Restriction map for
pFN21AA1432
(Circular) (Six-base) MAPSORT of: pFN21AA1432.seq Check: 3455 from: 1 to: 7078
pFN21AA1432 7078 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4888 278
Size: 53 83 186 936 3352 2468
Fragments arranged by size:
3352 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3034 3420 4421 1937
Size: 1097 386 1001 4594
Fragments arranged by size:
4594 1097 1001 386
Acc65I G'GTAC_C
Cuts at: 4399 4399
Size: 7078
AclI AA'CG_TT
Cuts at: 1563 5205 5578 1563
Size: 3642 373 3063
Fragments arranged by size:
3642 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3124 3461 3999 5141 6525 6793 1632
Size: 1492 337 538 1142 1384 268 1917
Fragments arranged by size:
1917 1492 1384 1142 538 337 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6881
AflIII A'CryG_T
Cuts at: 1203 2276 3276 5977 6954 1203
Size: 1073 1000 2701 977 1327
Fragments arranged by size:
2701 1327 1073 1000 977
AgeI A'CCGG_T
Cuts at: 5909 5909
Size: 7078
AhdI GACnn_n'nnGTC
Cuts at: 2277 5807 2277
Size: 3530 3548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7078
AlwNI CAG_nnn'CTG
Cuts at: 3264 4821 6393 3264
Size: 1557 1572 3949
Fragments arranged by size:
3949 1572 1557
ApaLI G'TGCA_C
Cuts at: 5135 6291 5135
Size: 1156 5922
ApoI r'AATT_y
Cuts at: 1493 2774 3194 3808 4387 4613 4765 6874 1493
Size: 1281 420 614 579 226 152 2109 1697
Fragments arranged by size:
2109 1697 1281 614 579 420 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5632 160
Size: 872 4600 1606
Fragments arranged by size:
4600 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7078
AvaI C'yCGr_G
Cuts at: 1870 1943 3405 3414 4224 4403 1870
Size: 73 1462 9 810 179 4545
Fragments arranged by size:
4545 1462 810 179 73 9
AvrII C'CTAG_G
Cuts at: 2369 2369
Size: 7078
BamHI G'GATC_C
Cuts at: 4408 4775 4408
Size: 367 6711
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3312 3933 4399 5854 618
Size: 325 217 627 1525 621 466 1455 1842
Fragments arranged by size:
1842 1525 1455 627 621 466 325 217
BanII G_rGCy'C
Cuts at: 1391 2697 2742 4059 4247 4397 1391
Size: 1306 45 1317 188 150 4072
Fragments arranged by size:
4072 1317 1306 188 150 45
BbeI G_GCGC'C
Cuts at: 3316 3316
Size: 7078
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2834 4005 928
Size: 971 935 1171 4001
Fragments arranged by size:
4001 1171 971 935
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5269 5303 1903
Size: 34 3332 34 3678
Fragments arranged by size:
3678 3332 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4970 6180 4970
Size: 1210 5868
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7078
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5689 136
Size: 107 122 71 631 605 4017 1525
Fragments arranged by size:
4017 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2583 7073 2583
Size: 4490 2588
BlpI GC'TnA_GC
Cuts at: 2324 3629 4495 2324
Size: 1305 866 4907
Fragments arranged by size:
4907 1305 866
Bme1580I G_kGCm'C
Cuts at: 1790 5139 6295 1790
Size: 3349 1156 2573
Fragments arranged by size:
3349 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7078
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 2647 3108
Size: 416 53 83 399 24 828 402 461
Cuts at: 3108 3160 4809 5767 442
Size: 52 1649 958 1753
Fragments arranged by size:
1753 1649 958 828 461 416 402 399
83 53 52 24
BmtI G_CTAG'C
Cuts at: 1056 2447 1056
Size: 1391 5687
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2595 3324 2593
Size: 2 729 6347
Fragments arranged by size:
6347 729 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2330 2561 2711 3090 3319 3863 4191 4234
Size: 1091 231 150 379 229 544 328 43
Cuts at: 4234 5738 1239
Size: 1504 2579
Fragments arranged by size:
2579 1504 1091 544 379 328 231 229
150 43
Bpu10I CC'TnA_GC
Cuts at: 2907 3105 2907
Size: 198 6880
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3602 5203 6068 6366 3602
Size: 1601 865 298 4314
Fragments arranged by size:
4314 1601 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5741 882
Size: 617 4242 2219
Fragments arranged by size:
4242 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4869 493
Size: 711 3665 2702
Fragments arranged by size:
3665 2702 711
BsaBI GATnn'nnATC
Cuts at: 7072 7072
Size: 7078
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3313 4885 5267 5902
Size: 53 83 186 936 1780 1572 382 635
Cuts at: 5902 275
Size: 1451
Fragments arranged by size:
1780 1572 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5511 5909 6183 6330 6741 5511
Size: 398 274 147 411 5848
Fragments arranged by size:
5848 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3917 3947 1178
Size: 30 165 30 2514 30 4309
Fragments arranged by size:
4309 2514 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2906 4237 4247 4325 4346 1186
Size: 1720 1331 10 78 21 3918
Fragments arranged by size:
3918 1720 1331 78 21 10
BseYI C'CCAG_C
Cuts at: 2403 3360 3364 3484 6281 6777 2403
Size: 957 4 120 2797 496 2704
Fragments arranged by size:
2797 2704 957 496 120 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2130 2358 1163
Size: 573 394 228 5883
Fragments arranged by size:
5883 573 394 228
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4528 5289 6317 1540
Size: 275 186 2527 761 1028 2301
Fragments arranged by size:
2527 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2550 2697 3849 5139 5224 6295 2550
Size: 147 1152 1290 85 1071 3333
Fragments arranged by size:
3333 1290 1152 1071 147 85
BsmI GAATG_Cn'
Cuts at: 3737 4589 4682 3737
Size: 852 93 6133
Fragments arranged by size:
6133 852 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2550 2697 2742 3849 4059 4247 4397
Size: 399 760 147 45 1107 210 188 150
Cuts at: 4397 5139 5224 6295 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1107 1071 760 742 399 210 188
150 147 85 45
BspEI T'CCGG_A
Cuts at: 6741 6741
Size: 7078
BspHI T'CATG_A
Cuts at: 2148 3652 4967 6697 2148
Size: 1504 1315 1730 2529
Fragments arranged by size:
2529 1730 1504 1315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4153 4433 844
Size: 3309 280 3489
Fragments arranged by size:
3489 3309 280
BsrBI CCG'CTC
Cuts at: 1394 1823 4965 1394
Size: 429 3142 3507
Fragments arranged by size:
3507 3142 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5573 5747 65
Size: 1183 4325 174 1396
Fragments arranged by size:
4325 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5722 5909 1700
Size: 110 3912 187 2869
Fragments arranged by size:
3912 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7078
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7078
BssSI C'ACGA_G
Cuts at: 2103 5138 6150 2103
Size: 3035 1012 3031
Fragments arranged by size:
3035 3031 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2641 794
Size: 1847 5231
BstBI TT'CG_AA
Cuts at: 5882 5882
Size: 7078
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7078
BstXI CCAn_nnnn'nTGG
Cuts at: 3862 3862
Size: 7078
BstYI r'GATC_y
Cuts at: 1972 2178 2583 4408 4775 5160 5177 6629 6715
Size: 206 405 1825 367 385 17 1452 86
Cuts at: 6715 6727 7073 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1825 1452 405 385 367 346 206
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2877 2877
Size: 7078
BtgI C'CryG_G
Cuts at: 513 1065 3676 3774 3855 4255 513
Size: 552 2611 98 81 400 3336
Fragments arranged by size:
3336 2611 552 400 98 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6339
Fragments arranged by size:
6339 683 56
BtsI GCAGTG_nn'
Cuts at: 4071 4594 5388 5408 4071
Size: 523 794 20 5741
Fragments arranged by size:
5741 794 523 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7078
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3002 3037 529
Size: 35 2438 35 4570
Fragments arranged by size:
4570 2438 35 35
DraI TTT'AAA
Cuts at: 4113 4382 4113
Size: 269 6809
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6085 809
Size: 507 4769 1802
Fragments arranged by size:
4769 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3467 3864 4525
Size: 54 1425 50 275 18 1637 397 661
Cuts at: 4525 5414 7043 8
Size: 889 1629 43
Fragments arranged by size:
1637 1629 1425 889 661 397 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4525 1537
Size: 275 2713 4090
Fragments arranged by size:
4090 2713 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2369 2555 2801 3437 3496 4152 5008 1353
Size: 1016 186 246 636 59 656 856 3423
Fragments arranged by size:
3423 1016 856 656 636 246 186 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5665 6039 6185 387
Size: 1064 138 4076 374 146 1280
Fragments arranged by size:
4076 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2695 2695
Size: 7078
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2561 2711 3090 3124 3319 3461
Size: 393 698 231 150 379 34 195 142
Cuts at: 3461 3863 3999 4191 4234 5141 5738 6525 6793
Size: 402 136 192 43 907 597 787 268
Cuts at: 6793 1239
Size: 1524
Fragments arranged by size:
1524 907 787 698 597 402 393 379
268 231 195 192 150 142 136 43
34
EcoNI CCTnn'n_nnAGG
Cuts at: 2309 2309
Size: 7078
EcoO109I rG'GnC_Cy
Cuts at: 2314 3025 3340 3875 2314
Size: 711 315 535 5517
Fragments arranged by size:
5517 711 535 315
EcoRI G'AATT_C
Cuts at: 4387 4765 4387
Size: 378 6700
FspI TGC'GCA
Cuts at: 5584 5584
Size: 7078
HaeII r_GCGC'y
Cuts at: 1678 3316 6225 1678
Size: 1638 2909 2531
Fragments arranged by size:
2909 2531 1638
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2130 2162 2263 2295 5719
Size: 32 135 32 558 32 101 32 3424
Cuts at: 5719 5751 5793 5825 1373
Size: 32 42 32 2626
Fragments arranged by size:
3424 2626 558 135 101 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2609 3035 4422 4668 669
Size: 988 281 671 426 1387 246 3079
Fragments arranged by size:
3079 1387 988 671 426 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7078
HpaI GTT'AAC
Cuts at: 4668 4668
Size: 7078
KasI G'GCGC_C
Cuts at: 3312 3312
Size: 7078
KpnI G_GTAC'C
Cuts at: 4403 4403
Size: 7078
MfeI C'AATT_G
Cuts at: 4677 4677
Size: 7078
MluI A'CGCG_T
Cuts at: 6954 6954
Size: 7078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2848 3627 4207 4261 6192 6376 1305
Size: 1543 779 580 54 1931 184 2007
Fragments arranged by size:
2007 1931 1543 779 580 184 54
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3469 3866 10
Size: 54 1425 343 1637 397 3222
Fragments arranged by size:
3222 1637 1425 397 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 3854 3920 5036 5395 5554 518
Size: 648 984 1704 66 1116 359 159 2042
Fragments arranged by size:
2042 1704 1116 984 648 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 2407 2539 2864 2924 3173 3364 3544 3563
Size: 801 132 325 60 249 191 180 19
Cuts at: 3563 4494 5171 6319 6564 1606
Size: 931 677 1148 245 2120
Fragments arranged by size:
2120 1148 931 801 677 325 249 245
191 180 132 60 19
NarI GG'CG_CC
Cuts at: 3313 3313
Size: 7078
NcoI C'CATG_G
Cuts at: 513 1065 3676 3774 3855 4255 513
Size: 552 2611 98 81 400 3336
Fragments arranged by size:
3336 2611 552 400 98 81
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7078
NheI G'CTAG_C
Cuts at: 1052 2443 1052
Size: 1391 5687
NotI GC'GGCC_GC
Cuts at: 4525 4525
Size: 7078
NspI r_CATG'y
Cuts at: 1132 1229 2984 3280 3308 4436 5981 1132
Size: 97 1755 296 28 1128 1545 2229
Fragments arranged by size:
2229 1755 1545 1128 296 97 28
PasI CC'CwG_GG
Cuts at: 1348 4270 1348
Size: 2922 4156
PciI A'CATG_T
Cuts at: 3276 5977 3276
Size: 2701 4377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4066 1108
Size: 2958 4120
PfoI T'CCnGG_A
Cuts at: 2181 4093 2181
Size: 1912 5166
PmeI GTTT'AAAC
Cuts at: 4382 4382
Size: 7078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5147 5179 6660 6692 5147
Size: 32 1481 32 5533
Fragments arranged by size:
5533 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3025 3340 3025
Size: 315 6763
PshAI GACnn'nnGTC
Cuts at: 1613 2810 3030 1613
Size: 1197 220 5661
Fragments arranged by size:
5661 1197 220
PsiI TTA'TAA
Cuts at: 4648 4648
Size: 7078
PstI C_TGCA'G
Cuts at: 830 2805 2968 4137 4430 830
Size: 1975 163 1169 293 3478
Fragments arranged by size:
3478 1975 1169 293 163
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7078
PvuII CAG'CTG
Cuts at: 2539 2864 2924 3173 3364 3544 3563 2539
Size: 325 60 249 191 180 19 6054
Fragments arranged by size:
6054 325 249 191 180 60 19
SacI G_AGCT'C
Cuts at: 2697 2697
Size: 7078
SalI G'TCGA_C
Cuts at: 1936 3033 4420 1936
Size: 1097 1387 4594
Fragments arranged by size:
4594 1387 1097
SapI GCTCTTCn'nnn_
Cuts at: 2555 4152 2555
Size: 1597 5481
SbfI CC_TGCA'GG
Cuts at: 4430 4430
Size: 7078
ScaI AGT'ACT
Cuts at: 2712 5326 7038 2712
Size: 2614 1712 2752
Fragments arranged by size:
2752 2614 1712
SfcI C'TryA_G
Cuts at: 826 1045 2801 2964 4083 4133 4426 5561 6242
Size: 219 1756 163 1119 50 293 1135 681
Cuts at: 6242 6433 826
Size: 191 1471
Fragments arranged by size:
1756 1471 1135 1119 681 293 219 191
163 50
SfoI GGC'GCC
Cuts at: 3314 3314
Size: 7078
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7078
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7078
SmaI CCC'GGG
Cuts at: 1872 3416 4226 4405 1872
Size: 1544 810 179 4545
Fragments arranged by size:
4545 1544 810 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3581 5182 6083 6345 820
Size: 197 926 1638 1601 901 262 1553
Fragments arranged by size:
1638 1601 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4869 493
Size: 4376 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7078
SphI G_CATG'C
Cuts at: 1132 4436 1132
Size: 3304 3774
SspI AAT'ATT
Cuts at: 5 52 5002 5
Size: 47 4950 2081
Fragments arranged by size:
4950 2081 47
StuI AGG'CCT
Cuts at: 1517 3960 1517
Size: 2443 4635
StyI C'CwwG_G
Cuts at: 513 1065 2369 2473 2626 3343 3676 3774 3855
Size: 552 1304 104 153 717 333 98 81
Cuts at: 3855 4255 4517 513
Size: 400 262 3074
Fragments arranged by size:
3074 1304 717 552 400 333 262 153
104 98 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2846 3112 3662 4086 5105 5122 5275
Size: 1255 1266 266 550 424 1019 17 153
Cuts at: 5275 5460 5905 325
Size: 185 445 1498
Fragments arranged by size:
1498 1266 1255 1019 550 445 424 266
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2063 2242 2710
Size: 274 80 33 51 1443 86 179 468
Cuts at: 2710 5324 7036 96
Size: 2614 1712 138
Fragments arranged by size:
2614 1712 1443 468 274 179 138 86
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3917 3949 7038 7070 3917
Size: 32 3089 32 3925
Fragments arranged by size:
3925 3089 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7078
XbaI T'CTAG_A
Cuts at: 4414 4414
Size: 7078
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4277 4277
Size: 7078
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7078
XmaI C'CCGG_G
Cuts at: 1870 3414 4224 4403 1870
Size: 1544 810 179 4545
Fragments arranged by size:
4545 1544 810 179
XmnI GAAnn'nnTTC
Cuts at: 3180 4344 5207 3180
Size: 1164 863 5051
Fragments arranged by size:
5051 1164 863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4886 276
Size: 53 83 186 936 3352 2468
Fragments arranged by size:
3352 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI