(Circular) (Six-base) MAPSORT of: pFN21AE9421.seq Check: 8812 from: 1 to: 5749 pFN21AE9421 5749 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3092 1937 Size: 1155 4594 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2251 3051 3812 5196 5464 1632 Size: 619 800 761 1384 268 1917 Fragments arranged by size: 1917 1384 800 761 619 268 AflII C'TTAA_G Cuts at: 820 1017 2995 820 Size: 197 1978 3574 Fragments arranged by size: 3574 1978 197 AflIII A'CryG_T Cuts at: 1203 2330 4648 5625 1203 Size: 1127 2318 977 1327 Fragments arranged by size: 2318 1327 1127 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2003 2035 2003 Size: 32 5717 AlwNI CAG_nnn'CTG Cuts at: 2316 3492 5064 2316 Size: 1176 1572 3001 Fragments arranged by size: 3001 1572 1176 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 2321 2857 3058 3284 3436 5545 1493 Size: 828 536 201 226 152 2109 1697 Fragments arranged by size: 2109 1697 828 536 226 201 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 3074 1870 Size: 73 1131 4545 Fragments arranged by size: 4545 1131 73 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3070 4525 618 Size: 325 217 627 1283 1455 1842 Fragments arranged by size: 1842 1455 1283 627 325 217 BanII G_rGCy'C Cuts at: 1391 3068 1391 Size: 1677 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4778 BbvCI CC'TCA_GC Cuts at: 2028 2028 Size: 5749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BfrBI ATG'CAT Cuts at: 2460 2460 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4360 136 Size: 107 122 71 631 605 2688 1525 Fragments arranged by size: 2688 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 3810 4966 1790 Size: 2020 1156 2573 Fragments arranged by size: 2573 2020 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2605 3480 4438 Size: 416 53 83 399 24 1188 875 958 Cuts at: 4438 442 Size: 1753 Fragments arranged by size: 1753 1188 958 875 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4409 1239 Size: 3170 2579 Bpu10I CC'TnA_GC Cuts at: 2028 2028 Size: 5749 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3874 4739 5037 3874 Size: 865 298 4586 Fragments arranged by size: 4586 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4412 882 Size: 617 2913 2219 Fragments arranged by size: 2913 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3540 493 Size: 711 2336 2702 Fragments arranged by size: 2702 2336 711 BsaBI GATnn'nnATC Cuts at: 2171 5743 2171 Size: 3572 2177 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3556 3938 4573 275 Size: 53 83 186 936 2023 382 635 1451 Fragments arranged by size: 2023 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 2241 2271 1178 Size: 30 165 30 600 30 208 30 4656 Fragments arranged by size: 4656 600 208 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2716 2859 1186 Size: 1530 143 4076 Fragments arranged by size: 4076 1530 143 BseYI C'CCAG_C Cuts at: 4952 5448 4952 Size: 496 5253 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 1163 Size: 573 441 4735 Fragments arranged by size: 4735 573 441 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3199 3960 4988 1540 Size: 275 186 1198 761 1028 2301 Fragments arranged by size: 2301 1198 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3810 3895 4966 3810 Size: 85 1071 4593 Fragments arranged by size: 4593 1071 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3068 3810 3895 4966 1391 Size: 399 1278 742 85 1071 2174 Fragments arranged by size: 2174 1278 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 2163 3638 5368 2163 Size: 1475 1730 2544 Fragments arranged by size: 2544 1730 1475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3104 844 Size: 2260 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3636 1394 Size: 429 1813 3507 Fragments arranged by size: 3507 1813 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2397 4244 4418 65 Size: 1183 1149 1847 174 1396 Fragments arranged by size: 1847 1396 1183 1149 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4393 4580 1700 Size: 110 2583 187 2869 Fragments arranged by size: 2869 2583 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2782 2957 2782 Size: 175 5574 BstYI r'GATC_y Cuts at: 1972 3079 3446 3831 3848 5300 5386 5398 5744 Size: 1107 367 385 17 1452 86 12 346 Cuts at: 5744 1972 Size: 1977 Fragments arranged by size: 1977 1452 1107 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2640 513 Size: 552 939 636 3622 Fragments arranged by size: 3622 939 636 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 2844 3265 4059 4079 2844 Size: 421 794 20 4514 Fragments arranged by size: 4514 794 421 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3007 4756 809 Size: 507 1691 1749 1802 Fragments arranged by size: 1802 1749 1691 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2673 3196 4085 Size: 54 1425 50 275 18 843 523 889 Cuts at: 4085 5714 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 843 523 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3679 1353 Size: 2326 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2251 3051 3812 4409 5196 5464 1239 Size: 393 619 800 761 597 787 268 1524 Fragments arranged by size: 1524 800 787 761 619 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2093 2599 2093 Size: 506 5243 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 4896 1678 Size: 3218 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2091 2123 4390 4422 4464 Size: 32 135 32 519 32 2267 32 42 Cuts at: 4464 4496 1373 Size: 32 2626 Fragments arranged by size: 2626 2267 519 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3093 3339 669 Size: 988 281 1074 81 246 3079 Fragments arranged by size: 3079 1074 988 281 246 81 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2450 4863 5047 1305 Size: 1145 2413 184 2007 Fragments arranged by size: 2413 2007 1145 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 10 Size: 54 1425 343 843 3084 Fragments arranged by size: 3084 1425 843 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2258 3707 4066 4225 518 Size: 648 1092 1449 359 159 2042 Fragments arranged by size: 2042 1449 1092 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3165 3842 4990 5235 1606 Size: 1559 677 1148 245 2120 Fragments arranged by size: 2120 1559 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2640 513 Size: 552 939 636 3622 Fragments arranged by size: 3622 939 636 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NsiI A_TGCA'T Cuts at: 2462 2462 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 2334 3107 4652 1132 Size: 97 1105 773 1545 2229 Fragments arranged by size: 2229 1545 1105 773 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5749 PciI A'CATG_T Cuts at: 2330 4648 2330 Size: 2318 3431 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5749 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2003 2035 3818 3850 5331 5363 2003 Size: 32 1783 32 1481 32 2389 Fragments arranged by size: 2389 1783 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2093 2599 2093 Size: 506 5243 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 2864 2910 3319 2864 Size: 46 409 5294 Fragments arranged by size: 5294 409 46 PstI C_TGCA'G Cuts at: 830 3101 830 Size: 2271 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SfcI C'TryA_G Cuts at: 826 1045 3097 4232 4913 5104 826 Size: 219 2052 1135 681 191 1471 Fragments arranged by size: 2052 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 3076 1872 Size: 1204 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2995 3853 4754 5016 820 Size: 197 926 1052 858 901 262 1553 Fragments arranged by size: 1553 1052 926 901 858 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5749 StyI C'CwwG_G Cuts at: 513 1065 2004 2119 2640 2781 3188 513 Size: 552 939 115 521 141 407 3074 Fragments arranged by size: 3074 939 552 521 407 141 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3776 3793 3946 4131 4576 325 Size: 1255 2196 17 153 185 445 1498 Fragments arranged by size: 2196 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2702 3995 5707 Size: 274 80 33 51 1443 725 1293 1712 Cuts at: 5707 96 Size: 138 Fragments arranged by size: 1712 1443 1293 725 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2241 2273 5709 5741 2241 Size: 32 3436 32 2249 Fragments arranged by size: 3436 2249 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2638 2638 Size: 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 3074 1870 Size: 1204 4545 XmnI GAAnn'nnTTC Cuts at: 2015 3878 2015 Size: 1863 3886 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI