Restriction map for
pFN21AA0746
(Linear) (Six-base) MAPSORT of: KIAA0746.seq Check: 887 from: 1 to: 2937
KIAA0746.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 2637 2937
Size: 814 1823 300
Fragments arranged by size:
1823 814 300
AflIII A'CryG_T
Cuts at: 0 2136 2937
Size: 2136 801
AleI CACnn'nnGTG
Cuts at: 0 2285 2937
Size: 2285 652
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1280 1312 2937
Size: 1280 32 1625
Fragments arranged by size:
1625 1280 32
AlwNI CAG_nnn'CTG
Cuts at: 0 95 1238 1635 2248 2613 2660 2842 2937
Size: 95 1143 397 613 365 47 182 95
Fragments arranged by size:
1143 613 397 365 182 95 95 47
ApoI r'AATT_y
Cuts at: 0 580 1864 2271 2937
Size: 580 1284 407 666
Fragments arranged by size:
1284 666 580 407
AvaI C'yCGr_G
Cuts at: 0 342 1301 1595 2937
Size: 342 959 294 1342
Fragments arranged by size:
1342 959 342 294
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2307 2340 2937
Size: 2307 33 597
Fragments arranged by size:
2307 597 33
BamHI G'GATC_C
Cuts at: 0 1716 2937
Size: 1716 1221
BanI G'GyrC_C
Cuts at: 0 1699 2937
Size: 1699 1238
BbeI G_GCGC'C
Cuts at: 0 1703 2937
Size: 1703 1234
BbsI GAAGACnn'nnnn_
Cuts at: 0 566 1183 1355 2937
Size: 566 617 172 1582
Fragments arranged by size:
1582 617 566 172
BbvCI CC'TCA_GC
Cuts at: 0 896 2937
Size: 896 2041
BciVI GTATCCnnnnn_n'
Cuts at: 0 1237 2937
Size: 1237 1700
BfrBI ATG'CAT
Cuts at: 0 543 925 2937
Size: 543 382 2012
Fragments arranged by size:
2012 543 382
BlpI GC'TnA_GC
Cuts at: 0 1613 2937
Size: 1613 1324
BmgBI CAC'GTC
Cuts at: 0 2849 2937
Size: 2849 88
BmrI ACTGGGnnnn_n'
Cuts at: 0 391 1428 2937
Size: 391 1037 1509
Fragments arranged by size:
1509 1037 391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 385 976 1105 1726 1997 2110 2464
Size: 291 94 591 129 621 271 113 354
Cuts at: 2464 2590 2649 2858 2937
Size: 126 59 209 79
Fragments arranged by size:
621 591 354 291 271 209 129 126
113 94 79 59
Bpu10I CC'TnA_GC
Cuts at: 0 896 2937
Size: 896 2041
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 868 1522 2937
Size: 226 642 654 1415
Fragments arranged by size:
1415 654 642 226
BsaI GGTCTCn'nnnn_
Cuts at: 0 1002 2396 2937
Size: 1002 1394 541
Fragments arranged by size:
1394 1002 541
BsaAI yAC'GTr
Cuts at: 0 2932 2937
Size: 2932 5
BsaHI Gr'CG_yC
Cuts at: 0 378 1700 2937
Size: 378 1322 1237
Fragments arranged by size:
1322 1237 378
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2622 2652 2937
Size: 2622 30 285
Fragments arranged by size:
2622 285 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2305 2659 2937
Size: 2305 354 278
Fragments arranged by size:
2305 354 278
BseYI C'CCAG_C
Cuts at: 0 730 1063 2937
Size: 730 333 1874
Fragments arranged by size:
1874 730 333
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1041 2476 2668 2698 2797 2937
Size: 802 239 1435 192 30 99 140
Fragments arranged by size:
1435 802 239 192 140 99 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1702 2937
Size: 1702 1235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 746 989 2690 2937
Size: 746 243 1701 247
Fragments arranged by size:
1701 746 247 243
BsrDI GCAATG_nn'
Cuts at: 0 1078 1448 1608 2937
Size: 1078 370 160 1329
Fragments arranged by size:
1329 1078 370 160
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1482 2937
Size: 1482 1455
BstYI r'GATC_y
Cuts at: 0 1092 1716 2106 2937
Size: 1092 624 390 831
Fragments arranged by size:
1092 831 624 390
BtsI GCAGTG_nn'
Cuts at: 0 56 1480 2840 2852 2937
Size: 56 1424 1360 12 85
Fragments arranged by size:
1424 1360 85 56 12
ClaI AT'CG_AT
Cuts at: 0 2369 2937
Size: 2369 568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 66 101 435 470 639 674 2937
Size: 66 35 334 35 169 35 2263
Fragments arranged by size:
2263 334 169 66 35 35 35
EaeI y'GGCC_r
Cuts at: 0 1658 2306 2418 2937
Size: 1658 648 112 519
Fragments arranged by size:
1658 648 519 112
EarI CTCTTCn'nnn_
Cuts at: 0 512 2937
Size: 512 2425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 385 814 976 1105 1726 1997 2110
Size: 291 94 429 162 129 621 271 113
Cuts at: 2110 2464 2590 2637 2649 2858 2937
Size: 354 126 47 12 209 79
Fragments arranged by size:
621 429 354 291 271 209 162 129
126 113 94 79 47 12
EcoO109I rG'GnC_Cy
Cuts at: 0 2051 2937
Size: 2051 886
EcoRV GAT'ATC
Cuts at: 0 618 2937
Size: 618 2319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 220 2937
Size: 220 2717
FspI TGC'GCA
Cuts at: 0 2030 2937
Size: 2030 907
HaeII r_GCGC'y
Cuts at: 0 1703 2937
Size: 1703 1234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 602 634 822 854 1345 1377
Size: 20 32 550 32 188 32 491 32
Cuts at: 1377 1701 1733 2473 2505 2937
Size: 324 32 740 32 432
Fragments arranged by size:
740 550 491 432 324 188 32 32
32 32 32 32 20
HincII GTy'rAC
Cuts at: 0 474 2328 2937
Size: 474 1854 609
Fragments arranged by size:
1854 609 474
HindIII A'AGCT_T
Cuts at: 0 2216 2937
Size: 2216 721
HpaI GTT'AAC
Cuts at: 0 2328 2937
Size: 2328 609
KasI G'GCGC_C
Cuts at: 0 1699 2937
Size: 1699 1238
MfeI C'AATT_G
Cuts at: 0 1679 2937
Size: 1679 1258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1317 2937
Size: 1317 1620
MscI TGG'CCA
Cuts at: 0 1660 2308 2420 2937
Size: 1660 648 112 517
Fragments arranged by size:
1660 648 517 112
MslI CAynn'nnrTG
Cuts at: 0 719 1961 2285 2937
Size: 719 1242 324 652
Fragments arranged by size:
1242 719 652 324
MspA1I CmG'CkG
Cuts at: 0 730 1235 1311 2866 2878 2937
Size: 730 505 76 1555 12 59
Fragments arranged by size:
1555 730 505 76 59 12
NarI GG'CG_CC
Cuts at: 0 1700 2937
Size: 1700 1237
NdeI CA'TA_TG
Cuts at: 0 1500 2937
Size: 1500 1437
NsiI A_TGCA'T
Cuts at: 0 545 927 2937
Size: 545 382 2010
Fragments arranged by size:
2010 545 382
NspI r_CATG'y
Cuts at: 0 965 1323 2140 2937
Size: 965 358 817 797
Fragments arranged by size:
965 817 797 358
PasI CC'CwG_GG
Cuts at: 0 1034 2493 2937
Size: 1034 1459 444
Fragments arranged by size:
1459 1034 444
PciI A'CATG_T
Cuts at: 0 2136 2937
Size: 2136 801
PflMI CCAn_nnn'nTGG
Cuts at: 0 387 2553 2937
Size: 387 2166 384
Fragments arranged by size:
2166 387 384
PfoI T'CCnGG_A
Cuts at: 0 701 1983 2606 2937
Size: 701 1282 623 331
Fragments arranged by size:
1282 701 623 331
PmlI CAC'GTG
Cuts at: 0 2932 2937
Size: 2932 5
PpuMI rG'GwC_Cy
Cuts at: 0 2051 2937
Size: 2051 886
PsiI TTA'TAA
Cuts at: 0 719 2937
Size: 719 2218
PstI C_TGCA'G
Cuts at: 0 51 158 933 1316 2883 2937
Size: 51 107 775 383 1567 54
Fragments arranged by size:
1567 775 383 107 54 51
PvuII CAG'CTG
Cuts at: 0 730 1235 1311 2866 2878 2937
Size: 730 505 76 1555 12 59
Fragments arranged by size:
1555 730 505 76 59 12
ScaI AGT'ACT
Cuts at: 0 799 1897 2937
Size: 799 1098 1040
Fragments arranged by size:
1098 1040 799
SfcI C'TryA_G
Cuts at: 0 47 131 154 929 1312 1440 2879 2937
Size: 47 84 23 775 383 128 1439 58
Fragments arranged by size:
1439 775 383 128 84 58 47 23
SfoI GGC'GCC
Cuts at: 0 1701 2937
Size: 1701 1236
SmlI C'TyrA_G
Cuts at: 0 205 847 1537 2937
Size: 205 642 690 1400
Fragments arranged by size:
1400 690 642 205
SphI G_CATG'C
Cuts at: 0 965 2937
Size: 965 1972
SspI AAT'ATT
Cuts at: 0 2014 2937
Size: 2014 923
StuI AGG'CCT
Cuts at: 0 2183 2830 2937
Size: 2183 647 107
Fragments arranged by size:
2183 647 107
StyI C'CwwG_G
Cuts at: 0 1108 1694 1817 2666 2937
Size: 1108 586 123 849 271
Fragments arranged by size:
1108 849 586 271 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2311 2937
Size: 2311 626
TatI w'GTAC_w
Cuts at: 0 464 797 1895 2937
Size: 464 333 1098 1042
Fragments arranged by size:
1098 1042 464 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2243 2275 2844 2876 2937
Size: 2243 32 569 32 61
Fragments arranged by size:
2243 569 61 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1000 1701 2316 2937
Size: 1000 701 615 621
Fragments arranged by size:
1000 701 621 615
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApoI AvaI BaeI
BamHI BanI BbeI BbsI BbvCI BciVI BfrBI BlpI
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsmBI BspMI BsrDI BstAPI
BstYI BtsI ClaI CspCI EaeI EarI Eco57MI EcoO109I
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI MfeI MmeI MscI MslI MspA1I NarI NdeI
NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI
PsiI PstI PvuII ScaI SfcI SfoI SmlI SphI
SspI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BanII BcgI BclI BglI BglII Bme1580I BmtI BplI
BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI FseI FspAI KpnI
MluI NaeI NcoI NgoMIV NheI NotI NruI PacI
PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI