(Circular) (Six-base) MAPSORT of: pFN21AE1068.seq Check: 1792 from: 1 to: 6436 pFN21AE1068 6436 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4246 278 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2088 3406 3449 3779 1937 Size: 151 1318 43 330 4594 Fragments arranged by size: 4594 1318 330 151 43 Acc65I G'GTAC_C Cuts at: 3757 3757 Size: 6436 AclI AA'CG_TT Cuts at: 1563 4563 4936 1563 Size: 3000 373 3063 Fragments arranged by size: 3063 3000 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2130 2441 2694 3104 3140 3711 4499 5883 Size: 498 311 253 410 36 571 788 1384 Cuts at: 5883 6151 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 788 571 498 410 311 268 253 36 AfeI AGC'GCT Cuts at: 3593 3593 Size: 6436 AflII C'TTAA_G Cuts at: 820 1017 2165 2897 820 Size: 197 1148 732 4359 Fragments arranged by size: 4359 1148 732 197 AflIII A'CryG_T Cuts at: 1203 2807 3401 5335 6312 1203 Size: 1604 594 1934 977 1327 Fragments arranged by size: 1934 1604 1327 977 594 AgeI A'CCGG_T Cuts at: 5267 5267 Size: 6436 AhdI GACnn_n'nnGTC Cuts at: 5165 5165 Size: 6436 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6436 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6436 AlwNI CAG_nnn'CTG Cuts at: 3112 3163 3562 3715 4179 5751 3112 Size: 51 399 153 464 1572 3797 Fragments arranged by size: 3797 1572 464 399 153 51 ApaI G_GGCC'C Cuts at: 2024 2024 Size: 6436 ApaLI G'TGCA_C Cuts at: 4493 5649 4493 Size: 1156 5280 ApoI r'AATT_y Cuts at: 1493 2100 2242 3745 3971 4123 6232 1493 Size: 607 142 1503 226 152 2109 1697 Fragments arranged by size: 2109 1697 1503 607 226 152 142 AseI AT'TA_AT Cuts at: 160 1032 4990 160 Size: 872 3958 1606 Fragments arranged by size: 3958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 AvaI C'yCGr_G Cuts at: 1870 1943 3761 1870 Size: 73 1818 4545 Fragments arranged by size: 4545 1818 73 BamHI G'GATC_C Cuts at: 3766 4133 3766 Size: 367 6069 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3757 5212 618 Size: 325 217 627 1970 1455 1842 Fragments arranged by size: 1970 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2024 3569 3755 1391 Size: 633 1545 186 4072 Fragments arranged by size: 4072 1545 633 186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2301 3344 928 Size: 971 402 1043 4020 Fragments arranged by size: 4020 1043 971 402 BbvCI CC'TCA_GC Cuts at: 3588 3588 Size: 6436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4627 4661 1903 Size: 34 2690 34 3678 Fragments arranged by size: 3678 2690 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2593 4328 5538 2593 Size: 1735 1210 3491 Fragments arranged by size: 3491 1735 1210 BclI T'GATC_A Cuts at: 1548 2433 1548 Size: 885 5551 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5047 136 Size: 107 122 71 631 605 3375 1525 Fragments arranged by size: 3375 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3087 6431 3087 Size: 3344 3092 BlpI GC'TnA_GC Cuts at: 2379 3853 2379 Size: 1474 4962 Bme1580I G_kGCm'C Cuts at: 1790 2024 3058 4497 5653 1790 Size: 234 1034 1439 1156 2573 Fragments arranged by size: 2573 1439 1156 1034 234 BmgBI CAC'GTC Cuts at: 1318 2014 1318 Size: 696 5740 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2028 3370 4167 Size: 416 53 83 399 24 611 1342 797 Cuts at: 4167 5125 442 Size: 958 1753 Fragments arranged by size: 1753 1342 958 797 611 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2071 2588 2808 3586 5096 1239 Size: 832 517 220 778 1510 2579 Fragments arranged by size: 2579 1510 832 778 517 220 Bpu10I CC'TnA_GC Cuts at: 3588 3588 Size: 6436 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2659 4561 5426 5724 2659 Size: 1902 865 298 3371 Fragments arranged by size: 3371 1902 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2792 5099 882 Size: 617 1293 2307 2219 Fragments arranged by size: 2307 2219 1293 617 BsaAI yAC'GTr Cuts at: 493 1204 4227 493 Size: 711 3023 2702 Fragments arranged by size: 3023 2702 711 BsaBI GATnn'nnATC Cuts at: 6430 6430 Size: 6436 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4243 4625 5260 275 Size: 53 83 186 936 2710 382 635 1451 Fragments arranged by size: 2710 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4869 5267 5541 5688 6099 4869 Size: 398 274 147 411 5206 Fragments arranged by size: 5206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3506 3536 1178 Size: 30 165 30 2103 30 4078 Fragments arranged by size: 4078 2103 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3716 1186 Size: 2530 3906 BseYI C'CCAG_C Cuts at: 3171 3611 5639 6135 3171 Size: 440 2028 496 3472 Fragments arranged by size: 3472 2028 496 440 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5863 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3886 4647 5675 1540 Size: 275 186 1885 761 1028 2301 Fragments arranged by size: 2301 1885 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4497 4582 5653 4497 Size: 85 1071 5280 Fragments arranged by size: 5280 1071 85 BsmI GAATG_Cn' Cuts at: 2480 2571 3947 4040 2480 Size: 91 1376 93 4876 Fragments arranged by size: 4876 1376 93 91 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 3058 3569 3755 4497 4582 5653 Size: 399 234 1034 511 186 742 85 1071 Cuts at: 5653 1391 Size: 2174 Fragments arranged by size: 2174 1071 1034 742 511 399 234 186 85 BspEI T'CCGG_A Cuts at: 6099 6099 Size: 6436 BspHI T'CATG_A Cuts at: 2950 4325 6055 2950 Size: 1375 1730 3331 Fragments arranged by size: 3331 1730 1375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2569 2845 3791 844 Size: 1725 276 946 3489 Fragments arranged by size: 3489 1725 946 276 BsrBI CCG'CTC Cuts at: 1394 1823 4323 1394 Size: 429 2500 3507 Fragments arranged by size: 3507 2500 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2307 4931 5105 65 Size: 1183 1059 2624 174 1396 Fragments arranged by size: 2624 1396 1183 1059 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5080 5267 1700 Size: 110 3270 187 2869 Fragments arranged by size: 3270 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6436 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6436 BssSI C'ACGA_G Cuts at: 4496 5508 4496 Size: 1012 5424 BstAPI GCAn_nnn'nTGC Cuts at: 794 3115 794 Size: 2321 4115 BstBI TT'CG_AA Cuts at: 5240 5240 Size: 6436 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6436 BstYI r'GATC_y Cuts at: 1972 3087 3766 4133 4518 4535 5987 6073 6085 Size: 1115 679 367 385 17 1452 86 12 Cuts at: 6085 6431 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1115 679 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2089 2089 Size: 6436 BtgI C'CryG_G Cuts at: 513 1065 2004 2040 2199 513 Size: 552 939 36 159 4750 Fragments arranged by size: 4750 939 552 159 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5697 Fragments arranged by size: 5697 683 56 BtsI GCAGTG_nn' Cuts at: 3056 3952 4746 4766 3056 Size: 896 794 20 4726 Fragments arranged by size: 4726 896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6436 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6401 DraI TTT'AAA Cuts at: 3740 3740 Size: 6436 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5443 809 Size: 507 4127 1802 Fragments arranged by size: 4127 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2331 3883 4772 Size: 54 1425 50 275 18 501 1552 889 Cuts at: 4772 6401 8 Size: 1629 43 Fragments arranged by size: 1629 1552 1425 889 501 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3883 1537 Size: 275 2071 4090 Fragments arranged by size: 4090 2071 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2460 3687 4366 1353 Size: 1107 1227 679 3423 Fragments arranged by size: 3423 1227 1107 679 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5023 5397 5543 387 Size: 1064 138 435 2999 374 146 1280 Fragments arranged by size: 2999 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2130 2441 2588 2694 2808 3104 Size: 393 439 59 311 147 106 114 296 Cuts at: 3104 3140 3586 3711 4499 5096 5883 6151 1239 Size: 36 446 125 788 597 787 268 1524 Fragments arranged by size: 1524 788 787 597 446 439 393 311 296 268 147 125 114 106 59 36 EcoO109I rG'GnC_Cy Cuts at: 2021 3615 2021 Size: 1594 4842 EcoRI G'AATT_C Cuts at: 2242 3745 4123 2242 Size: 1503 378 4555 Fragments arranged by size: 4555 1503 378 FspI TGC'GCA Cuts at: 4942 4942 Size: 6436 HaeII r_GCGC'y Cuts at: 1678 3154 3595 5583 1678 Size: 1476 441 1988 2531 Fragments arranged by size: 2531 1988 1476 441 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2372 2404 2650 2682 5077 Size: 32 135 32 800 32 246 32 2395 Cuts at: 5077 5109 5151 5183 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2395 800 246 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3780 4026 669 Size: 988 281 1842 246 3079 Fragments arranged by size: 3079 1842 988 281 246 HindIII A'AGCT_T Cuts at: 748 3185 748 Size: 2437 3999 