Restriction map for
pFN21AA0746
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvrII BanII BcgI BclI
BfrI BglI BglII BlnI BmyI
BsaBI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I Bsp1407I BspEI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FbaI FseI
KpnI KspI MamI MluI MroI
NaeI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 61 CGCG CG^CG
AflIII 2136 ACATGT A^C[GA][CT]GT
AviII 2028 TGCGCA TGC^GCA
BamHI 1716 GGATCC G^GATCC
BanI 1699 GGCGCC G^G[CT][GA]CC
BbeI 1699 GGCGCC GGCGC^C
BbrPI 2930 CACGTG CAC^GTG
BbvCI 895 GCTGAGG CCTCAGC(-5/-2)
BciVI 1243 GGATAC GTATCC(6/5)
BlpI 1612 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1612 GCTCAGC GC^T[ACGT]AGC
BsaAI 2930 CACGTG [CT]AC^GT[GA]
BsmBI 1708 GAGACG CGTCTC(1/5)
BspDI 2368 ATCGAT AT^CGAT
BspLU11I 2136 ACATGT A^CATGT
BstAPI 1476 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 61 CGCG CG^CG
CelII 1612 GCTCAGC GC^T[ACGT]AGC
ClaI 2368 ATCGAT AT^CGAT
DraII 2050 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 517 GAAGAG CTCTTC(1/4)
EcoO109I 2050 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 616 GATATC GAT^ATC
FspI 2028 TGCGCA TGC^GCA
HaeII 1699 GGCGCC [GA]GCGC^[CT]
HindIII 2216 AAGCTT A^AGCTT
HpaI 2326 GTTAAC GTT^AAC
KasI 1699 GGCGCC G^GCGCC
Ksp632I 517 GAAGAG CTCTTC(1/4)
MfeI 1679 CAATTG C^AATTG
MunI 1679 CAATTG C^AATTG
MvnI 61 CGCG CG^CG
NarI 1699 GGCGCC GG^CGCC
NdeI 1499 CATATG CA^TATG
PmaCI 2930 CACGTG CAC^GTG
PmlI 2930 CACGTG CAC^GTG
PpuMI 2050 GGGACCT [GA]G^G[AT]CC[CT]
SfoI 1699 GGCGCC GGC^GCC
SphI 961 GCATGC GCATG^C
SspI 2012 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 377 GACGCC G[GA]^CG[CT]C
1699 GGCGCC
BcnI 702 CCGGG CC^[GC]GG
2922 CCCGG
BsaHI 377 GACGCC G[GA]^CG[CT]C
1699 GGCGCC
BsaI 1008 GAGACC GGTCTC(1/5)
2402 GAGACC
BseRI 2290 GAGGAG GAGGAG(10/8)
2644 GAGGAG
BsmFI 2050 GGGAC GGGAC(10/14)
1213 GTCCC
EcoT22I 541 ATGCAT ATGCA^T
923 ATGCAT
FauI 165 CCCGC CCCGC(4/6)
823 CCCGC
HapII 702 CCGG C^CGG
2923 CCGG
HgaI 377 GACGC GACGC(5/10)
1933 GCGTC
Hin1I 377 GACGCC G[GA]^CG[CT]C
1699 GGCGCC
HincII 472 GTTGAC GT[CT]^[GA]AC
2326 GTTAAC
HindII 472 GTTGAC GT[CT]^[GA]AC
2326 GTTAAC
HpaII 702 CCGG C^CGG
2923 CCGG
HphI 2008 GGTGA GGTGA(8/7)
1194 TCACC
MaeII 2848 ACGT A^CGT
2931 ACGT
MspI 702 CCGG C^CGG
2923 CCGG
NciI 702 CCGGG CC^[GC]GG
2922 CCCGG
NsiI 541 ATGCAT ATGCA^T
923 ATGCAT
PflMI 381 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2547 CCATATCTTGG
ScaI 797 AGTACT AGT^ACT
1895 AGTACT
StuI 2181 AGGCCT AGG^CCT
2828 AGGCCT
Van91I 381 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2547 CCATATCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaI AvaII BalI
BbsI BbvI BfaI BpmI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsoBI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI ItaI MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII