################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI KasI KpnI KspI MaeII MfeI MflI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 21 enzymes) # ################################### AcsI 427 AAATTC [GA]^AATT[CT] AlwNI 465 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 427 AAATTC [GA]^AATT[CT] BbvCI 827 CCTCAGC CCTCAGC(-5/-2) BciVI 418 GTATCC GTATCC(6/5) BfaI 286 CTAG C^TAG BpmI 137 CTGGAG CTGGAG(16/14) BsaBI 201 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 70 GAGACC GGTCTC(1/5) BseRI 1056 GAGGAG GAGGAG(10/8) BsgI 548 GTGCAG GTGCAG(16/14) BspMI 637 ACCTGC ACCTGC(4/8) BstF5I 924 CATCC GGATG(2/0) EcoNI 1008 CCTCACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 866 CCTTGG C^C[AT][AT]GG FokI 924 CATCC GGATG(9/13) MaeI 286 CTAG C^TAG MamI 201 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PstI 354 CTGCAG CTGCA^G SfcI 354 CTGCAG C^T[GA][CT]AG StyI 866 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AspHI 324 GAGCTC G[AT]GC[AT]^C 517 GAGCTC BbsI 194 GAAGAC GAAGAC(2/6) 100 GTCTTC BcnI 184 CCCGG CC^[GC]GG 930 CCGGG BpuAI 194 GAAGAC GAAGAC(2/6) 100 GTCTTC BsiHKAI 324 GAGCTC G[AT]GC[AT]^C 517 GAGCTC BsmFI 585 GTCCC GGGAC(10/14) 616 GTCCC BsrDI 254 CATTGC GCAATG(2/0) 651 CATTGC Cac8I 463 GCCAGC GC[ACGT]^[ACGT]GC 485 GCCTGC EarI 503 CTCTTC CTCTTC(1/4) 1062 GAAGAG HapII 185 CCGG C^CGG 930 CCGG HincII 385 GTTGAC GT[CT]^[GA]AC 889 GTCAAC HindII 385 GTTGAC GT[CT]^[GA]AC 889 GTCAAC HpaII 185 CCGG C^CGG 930 CCGG Ksp632I 503 CTCTTC CTCTTC(1/4) 1062 GAAGAG MseI 572 TTAA T^TAA 598 TTAA MspA1I 122 CAGCTG C[AC]G^C[GT]G 882 CAGCTG MspI 185 CCGG C^CGG 930 CCGG NciI 184 CCCGG CC^[GC]GG 930 CCGGG NdeI 449 CATATG CA^TATG 579 CATATG PflMI 401 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 464 CCAGCATCTGG PvuII 122 CAGCTG CAG^CTG 882 CAGCTG SacI 324 GAGCTC GAGCT^C 517 GAGCTC SfaNI 280 GCATC GCATC(5/9) 467 GCATC TaqI 203 TCGA T^CGA 914 TCGA Tru9I 572 TTAA T^TAA 598 TTAA TthHB8I 203 TCGA T^CGA 914 TCGA Van91I 401 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 464 CCAGCATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AluI AvaII BalI BanII BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI HaeIII HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I Tsp509I TspRI