HpaI GTT'AAC Cuts at: 4026 4026 Size: 6436 KpnI G_GTAC'C Cuts at: 3761 3761 Size: 6436 MfeI C'AATT_G Cuts at: 3232 4035 3232 Size: 803 5633 MluI A'CGCG_T Cuts at: 6312 6312 Size: 6436 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2394 3525 3661 3678 5550 5734 1305 Size: 1089 1131 136 17 1872 184 2007 Fragments arranged by size: 2007 1872 1131 1089 184 136 17 MscI TGG'CCA Cuts at: 10 64 1489 1832 2333 10 Size: 54 1425 343 501 4113 Fragments arranged by size: 4113 1425 501 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4394 4753 4912 518 Size: 648 3228 359 159 2042 Fragments arranged by size: 3228 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2042 3852 4529 5677 5922 1606 Size: 436 1810 677 1148 245 2120 Fragments arranged by size: 2120 1810 1148 677 436 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2199 513 Size: 552 939 195 4750 Fragments arranged by size: 4750 939 552 195 NdeI CA'TA_TG Cuts at: 387 3360 387 Size: 2973 3463 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6436 NotI GC'GGCC_GC Cuts at: 3883 3883 Size: 6436 NspI r_CATG'y Cuts at: 1132 1229 2811 3405 3794 5339 1132 Size: 97 1582 594 389 1545 2229 Fragments arranged by size: 2229 1582 1545 594 389 97 PacI TTA_AT'TAA Cuts at: 2322 2322 Size: 6436 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6436 PciI A'CATG_T Cuts at: 2807 3401 5335 2807 Size: 594 1934 3908 Fragments arranged by size: 3908 1934 594 PflMI CCAn_nnn'nTGG Cuts at: 1108 3314 1108 Size: 2206 4230 PfoI T'CCnGG_A Cuts at: 2095 2697 2095 Size: 602 5834 PmeI GTTT'AAAC Cuts at: 3740 3740 Size: 6436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4505 4537 6018 6050 4505 Size: 32 1481 32 4891 Fragments arranged by size: 4891 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2686 1613 Size: 1073 5363 PsiI TTA'TAA Cuts at: 4006 4006 Size: 6436 PspOMI G'GGCC_C Cuts at: 2020 2020 Size: 6436 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2076 2108 2076 Size: 32 6404 PstI C_TGCA'G Cuts at: 830 2252 2695 2842 2941 3788 830 Size: 1422 443 147 99 847 3478 Fragments arranged by size: 3478 1422 847 443 147 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6436 SacII CC_GC'GG Cuts at: 2043 2043 Size: 6436 SalI G'TCGA_C Cuts at: 1936 3778 1936 Size: 1842 4594 SapI GCTCTTCn'nnn_ Cuts at: 2460 2460 Size: 6436 SbfI CC_TGCA'GG Cuts at: 2252 3788 2252 Size: 1536 4900 ScaI AGT'ACT Cuts at: 4684 6396 4684 Size: 1712 4724 SfcI C'TryA_G Cuts at: 826 1045 2248 2462 2691 2838 2937 3784 4919 Size: 219 1203 214 229 147 99 847 1135 Cuts at: 4919 5600 5791 826 Size: 681 191 1471 Fragments arranged by size: 1471 1203 1135 847 681 229 219 214 191 147 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6436 SmaI CCC'GGG Cuts at: 1872 3763 1872 Size: 1891 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2165 2638 2897 4540 5441 5703 Size: 197 926 222 473 259 1643 901 262 Cuts at: 5703 820 Size: 1553 Fragments arranged by size: 1643 1553 926 901 473 262 259 222 197 SnaBI TAC'GTA Cuts at: 493 4227 493 Size: 3734 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6436 SphI G_CATG'C Cuts at: 1132 3794 1132 Size: 2662 3774 SspI AAT'ATT Cuts at: 5 52 2507 4360 5 Size: 47 2455 1853 2081 Fragments arranged by size: 2455 2081 1853 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6436 StyI C'CwwG_G Cuts at: 513 1065 2004 2199 3875 513 Size: 552 939 195 1676 3074 Fragments arranged by size: 3074 1676 939 552 195 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4463 4480 4633 4818 5263 325 Size: 1255 2883 17 153 185 445 1498 Fragments arranged by size: 2883 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4682 6394 96 Size: 274 80 33 51 1443 2705 1712 138 Fragments arranged by size: 2705 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3506 3538 6396 6428 3506 Size: 32 2858 32 3514 Fragments arranged by size: 3514 2858 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3642 1316 Size: 2326 4110 XbaI T'CTAG_A Cuts at: 2779 3772 2779 Size: 993 5443 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6436 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6436 XmaI C'CCGG_G Cuts at: 1870 3761 1870 Size: 1891 4545 XmnI GAAnn'nnTTC Cuts at: 2454 4565 2454 Size: 2111 4325 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4244 276 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PmlI PpuMI PspXI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI