(Circular) (Six-base) MAPSORT of: pFN21AA0746.seq Check: 9896 from: 1 to: 7642 pFN21AA0746 7642 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5452 278 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4985 1937 Size: 3048 4594 Acc65I G'GTAC_C Cuts at: 4963 4963 Size: 7642 AclI AA'CG_TT Cuts at: 1563 5769 6142 1563 Size: 4206 373 3063 Fragments arranged by size: 4206 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2819 4642 5705 7089 7357 1632 Size: 1187 1823 1063 1384 268 1917 Fragments arranged by size: 1917 1823 1384 1187 1063 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7445 AflIII A'CryG_T Cuts at: 1203 4141 6541 7518 1203 Size: 2938 2400 977 1327 Fragments arranged by size: 2938 2400 1327 977 AgeI A'CCGG_T Cuts at: 6473 6473 Size: 7642 AhdI GACnn_n'nnGTC Cuts at: 6371 6371 Size: 7642 AleI CACnn'nnGTG Cuts at: 1166 4290 1166 Size: 3124 4518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7642 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3285 3317 3285 Size: 32 7610 AlwNI CAG_nnn'CTG Cuts at: 2100 3243 3640 4253 4618 4665 4847 5385 6957 Size: 1143 397 613 365 47 182 538 1572 Cuts at: 6957 2100 Size: 2785 Fragments arranged by size: 2785 1572 1143 613 538 397 365 182 47 ApaLI G'TGCA_C Cuts at: 5699 6855 5699 Size: 1156 6486 ApoI r'AATT_y Cuts at: 1493 2585 3869 4276 4951 5177 5329 7438 1493 Size: 1092 1284 407 675 226 152 2109 1697 Fragments arranged by size: 2109 1697 1284 1092 675 407 226 152 AseI AT'TA_AT Cuts at: 160 1032 6196 160 Size: 872 5164 1606 Fragments arranged by size: 5164 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 AvaI C'yCGr_G Cuts at: 1870 1943 2347 3306 3600 4967 1870 Size: 73 404 959 294 1367 4545 Fragments arranged by size: 4545 1367 959 404 294 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4312 4345 4312 Size: 33 7609 BamHI G'GATC_C Cuts at: 3721 4972 5339 3721 Size: 1251 367 6024 Fragments arranged by size: 6024 1251 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3704 4963 6418 618 Size: 325 217 627 1917 1259 1455 1842 Fragments arranged by size: 1917 1842 1455 1259 627 325 217 BanII G_rGCy'C Cuts at: 1391 4961 1391 Size: 3570 4072 BbeI G_GCGC'C Cuts at: 3708 3708 Size: 7642 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2571 3188 3360 928 Size: 971 672 617 172 5210 Fragments arranged by size: 5210 971 672 617 172 BbvCI CC'TCA_GC Cuts at: 2901 2901 Size: 7642 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5833 5867 1903 Size: 34 3896 34 3678 Fragments arranged by size: 3896 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3242 5534 6744 3242 Size: 2292 1210 4140 Fragments arranged by size: 4140 2292 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7642 BfrBI ATG'CAT Cuts at: 2548 2930 2548 Size: 382 7260 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6253 136 Size: 107 122 71 631 605 4581 1525 Fragments arranged by size: 4581 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7637 7637 Size: 7642 BlpI GC'TnA_GC Cuts at: 3618 5059 3618 Size: 1441 6201 Bme1580I G_kGCm'C Cuts at: 1790 5703 6859 1790 Size: 3913 1156 2573 Fragments arranged by size: 3913 2573 1156 BmgBI CAC'GTC Cuts at: 1318 4854 1318 Size: 3536 4106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2396 3433 5373 Size: 416 53 83 399 24 979 1037 1940 Cuts at: 5373 6331 442 Size: 958 1753 Fragments arranged by size: 1940 1753 1037 979 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7642 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2296 2390 2981 3110 3731 4002 4115 4469 Size: 1057 94 591 129 621 271 113 354 Cuts at: 4469 4595 4654 4863 6302 1239 Size: 126 59 209 1439 2579 Fragments arranged by size: 2579 1439 1057 621 591 354 271 209 129 126 113 94 59 Bpu10I CC'TnA_GC Cuts at: 2901 2901 Size: 7642 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2231 2873 3527 5767 6632 6930 2231 Size: 642 654 2240 865 298 2943 Fragments arranged by size: 2943 2240 865 654 642 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3007 4401 6305 882 Size: 617 1508 1394 1904 2219 Fragments arranged by size: 2219 1904 1508 1394 617 BsaAI yAC'GTr Cuts at: 493 1204 4937 5433 493 Size: 711 3733 496 2702 Fragments arranged by size: 3733 2702 711 496 BsaBI GATnn'nnATC Cuts at: 7636 7636 Size: 7642 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2383 3705 5449 5831 Size: 53 83 186 936 850 1322 1744 382 Cuts at: 5831 6466 275 Size: 635 1451 Fragments arranged by size: 1744 1451 1322 936 850 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6075 6473 6747 6894 7305 6075 Size: 398 274 147 411 6412 Fragments arranged by size: 6412 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4627 4657 1178 Size: 30 165 30 3224 30 4163 Fragments arranged by size: 4163 3224 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4310 4664 1186 Size: 3124 354 4164 Fragments arranged by size: 4164 3124 354 BseYI C'CCAG_C Cuts at: 2735 3068 6845 7341 2735 Size: 333 3777 496 3036 Fragments arranged by size: 3777 3036 496 333 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2807 3046 4481 4673 4703 4802 1163 Size: 573 1071 239 1435 192 30 99 4003 Fragments arranged by size: 4003 1435 1071 573 239 192 99 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5092 5853 6881 1540 Size: 275 186 3091 761 1028 2301 Fragments arranged by size: 3091 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5703 5788 6859 5703 Size: 85 1071 6486 Fragments arranged by size: 6486 1071 85 BsmI GAATG_Cn' Cuts at: 5153 5246 5153 Size: 93 7549 BsmBI CGTCTCn'nnnn_ Cuts at: 3707 3707 Size: 7642 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4961 5703 5788 6859 1391 Size: 399 3171 742 85 1071 2174 Fragments arranged by size: 3171 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7305 7305 Size: 7642 BspHI T'CATG_A Cuts at: 5531 7261 5531 Size: 1730 5912 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2751 2994 4695 4997 844 Size: 1907 243 1701 302 3489 Fragments arranged by size: 3489 1907 1701 302 243 BsrBI CCG'CTC Cuts at: 1394 1823 5529 1394 Size: 429 3706 3507 Fragments arranged by size: 3706 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3083 3453 3613 6137 6311 65 Size: 1183 1835 370 160 2524 174 1396 Fragments arranged by size: 2524 1835 1396 1183 370 174 160 BsrFI r'CCGG_y Cuts at: 1700 1810 6286 6473 1700 Size: 110 4476 187 2869 Fragments arranged by size: 4476 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7642 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7642 BssSI C'ACGA_G Cuts at: 5702 6714 5702 Size: 1012 6630 BstAPI GCAn_nnn'nTGC Cuts at: 794 3487 794 Size: 2693 4949 BstBI TT'CG_AA Cuts at: 6446 6446 Size: 7642 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7642 BstYI r'GATC_y Cuts at: 1972 3097 3721 4111 4972 5339 5724 5741 7193 Size: 1125 624 390 861 367 385 17 1452 Cuts at: 7193 7279 7291 7637 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1125 861 624 390 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6151 Fragments arranged by size: 6151 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6903 Fragments arranged by size: 6903 683 56 BtsI GCAGTG_nn' Cuts at: 2061 3485 4845 4857 5158 5952 5972 2061 Size: 1424 1360 12 301 794 20 3731 Fragments arranged by size: 3731 1424 1360 794 301 20 12 ClaI AT'CG_AT Cuts at: 1554 4374 1554 Size: 2820 4822 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2071 2106 2440 2475 2644 2679 529 Size: 35 1507 35 334 35 169 35 5492 Fragments arranged by size: 5492 1507 334 169 35 35 35 35 DraI TTT'AAA Cuts at: 4946 4946 Size: 7642 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6649 809 Size: 507 5333 1802 Fragments arranged by size: 5333 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3663 4311 4423 Size: 54 1425 50 275 18 1833 648 112 Cuts at: 4423 5089 5978 7607 8 Size: 666 889 1629 43 Fragments arranged by size: 1833 1629 1425 889 666 648 275 112 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5089 1537 Size: 275 3277 4090 Fragments arranged by size: 4090 3277 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2517 5572 1353 Size: 1164 3055 3423 Fragments arranged by size: 3423 3055 1164 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6229 6603 6749 387 Size: 1064 138 4640 374 146 1280 Fragments arranged by size: 4640 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2296 2390 2819 2981 3110 3731 4002 Size: 393 664 94 429 162 129 621 271 Cuts at: 4002 4115 4469 4595 4642 4654 4863 5705 6302 Size: 113 354 126 47 12 209 842 597 Cuts at: 6302 7089 7357 1239 Size: 787 268 1524 Fragments arranged by size: 1524 842 787 664 621 597 429 393 354 271 268 209 162 129 126 113 94 47 12 EcoO109I rG'GnC_Cy Cuts at: 4056 4056 Size: 7642 EcoRI G'AATT_C Cuts at: 4951 5329 4951 Size: 378 7264 EcoRV GAT'ATC Cuts at: 2623 2623 Size: 7642 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2225 2225 Size: 7642 FspI TGC'GCA Cuts at: 4035 6148 4035 Size: 2113 5529 HaeII r_GCGC'y Cuts at: 1678 3708 6789 1678 Size: 2030 3081 2531 Fragments arranged by size: 3081 2531 2030 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2025 2057 2607 2639 2827 Size: 32 135 32 453 32 550 32 188 Cuts at: 2827 2859 3350 3382 3706 3738 4478 4510 6283 Size: 32 491 32 324 32 740 32 1773 Cuts at: 6283 6315 6357 6389 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1773 740 550 491 453 324 188 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2479 4333 4986 5232 669 Size: 988 281 541 1854 653 246 3079 Fragments arranged by size: 3079 1854 988 653 541 281 246 HindIII A'AGCT_T Cuts at: 748 4221 748 Size: 3473 4169 HpaI GTT'AAC Cuts at: 4333 5232 4333 Size: 899 6743 KasI G'GCGC_C Cuts at: 3704 3704 Size: 7642 KpnI G_GTAC'C Cuts at: 4967 4967 Size: 7642 MfeI C'AATT_G Cuts at: 3684 5241 3684 Size: 1557 6085 MluI A'CGCG_T Cuts at: 7518 7518 Size: 7642 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3322 6756 6940 1305 Size: 2017 3434 184 2007 Fragments arranged by size: 3434 2017 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3665 4313 4425 10 Size: 54 1425 343 1833 648 112 3227 Fragments arranged by size: 3227 1833 1425 648 343 112 54 MslI CAynn'nnrTG Cuts at: 518 1166 2724 3966 4290 5600 5959 6118 518 Size: 648 1558 1242 324 1310 359 159 2042 Fragments arranged by size: 2042 1558 1310 1242 648 359 324 159 MspA1I CmG'CkG Cuts at: 1606 2735 3240 3316 4871 4883 5058 5735 6883 Size: 1129 505 76 1555 12 175 677 1148 Cuts at: 6883 7128 1606 Size: 245 2120 Fragments arranged by size: 2120 1555 1148 1129 677 505 245 175 76 12 NarI GG'CG_CC Cuts at: 3705 3705 Size: 7642 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6151 Fragments arranged by size: 6151 939 552 NdeI CA'TA_TG Cuts at: 387 3505 387 Size: 3118 4524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7642 NotI GC'GGCC_GC Cuts at: 5089 5089 Size: 7642 NsiI A_TGCA'T Cuts at: 2550 2932 2550 Size: 382 7260 NspI r_CATG'y Cuts at: 1132 1229 2970 3328 4145 5000 6545 1132 Size: 97 1741 358 817 855 1545 2229 Fragments arranged by size: 2229 1741 1545 855 817 358 97 PasI CC'CwG_GG Cuts at: 1348 3039 4498 1348 Size: 1691 1459 4492 Fragments arranged by size: 4492 1691 1459 PciI A'CATG_T Cuts at: 4141 6541 4141 Size: 2400 5242 PflMI CCAn_nnn'nTGG Cuts at: 1108 2392 4558 1108 Size: 1284 2166 4192 Fragments arranged by size: 4192 2166 1284 PfoI T'CCnGG_A Cuts at: 2706 3988 4611 2706 Size: 1282 623 5737 Fragments arranged by size: 5737 1282 623 PmeI GTTT'AAAC Cuts at: 4946 4946 Size: 7642 PmlI CAC'GTG Cuts at: 4937 4937 Size: 7642 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5711 5743 7224 7256 5711 Size: 32 1481 32 6097 Fragments arranged by size: 6097 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4056 4056 Size: 7642 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7642 PsiI TTA'TAA Cuts at: 2724 5212 2724 Size: 2488 5154 PstI C_TGCA'G Cuts at: 830 2056 2163 2938 3321 4888 4994 830 Size: 1226 107 775 383 1567 106 3478 Fragments arranged by size: 3478 1567 1226 775 383 107 106 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7642 PvuII CAG'CTG Cuts at: 2735 3240 3316 4871 4883 2735 Size: 505 76 1555 12 5494 Fragments arranged by size: 5494 1555 505 76 12 SalI G'TCGA_C Cuts at: 1936 4984 1936 Size: 3048 4594 SbfI CC_TGCA'GG Cuts at: 4994 4994 Size: 7642 ScaI AGT'ACT Cuts at: 2804 3902 5890 7602 2804 Size: 1098 1988 1712 2844 Fragments arranged by size: 2844 1988 1712 1098 SfcI C'TryA_G Cuts at: 826 1045 2052 2136 2159 2934 3317 3445 4884 Size: 219 1007 84 23 775 383 128 1439 Cuts at: 4884 4990 6125 6806 6997 826 Size: 106 1135 681 191 1471 Fragments arranged by size: 1471 1439 1135 1007 775 681 383 219 191 128 106 84 23 SfoI GGC'GCC Cuts at: 3706 3706 Size: 7642 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7642 SmaI CCC'GGG Cuts at: 1872 4969 1872 Size: 3097 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2210 2852 3542 5746 6647 6909 Size: 197 926 267 642 690 2204 901 262 Cuts at: 6909 820 Size: 1553 Fragments arranged by size: 2204 1553 926 901 690 642 267 262 197 SnaBI TAC'GTA Cuts at: 493 5433 493 Size: 4940 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7642 SphI G_CATG'C Cuts at: 1132 2970 5000 1132 Size: 1838 2030 3774 Fragments arranged by size: 3774 2030 1838 SspI AAT'ATT Cuts at: 5 52 4019 5566 5 Size: 47 3967 1547 2081 Fragments arranged by size: 3967 2081 1547 47 StuI AGG'CCT Cuts at: 1517 4188 4835 1517 Size: 2671 647 4324 Fragments arranged by size: 4324 2671 647 StyI C'CwwG_G Cuts at: 513 1065 2004 3113 3699 3822 4671 5081 513 Size: 552 939 1109 586 123 849 410 3074 Fragments arranged by size: 3074 1109 939 849 586 552 410 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4316 5669 5686 5839 6024 6469 325 Size: 1255 2736 1353 17 153 185 445 1498 Fragments arranged by size: 2736 1498 1353 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2469 2802 3900 Size: 274 80 33 51 1443 492 333 1098 Cuts at: 3900 5888 7600 96 Size: 1988 1712 138 Fragments arranged by size: 1988 1712 1443 1098 492 333 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4248 4280 4849 4881 7602 7634 4248 Size: 32 569 32 2721 32 4256 Fragments arranged by size: 4256 2721 569 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7642 XbaI T'CTAG_A Cuts at: 4978 4978 Size: 7642 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3005 3706 4321 3005 Size: 701 615 6326 Fragments arranged by size: 6326 701 615 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7642 XmaI C'CCGG_G Cuts at: 1870 4967 1870 Size: 3097 4545 XmnI GAAnn'nnTTC Cuts at: 5771 5771 Size: 7642 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5450 276 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI