(Circular) (Six-base) MAPSORT of: pFN21AE9455.seq Check: 1611 from: 1 to: 6289 pFN21AE9455 6289 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2098 2098 Size: 6289 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4099 278 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3042 3144 3632 1937 Size: 1105 102 488 4594 Fragments arranged by size: 4594 1105 488 102 Acc65I G'GTAC_C Cuts at: 3610 3610 Size: 6289 AclI AA'CG_TT Cuts at: 1563 4416 4789 1563 Size: 2853 373 3063 Fragments arranged by size: 3063 2853 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2306 3550 4352 5736 6004 1632 Size: 674 1244 802 1384 268 1917 Fragments arranged by size: 1917 1384 1244 802 674 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6092 AflIII A'CryG_T Cuts at: 1203 3204 5188 6165 1203 Size: 2001 1984 977 1327 Fragments arranged by size: 2001 1984 1327 977 AgeI A'CCGG_T Cuts at: 5120 5120 Size: 6289 AhdI GACnn_n'nnGTC Cuts at: 5018 5018 Size: 6289 AleI CACnn'nnGTG Cuts at: 1166 2016 1166 Size: 850 5439 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6289 AlwNI CAG_nnn'CTG Cuts at: 2230 2532 3360 4032 5604 2230 Size: 302 828 672 1572 2915 Fragments arranged by size: 2915 1572 828 672 302 ApaI G_GGCC'C Cuts at: 2479 3100 2479 Size: 621 5668 ApaLI G'TGCA_C Cuts at: 3236 4346 5502 3236 Size: 1110 1156 4023 Fragments arranged by size: 4023 1156 1110 ApoI r'AATT_y Cuts at: 1493 3598 3824 3976 6085 1493 Size: 2105 226 152 2109 1697 Fragments arranged by size: 2109 2105 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4843 160 Size: 872 3811 1606 Fragments arranged by size: 3811 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 AvaI C'yCGr_G Cuts at: 1870 1943 2319 2358 2933 3032 3614 1870 Size: 73 376 39 575 99 582 4545 Fragments arranged by size: 4545 582 575 376 99 73 39 BamHI G'GATC_C Cuts at: 2512 3619 3986 2512 Size: 1107 367 4815 Fragments arranged by size: 4815 1107 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2293 2740 3525 3610 Size: 325 217 627 231 275 447 785 85 Cuts at: 3610 5065 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 785 627 447 325 275 231 217 85 BanII G_rGCy'C Cuts at: 1391 2129 2479 2621 2909 3100 3140 3230 3608 Size: 738 350 142 288 191 40 90 378 Cuts at: 3608 1391 Size: 4072 Fragments arranged by size: 4072 738 378 350 288 191 142 90 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2566 3160 928 Size: 971 667 594 4057 Fragments arranged by size: 4057 971 667 594 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2764 2798 4480 4514 1903 Size: 34 827 34 1682 34 3678 Fragments arranged by size: 3678 1682 827 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4181 5391 4181 Size: 1210 5079 BclI T'GATC_A Cuts at: 1548 2796 1548 Size: 1248 5041 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2553 2625 4900 Size: 107 122 71 631 605 881 72 2275 Cuts at: 4900 136 Size: 1525 Fragments arranged by size: 2275 1525 881 631 605 122 107 72 71 BglII A'GATC_T Cuts at: 6284 6284 Size: 6289 BlpI GC'TnA_GC Cuts at: 2243 2903 2981 3706 2243 Size: 660 78 725 4826 Fragments arranged by size: 4826 725 660 78 Bme1580I G_kGCm'C Cuts at: 1790 2023 2298 2479 2496 2934 3100 3240 4350 Size: 233 275 181 17 438 166 140 1110 Cuts at: 4350 5506 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1110 438 275 233 181 166 140 17 BmgBI CAC'GTC Cuts at: 1318 3273 1318 Size: 1955 4334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4020 4978 442 Size: 416 53 83 399 24 2603 958 1753 Fragments arranged by size: 2603 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6289 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2662 2662 Size: 6289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2635 4949 1239 Size: 941 455 2314 2579 Fragments arranged by size: 2579 2314 941 455 Bpu10I CC'TnA_GC Cuts at: 2087 2087 Size: 6289 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4414 5279 5577 4414 Size: 865 298 5126 Fragments arranged by size: 5126 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4952 882 Size: 617 3453 2219 Fragments arranged by size: 3453 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2497 2751 4080 493 Size: 711 1293 254 1329 2702 Fragments arranged by size: 2702 1329 1293 711 254 BsaBI GATnn'nnATC Cuts at: 3002 6283 3002 Size: 3281 3008 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2364 4096 4478 5113 Size: 53 83 186 936 831 1732 382 635 Cuts at: 5113 275 Size: 1451 Fragments arranged by size: 1732 1451 936 831 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4722 5120 5394 5541 5952 4722 Size: 398 274 147 411 5059 Fragments arranged by size: 5059 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6064 Fragments arranged by size: 6064 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3017 3020 3562 1186 Size: 1831 3 542 3913 Fragments arranged by size: 3913 1831 542 3 BseYI C'CCAG_C Cuts at: 2478 2713 3090 3100 3177 3216 3382 3400 3453 Size: 235 377 10 77 39 166 18 53 Cuts at: 3453 3519 5492 5988 2478 Size: 66 1973 496 2779 Fragments arranged by size: 2779 1973 496 377 235 166 77 66 53 39 18 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2795 2951 3139 1163 Size: 573 1059 156 188 4313 Fragments arranged by size: 4313 1059 573 188 156 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3739 4500 5528 1540 Size: 275 186 1738 761 1028 2301 Fragments arranged by size: 2301 1738 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2129 2664 3140 3240 4350 4435 5506 2129 Size: 535 476 100 1110 85 1071 2912 Fragments arranged by size: 2912 1110 1071 535 476 100 85 BsmI GAATG_Cn' Cuts at: 2062 2912 3800 3893 2062 Size: 850 888 93 4458 Fragments arranged by size: 4458 888 850 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2129 2298 2479 2496 2621 2664 Size: 399 233 106 169 181 17 125 43 Cuts at: 2664 2909 2934 3100 3140 3230 3240 3608 4350 Size: 245 25 166 40 90 10 368 742 Cuts at: 4350 4435 5506 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 368 245 233 181 169 166 125 106 90 85 43 40 25 17 10 BspEI T'CCGG_A Cuts at: 5952 5952 Size: 6289 BspHI T'CATG_A Cuts at: 4178 5908 4178 Size: 1730 4559 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2098 2871 3644 844 Size: 1254 773 773 3489 Fragments arranged by size: 3489 1254 773 773 BsrBI CCG'CTC Cuts at: 1394 1823 3334 3531 4176 1394 Size: 429 1511 197 645 3507 Fragments arranged by size: 3507 1511 645 429 197 BsrDI GCAATG_nn' Cuts at: 65 1248 2925 4784 4958 65 Size: 1183 1677 1859 174 1396 Fragments arranged by size: 1859 1677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4933 5120 1700 Size: 110 3123 187 2869 Fragments arranged by size: 3123 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6289 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6289 BssSI C'ACGA_G Cuts at: 2762 4349 5361 2762 Size: 1587 1012 3690 Fragments arranged by size: 3690 1587 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2335 794 Size: 1541 4748 BstBI TT'CG_AA Cuts at: 5093 5093 Size: 6289 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6289 BstYI r'GATC_y Cuts at: 1972 2512 3619 3986 4371 4388 5840 5926 5938 Size: 540 1107 367 385 17 1452 86 12 Cuts at: 5938 6284 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1107 540 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3145 3145 Size: 6289 Bsu36I CC'TnA_GG Cuts at: 2507 2622 2507 Size: 115 6174 BtgI C'CryG_G Cuts at: 513 1065 2004 3231 513 Size: 552 939 1227 3571 Fragments arranged by size: 3571 1227 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2875 487 Size: 683 56 1649 3901 Fragments arranged by size: 3901 1649 683 56 BtsI GCAGTG_nn' Cuts at: 3805 4599 4619 3805 Size: 794 20 5475 Fragments arranged by size: 5475 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6289 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6254 DraI TTT'AAA Cuts at: 3593 3593 Size: 6289 DraIII CAC_nnn'GTG Cuts at: 3350 3350 Size: 6289 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5296 809 Size: 507 3980 1802 Fragments arranged by size: 3980 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2955 3000 3736 Size: 54 1425 50 275 18 1125 45 736 Cuts at: 3736 4625 6254 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1125 889 736 275 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3736 1537 Size: 275 1924 4090 Fragments arranged by size: 4090 1924 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3284 3470 4219 1353 Size: 1931 186 749 3423 Fragments arranged by size: 3423 1931 749 186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3353 4876 5250 5396 387 Size: 1064 138 1764 1523 374 146 1280 Fragments arranged by size: 1764 1523 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2127 3138 2127 Size: 1011 5278 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2306 2635 3550 4352 4949 5736 Size: 393 548 126 329 915 802 597 787 Cuts at: 5736 6004 1239 Size: 268 1524 Fragments arranged by size: 1524 915 802 787 597 548 393 329 268 126 EcoO109I rG'GnC_Cy Cuts at: 2475 3096 3097 3357 2475 Size: 621 1 260 5407 Fragments arranged by size: 5407 621 260 1 EcoRI G'AATT_C Cuts at: 3598 3976 3598 Size: 378 5911 FspI TGC'GCA Cuts at: 3320 4795 3320 Size: 1475 4814 HaeII r_GCGC'y Cuts at: 1678 3308 5436 1678 Size: 1630 2128 2531 Fragments arranged by size: 2531 2128 1630 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4930 4962 5004 5036 1373 Size: 32 135 32 3358 32 42 32 2626 Fragments arranged by size: 3358 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2147 3633 3879 669 Size: 988 281 209 1486 246 3079 Fragments arranged by size: 3079 1486 988 281 246 209 HindIII A'AGCT_T Cuts at: 748 3074 748 Size: 2326 3963 HpaI GTT'AAC Cuts at: 3879 3879 Size: 6289 KpnI G_GTAC'C Cuts at: 3614 3614 Size: 6289 MfeI C'AATT_G Cuts at: 3888 3888 Size: 6289 MluI A'CGCG_T Cuts at: 6165 6165 Size: 6289 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2439 5403 5587 1305 Size: 1134 2964 184 2007 Fragments arranged by size: 2964 2007 1134 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3002 10 Size: 54 1425 343 1170 3297 Fragments arranged by size: 3297 1425 1170 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2016 2855 2996 4247 4606 4765 518 Size: 648 850 839 141 1251 359 159 2042 Fragments arranged by size: 2042 1251 850 839 648 359 159 141 MspA1I CmG'CkG Cuts at: 1606 2158 2482 2670 2960 2985 3177 3457 3523 Size: 552 324 188 290 25 192 280 66 Cuts at: 3523 3705 4382 5530 5775 1606 Size: 182 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 552 324 290 280 245 192 188 182 66 25 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4798 Fragments arranged by size: 4798 939 552 NdeI CA'TA_TG Cuts at: 387 3212 387 Size: 2825 3464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6289 NotI GC'GGCC_GC Cuts at: 3736 3736 Size: 6289 NspI r_CATG'y Cuts at: 1132 1229 2318 2870 2946 3208 3647 5192 1132 Size: 97 1089 552 76 262 439 1545 2229 Fragments arranged by size: 2229 1545 1089 552 439 262 97 76 PasI CC'CwG_GG Cuts at: 1348 2382 2604 1348 Size: 1034 222 5033 Fragments arranged by size: 5033 1034 222 PciI A'CATG_T Cuts at: 3204 5188 3204 Size: 1984 4305 PflMI CCAn_nnn'nTGG Cuts at: 1108 2158 2230 2548 3216 1108 Size: 1050 72 318 668 4181 Fragments arranged by size: 4181 1050 668 318 72 PmeI GTTT'AAAC Cuts at: 3593 3593 Size: 6289 PmlI CAC'GTG Cuts at: 2497 2497 Size: 6289 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4358 4390 5871 5903 4358 Size: 32 1481 32 4744 Fragments arranged by size: 4744 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2570 1613 Size: 957 5332 PsiI TTA'TAA Cuts at: 3859 3859 Size: 6289 PspOMI G'GGCC_C Cuts at: 2475 3096 2475 Size: 621 5668 PstI C_TGCA'G Cuts at: 830 2731 2774 3641 830 Size: 1901 43 867 3478 Fragments arranged by size: 3478 1901 867 43 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6289 PvuII CAG'CTG Cuts at: 2158 2482 2985 3177 3457 2158 Size: 324 503 192 280 4990 Fragments arranged by size: 4990 503 324 280 192 SacI G_AGCT'C Cuts at: 2129 3140 2129 Size: 1011 5278 SalI G'TCGA_C Cuts at: 1936 3631 1936 Size: 1695 4594 SbfI CC_TGCA'GG Cuts at: 3641 3641 Size: 6289 ScaI AGT'ACT Cuts at: 4537 6249 4537 Size: 1712 4577 SexAI A'CCwGG_T Cuts at: 3285 3285 Size: 6289 SfcI C'TryA_G Cuts at: 826 1045 2727 2770 3637 4772 5453 5644 826 Size: 219 1682 43 867 1135 681 191 1471 Fragments arranged by size: 1682 1471 1135 867 681 219 191 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6289 SmaI CCC'GGG Cuts at: 1872 2321 2360 2935 3616 1872 Size: 449 39 575 681 4545 Fragments arranged by size: 4545 681 575 449 39 SmlI C'TyrA_G Cuts at: 820 1017 1943 4393 5294 5556 820 Size: 197 926 2450 901 262 1553 Fragments arranged by size: 2450 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2751 4080 493 Size: 2258 1329 2702 Fragments arranged by size: 2702 2258 1329 SpeI A'CTAG_T Cuts at: 152 152 Size: 6289 SphI G_CATG'C Cuts at: 1132 2318 2870 2946 3647 1132 Size: 1186 552 76 701 3774 Fragments arranged by size: 3774 1186 701 552 76 SspI AAT'ATT Cuts at: 5 52 4213 5 Size: 47 4161 2081 Fragments arranged by size: 4161 2081 47 StuI AGG'CCT Cuts at: 1517 3563 1517 Size: 2046 4243 StyI C'CwwG_G Cuts at: 513 1065 2004 3417 3546 3728 513 Size: 552 939 1413 129 182 3074 Fragments arranged by size: 3074 1413 939 552 182 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2230 2435 3208 4316 4333 4486 4671 Size: 1255 650 205 773 1108 17 153 185 Cuts at: 4671 5116 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1108 773 650 445 205 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4535 6247 96 Size: 274 80 33 51 1443 2558 1712 138 Fragments arranged by size: 2558 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2205 2237 2818 2850 6249 6281 2205 Size: 32 581 32 3399 32 2213 Fragments arranged by size: 3399 2213 581 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3371 1316 Size: 2055 4234 XbaI T'CTAG_A Cuts at: 3625 3625 Size: 6289 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2227 2486 3415 2227 Size: 259 929 5101 Fragments arranged by size: 5101 929 259 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6289 XmaI C'CCGG_G Cuts at: 1870 2319 2358 2933 3614 1870 Size: 449 39 575 681 4545 Fragments arranged by size: 4545 681 575 449 39 XmnI GAAnn'nnTTC Cuts at: 4418 4418 Size: 6289 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4097 276 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI