI 1556 AGGCCT AGG^CCT Tth111I 1363 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 507 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1036 GTCTAC GT^[AC][GT]AC 1138 GTATAC AfaI 629 GTAC GT^AC 747 GTAC AocI 501 CCTCAGG CC^T[ACGT]AGG 616 CCTGAGG ApaI 470 GGGCCC GGGCC^C 1091 GGGCCC BbsI 568 GTCTTC GAAGAC(2/6) 1162 GTCTTC BglI 542 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 614 GCCCTGAGGGC BpmI 154 CTGGAG CTGGAG(16/14) 609 CTGGAG BpuAI 568 GTCTTC GAAGAC(2/6) 1162 GTCTTC BsaAI 490 CACGTG [CT]AC^GT[GA] 744 TACGTA BsmI 51 GAATGC GAATGC(1/-1) 907 GCATTC BspMI 857 ACCTGC ACCTGC(4/8) 102 GCAGGT BsrBI 1524 CCGCTC CCGCTC(-3/-3) 1327 GAGCGG BsrI 219 CCAGT ACTGG(1/-1) 269 CCAGT Bsu36I 501 CCTCAGG CC^T[ACGT]AGG 616 CCTGAGG EaeI 950 TGGCCG [CT]^GGCC[GA] 995 TGGCCA EarI 1273 CTCTTC CTCTTC(1/4) 1459 CTCTTC Eco81I 501 CCTCAGG CC^T[ACGT]AGG 616 CCTGAGG EcoT14I 1412 CCTTGG C^C[AT][AT]GG 1541 CCAAGG HinfI 480 GAGTC G^A[ACGT]TC 832 GACTC Ksp632I 1273 CTCTTC CTCTTC(1/4) 1459 CTCTTC MseI 838 TTAA T^TAA 1471 TTAA PleI 832 GACTC GAGTC(4/5) 480 GAGTC PstI 722 CTGCAG CTGCA^G 765 CTGCAG RsaI 629 GTAC GT^AC 747 GTAC SacI 120 GAGCTC GAGCT^C 1131 GAGCTC SfcI 722 CTGCAG C^T[GA][CT]AG 765 CTGCAG StyI 1412 CCTTGG C^C[AT][AT]GG 1541 CCAAGG TaqI 1001 TCGA T^CGA 1166 TCGA Tru9I 838 TTAA T^TAA 1471 TTAA TthHB8I 1001 TCGA T^CGA 1166 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BlpI BmyI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PvuII Sau3AI Sau96I ScrFI SfaNI SmaI SphI TseI Tsp45I TspRI Van91I XcmI XmaCI XmaI