################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI ScaI SfaNI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 171 GTCGAC GT^[AC][GT]AC AfaI 8 GTAC GT^AC AlwI 153 GATCC GGATC(4/5) Aor51HI 85 AGCGCT AGC^GCT AspHI 258 GAGCTC G[AT]GC[AT]^C BalI 62 TGGCCA TGG^CCA BanII 258 GAGCTC G[GA]GC[CT]^C BcnI 130 CCCGG CC^[GC]GG BfaI 211 CTAG C^TAG BmyI 258 GAGCTC G[AGT]GC[ACT]^C BpmI 89 CTCCAG CTGGAG(16/14) BsiEI 173 CGACCG CG[GA][CT]^CG BsiHKAI 258 GAGCTC G[AT]GC[AT]^C BsmI 389 GCATTC GAATGC(1/-1) Bsp1286I 258 GAGCTC G[AGT]GC[ACT]^C BstF5I 372 CATCC GGATG(2/0) BstNI 300 CCTGG CC^[AT]GG BstYI 152 AGATCC [GA]^GATC[CT] Cfr13I 128 GGCCC G^G[ACGT]CC DdeI 11 CTAAG C^T[ACGT]AG DsaI 32 CCACGG C^C[GA][CT]GG EaeI 62 TGGCCA [CT]^GGCC[GA] Eco47III 85 AGCGCT AGC^GCT EcoRII 300 CCTGG ^CC[AT]GG FokI 372 CATCC GGATG(9/13) HgaI 207 GCGTC GACGC(5/10) HincII 171 GTCGAC GT[CT]^[GA]AC HindII 171 GTCGAC GT[CT]^[GA]AC HinfI 186 GATTC G^A[ACGT]TC HphI 267 GGTGA GGTGA(8/7) MaeI 211 CTAG C^TAG MboII 108 GAAGA GAAGA(8/7) MflI 152 AGATCC [GA]^GATC[CT] MluNI 62 TGGCCA TGG^CCA MscI 62 TGGCCA TGG^CCA MspA1I 58 CAGCTG C[AC]G^C[GT]G MvaI 300 CCTGG CC^[AT]GG NciI 130 CCCGG CC^[GC]GG NlaIII 360 CATG CATG^ PstI 277 CTGCAG CTGCA^G PvuII 58 CAGCTG CAG^CTG RsaI 8 GTAC GT^AC SacI 258 GAGCTC GAGCT^C SalI 171 GTCGAC G^TCGAC Sau96I 128 GGCCC G^G[ACGT]CC SexAI 299 ACCTGGT A^CC[AT]GGT SfcI 277 CTGCAG C^T[GA][CT]AG SfiI 285 GGCCTGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 96 CACCGGCG C[GA]^CCGG[CT]G TfiI 186 GATTC G^A[AT]TC Tsp45I 352 GTGAC ^GT[GC]AC XhoII 152 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BcgI 173 CGACCGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 15 GCAGACTGCTCG BglI 64 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 286 GCCTGCGAGGC BsiYI 32 CCACGGGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 91 CCAGCCACCGG BslI 32 CCACGGGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 91 CCAGCCACCGG BsrFI 97 ACCGGC [GA]^CCGG[CT] 125 ACCGGC BssKI 130 CCCGG ^CC[ACGT]GG 300 CCTGG Cfr10I 97 ACCGGC [GA]^CCGG[CT] 125 ACCGGC EcoT14I 68 CCAAGG C^C[AT][AT]GG 365 CCAAGG MaeIII 122 GTTAC ^GT[ACGT]AC 352 GTGAC ScrFI 130 CCCGG CC^[ACGT]GG 300 CCTGG StuI 284 AGGCCT AGG^CCT 293 AGGCCT StyI 68 CCAAGG C^C[AT][AT]GG 365 CCAAGG TaqI 78 TCGA T^CGA 172 TCGA TthHB8I 78 TCGA T^CGA 172 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AccII AciI AluI BbvI BsaJI BstUI Cac8I CfoI DpnII Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboI MnlI MspI MvnI MwoI NdeII Sau3AI TseI