Restriction map for
pFN21AA0984
(Linear) (Six-base) MAPSORT of: KIAA0984.seq Check: 496 from: 1 to: 1941
KIAA0984.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 376 1108 1553 1884 1941
Size: 321 55 732 445 331 57
Fragments arranged by size:
732 445 331 321 57 55
AflII C'TTAA_G
Cuts at: 0 1327 1941
Size: 1327 614
AhdI GACnn_n'nnGTC
Cuts at: 0 1397 1941
Size: 1397 544
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1564 1596 1941
Size: 1564 32 345
Fragments arranged by size:
1564 345 32
AlwNI CAG_nnn'CTG
Cuts at: 0 381 805 969 1941
Size: 381 424 164 972
Fragments arranged by size:
972 424 381 164
ApaLI G'TGCA_C
Cuts at: 0 1678 1718 1873 1941
Size: 1678 40 155 68
Fragments arranged by size:
1678 155 68 40
ApoI r'AATT_y
Cuts at: 0 50 619 1941
Size: 50 569 1322
Fragments arranged by size:
1322 569 50
BanI G'GyrC_C
Cuts at: 0 638 1941
Size: 638 1303
BanII G_rGCy'C
Cuts at: 0 434 1632 1673 1941
Size: 434 1198 41 268
Fragments arranged by size:
1198 434 268 41
BbeI G_GCGC'C
Cuts at: 0 642 1941
Size: 642 1299
BbsI GAAGACnn'nnnn_
Cuts at: 0 327 441 519 1328 1941
Size: 327 114 78 809 613
Fragments arranged by size:
809 613 327 114 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 844 878 1084 1118 1941
Size: 844 34 206 34 823
Fragments arranged by size:
844 823 206 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 378 1941
Size: 378 1563
BglI GCCn_nnn'nGGC
Cuts at: 0 1535 1941
Size: 1535 406
BglII A'GATC_T
Cuts at: 0 512 973 1941
Size: 512 461 968
Fragments arranged by size:
968 512 461
BlpI GC'TnA_GC
Cuts at: 0 110 1941
Size: 110 1831
Bme1580I G_kGCm'C
Cuts at: 0 1682 1722 1877 1941
Size: 1682 40 155 64
Fragments arranged by size:
1682 155 64 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1187 1941
Size: 1187 754
Bpu10I CC'TnA_GC
Cuts at: 0 1672 1941
Size: 1672 269
BsaI GGTCTCn'nnnn_
Cuts at: 0 335 1941
Size: 335 1606
BsaHI Gr'CG_yC
Cuts at: 0 639 1941
Size: 639 1302
BsaWI w'CCGG_w
Cuts at: 0 1569 1941
Size: 1569 372
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1564 1594 1941
Size: 1564 30 347
Fragments arranged by size:
1564 347 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 973 1941
Size: 973 968
BseYI C'CCAG_C
Cuts at: 0 777 1467 1510 1795 1941
Size: 777 690 43 285 146
Fragments arranged by size:
777 690 285 146 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1941
Size: 188 1753
BsiEI CG_ry'CG
Cuts at: 0 1556 1941
Size: 1556 385
BsiHKAI G_wGCw'C
Cuts at: 0 1673 1682 1722 1877 1941
Size: 1673 9 40 155 64
Fragments arranged by size:
1673 155 64 40 9
BsmI GAATG_Cn'
Cuts at: 0 1175 1269 1483 1941
Size: 1175 94 214 458
Fragments arranged by size:
1175 458 214 94
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1190 1941
Size: 1190 751
Bsp1286I G_dGCh'C
Cuts at: 0 434 1632 1673 1682 1722 1877 1941
Size: 434 1198 41 9 40 155 64
Fragments arranged by size:
1198 434 155 64 41 40 9
BspEI T'CCGG_A
Cuts at: 0 1569 1941
Size: 1569 372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1119 1941
Size: 1119 822
BsrDI GCAATG_nn'
Cuts at: 0 237 983 1941
Size: 237 746 958
Fragments arranged by size:
958 746 237
BsrFI r'CCGG_y
Cuts at: 0 1575 1941
Size: 1575 366
BsrGI T'GTAC_A
Cuts at: 0 1142 1498 1941
Size: 1142 356 443
Fragments arranged by size:
1142 443 356
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1282 1467 1836 1941
Size: 1282 185 369 105
Fragments arranged by size:
1282 369 185 105
BstYI r'GATC_y
Cuts at: 0 512 973 1941
Size: 512 461 968
Fragments arranged by size:
968 512 461
Bsu36I CC'TnA_GG
Cuts at: 0 553 1941
Size: 553 1388
BtgI C'CryG_G
Cuts at: 0 44 632 707 1348 1941
Size: 44 588 75 641 593
Fragments arranged by size:
641 593 588 75 44
BtsI GCAGTG_nn'
Cuts at: 0 466 884 1698 1941
Size: 466 418 814 243
Fragments arranged by size:
814 466 418 243
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 396 431 1941
Size: 396 35 1510
Fragments arranged by size:
1510 396 35
DraI TTT'AAA
Cuts at: 0 509 1941
Size: 509 1432
EaeI y'GGCC_r
Cuts at: 0 99 1941
Size: 99 1842
EarI CTCTTCn'nnn_
Cuts at: 0 395 833 1941
Size: 395 438 1108
Fragments arranged by size:
1108 438 395
EcoICRI GAG'CTC
Cuts at: 0 1671 1941
Size: 1671 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 376 1108 1187 1553 1884 1941
Size: 321 55 732 79 366 331 57
Fragments arranged by size:
732 366 331 321 79 57 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 646 1941
Size: 646 1295
EcoO109I rG'GnC_Cy
Cuts at: 0 66 1941
Size: 66 1875
EcoRI G'AATT_C
Cuts at: 0 50 619 1941
Size: 50 569 1322
Fragments arranged by size:
1322 569 50
EcoRV GAT'ATC
Cuts at: 0 1077 1941
Size: 1077 864
HaeII r_GCGC'y
Cuts at: 0 642 1941
Size: 642 1299
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 1383 1415 1549 1581 1941
Size: 25 32 1326 32 134 32 360
Fragments arranged by size:
1326 360 134 32 32 32 25
KasI G'GCGC_C
Cuts at: 0 638 1941
Size: 638 1303
MfeI C'AATT_G
Cuts at: 0 730 1941
Size: 730 1211
MscI TGG'CCA
Cuts at: 0 101 1941
Size: 101 1840
MspA1I CmG'CkG
Cuts at: 0 1510 1660 1695 1941
Size: 1510 150 35 246
Fragments arranged by size:
1510 246 150 35
NarI GG'CG_CC
Cuts at: 0 639 1941
Size: 639 1302
NcoI C'CATG_G
Cuts at: 0 44 632 707 1941
Size: 44 588 75 1234
Fragments arranged by size:
1234 588 75 44
NspI r_CATG'y
Cuts at: 0 488 1386 1941
Size: 488 898 555
Fragments arranged by size:
898 555 488
PfoI T'CCnGG_A
Cuts at: 0 999 1941
Size: 999 942
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1564 1596 1941
Size: 1564 32 345
Fragments arranged by size:
1564 345 32
PpuMI rG'GwC_Cy
Cuts at: 0 66 1941
Size: 66 1875
PsiI TTA'TAA
Cuts at: 0 1210 1941
Size: 1210 731
PstI C_TGCA'G
Cuts at: 0 926 1611 1659 1941
Size: 926 685 48 282
Fragments arranged by size:
926 685 282 48
PvuI CG_AT'CG
Cuts at: 0 1556 1941
Size: 1556 385
PvuII CAG'CTG
Cuts at: 0 1510 1660 1941
Size: 1510 150 281
Fragments arranged by size:
1510 281 150
SacI G_AGCT'C
Cuts at: 0 1673 1941
Size: 1673 268
ScaI AGT'ACT
Cuts at: 0 1099 1941
Size: 1099 842
SfcI C'TryA_G
Cuts at: 0 84 624 922 1607 1655 1853 1941
Size: 84 540 298 685 48 198 88
Fragments arranged by size:
685 540 298 198 88 84 48
SfoI GGC'GCC
Cuts at: 0 640 1941
Size: 640 1301
SmlI C'TyrA_G
Cuts at: 0 1327 1941
Size: 1327 614
StyI C'CwwG_G
Cuts at: 0 44 183 632 707 1387 1755 1941
Size: 44 139 449 75 680 368 186
Fragments arranged by size:
680 449 368 186 139 75 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1697 1941
Size: 1697 244
TatI w'GTAC_w
Cuts at: 0 1097 1142 1498 1941
Size: 1097 45 356 443
Fragments arranged by size:
1097 443 356 45
Enzymes that do cut:
AcuI AflII AhdI AloI AlwNI ApaLI ApoI BanI
BanII BbeI BbsI BcgI BciVI BglI BglII BlpI
Bme1580I BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I BtgI
BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I KasI MfeI MscI
MspA1I NarI NcoI NspI PfoI PpiI PpuMI PsiI
PstI PvuI PvuII SacI ScaI SfcI SfoI SmlI
StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AleI AlfI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BclI BfrBI BmgBI BmrI BmtI
BplI BpuEI BsaAI BsaBI BsiWI BspHI BsrBI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraIII
DrdI EagI EciI FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MluI MmeI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PshAI PspOMI PspXI PsrI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI