(Circular) (Six-base) MAPSORT of: pFN21AA0984.seq Check: 930 from: 1 to: 6646 pFN21AA0984 6646 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4456 278 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3989 1937 Size: 2052 4594 Acc65I G'GTAC_C Cuts at: 3967 3967 Size: 6646 AclI AA'CG_TT Cuts at: 1563 4773 5146 1563 Size: 3210 373 3063 Fragments arranged by size: 3210 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2326 2381 3113 3558 3889 4709 6093 6361 Size: 694 55 732 445 331 820 1384 268 Cuts at: 6361 1632 Size: 1917 Fragments arranged by size: 1917 1384 820 732 694 445 331 268 55 AflII C'TTAA_G Cuts at: 820 1017 3332 820 Size: 197 2315 4134 Fragments arranged by size: 4134 2315 197 AflIII A'CryG_T Cuts at: 1203 5545 6522 1203 Size: 4342 977 1327 Fragments arranged by size: 4342 1327 977 AgeI A'CCGG_T Cuts at: 5477 5477 Size: 6646 AhdI GACnn_n'nnGTC Cuts at: 3402 5375 3402 Size: 1973 4673 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6646 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6646 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3569 3601 3569 Size: 32 6614 AlwNI CAG_nnn'CTG Cuts at: 2386 2810 2974 4389 5961 2386 Size: 424 164 1415 1572 3071 Fragments arranged by size: 3071 1572 1415 424 164 ApaLI G'TGCA_C Cuts at: 3683 3723 3878 4703 5859 3683 Size: 40 155 825 1156 4470 Fragments arranged by size: 4470 1156 825 155 40 ApoI r'AATT_y Cuts at: 1493 2055 2624 3955 4181 4333 6442 1493 Size: 562 569 1331 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 569 562 226 152 AseI AT'TA_AT Cuts at: 160 1032 5200 160 Size: 872 4168 1606 Fragments arranged by size: 4168 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 AvaI C'yCGr_G Cuts at: 1870 1943 3971 1870 Size: 73 2028 4545 Fragments arranged by size: 4545 2028 73 BamHI G'GATC_C Cuts at: 3976 4343 3976 Size: 367 6279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2643 3967 5422 618 Size: 325 217 627 856 1324 1455 1842 Fragments arranged by size: 1842 1455 1324 856 627 325 217 BanII G_rGCy'C Cuts at: 1391 2439 3637 3678 3965 1391 Size: 1048 1198 41 287 4072 Fragments arranged by size: 4072 1198 1048 287 41 BbeI G_GCGC'C Cuts at: 2647 2647 Size: 6646 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2332 2446 2524 3333 928 Size: 971 433 114 78 809 4241 Fragments arranged by size: 4241 971 809 433 114 78 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2849 2883 3089 3123 4837 Size: 34 50 34 828 34 206 34 1714 Cuts at: 4837 4871 1903 Size: 34 3678 Fragments arranged by size: 3678 1714 828 206 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2383 4538 5748 2383 Size: 2155 1210 3281 Fragments arranged by size: 3281 2155 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6646 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3540 5257 136 Size: 107 122 71 631 605 1868 1717 1525 Fragments arranged by size: 1868 1717 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2517 2978 6641 2517 Size: 461 3663 2522 Fragments arranged by size: 3663 2522 461 BlpI GC'TnA_GC Cuts at: 2115 4063 2115 Size: 1948 4698 Bme1580I G_kGCm'C Cuts at: 1790 3687 3727 3882 4707 5863 1790 Size: 1897 40 155 825 1156 2573 Fragments arranged by size: 2573 1897 1156 825 155 40 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6646 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4377 5335 442 Size: 416 53 83 399 24 2960 958 1753 Fragments arranged by size: 2960 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6646 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3192 5306 1239 Size: 1953 2114 2579 Fragments arranged by size: 2579 2114 1953 Bpu10I CC'TnA_GC Cuts at: 3677 3677 Size: 6646 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4771 5636 5934 4771 Size: 865 298 5483 Fragments arranged by size: 5483 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2340 5309 882 Size: 617 841 2969 2219 Fragments arranged by size: 2969 2219 841 617 BsaAI yAC'GTr Cuts at: 493 1204 4437 493 Size: 711 3233 2702 Fragments arranged by size: 3233 2702 711 BsaBI GATnn'nnATC Cuts at: 6640 6640 Size: 6646 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2644 4453 4835 5470 Size: 53 83 186 936 1111 1809 382 635 Cuts at: 5470 275 Size: 1451 Fragments arranged by size: 1809 1451 1111 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3574 5079 5477 5751 5898 6309 3574 Size: 1505 398 274 147 411 3911 Fragments arranged by size: 3911 1505 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3569 3599 1178 Size: 30 165 30 2166 30 4225 Fragments arranged by size: 4225 2166 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2978 1186 Size: 1792 4854 BseYI C'CCAG_C Cuts at: 2782 3472 3515 3800 5849 6345 2782 Size: 690 43 285 2049 496 3083 Fragments arranged by size: 3083 2049 690 496 285 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2193 1163 Size: 573 457 5616 Fragments arranged by size: 5616 573 457 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3561 4096 4857 5885 1540 Size: 275 186 1560 535 761 1028 2301 Fragments arranged by size: 2301 1560 1028 761 535 275 186 BsiHKAI G_wGCw'C Cuts at: 3678 3687 3727 3882 4707 4792 5863 3678 Size: 9 40 155 825 85 1071 4461 Fragments arranged by size: 4461 1071 825 155 85 40 9 BsmI GAATG_Cn' Cuts at: 3180 3274 3488 4157 4250 3180 Size: 94 214 669 93 5576 Fragments arranged by size: 5576 669 214 94 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3195 3195 Size: 6646 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2439 3637 3678 3687 3727 3882 3965 Size: 399 649 1198 41 9 40 155 83 Cuts at: 3965 4707 4792 5863 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1198 1071 742 649 399 155 85 83 41 40 9 BspEI T'CCGG_A Cuts at: 3574 6309 3574 Size: 2735 3911 BspHI T'CATG_A Cuts at: 4535 6265 4535 Size: 1730 4916 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3124 4001 844 Size: 2280 877 3489 Fragments arranged by size: 3489 2280 877 BsrBI CCG'CTC Cuts at: 1394 1823 4533 1394 Size: 429 2710 3507 Fragments arranged by size: 3507 2710 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2242 2988 5141 5315 65 Size: 1183 994 746 2153 174 1396 Fragments arranged by size: 2153 1396 1183 994 746 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3580 5290 5477 1700 Size: 110 1770 1710 187 2869 Fragments arranged by size: 2869 1770 1710 187 110 BsrGI T'GTAC_A Cuts at: 96 3147 3503 96 Size: 3051 356 3239 Fragments arranged by size: 3239 3051 356 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6646 BssSI C'ACGA_G Cuts at: 4706 5718 4706 Size: 1012 5634 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6646 BstBI TT'CG_AA Cuts at: 5450 5450 Size: 6646 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6646 BstXI CCAn_nnnn'nTGG Cuts at: 3287 3472 3841 3287 Size: 185 369 6092 Fragments arranged by size: 6092 369 185 BstYI r'GATC_y Cuts at: 1972 2517 2978 3976 4343 4728 4745 6197 6283 Size: 545 461 998 367 385 17 1452 86 Cuts at: 6283 6295 6641 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 998 545 461 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2558 2558 Size: 6646 BtgI C'CryG_G Cuts at: 513 1065 2049 2637 2712 3353 513 Size: 552 984 588 75 641 3806 Fragments arranged by size: 3806 984 641 588 552 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5907 Fragments arranged by size: 5907 683 56 BtsI GCAGTG_nn' Cuts at: 2471 2889 3703 4162 4956 4976 2471 Size: 418 814 459 794 20 4141 Fragments arranged by size: 4141 814 794 459 418 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2401 2436 529 Size: 35 1837 35 4739 Fragments arranged by size: 4739 1837 35 35 DraI TTT'AAA Cuts at: 2514 3950 2514 Size: 1436 5210 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5653 809 Size: 507 4337 1802 Fragments arranged by size: 4337 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 4093 4982 Size: 54 1425 50 275 18 274 1989 889 Cuts at: 4982 6611 8 Size: 1629 43 Fragments arranged by size: 1989 1629 1425 889 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4093 1537 Size: 275 2281 4090 Fragments arranged by size: 4090 2281 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2400 2838 4576 1353 Size: 1047 438 1738 3423 Fragments arranged by size: 3423 1738 1047 438 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5233 5607 5753 387 Size: 1064 138 3644 374 146 1280 Fragments arranged by size: 3644 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3676 3676 Size: 6646 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2326 2381 3113 3192 3558 3889 4709 Size: 393 694 55 732 79 366 331 820 Cuts at: 4709 5306 6093 6361 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 820 787 732 694 597 393 366 331 268 79 55 EcoNI CCTnn'n_nnAGG Cuts at: 2651 2651 Size: 6646 EcoO109I rG'GnC_Cy Cuts at: 2071 2071 Size: 6646 EcoRI G'AATT_C Cuts at: 2055 2624 3955 4333 2055 Size: 569 1331 378 4368 Fragments arranged by size: 4368 1331 569 378 EcoRV GAT'ATC Cuts at: 3082 3082 Size: 6646 FspI TGC'GCA Cuts at: 5152 5152 Size: 6646 HaeII r_GCGC'y Cuts at: 1678 2647 5793 1678 Size: 969 3146 2531 Fragments arranged by size: 3146 2531 969 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 3388 3420 3554 Size: 32 135 32 458 32 1326 32 134 Cuts at: 3554 3586 5287 5319 5361 5393 1373 Size: 32 1701 32 42 32 2626 Fragments arranged by size: 2626 1701 1326 458 135 134 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3990 4236 669 Size: 988 281 2052 246 3079 Fragments arranged by size: 3079 2052 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6646 HpaI GTT'AAC Cuts at: 4236 4236 Size: 6646 KasI G'GCGC_C Cuts at: 2643 2643 Size: 6646 KpnI G_GTAC'C Cuts at: 3971 3971 Size: 6646 MfeI C'AATT_G Cuts at: 2735 4245 2735 Size: 1510 5136 MluI A'CGCG_T Cuts at: 6522 6522 Size: 6646 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5760 5944 1305 Size: 4455 184 2007 Fragments arranged by size: 4455 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2106 10 Size: 54 1425 343 274 4550 Fragments arranged by size: 4550 1425 343 274 54 MslI CAynn'nnrTG Cuts at: 518 1166 4604 4963 5122 518 Size: 648 3438 359 159 2042 Fragments arranged by size: 3438 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3515 3665 3700 4062 4739 5887 6132 1606 Size: 1909 150 35 362 677 1148 245 2120 Fragments arranged by size: 2120 1909 1148 677 362 245 150 35 NarI GG'CG_CC Cuts at: 2644 2644 Size: 6646 NcoI C'CATG_G Cuts at: 513 1065 2049 2637 2712 513 Size: 552 984 588 75 4447 Fragments arranged by size: 4447 984 588 552 75 NdeI CA'TA_TG Cuts at: 387 387 Size: 6646 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6646 NotI GC'GGCC_GC Cuts at: 4093 4093 Size: 6646 NspI r_CATG'y Cuts at: 1132 1229 2493 3391 4004 5549 1132 Size: 97 1264 898 613 1545 2229 Fragments arranged by size: 2229 1545 1264 898 613 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6646 PciI A'CATG_T Cuts at: 5545 5545 Size: 6646 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6646 PfoI T'CCnGG_A Cuts at: 3004 3004 Size: 6646 PmeI GTTT'AAAC Cuts at: 3950 3950 Size: 6646 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3569 3601 4715 4747 6228 6260 3569 Size: 32 1114 32 1481 32 3955 Fragments arranged by size: 3955 1481 1114 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2071 2071 Size: 6646 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6646 PsiI TTA'TAA Cuts at: 3215 4216 3215 Size: 1001 5645 PstI C_TGCA'G Cuts at: 830 2931 3616 3664 3998 830 Size: 2101 685 48 334 3478 Fragments arranged by size: 3478 2101 685 334 48 PvuI CG_AT'CG Cuts at: 2001 3561 2001 Size: 1560 5086 PvuII CAG'CTG Cuts at: 3515 3665 3515 Size: 150 6496 SacI G_AGCT'C Cuts at: 3678 3678 Size: 6646 SalI G'TCGA_C Cuts at: 1936 3988 1936 Size: 2052 4594 SbfI CC_TGCA'GG Cuts at: 3998 3998 Size: 6646 ScaI AGT'ACT Cuts at: 3104 4894 6606 3104 Size: 1790 1712 3144 Fragments arranged by size: 3144 1790 1712 SfcI C'TryA_G Cuts at: 826 1045 2089 2629 2927 3612 3660 3858 3994 Size: 219 1044 540 298 685 48 198 136 Cuts at: 3994 5129 5810 6001 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1044 685 681 540 298 219 198 191 136 48 SfoI GGC'GCC Cuts at: 2645 2645 Size: 6646 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6646 SmaI CCC'GGG Cuts at: 1872 3973 1872 Size: 2101 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3332 4750 5651 5913 820 Size: 197 926 1389 1418 901 262 1553 Fragments arranged by size: 1553 1418 1389 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4437 493 Size: 3944 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6646 SphI G_CATG'C Cuts at: 1132 4004 1132 Size: 2872 3774 SspI AAT'ATT Cuts at: 5 52 4570 5 Size: 47 4518 2081 Fragments arranged by size: 4518 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6646 StyI C'CwwG_G Cuts at: 513 1065 2049 2188 2637 2712 3392 3760 4085 Size: 552 984 139 449 75 680 368 325 Cuts at: 4085 513 Size: 3074 Fragments arranged by size: 3074 984 680 552 449 368 325 139 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3702 4673 4690 4843 5028 5473 325 Size: 1255 2122 971 17 153 185 445 1498 Fragments arranged by size: 2122 1498 1255 971 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3102 3147 3503 Size: 274 80 33 51 1443 1125 45 356 Cuts at: 3503 4892 6604 96 Size: 1389 1712 138 Fragments arranged by size: 1712 1443 1389 1125 356 274 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6606 6638 6606 Size: 32 6614 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6646 XbaI T'CTAG_A Cuts at: 3982 3982 Size: 6646 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6646 XmaI C'CCGG_G Cuts at: 1870 3971 1870 Size: 2101 4545 XmnI GAAnn'nnTTC Cuts at: 4775 4775 Size: 6646 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4454 276 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI