################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 980 GCGG CCGC(-3/-1) AflII 990 CTTAAG C^TTAAG AflIII 325 ACATGT A^C[GA][CT]GT AlwI 406 GATCC GGATC(4/5) AlwNI 306 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 6 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 668 TGGCCA TGG^CCA BbvCI 22 GCTGAGG CCTCAGC(-5/-2) BfaI 683 CTAG C^TAG BfrI 990 CTTAAG C^TTAAG BsaBI 162 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 187 CTGCAC GTGCAG(16/14) BsiYI 18 CCAAGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 18 CCAAGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 302 GGGAC GGGAC(10/14) BspHI 158 TCATGA T^CATGA BspLU11I 325 ACATGT A^CATGT BsrDI 393 CATTGC GCAATG(2/0) BstF5I 338 CATCC GGATG(2/0) DrdI 996 GACTGATTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 635 CCATGG C^C[GA][CT]GG EaeI 668 TGGCCA [CT]^GGCC[GA] EcoT22I 453 ATGCAT ATGCA^T FokI 338 CATCC GGATG(9/13) HgaI 214 GCGTC GACGC(5/10) HincII 1005 GTCAAC GT[CT]^[GA]AC HindII 1005 GTCAAC GT[CT]^[GA]AC HphI 3 GGTGA GGTGA(8/7) MaeI 683 CTAG C^TAG MaeII 50 ACGT A^CGT MamI 162 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 668 TGGCCA TGG^CCA MscI 668 TGGCCA TGG^CCA MslI 249 CACTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 635 CCATGG C^CATGG NsiI 453 ATGCAT ATGCA^T NspI 325 ACATGT [GA]CATG^[CT] PleI 245 GAGTC GAGTC(4/5) RcaI 158 TCATGA T^CATGA SmlI 990 CTTAAG C^T[CT][GA]AG XcmI 626 CCAATGTCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 6 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 316 AAATTC [GA]^AATT[CT] 852 GAATTT AfaI 294 GTAC GT^AC 698 GTAC ApoI 316 AAATTC [GA]^AATT[CT] 852 GAATTT AvaII 88 GGTCC G^G[AT]CC 594 GGTCC BseRI 720 CTCCTC GAGGAG(10/8) 863 CTCCTC BsmAI 1036 GAGAC GTCTC(1/5) 805 GTCTC BsrI 153 ACTGG ACTGG(1/-1) 590 ACTGG BstXI 770 CCAAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 945 CCAATGGTTTGG Cfr13I 88 GGTCC G^G[ACGT]CC 594 GGTCC DraII 87 AGGTCCT [GA]G^G[ACGT]CC[CT] 593 GGGTCCT EcoO109I 87 AGGTCCT [GA]G^G[ACGT]CC[CT] 593 GGGTCCT HaeIII 222 GGCC GG^CC 669 GGCC MseI 68 TTAA T^TAA 991 TTAA PpuMI 87 AGGTCCT [GA]G^G[AT]CC[CT] 593 GGGTCCT RsaI 294 GTAC GT^AC 698 GTAC Sau96I 88 GGTCC G^G[ACGT]CC 594 GGTCC TaqI 93 TCGA T^CGA 496 TCGA Tru9I 68 TTAA T^TAA 991 TTAA Tsp45I 247 GTCAC ^GT[GC]AC 481 GTCAC TthHB8I 93 TCGA T^CGA 496 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AluI BbvI BsaJI BssKI BstNI Cac8I DdeI DpnII EcoRII EcoT14I Fnu4HI HinfI ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI StyI TfiI TseI Tsp509I TspRI