(Linear) (Six-base) MAPSORT of: KIEE9455.seq Check: 5406 from: 1 to: 1584 KIEE9455.orf With 182 enzymes: SgfI * December 27, 2011 16:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 93 1584 Size: 93 1491 AccI GT'mk_AC Cuts at: 0 1037 1139 1584 Size: 1037 102 445 Fragments arranged by size: 1037 445 102 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 1545 1584 Size: 301 1244 39 Fragments arranged by size: 1244 301 39 AflIII A'CryG_T Cuts at: 0 1199 1584 Size: 1199 385 AleI CACnn'nnGTG Cuts at: 0 11 1584 Size: 11 1573 AlwNI CAG_nnn'CTG Cuts at: 0 225 527 1355 1584 Size: 225 302 828 229 Fragments arranged by size: 828 302 229 225 ApaI G_GGCC'C Cuts at: 0 474 1095 1584 Size: 474 621 489 Fragments arranged by size: 621 489 474 ApaLI G'TGCA_C Cuts at: 0 1231 1584 Size: 1231 353 AvaI C'yCGr_G Cuts at: 0 314 353 928 1027 1584 Size: 314 39 575 99 557 Fragments arranged by size: 575 557 314 99 39 BamHI G'GATC_C Cuts at: 0 507 1584 Size: 507 1077 BanI G'GyrC_C Cuts at: 0 13 288 735 1520 1584 Size: 13 275 447 785 64 Fragments arranged by size: 785 447 275 64 13 BanII G_rGCy'C Cuts at: 0 124 474 616 904 1095 1135 1225 1584 Size: 124 350 142 288 191 40 90 359 Fragments arranged by size: 359 350 288 191 142 124 90 40 BbsI GAAGACnn'nnnn_ Cuts at: 0 561 1155 1584 Size: 561 594 429 Fragments arranged by size: 594 561 429 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 759 793 1584 Size: 759 34 791 Fragments arranged by size: 791 759 34 BclI T'GATC_A Cuts at: 0 791 1584 Size: 791 793 BglI GCCn_nnn'nGGC Cuts at: 0 548 620 1584 Size: 548 72 964 Fragments arranged by size: 964 548 72 BlpI GC'TnA_GC Cuts at: 0 238 898 976 1584 Size: 238 660 78 608 Fragments arranged by size: 660 608 238 78 Bme1580I G_kGCm'C Cuts at: 0 18 293 474 491 929 1095 1235 1584 Size: 18 275 181 17 438 166 140 349 Fragments arranged by size: 438 349 275 181 166 140 18 17 BmgBI CAC'GTC Cuts at: 0 1268 1584 Size: 1268 316 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 657 1584 Size: 657 927 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 630 1584 Size: 175 455 954 Fragments arranged by size: 954 455 175 Bpu10I CC'TnA_GC Cuts at: 0 82 1584 Size: 82 1502 BsaAI yAC'GTr Cuts at: 0 492 746 1584 Size: 492 254 838 Fragments arranged by size: 838 492 254 BsaBI GATnn'nnATC Cuts at: 0 997 1584 Size: 997 587 BsaHI Gr'CG_yC Cuts at: 0 359 1584 Size: 359 1225 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1012 1015 1557 1584 Size: 1012 3 542 27 Fragments arranged by size: 1012 542 27 3 BseYI C'CCAG_C Cuts at: 0 473 708 1085 1095 1172 1211 1377 1395 Size: 473 235 377 10 77 39 166 18 Cuts at: 1395 1448 1514 1584 Size: 53 66 70 Fragments arranged by size: 473 377 235 166 77 70 66 53 39 18 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 790 946 1134 1584 Size: 790 156 188 450 Fragments arranged by size: 790 450 188 156 BsiHKAI G_wGCw'C Cuts at: 0 124 659 1135 1235 1584 Size: 124 535 476 100 349 Fragments arranged by size: 535 476 349 124 100 BsmI GAATG_Cn' Cuts at: 0 57 907 1584 Size: 57 850 677 Fragments arranged by size: 850 677 57 Bsp1286I G_dGCh'C Cuts at: 0 18 124 293 474 491 616 659 904 Size: 18 106 169 181 17 125 43 245 Cuts at: 904 929 1095 1135 1225 1235 1584 Size: 25 166 40 90 10 349 Fragments arranged by size: 349 245 181 169 166 125 106 90 43 40 25 18 17 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 93 866 1584 Size: 93 773 718 Fragments arranged by size: 773 718 93 BsrBI CCG'CTC Cuts at: 0 1329 1526 1584 Size: 1329 197 58 Fragments arranged by size: 1329 197 58 BsrDI GCAATG_nn' Cuts at: 0 920 1584 Size: 920 664 BssSI C'ACGA_G Cuts at: 0 757 1584 Size: 757 827 BstAPI GCAn_nnn'nTGC Cuts at: 0 330 1584 Size: 330 1254 BstYI r'GATC_y Cuts at: 0 507 1584 Size: 507 1077 BstZ17I GTA'TAC Cuts at: 0 1140 1584 Size: 1140 444 Bsu36I CC'TnA_GG Cuts at: 0 502 617 1584 Size: 502 115 967 Fragments arranged by size: 967 502 115 BtgI C'CryG_G Cuts at: 0 1226 1584 Size: 1226 358 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 870 1584 Size: 870 714 DraIII CAC_nnn'GTG Cuts at: 0 1345 1584 Size: 1345 239 EaeI y'GGCC_r Cuts at: 0 950 995 1584 Size: 950 45 589 Fragments arranged by size: 950 589 45 EarI CTCTTCn'nnn_ Cuts at: 0 1279 1465 1584 Size: 1279 186 119 Fragments arranged by size: 1279 186 119 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1348 1584 Size: 1348 236 EcoICRI GAG'CTC Cuts at: 0 122 1133 1584 Size: 122 1011 451 Fragments arranged by size: 1011 451 122 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 301 630 1545 1584 Size: 175 126 329 915 39 Fragments arranged by size: 915 329 175 126 39 EcoO109I rG'GnC_Cy Cuts at: 0 470 1091 1092 1352 1584 Size: 470 621 1 260 232 Fragments arranged by size: 621 470 260 232 1 FspI TGC'GCA Cuts at: 0 1315 1584 Size: 1315 269 HaeII r_GCGC'y Cuts at: 0 1303 1584 Size: 1303 281 HincII GTy'rAC Cuts at: 0 142 1584 Size: 142 1442 HindIII A'AGCT_T Cuts at: 0 1069 1584 Size: 1069 515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 434 1584 Size: 434 1150 MscI TGG'CCA Cuts at: 0 997 1584 Size: 997 587 MslI CAynn'nnrTG Cuts at: 0 11 850 991 1584 Size: 11 839 141 593 Fragments arranged by size: 839 593 141 11 MspA1I CmG'CkG Cuts at: 0 153 477 665 955 980 1172 1452 1518 Size: 153 324 188 290 25 192 280 66 Cuts at: 1518 1584 Size: 66 Fragments arranged by size: 324 290 280 192 188 153 66 66 25 NdeI CA'TA_TG Cuts at: 0 1207 1584 Size: 1207 377 NspI r_CATG'y Cuts at: 0 313 865 941 1203 1584 Size: 313 552 76 262 381 Fragments arranged by size: 552 381 313 262 76 PasI CC'CwG_GG Cuts at: 0 377 599 1584 Size: 377 222 985 Fragments arranged by size: 985 377 222 PciI A'CATG_T Cuts at: 0 1199 1584 Size: 1199 385 PflMI CCAn_nnn'nTGG Cuts at: 0 153 225 543 1211 1584 Size: 153 72 318 668 373 Fragments arranged by size: 668 373 318 153 72 PmlI CAC'GTG Cuts at: 0 492 1584 Size: 492 1092 PshAI GACnn'nnGTC Cuts at: 0 565 1584 Size: 565 1019 PspOMI G'GGCC_C Cuts at: 0 470 1091 1584 Size: 470 621 493 Fragments arranged by size: 621 493 470 PstI C_TGCA'G Cuts at: 0 726 769 1584 Size: 726 43 815 Fragments arranged by size: 815 726 43 PvuII CAG'CTG Cuts at: 0 153 477 980 1172 1452 1584 Size: 153 324 503 192 280 132 Fragments arranged by size: 503 324 280 192 153 132 SacI G_AGCT'C Cuts at: 0 124 1135 1584 Size: 124 1011 449 Fragments arranged by size: 1011 449 124 SexAI A'CCwGG_T Cuts at: 0 1280 1584 Size: 1280 304 SfcI C'TryA_G Cuts at: 0 722 765 1584 Size: 722 43 819 Fragments arranged by size: 819 722 43 SmaI CCC'GGG Cuts at: 0 316 355 930 1584 Size: 316 39 575 654 Fragments arranged by size: 654 575 316 39 SnaBI TAC'GTA Cuts at: 0 746 1584 Size: 746 838 SphI G_CATG'C Cuts at: 0 313 865 941 1584 Size: 313 552 76 643 Fragments arranged by size: 643 552 313 76 StuI AGG'CCT Cuts at: 0 1558 1584 Size: 1558 26 StyI C'CwwG_G Cuts at: 0 1412 1541 1584 Size: 1412 129 43 Fragments arranged by size: 1412 129 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 225 430 1203 1584 Size: 225 205 773 381 Fragments arranged by size: 773 381 225 205 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 200 232 813 845 1584 Size: 200 32 581 32 739 Fragments arranged by size: 739 581 200 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1366 1584 Size: 1366 218 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 222 481 1410 1584 Size: 222 259 929 174 Fragments arranged by size: 929 259 222 174 XmaI C'CCGG_G Cuts at: 0 314 353 928 1584 Size: 314 39 575 656 Fragments arranged by size: 656 575 314 39 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI BcgI BclI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BssSI BstAPI BstYI BstZ17I Bsu36I BtgI BtgZI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII HincII HindIII MmeI MscI MslI MspA1I NdeI NspI PasI PciI PflMI PmlI PshAI PspOMI PstI PvuII SacI SexAI SfcI SmaI SnaBI SphI StuI StyI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BfrBI BglII BmrI BmtI BpuEI BsaI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrFI BsrGI BssHII BstBI BstEII BstXI BtsI ClaI CspCI DraI DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SpeI SrfI SspI SwaI TatI XbaI XhoI XmnI ZraI